S182

GASTROINTESTINAL HORMONES CONCENTRATIONS IN DOG PLASHA IN PANCREATIC ATROPHY. V.B.Troitekaya, V.L.Kuznetsov, A.I. Panin, N.S.T~ck. I.P.Pa~1ovlnstitute of Physiology, Leningz~md, USER; Toronto University, Canada. Immunoreective pancreatic polypeptide (PP), gastI~n end motilin concent~- tlonm were measured in pg/ml in plasma o? 4 doge during 31 month of pan- creatic atrophy development, produced by the obstruction of the outflow of t h e i r pancreatic ju ice. Basal PP and gas t~n plasma concentrations decreased beginning with the 21st month in comparison with the normal con- ditions (PP - from 249.33 to ?6.D pg/ml, p~ 0.02; gestz~n - from ?D.5 to 3.B3 pg/ml, p (0.01), but their postprandial response v~s maintained. The postprandial response of PP and gast~n was more pronounced after the be- ginning of periodic subcutaneous injections of boiled pancreatic juice. Basel plasma motilin concentration did not change. For the first time the increase of canine plasma motilln concentration v~s demonstrated postpren- dlally both before and in the pancreatic atrophy, In the pancreatic atz~phy the ratio of PP, gastrln and motilln concentrations changed. In normal con- ditions and before the I2th month of pancreatic atrophy development basel and postprandial PP concentrations exceeded motilin concentrations; begin- ning with the I2th month both basal and postprandial concentrations of mo- tilin became higher then those of PP (p< 0.01). The correlations between the concentrations of individual hormones as well as the dlfferences and the retios of the concentrations of some pairs of hormones not observed in intact animals were revealed in pancreatic atrophy, In pancreatic atro- phy in the digestive process the connections between the differences and the ratios of the concentrations of some pairs of hormones were found, They were not displayed between the concentrations of individual hormones. The results suggest that the regulation of the differences and the ~tios between hormone concentretlons may play an essential pert in the mainte- nance of postprandlal hormone response in pancreatic atrophy, It was also shown that the maintenance of postprendlal hormone response during pan- creet~ic atrophy development appears to be performed by the regulation of the rates of the hormonal concentrations changes and not by the changes of their absolute values.

DECREASED CCK-8 IMMUNOREACTIVlTY IN BRAIN OF RATS WITH EXPERIMENTALLY INDUCED LIVER CIRRHOSIS. A. Malesci, C. Bonato, F. Baldissera, A.E. Panerai and F. Salerna I s t i t u t o di Clinica Medica I I I Universit~ di Milano, I t a l y .

We recently reported that beta-endorphin hypothalamic immunoreactivity is decreased in rats with experimental l i v e r c i r rhos is . In the same animals unaltered concentrations of met-enkephalin, substance P and somatostatin were recovered from brain extracts. Since beta-endorphin as well as chole- cys tok in in- l i ke peptides have been suggested to be involved both in pain modulation and feeding control we determined the concentration of these two classes of neuropeptides in the same experimental model. In 9 Sprague- Dawley male rats l i v e r c i r rhosis was induced by CCI 4 intraperi toneal in- jections (0.08 ml in sesame o i l per 100 g of weight} repeated every three days over 9 weeks. Ten rats of the same age and sex underwent sesame o i l inject ions by the same schedule. Animals were k i l l ed by microwave i r rad ia - t ion and brain areas extracts were assayed for beta-endorphin and CCK-8 immunoreactivity by speci f ic RIAs. In rats with c i r rhosia beta-endorphin immunoreactivity was confirmed to be s ign i f i can t l y reduced (p < 0.01) only in the hypothalamus whereas i t was unmodified in the other brain areas.

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On ~he other hand CCK was s lgn l f l can t l y reduced In the cortex (21 - 3 vs. 44 bng per mg of proteins; p 0.01). Although not s t a t i s t i c a l l y s ign i -