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Biochemical
Histological Imaging
0
2
4
6
8
10
12
14
16
0 2 4 6 8 10 12 14
ATP
fm
ol/
cell
cultivation time (days)
WE, FCS
WE, FCS, 0.2% DMSO
WE, FCS, Trophic Factor
0
200
400
600
800
1000
1200
1400
1600
1800
2000
FCS FCS, 0.2% DMSO FCS, Trophicfactor
RLU
/MT
not induced
induced
0
500
1000
1500
2000
2500
3000
FCS FCS, 0.2% DMSO FCS, Trophicfactor
RLU
/MT
not induced
induced
0.00
0.02
0.04
0.06
0.08
0.10
0.12
0.14
0.16
0.18
0.20
2 3 4 6 7 10
RLU
/ce
ll
cultivation time (days)
rLiMT 2D monolayer
0
1
2
3
4
5
6
7
8
9
10
Hep Hep/NPC 1:1 2D sandwich
Alb
um
in p
g/(
cell*
h) day 4
day 7
day 9
0
500
1000
1500
2000
2500
3000
3500
FCS, 0.2% DMSO, Trophic factor
RLU
/MT
not induced induced
0
2
4
6
8
10
12
14
16
18
10 15
Alb
um
in [
pg/
(h*c
ells
)]
Cultivation time (days)
FCS
FCS, 0.2% DMSO
FCS, Trophic factor
