s211 431 EXPRESSION OF MASH1 IN TASTE EPITHELIUM IS DEPENDENT UPON GUSTATORY INNERVATION YUJI SETA, SHINOBU TAKEDA, AND KUNIAKI TOYOSHIMA Department of Oral Anatomy 11, Kyushu Dental College, Kokurakita-ku, Kitakyushu 803-8580 Mushl, a mammalian homologue of Drosophila achaete-s&e proneural gene complex, plays an essential role in differentiation of subsets of peripheral neurons. In this study, using RT-PCR and in situ hybridization, we investigated if Mash1 gene expression occurs in rat taste buds. Further, we examined dynamics of Mash1 expression in the process of degeneration and regeneration in denervated rat taste buds. In rat tongue epithelium, Mash1 gene expression is confined to circumvallate, foliate, and fungiform papillae epithelia that include taste buds. In taste buds, Mashl-expressing cells are round cells in the basal compartment. In contrast, the mature taste bud cells don’t express the Mash1 gene. Denervation and regeneration expriments show that the expression of ,Mashl requires gustatory innervation. We conclude that Mush1 is expressed in cells of the taste bud lineage, and that the expression of Mash1 in rat taste buds is dependent upon gustatory innervation.

Expression of Mash1 in taste epithelium is dependent upon gustatory innervation

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s211

431 EXPRESSION OF MASH1 IN TASTE EPITHELIUM IS DEPENDENT UPON

GUSTATORY INNERVATION

YUJI SETA, SHINOBU TAKEDA, AND KUNIAKI TOYOSHIMA

Department of Oral Anatomy 11, Kyushu Dental College, Kokurakita-ku, Kitakyushu 803-8580

Mushl, a mammalian homologue of Drosophila achaete-s&e proneural gene complex, plays an essential role in differentiation of subsets of peripheral neurons. In this study, using RT-PCR and in situ hybridization, we investigated if Mash1 gene expression occurs in rat taste buds. Further, we examined dynamics of Mash1 expression in the process of degeneration and regeneration in denervated rat taste buds. In rat tongue epithelium, Mash1 gene expression is confined to circumvallate, foliate, and fungiform papillae epithelia that include taste buds. In taste buds, Mashl-expressing cells are round cells in the basal compartment. In contrast, the mature taste bud cells don’t express the Mash1 gene. Denervation and regeneration expriments show that the expression of ,Mashl requires gustatory innervation.

We conclude that Mush1 is expressed in cells of the taste bud lineage, and that the expression of Mash1 in rat taste buds is dependent upon gustatory innervation.