Equine Health Studies Program 2008-2010 Equine Research …Equine Health Studies Program 2008-2010 Equine Research Report. Dr. Peter F. Haynes Dean ... and surgery that will impact

Scientific studies conducted to help advance equine health and well-being

Equine Health Studies Program

2008-2010 Equine Research Report

Dr. Peter F. HaynesDean

Dr. James E. MillerInterim Associate Dean for Research and Advanced Studies

Dr. Joseph TaboadaAssociate Dean for Student and Academic Affairs

LSU SCHOOL OF VETERINARY MEDICINE ADMINISTRATION

Dr. David F. SeniorAssociate Dean for Advancement and Strategic Initiatives

Ernie TanoosAssistant Dean for Finance and Administrative Services

LETTER FROM OUR DEAN

The Louisiana State University School of Veterinary Medicine is pleased to once again present the Equine Health Studies Program’s Equine Research Report, which covers scientific activities of the program from 2008 through 2010. Central to the program’s mission is the health, well-being and performance of horses supported through state-of-the-art research that benefits the horse-owning public in Louisiana and beyond. As a former equine surgeon and faculty member, I have watched the EHSP grow and flourish, as evidenced by contents of this Research Report, translating research into practical solutions for our broad-base constituents and clients. In addition to its research prowess, the program’s dedicated faculty and staff provide clinical service, education, and community outreach.

The EHSP has made significant advances in research collaborations with industry to extend its work in the areas of laminitis prevention; lameness, orthopedics and biomechanics; reproductive disorders; respiratory and gastrointestinal diseases including the treatment and prevention of gastric ulcer disease; equine Cushing’s disease; and surgery that will impact equine veterinary care for years to come.

The EHSP continues to build and maintain strong relationships and community engagement with the stakeholders of Louisiana so that it can be responsive to the needs of horses in the region. In the aftermath of Hurricanes Gustav and Ike and the Gulf Oil Spill, the SVM was able to step in and help with the rescue and care of animals and wildlife in south Louisiana. The EHSP, in concert with the SVM and the Louisiana State Animal Rescue Team, has been on the forefront of emergency preparedness, and continues to be a model for other veterinary institutions. Additionally, the EHSP continues to provide strong leadership within the State and has been actively involved with referring veterinarians, the Louisiana Racing Commission, the Louisiana Horse Council, the Louisiana Thoroughbred Breeders Association, and many other organizations to provide the horsemen with the latest information and quality service.

Through the dedicated efforts of its faculty and staff, and the continued facility and equipment enhancements in support of its activities, this multidisciplinary program is a real “Point of Pride,” reflects a very positive trajectory, and continues to bring national and international recognition to the School.

Sincerely,

Peter F. Haynes, DVM, DACVSDean

Dr. Frank AndrewsDirector of the Equine Health Studies Program

Ky MortensenDirector of Advancement and Client Services

Dr. Margaret CoffeyDirector of the LSU Veterinary Teaching Hospital

Ginger GuttnerDirector of Public Relations

The Louisiana State University School of Veterinary Medicine continues to promote a high level of support for equine health through teaching, research and service. The equine industry is an integral part of the Louisiana livestock community, especially the racing industry. Our Equine Health Studies Program is the basis for the research effort that investigates and provides up to date and pertinent information for teaching students and extending service to the public. It is important for our faculty to continue building through extra-mural support and we have been very fortunate to have a number of sources that include federal (NIH, USDA), state (Equine health appropriation) and private/industry (Grayson, Elanco, Boehringer Ingelheim Vetmedica, Zinpro Corporation, Centaur Corporation, Seabuck Equine, LLC, SmartPill Corporation, Pall Corporation) funding. The faculty contributing to the program are to be commended for their concerted efforts to ensure that equine health remains as one of the top priorities of the School.

Sincerely,

James E. Miller, DVM, MPVM, PhDInterim Associate Dean for Research & Advanced StudiesProfessor of Epidemiology & Community Health

LETTER FROM OUR INTERIM ASSOCIATE DEAN FOR RESEARCH & ADVANCED STUDIES

LETTER FROM OUR DIRECTOR

It’s my pleasure to introduce the 2008-10 Equine Research Report from the Equine Health Studies Program (EHSP), Louisiana State University (LSU) School of Veterinary Medicine (SVM)! As director, I am honored to present this research report documenting the productive faculty here at LSU.

The EHSP team continues to work toward the missions of clinical service, teaching, and research. As you can see in the contents of this report, faculty and staff continue to provide high quality biomedical research to address the health needs of the horse industry in Louisiana, the U.S., and the world. I congratulate each and everyone in the program for their continued commitment and devotion to excellence of the EHSP!

As you read this report, the EHSP biomedical research team at the LSU SVM has diverse research interests with one major goal–to improve the health and welfare of the horse. Investigators represented in this report contributed important information in areas of Emergency Response, Gastrointestinal Disease, Laminitis, Musculoskeletal Injury and Lameness, Orthopedics, Reproduction, Respiratory Physiology and Disease, and Colic Surgery. Each study in this report highlights benefits to the horse industry in Louisiana and surrounding regions. Many of these research findings were presented at national and international meetings, including the American Association of Equine Practitioners, American College of Veterinary Surgery, American College of Veterinary Internal Medicine, British Equine Veterinary Association, World Congress on Disaster and Emergency Medicine, Veterinary Orthopedic Society, The Laminitis Society, Applied Equine Nutrition and Training Conference, South African Equine Veterinary Association, Association Argentina de Veterinaria Equina, and the American Society of Animal Science. As you can see, researchers in the EHSP are sought after speakers at many major conferences throughout the world.

The biomedical research outlined in this report and state-of-the-art facilities on campus are a direct result of support from the Louisiana Governor’s Biotechnology Initiative Grants Program, Louisiana Board of Regents Enhancement Grants Program, and recurrent funding through the State Legislature resulting from Louisiana racetrack slot machine

The Equine Research Report is published by the Louisiana State University, School of Veterinary Medicine, Baton Rouge, LA 70803.

Phone: (225) 578-9500

Fax: (225) 578-9605

[email protected]

revenue. Also, funding for many of these important projects was provided from extramural sources, including the Grayson Jockey Club Research Foundation, the Morris Animal Foundation, Merck-Merial Veterinary Student Summer Research Program, Boehringer-Ingelheim Vetmedica, Pennington Biomedical Research Foundation, Applied Biosystems, Howard Hughes Medical Institute, National Wetlands Research Center, and National Institutes of Health, among others.

Furthermore, we owe our deepest gratitude to the horses that participated in these investigational studies. The research findings presented in this report would not have been possible without the availability and use of horses. All biomedical research on animals at LSU is conducted under Federal Guidelines for the Humane Care and Use of Animals and approved by the Institutional Animal Care and Use Committee (IACUC) within the SVM. Horses were carefully and compassionately used for the advancement of equine health to discover more effective methods to diagnose, treat, and prevent illness and injuries. These horses are valued members of our program and are treated with kindness and dignity. In some cases, data obtained from medical records may have been used to identify specific risk factors and treatment outcomes for important diseases or injuries. All client and animal identity was kept in strict confidence and will not be released under any circumstances.

The faculty and staff of the EHSP are proud to present this research report and look forward to the continued participation in the biomedical research program as it moves forward. As part of the EHSP team, I am committed to furthering the health, well-being and performance of horses to the benefit of the people and horses of Louisiana. Also, with continued support from the horse industry and the State of Louisiana, we can further promote and establish the EHSP at the LSU SVM as the premier equine biomedical program in the world.

Sincerely,

Frank M. Andrews, DVM, MS, DACVIM

Professor and Director

LSU School of Veterinary Medicine on Facebook: http://www.facebook.com/LSU SVM

LSU EHSP on Facebook: http://www.facebook.com/LSUequine

LSU School of Veterinary Medicine on Twitter: http://www.twitter.com/LSUVetMed

http://www.vetmed.lsu.edu

Our Mission: The LSU Equine Health Studies Program will become a premier equine biomedical center in the 21st century through leading-edge research of equine diseases, contemporary instruction of professional veterinary students and veterinarians in advanced studies programs, and enhanced continuing education of the horse-owning public and private equine practitioners, with the ultimate goal of providing state-of-the-art diagnostic and therapeutic capabilities for critically ill and injured horses, and optimal clinical service to horsemen in Louisiana and the surrounding region.

TABLE OF CONTENTS

2008-2010 EHSP FACULTY ............................................................................................................ 4

2008-2010 PUBLICATIONS AND ABSTRACTS .......................................................................... 12

2008-2010 GRANTS ...................................................................................................................... 30

EHSP FACILITIES AND EQUIPMENT .......................................................................................... 36

EHSP DEVELOPMENT ................................................................................................................. 46

RESEARCH NEWS & DEVELOPMENT ........................................................................................ 48

BIOMECHANICS AND ORTHOPEDICS ....................................................................................... 50

Biomechanical algorithm to quantify forces from equine motion at the saddle position ...... 50

An in vitro biomechanical comparison between dynamic compression plates having a roughened contact surface and dynamic compression plates having a polished contact surface for the fixation of osteotomized equine third metacarpal bones ............................. 52

An In Vitro Cyclic Fatigue Comparison of a Modified 5.5 mm Locking Compression Plate Fixation with a 5.5 mm Locking Compression Plate Fixation of Osteotomized Equine Third Metacarpal Bones ................................................................................................................ 53

STEM CELL AND REGENERATIVE MEDICINE .......................................................................... 54

In Vitro Tissue Generation by Adult Equine Multipotent Stromal Cells on Collagen Scaffolds 54

The Equine Health Studies Program is supported with funds provided by the Louisiana State University School of Veterinary Medicine, the State of Louisiana, and contributions from private donors.

http://www.equine.vetmed.lsu.edu

TABLE OF CONTENTS

LAMINITIS ...................................................................................................................................... 56

Isolation and characterization of equine laminar progenitor cells ........................................ 56

Treatment of Experimental Equine Laminitis with Doxycycline .......................................... 58

GASTROINTESTINAL ................................................................................................................... 59

Pharmacokinetics and clinical safety of oral paste firocoxib, a COX-1 sparing NSAID, in foals ..................................................................................................................................... 59

Right Colonic Pathophysiology in Horses Administered Phenylbutazone ........................... 59

New Approaches in Treating Equine Gastric Ulcer Syndrome ............................................ 60

DISASTER RESPONSE ................................................................................................................ 62

Experiential learning in disaster response for veterinary students and veterinarians .......... 62

SURGERY ...................................................................................................................................... 63

LARMoF’s Procedure for Treatment of Cribbing in Horses: Is it working? .......................... 63

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2008-2010 EHSP FACULTY AND STAFF

Frank M. Andrews, Professor and Director Equine Health Studies Program, Veterinary Clinical Sciences

Dr. Andrews received his DVM and MS in Veterinary Medicine from Washington State University in 1983. He completed 1 year in

private practice at Associated Veterinary Clinics, Inc. in Walla Walla, Washington and completed an internal medicine residency in

1988 at The Ohio State University, College of Veterinary Medicine. Dr. Andrews became board certified in Large Animal Internal

Medicine in 1989 and was on the faculty at the University of Tennessee, College of Veterinary Medicine from 1988-2008. Dr.

Andrews is currently the LVMA Equine Committee Professor and Director of the Equine Health Studies Program. Dr. Andrews’

research focuses on investigating treatment and prevention of gastric ulcer disease, gastric emptying and gastrointestinal motility,

and treatment of pituitary par intermedia dysfunction (Equine Cushing’s Disease).

Steven A. Barker, Professor and Director Equine Medication Surveillance Laboratory, Comparative Biomedical Sciences

Dr. Barker is a professor of veterinary physiology, pharmacology and toxicology at the LSU School of Veterinary Medicine in the

Department of Comparative Biomedical Sciences. He received his BS in 1971, his MS in 1973, and his PhD in 1978, all from the

University of Alabama in Birmingham. Dr. Barker is also the director of the Analytical Systems Laboratory and the director of the

Equine Medication Surveillance Laboratory. He has served as the State Chemist to the Louisiana State Racing Commission since

1987.

Ralph E. Beadle, Professor Emeritus, Equine Medicine, Veterinary Clinical Sciences

Dr. Beadle was born and raised in Montana. He completed his pre-veterinary and veterinary education at Colorado State

University, where he was awarded a DVM in 1967. He spent the next five years at the University of Georgia, where he worked in

the Equine Clinic and obtained a PhD in Veterinary Physiology. After a period of two years spent at Michigan State University, he

has been at LSU for the rest of his professional career. During the first seven years at LSU, he was in the Department of Veterinary

Physiology, Pharmacology and Toxicology, where he taught both physiology and pharmacology. From that time until September

1999, he was in the Department of Veterinary Clinical Sciences, where he worked in the Medicine Section of the Equine Clinic. He

retired in September 1999, but since that time has continued to be involved in the activities of the Department of Veterinary Clinical

Sciences as a professor emeritus. His research interests involve non-sweating horses, horses with recurrent obstructive airway

disease, and horses affected with acute and chronic laminitis.

Hermann H. Bragulla, Assistant Professor, Comparative Biomedical Sciences

Dr. Bragulla received his DVM (1984) and PhD (1986) in Veterinary Medicine from the Ludwig-Maximilians-University in Munich/

Germany. He became a certified specialist for veterinary anatomy (1991) and received his habilitation (1996) from the Free

University Berlin in Berlin/Germany. Dr. Bragulla was on the faculty of the Department of Veterinary Anatomy, School of Veterinary

Medicine, Free University Berlin, from 1988 – 2004. He started as an instructor (1988 – 1996) and was promoted to assistant

professor (1996 – 2004). Dr. Bragulla’s research focuses on the comparative and developmental morphology of digital end organs

such as the equine hoof concerning the age-related normal structure versus disease-related changes in the structure of keratinized

tissues and changes in the papillary body and blood supply of connective tissues of digital end organs.

Daniel J. Burba, Professor Equine Surgery, Veterinary Clinical Sciences

Daniel J Burba was born in west central Pennsylvania. He completed an undergraduate study in Biology at Morehead State

University, Morehead, KY and attended the College of Veterinary Medicine at Auburn University. Dr Burba was accepted into an

internship program in Large Animal Medicine and Surgery at Oklahoma State University and subsequently completed a residency

in Equine Surgery in 1990. Dr Burba became a Diplomate of the American College of Veterinary Surgeons in 1995. He has

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received such rewards as the Carl J. Norden distinguished teaching award, teaching merit honor roll of Gamma Sigma Delta Honor

Society of Agriculture. Dr. Burba has published numerous book chapters on equine wound management, as well as several journal

articles and abstracts. He is a member of Phi Zeta Veterinary Medicine Honor Society, Gamma Sigma Delta Agriculture Honor

Society, Veterinary Wound Management Society, American Association of Equine Practitioners, American Veterinary Medical

Association, and American Association of Veterinary Clinicians. Dr Burba’s main interest is in orthopedics and laser cribbing

surgeries.

Renee T. Carter, Assistant Professor Ophthalmology, Veterinary Clinical Sciences

Dr. Carter received her DVM in Veterinary Medicine from Louisiana State University in 2000 and completed a rotating internship

in small animal medicine and surgery at LSU in 2001. She completed a four-year comparative ophthalmology residency and

fellowship at the University of Madison-Wisconsin in 2006 and returned to LSU as an assistant professor in Veterinary Clinical

Sciences (VCS). Dr. Carter became board certified in Veterinary Ophthalmology in 2007 and her research focuses on corneal

wound healing disorders and the role of Leptospirosis in the pathogenesis of equine recurrent uveitis.

Ann Chapman, Assistant Professor Equine Medicine, Veterinary Clinical Sciences

Dr. Chapman was born and raised in Harrisburg, Penn. She received her BA in Biology from Gettysburg College in 1990 and

worked in laboratory research for both private and government companies. She received her DVM from the LSU School of

Veterinary Medicine in 2001. After working in private practice for one year, she began her combination Equine Internal Medicine

Residency/Graduate Program at LSU in 2002. Dr. Chapman completed her residency in 2005 and became a Diplomate of the

American College of Veterinary Internal Medicine (Large Animal). She received a Masters of Science in Veterinary Clinical

Sciences in December 2006. She performs Mobile Equine Medicine Consultation throughout the state of Louisiana and Equine

Ambulatory Medicine to the local Baton Rouge community.

Shafiqul I. Chowdhury, Professor Molecular Virology, Pathobiological Sciences

Dr. Shafiqul Chowdhury received his DVM (1978) and MS (1980) in Veterinary Medicine from Bangladesh Agricultural in University

in 1978. He received his PhD in 1987 from Free University of Berlin, Germany. Dr. Chowdhury became Full Professor in the

College of Veterinary Medicine, Kansas State University in 2002. He joined LSU School of veterinary medicine in 2008. Dr.

Chowdhury’s research focuses on molecular pathogenesis of equine herpes type 1 (EHV-1) neuropathogenesis and development

of an efficacious vaccine against EHV-1. His EHV-1 research is funded by a corporate grant.

Anderson daCunha, Assistant Professor Anesthesiology, Veterinary Clinical Sciences

Dr. da Cunha is an assistant professor of veterinary anesthesiology in the Department of Veterinary Clinical Sciences. He is also

service chief of Anesthesia Services in the Veterinary Teaching Hospital & Clinics. Dr. da Cunha received his DVM from Federal

University of Parana in 2000, his MS from Federal University of Santa Maria in 2002 and after his residency at North Caroline State

University (2006) he became a diplomate of the American College of Veterinary anesthesiologists in 2009.

Fabio Del Piero, Professor of Pathology, Pathobiological Sciences

Dr. Del Piero started his career in pathology in the late eighties and worked at University of Milano, Animal Health Trust UK, Cornell

University, and University of Pennsylvania. He joined LSU in 2012 and he is currently Professor of Pathology in the Department of

Pathobiological Sciences. Dr. Del Piero investigations focus on comparative pathology with emphasis on equine pathology, and

infectious diseases.

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Susan C. Eades, Professor Equine Medicine, Veterinary Clinical Sciences

Dr. Eades graduated from the LSU School of Veterinary Medicine in 1982, then completed an internship in large animal medicine

and surgery and a residency in large animal internal medicine at the University of Pennsylvania’s New Bolton Center. She then

completed a PhD in Veterinary Physiology at the University of Georgia. Her doctoral studies concentrated on intestinal vascular

and nonvascular smooth muscle physiology and pharmacology. Upon completion of her PhD, Dr. Eades began as an assistant

professor of large animal medicine at the University of Georgia College of Veterinary Medicine, where she remained through 1997.

She returned to LSU in 1998 as an associate professor of equine medicine. Dr. Eades’ clinical interests include equine internal

medicine; however, she has a special interest in cardiology and ultrasound. Her research interests include intestinal disease and

laminitis.

Bruce E. Eilts, Professor Theriogenology, Veterinary Clinical Sciences

Dr. Eilts is originally from the Minneapolis/St. Paul area in Minnesota. He graduated from high school in West St. Paul, Minn.,

and then attended the University of Minnesota as a pre-veterinary medicine student. He obtained a BS in veterinary science

in 1975 and his DVM in 1977, both from the University of Minnesota. He was in private practice for one year before returning

to the University of Minnesota to obtain an MS in theriogenology in 1982. After two and a half years in a private practice in

southern California, he came to LSU as an assistant professor in 1984. He became board certified in the American College of

Theriogenologists in 1986. His main clinical interest and research is basic reproduction management in the horse.

Joseph Francis, Associate Professor, Comparative Biological Sciences

Dr. Francis is an associate professor in the Department of Comparative Biomedical Sciences. He received his BVSc and his MVSc

in 1990 and 1994, respectively, both from Madras Veterinary College (India). In 1999, Dr. Francis received his PhD from Kansas

State University. His research focuses on the brain mechanisms regulating cardiovascular function, specifically the understanding

of the central nervous system interactions of the cytokine renin-angiotensin-aldosterone system in heart failure.

Lorrie Gaschen, Associate Professor Radiology, Veterinary Clinical Sciences

Dr. Gaschen received her BS and DVM degrees from the University of Florida in 1985 and 1990, respectively. She received

her PhD from the University of Utrecht in the Netherlands in 2001. She is a Diplomate of Diagnostic Imaging and a European

Specialist in Veterinary Diagnostic Imaging. Dr. Gaschen is a Professor and service chief of Diagnostic Imaging in the LSU School

of Veterinary Medicine’s Veterinary Teaching Hospital & Clinics. She joined the faculty at LSU in January 2006. Dr. Gaschen’s

research interests are in vascular imaging and ultrasound of the gastrointestinal tract and pancreas and MRI.

Marjorie S. Gill, Professor Farm Animal Services, Veterinary Clinical Medicine

Dr. Gill received her DVM and MS degrees from Iowa State University in 1976 and 1984, respectively. She is a Diplomate of the

American Board of Veterinary Practitioners (Food Animal Practice). She joined the faculty at LSU in July 1984. Dr. Gill’s clinical

interests are in farm animal medicine, small ruminant medicine and surgery, animal behavior, and urogenital, gastrointestinal and

ophthalmological surgery.

Jeff Gimble, Professor, Pennington Biomedical Research Center

Dr. Gimble is a professor at the Pennington Biomedical Research Center and an adjunct professor in the Department of Veterinary

Clinical Sciences. He received his BA from Dartmouth College in 1976. He received his MA, PhD, and MD from Yale University in

1980, 1981, and 1982, respectively. Dr. Gimble collaborates on numerous adult stem cell projects within the EHSP.


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Samithamby Jeyaseelan, Associate Professor and Director of Lung Biology Laboratory, Pathobiological Sciences

Dr. Jeyaseelan received his DVM degree in Veterinary Medicine from the University of Peradeniya, Sri Lanka in 1992. He

completed his PhD degree in Pulmonary Immunology at the College of Veterinary Medicine, University of Minnesota in 2001. He

completed his first postdoctoral training at Yale University in Pulmonary Immunology from 2001-2003 and second postdoctoral

training at National Jewish Health/Colorado Health Sciences Center in lung Biology from 2003-2004. Dr. Jeyaseelan was on the

faculty at National Jewish Health/Colorado Health Sciences Center from 2004-2007. Dr. Jeyaseelan is currently an Associate

Professor of Immunology in Pathobiological Sciences and Director of the Lung Biology Laboratory. Dr. Jeyaseelan’s research

focuses on investigating the mechanisms underlying lung inflammation and host defense in response to bacterial pathogens. Dr.

Jeyaseelan has published more than 40 original articles and more than 12 review articles. He serves as an Associate Editor of the

Journal of Immunology, Academic editor of PLoS One and editorial boards of Clinical and Vaccine Immunology and Infection and

Immunity. Dr. Jeyaseelan’s research program is funded by the National Institutes of Health and Flight Attendant Medical Research

Institute.

Jill R. Johnson, Professor Emeritus Equine Medicine, Veterinary Clinical Sciences

Dr. Johnson is a native of South Dakota. She graduated from veterinary school at the University of Minnesota, then stayed on and

completed her MS degree in Veterinary Surgery and Radiology. She joined the faculty of the LSU School of Veterinary Medicine

in 1977. She is a specialist in internal medicine (Diplomate, American College of Veterinary Internal Medicine) and equine

practice (Diplomate, American Board of Veterinary Practitioners). Past research activities have centered on immunogenetics and

immunology, methods of quantifying exercise training using the global positioning system (GPS) and development of tissue culture

models to study diseases. Current research involves the spatial-temporal study of hospital-acquired infections in the large animal

hospital.

Michael T. Kearney, Biostatistician and Instructor, Pathobiological Sciences

Michael received his MS and MApStat degrees from LSU in 1980 and 1981, respectively. He manages statistical consulting for the

School of Veterinary Medicine with primary emphasis on linear models, repeated measures analyses, and nonparametric statistics.

He teaches graduate courses in Biostatistics and using SAS to analyze research data. He also offers a professional curriculum

elective for veterinary statistics.

Konstantin G. Kousoulas, Professor and Director BIOMMED, Pathobiological Sciences

Dr. Kousoulas is a professor of veterinary virology in the department of Pathobiological Sciences at the School of Veterinary

Medicine. He is also a professor of veterinary science in the LSU Agricultural Experiment Station, an adjunct professor of biological

sciences in the College of Basic Sciences and an adjunct professor in the Department of Microbiology and Immunology at the

LSU Health Sciences Center in New Orleans. Dr. Kousoulas is the director of the LSU School of Veterinary Medicine’s Division

of Biotechnology & Molecular Medicine. He received his BS in 1975 from Fairleigh Dickinson. In 1977, he received his MS and in

1981, he received his PhD, both from Pennsylvania State University. Dr. Kousoulas’ research interests are in molecular biology

and pathogenesis of herpes and corona viruses; experimental approaches in his laboratory involve advanced molecular biology,

molecular genetics and cell biology to study viral attachment, viral penetration into cells, cellular receptors and virus spread. Other

interests include the development of vaccines to combat animal and human viral infections, the application of viral vectors for gene

therapy, and the utilization of computers for biological research and teaching.


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Jose A. Len, Theriogenology Instructor, Veterinary Clinical Sciences

Dr. Len received his DVM in veterinary medicine from the Universidad de Guadalajara, Mexico in 1994. He then worked as the

Panamanian Racing Commission Veterinarian at the Hipodromo Presidente Remon Racetrack in Panama City, Rep. of Panama

until 1999. From 1999 to 2005, Dr. Len was the veterinarian and manager of Haras Cerro Punta, a thoroughbred breeding farm in

the Republic of Panama. Dr. Len completed a theriogenology residency in 2008 at Louisiana State University, School of Veterinary

Medicine and became board certified in Theriogenology in 2009. Currently he is an instructor in Theriogenology and PhD candidate

at Louisiana State University, School of Veterinary medicine. Dr. Len’s research is focused in the improvement of processes for

semen chilling and freezing and efficient use of spermatozoa for intracytoplasmic sperm injection (ICSI) in the horse.

Mandi J. Lopez, Associate Professor and Director, Laboratory for Equine and Comparative Orthopedic Research, Veterinary Clinical Sciences

Dr. Lopez is from the Pacific Northwest. She received her bachelor’s degree from Humboldt State University in Arcata, California

and attended veterinary school at the University of California, Davis. She completed a food animal internship at Kansas State

University prior to going to the University of Wisconsin, where she completed a residency in large animal surgery and obtained

MS and PhD degrees. She then did a post-doctoral fellowship in applied biomechanics. Her areas of interest are comparative

orthopedic research and surgery. Dr. Lopez is board certified by the American College of Veterinary Surgeons. Dr. Lopez has

several patents for biomedical devices and has expertise in both applied and basic research. She came to LSU in January 2004

and directs the Laboratory of Equine and Comparative Orthopedic Research (LECOR).

Sara K. Lyle, Assistant Professor Theriogenology, Veterinary Clinical Sciences

Dr. Lyle was born and raised in Gainesville, Fla. She obtained her BS in Chemistry at Duke University and her DVM from the

University of Florida. She completed a residency in theriogenology in 1990 and her MS in reproduction in 1991 at the University

of Florida. She is board certified by the American College of Theriogenologists. She joined LSU in 2002 and completed a PhD in

2008, and is currently an Assistant Professor. Her clinical interests include mare infertility and assisted reproductive technologies.

Her research interests include reproductive immunology (equine) and assisted reproductive technologies in horses.

Charles T. “Chuck” McCauley, Assistant Professor Equine Surgery, Veterinary Clinical Sciences

Dr. McCauley joined the equine faculty in the Department of Veterinary Clinical Sciences in February 2006. Prior to joining

the faculty at LSU, Dr. McCauley was employed in a busy private referral practice in northeast Texas. He completed his BS

in Microbiology and his DVM at Texas A&M University. He successfully completed an internship and residency in food animal

medicine and surgery at Oklahoma State University. In addition, Dr. McCauley completed a residency in large animal surgery

(equine emphasis) at Purdue University. He is double boarded by the American Board of Veterinary Practitioners (Food Animal

Practice) and the American College of Veterinary Surgeons (Large Animal Surgery).

Rebecca S. McConnico, Associate Professor Equine Medicine, Veterinary Clinical Sciences

Dr. McConnico is originally from north central Ohio, where she lived for 18 years. She obtained her BS in Animal Science from

the University of Arkansas, her DVM from Louisiana State University, and her PhD and clinical residency in large animal internal

medicine from North Carolina State University. She is board certified in Large Animal Internal Medicine and her clinical interests

include equine critical care and internal medicine. She is also a certified veterinary acupuncturist. The long term goals of Dr.

McConnico’s research collaborations are elucidating the pathophysiologic mechanisms associated with intestinal diseases in

horses and determining the link between these diseases and other related abnormalities (eg., laminitis, endotoxemia, myositis),

with the broader intention of preventing, attenuating, and determining effective treatment modalities for these life-threatening

conditions. Additionally, Dr. McConnico is integrally involved in the development of the LSU-School of Veterinary Medicine’s


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Disaster program, a training and response program based on community involvement which hinges on growing partnerships with

other LSU units, non-government groups, and state and federal government entities.

Mustajab Mirza, Assistant Professor Equine Surgery and Emergency Service, Veterinary Clinical Sciences

Dr. Mirza received his DVM from the College of Veterinary Sciences Lahore affiliated with the University of Agriculture, Faisalabad,

Pakistan in 1992. He received his MS degree from LSU in 1998. Dr. Mirza’s primary interests are repair of long bone fractures

and pathogenesis of colics in equids, Laminitis, ophthalmology and advanced wound healing. He primarily provides after-hours

emergency services for the LSU Equine Clinic. Dr. Mirza’s research interests are in long bone fractures, performance limitations,

advanced therapeutics for osteoarthritis, and gastrointestinal disease in the horse.

Colin F. Mitchell, Associate Professor Equine Surgery, Veterinary Clinical Sciences

Dr. Mitchell, originally from Perth, Scotland, received his veterinary medical degree from the University of Edinburgh. He then

completed an internship at the University of Prince Edward Island prior to entering a combined three-year equine surgery residency

and MS graduate program at the University of Minnesota, which he completed in June 2004. He then remained on the hospital

staff at the University of Minnesota, where he worked as the equine emergency clinician/surgeon until July 2005, when he joined

the LSU School of Veterinary Medicine. He is board certified by the American College of Veterinary Surgeons. His clinical interests

include orthopedic, laparoscopic and respiratory surgery. His research interests include orthopedic implants and laminitis.

Dale L. Paccamonti, Professor and Head, Veterinary Clinical Sciences

Dr. Paccamonti, originally from Kankakee, Ill., completed his undergraduate and veterinary education at Michigan State University,

receiving his DVM in 1981. After four years in a mixed practice in Chestertown, Md., he pursued advanced training at the University

of Florida, where he completed a residency in theriogenology and received his MS degree in 1988. Dr. Paccamonti is a Diplomate

in the American College of Theriogenologists. He joined the faculty at the LSU School of Veterinary Medicine in 1988, where he is

currently head of the Department of Veterinary Clinical Sciences and a full professor of theriogenology. Dr. Paccamonti’s primary

research interests include the study of infertility in mares, assisted reproduction techniques in horses, factors affecting sperm

motility in stallions, semen cryopreservation in stallions, and the process of fetal maturation and parturition in mares. He also

collaborates in reproductive research in other domestic species. He shares responsibility for clinical theriogenology cases in all

species presented to the Veterinary Teaching Hospital & Clinics.

Daniel B. Paulsen, Professor and Director LADDL, Pathobiological Sciences

Dr. Paulsen received his BS in 1975, his DVM in 1977, and his MS in 1978, all from Kansas State University. In 1989, he received

his PhD from Oklahoma State University. Dr. Paulsen’s major research interests are bovine respiratory disease with emphasis on

Mannheimia haemolytica, Pasteurella multocida, Infectious bovine rhinotracheitis, bovine virus diarrhea, and bovine respiratory

coronavirus; pathogenesis, bacterial genetics, respiratory immunity and vaccinology; toxicologic pathology associated with inhaled

toxins and effects of inhaled substances on the pathogenesis of asthma; and application of immunohistochemical techniques in

equine respiratory disease and laminitis.

Kenneth Pierce, Assistant Professor Ophthalmology, Veterinary Clinical Sciences

Dr. Pierce, a native New Orleanian, received his Bachelor of Science from Tuskegee University in 2001 and his Doctorate of

Veterinary Medicine from Louisiana State University School of Veterinary Medicine in 2005. In 2006 Dr. Pierce completed a Small

Animal Medicine and Surgery internship at the University of Tennessee College of Veterinary Medicine. In 2007 Dr. Pierce traveled

to southern California to complete a specialty ophthalmology internship with Eye Care for Animals and B. Braun, Inc. He then


10

completed a four year Comparative Ophthalmology residency at Michigan State University and Masters degree in 2011. Dr. Pierce

became a Diplomate of the American College of Veterinary Ophthalmologists in August 2011.

Cherie Pucheu-Haston, Assistant Professor Dermatology, Veterinary Clinical Sciences

Dr. Cherie Pucheu-Haston received her DVM from Louisiana State University in 1992, and completed an internship in Small

Animal Medicine and Surgery at LSU in 1993. She received her residency training in veterinary dermatology at North Carolina

State University in 1993-1995, and achieved board certification in Veterinary Dermatology in 1995. She worked as a specialist

in private practice for seven years, then returned to NCSU in 2002 to pursue advanced graduate training. She received her PhD

in Immunology (minor Biotechnology) from NCSU in 2006. She completed three years as a postdoctoral research associate in

the Immunotoxicology Branch of the United States Environmental Protection Agency, as a grantee from the University of North

Carolina-Chapel Hill Curriculum in Toxicology. Dr. Pucheu-Haston returned to LSU in 2011 as an Assistant Professor in Veterinary

Dermatology. Her research interests include identification of factors involved in the development and perpetuation of atopic

dermatitis, the relationships between allergic skin diseases and hypersensitivity in other organ systems (asthma, gastrointestinal

disease) and the identification of genomic or proteomic biomarkers of allergic sensitization.

Nathalie Rademacher. Dr.med.vet., Assistant Professor Radiology

Dr. Rademacher received her DVM from the Justus-Liebig-University in Giessen, Germany in 2000 and her Dr.med.vet. from the

Vetsuisse Faculty in Bern, Switzerland. After completion of 1 year small animal internship in private referral center in Switzerland,

she completed a diagnostic imaging residency in 2006 at the Vetsuisse Faculty, Zurich, Switzerland. Dr. Rademacher became

board-certified in Diagnostic Imaging with the European College of Veterinary Diagnostic Imaging (ECVDI) in 2007 and with the

American College of Veterinary Radiology (ACVR) in 2010. Since 2007, she is employed as an Assistant Professor in Radiology,

tenure track at LSU. Dr. Rademacher’s research focus is ultrasound in small and large animals, contrast enhanced ultrasound of

the pancreas in dogs and cats, elastographic ultrasound application and lung ultrasound in dogs.

Laura M. Riggs, Assistant Professor Equine Surgery, Veterinary Clinical Sciences

Dr. Riggs is originally from Memphis, Tennessee and received a B.S. in biology from Saint Louis University. She received her DVM

from the University of Tennessee College of Veterinary Medicine in 2001. She completed a 1 year large animal internship followed

by a large animal surgery residency at the University of Georgia. In 2007 she received her PhD in veterinary physiology from the

University of Georgia with research studying biomarkers in equine laminitis. Dr. Riggs is board certified by the American College of

Veterinary Surgeons. Dr. Riggs’ research focuses on lameness, laminitis and fracture repair biomechanics.

Gary A. Sod, Assistant Professor Farm Animal Services, Veterinary Clinical Sciences

Dr. Sod received his MA in Mathematics and PhD in Applied Mathematics from the University of California at Berkeley. The next 12

years were spent doing research in mathematical and computational physics, resulting in the writing of a monograph on numerical

methods in fluid dynamics and 42 journal publications. Dr. Sod served as a professor in the Department of Mathematics and an

adjunct professor in the Department of Mechanical Engineering and at Tulane University from 1985 through 1997. Dr. Sod then

attended the LSU School of Veterinary Medicine and obtained his DVM in 2001. He has since completed an equine internship

and food animal medicine and surgery residency at LSU and a large animal surgery residency in conjunction with the Sawtooth

Equine Service in Idaho and LSU. He is presently an assistant professor with the Farm Animal Medicine and Surgery service. Dr.

Sod received the American College of Veterinary Surgeons Research Publication Award in 2004 and 2006, the Mark S. Bloomberg

Memorial Resident Research Award from the Veterinary Orthopedic Society in 2004 and 2005, and the LSU Rainmaker Research

Award in 2009. His research interests include orthopedic surgery, biomechanics and the design of orthopedic implants specific

to the equine patient. He has designed 5 orthopedic plates, 1 interlocking intramedullary pin and 2 screws for use in long bone

fractures and joint arthrodesis in the equine patient. One screw design has been adopted by Acumed for human fracture patients.


11

This work has resulted in the writing of a book on the biomechanics of fractures and fracture repair. He has tested and designed

orthopedic implants for numerous companies, including, Acumed, BioMedtrix, IMEX, Innovative Animal Products, Securos, and

Synthes. Synthes, Inc. has awarded Dr. Sod a 3 year grant for the design of equine specific orthopedic implants.

Eric Storey, Assistant Professor Ophthalmology, Veterinary Clinical Sciences

Dr. Storey is an assistant professor of ophthalmology in the Department of Veterinary Clinical Sciences and a veterinary

ophthalmologist in the Veterinary Teaching Hospital and Clinics. He received his BS and DVM from Auburn University in 1996

and 1999, respectively. Dr. Storey received his MVSc from University of Saskatchewan (Canada) in 2003. He completed

his ophthalmology internship in 2000 at the University of Illinois, and he completed his residency in 2003 at the University of

Saskatchewan. He is a Diplomate of the American College of Veterinary Ophthalmologists. His research interests are in glaucoma,

inherited retinal disease, and equine recurrent uveitis.

Changaram S. Venugopal, Professor, Veterinary Clinical Sciences

Dr. Venugopal is a veterinarian who graduated from Kerala Veterinary College and Research Institute of Kerala University, India.

After practicing as a veterinarian in the Kamadhenu Dairy Farm for five years, he pursued advanced studies and received a Master

of Science degree in neuropharmacology from Calicut University, India. Later he moved to the US for higher studies and received

another MS degree in cardiovascular pharmacology and a PhD in pulmonary pharmacology from the Massachusetts College

of Pharmacy and Allied Health Sciences in a cooperative program with Harvard University in Boston, MA. Then he worked as a

postdoctoral fellow at Harvard Medical School and, in 1981, joined the faculty at LSU School of Veterinary Medicine. He received

the New Investigator Award grant from the National Institutes of Health in 1983 and the Beecham Award for Research Excellence

in 1985. He is a recipient of Faculty Distinguished Scholar Award (2003) from LSU SVM and Distinguished Alumni Award from

his alma mater in 2005. His research interests include recurrent airway obstruction in horses, mediators of airway hyperreactivity,

pathophysiology of insulin resistance in equine laminitis, pharmacology of vasculature, and oxidative stress in equine respiratory

diseases. He is the recipient of research grants as principal investigator from NIH, USDA and Grayson Jockey Research

Foundation and grants from NIEHS and LA Board of Regents as co-PI. In the area of teaching, Dr. Venugopal received honor roll

in merit teaching 1994 to 1999 and the best teacher award (2008) by the class of 2011.


12

TEXTBOOK CHAPTERS

ANDREWS

Nadeau J, Andrews F. Gastric Ulcer Syndrome. In Current Therapy in Equine Medicine Robinson NE, (eds) 6th Edition. Saunders

Co., St. Louis, MO. 366-371, 2009.

Reese RE, Andrews FM. Nutrition and Dietary Management of Equine Gastric Ulcer Syndrome. In Veterinary Clinics of North

America: Equine Practice 25(1):79-92, 2009.

Videla R, Andrews FM. New Perspectives in Equine Gastric Ulcer Syndrome. In Veterinary Clinics of North America: Equine

Practice 25(2):283-302, 2009.

Al Jassim RA, Andrews FM. The bacterial community of the horse gastrointestinal tract and its relation to fermentative acidosis,

laminitis, colic and stomach ulcers. Veterinary Clinics of North America: Equine Practice 25(2):199-215, 2009.

Andrews FM. Cerebrospinal fluid evaluation. In Equine Internal Medicine Reed SM, Bayly WM and Sellon, DC (eds), 3rd Edition

553-556, 2010.

Andrews FM, Lacombe VA. Electrodiagnostic aids and selected neurologic diseases. In Equine Internal Medicine Reed SM, Bayly

WM and Sellon, DC (Eds), 3rd Edition 556-567, 2010.

Andrews FM. Gastric ulcers in horses. The Merck Manual pages 197-198, 2010.

CHAPMAN

Chapman AM. Acute Diarrhea in hospitalized horses. Vet Clin North Am Equine Pract. 2009 Aug;25(2):363-80.

EADES

Eades SC. Overview of current laminitis research. Vet Clin North Am Equine Pract. 2010 Apr;26(1):51-63.

Stokes AM, Eades SC, Moore RM. Pathophysiology and Treatment of Acute Laminitis. Equine Internal Medicine 3rd Edition. Reed,

Bayly, Sellon. 2010.

EILTS

Mateus, L and Eilts, BE. Cystic Endometrial Hyperplasia and Pyometra In: Ettinger S. and Feldman, E (eds). Textbook Of

Veterinary Internal Medicine, 7th ed. 2209 pp. 1913-1921

Eilts, BE. Contraception and pregnancy termination in the dog and cat. In: Ettinger S. and Feldman, E (eds). Textbook Of

Veterinary Internal Medicine, 7th ed. 2209 pp. 1908-1913

Pinto CRF, Eilts BE, Paccamonti DL. Animal Reproduction In: Clinical Textbook for Veterinary Technicians, 7th edition, McCurnin

2008-2010 PUBLICATIONS AND ABSTRACTS

13


D and Bassert J, eds. Harcourt Health Sciences, St. Louis. 2009 370-399

GILL

Gill MS. Acute Renal Failure. In Haskell (ed): Five-Minute Veterinary Consult: Ruminant, 1st edition. Philadelphia, Lippincott,

Williams & Wilkins, March, 2009.

Gill MS. Amyloidosis. In Haskell (ed): Five-Minute Veterinary Consult: Ruminant, 1st edition. Philadelphia, Lippincott, Williams &

Wilkins, March, 2009.

Gill MS. Patent Urachus. In Haskell (ed): Five-Minute Veterinary Consult: Ruminant, 1st edition. Philadelphia, Lippincott, Williams &


Gill MS. Pyelonephritis. In Haskell (ed): Five-Minute Veterinary Consult: Ruminant, 1st edition. Philadelphia, Lippincott, Williams &


Gill MS. Floppy Kid Syndrome. In Haskell (ed): Five-Minute Veterinary Consult: Ruminant, 1st edition. Philadelphia, Lippincott,


Gill MS. Penile Deviations. In Haskell (ed): Five-Minute Veterinary Consult: Ruminant, 1st edition. Philadelphia, Lippincott, Williams

& Wilkins, March, 2009.

Gill MS. Penile Hematoma. In Haskell (ed): Five-Minute Veterinary Consult: Ruminant, 1st edition. Philadelphia, Lippincott,


Gill MS. Preputial Prolapse. In Haskell (ed): Five-Minute Veterinary Consult: Ruminant, 1st edition. Philadelphia, Lippincott,


JOHNSON

Immunologic Disorders, Chapter 53. Consulting Editor, Large Animal Internal Medicine, ed. Smith B. St. Louis: Mosby Elsevier.

2009

Sellon DC, Hines MT, Johnson JR. Equine Immunodeficiency Diseases, in Large Animal Internal Medicine, ed. Smith B. St. Louis:

Mosby Elsevier. 2009, pp. 1665-1667

Johnson JR, Parish SM. Diseases caused by allogeneic incompatibilities in Large Animal Internal Medicine, ed. Smith B. St. Louis:

Mosby Elsevier. 2009, pp. 1682-1691.

LYLE

Lyle SK. Sexual development disorders. In: Tilley LP, Smith FWK (eds.) The 5-Minute Veterinary Consult: Canine and Feline, 4th

ed, Ames, IA, Blackwell Publishing, 2008.

Lyle SK. Vaginal malformations and acquired lesions. In: Tilley LP, Smith FWK (eds.), The 5-Minute Veterinary Consult: Canine

14

and Feline, 4th ed, Ames, IA, Blackwell Publishing, 2008.

Lyle SK. Infectious problems in the last trimester of pregnancy. In: Samper JC (ed.). Equine Breeding Management, 2nd ed.

Saunders Elsevier, Philadelphia PA; 2009; 249-254.

MCCONNICO

McConnico RS. Hubert J, Use of Slings in Equine Patients. In Current Therapy in Equine Medicine. 6th Ed., Robinson NE, ed. WB

Saunders Co., Philadelphia, PA., pp. 918-921, 2008.

McConnico RS. Acute Equine Colitis. In Current Therapy in Equine Medicine. 6th Ed., Robinson NE, ed WB Saunders Co,

Philadelphia, PA., pp 418-422, 2008.

McConnico RS. Emergency Equine Sheltering In: Veterinary Disaster Response, Eds. Wingfield WE, Palmer, SB. Blackwell

Publishing, Ames, IA, Ch 1.2 pp. 325-334. 2009.

McConnico RS, Equine review chapters (13) (restraint, behavior, nutrition, preventive medicine, infectious diseases, endocrine

disorders, hematology/ cardiology, gastrointestinal diseases, neurology, urology/nephrology, theriogenology, musculoskeletal

disorders, dentistry) In: Saunders Comprehensive Review for the NAVLE Board Review and Exam Practice Package, Schenck P,

Ed., Saunders, Philadelphia, PA October 2009.

MITCHELL

Mitchell CF, Leise B. Equine Surgery. In: Schenck PA (ed), Comprehensive Review for the NAVLE, 1st ed, St. Louis, Missouri,

Saunders/Elsevier, 2010, pp 495-538.

Mitchell CF, Moore RM, Gill MS. Large Animal Surgical Nursing. In: McCurnin DM, Bassert JM (eds), Clinical Textbook for

Veterinary Technicians, 7th ed, St. Louis, Missouri, Elsevier/Saunders, 2008).

PACCAMONTI

Pinto CRF, Paccamonti DL. Mare Reproductive Pathology. In: Disorders of the Reproductive Tract, Ch 18, Frazer G, ed., In:

Equine Internal Medicine, 3rd ed., Reed SM, Bayly WM, Sellon DC, eds. Saunders Elsevier, St. Louis, 2010, p 1016-1024.

Pinto CRF, Eilts BE, Paccamonti DL. Animal Reproduction. In: Clinical Textbook for Veterinary Technicians,7th ed. McCurnin DM

and Bassert JM, eds. Saunders Elsevier, St. Louis, 2010, p. 370--399.

Paccamonti D, Pycock J. Infertility and subfertility in the mare. In: Veterinary Reproduction and Obstetrics, 9th ed. Noakes DE,

Parkinson TJ and England GCW, eds. Saunders Elsevier, Edinburgh, 2009, p 582-631.

Paccamonti D, Parturition and Evaluation of the Placenta. In: Equine Breeding Management and Artificial Insemination, 2nd ed.


15


Samper JC, ed., Saunders Elsevier, St. Louis, 2009, p 255-259.

Refereed Publications

ANDREWS

Andrews FM, Buchanan BR, Elliott SB, Al Jassim RAM, McGowen CM, and Saxton AM. In vitro effects of hydrochloric and lactic

acids on bioelectric properties of equine gastric squamous mucosa. Equine Veterinary Journal 40(3):301-305, 2008.

Kelmer G, Doherty TJ, Elliott S, Saxton A, Fry MM, and Andrews FM. Evaluation of dimethyl sulphoxide effects on initial response

to endotoxin in the horse. Equine Veterinary Journal 40(4):358-363, 2008.

Witonsky SG, Ellison S, Yang J, Gogal RM, Lawler H, Suzuki Y, Sriranganathan N, Andrews F, Ward D, Lindsay DS. Horses

experimentally infected with Sarcocystis neurona develop altered immune response in vitro. Journal of Parasitology 94(5):1047-

1054, 2008.

Andrews FM, Reinemeyer CR, Longhoffer SL. Effects of top-dress formulations of suxibuzone and phenylbutazone on

development of gastric ulcers in horses. Veterinary Therapeutics 10(3):113-120, 2009.

Peretich AL, Abbott LL, Andrews FM, Dhar MS. Age-dependent regulation of sodium-potassium adenosinetriphosphatase and

sodium-hydrogen exchanger RNAs in equine nonglandular mucosa. American Journal of Veterinary Research 70(9):1124-1128;

2009.

BURBA

Lewis AJ, Sod GA, Burba DJ. Relationship of Fracture Fragment Thickness to Interfragmentary Compression Following Placement

of 6.5-mm Headless Tapered Variable Pitch and 5.5-mm AO Cortical Bone Screws for Fixation of a Simulated Lateral Condylar

Fracture in Equine Third Metacarpal Bones. Veterinary Surgery.

Lewis AJ, Sod GA, Burba DJ, Mitchell CF. Compression of condylar fractures of varying fragment thickness with acutrak plus vs

4.5-mm ao cortical screws in equine third metacarpus. Veterinary Surgery, 39(1), 78-82, 2010.

EADES

Eades SC, Fugler LA, Riggs L. Controlling the equine neutrophil: A generator of devastating tissue damage during equine

inflammatory disease. Vet J. 2008 Oct;178(1):3-4

Costa LR, Eades SC, Venugopal CS, Moore RM. Plasma and pulmonary fluid endothelin in horses with seasonal recurrent airway

obstruction. J Vet Int Med. 23(6):1239-46, 2010

EILTS

Len JA, Jenkins JA, Eilts BE, Paccamonti DL, Lyle SK, Hosgood G. Immediate and delayed (after cooling) effects of centrifugation

on equine sperm. Theriogenology. 2010 Jan 15;73(2):225-31.

GILL

Sod GA, Mitchell CF, Hubert JD, Martin GS, Gill MS. An in vitro biomechanical comparison of a 5.5 mm limited-contact dynamic

compression plate fixation with a 4.5 mm limited-contact dynamic compression plate fixation of osteotomized equine third

16

metacarpal bones. Veterinary Surgery. 37(3), 289-294, 2008.

Sod GA, Mitchell CF, Hubert JD, Martin GS, Gill MS. An in vitro biomechanical comparison of a locking compression plate fixation

with a limited-contact dynamic compression plate fixation of osteotomized equine third metacarpal bones. Veterinary Surgery.

37(3), 283-288, 2008.

Johnson SW, Gill MS, Grenier C, Taboada J. A descriptive analysis of personality and gender at the Louisiana State University

School of Veterinary Medicine. JVME. 35: 284-290, 2009.

Lewis AJ, Sod GA, Gill MS, Mitchell CF. Bovine distal interphalangeal joint arthrodesis using the acutrak plus screw: a report of

seven cases. Veterinary Surgery. 38(5), 659-663, 2009.

Sod GA, Riggs, LM, Mitchell CF, Martin GS, Gill MS. In vitro cyclic fatigue comparison of a modified 5.5 mm locking compression

plate fixation with a 5.5 mm locking compression plate fixation of osteotomized equine third metacarpal bones. Veterinary Surgery,

39(7), 833-838, 2010.

Sod GA, Riggs, LM, Mitchell CF, Martin GS, Gill MS. An in vitro biomechanical comparison of a 5.5 mm locking compression plate

fixation with a 4.5 mm locking compression plate fixation of osteotomized equine third metacarpal bones. Veterinary Surgery,

39(5), 581-587, 2010.

JOHNSON

Vidal MA, Robinson SL, Lopez MJ, Paulsen DB, Borkhsenious O, Johnson JR, Moore RM, Gimble JM. Comparison of

chondrogenic potential in equine mesenchymal stromal cells derived from adipose tissue and bone marrow. Vet Surgery 37:713-

724, 2008.

Hanly BK, Stokes AM, Bell AM, Johnson JR, Keowen ML, Paulsen DB, Sod GA, Moore RM. Use of serial laminar tissue collection

via biopsy in conscious healthy horses. Am J Vet R 70:697-702, 2009.

LOPEZ

Lopez MJ, McIntosh KR, Spencer ND, Borneman JN, Horswell R, Anderson P, Yu G, Gaschen L, Gimble JM. Acceleration of

spinal fusion using syngeneic and allogeneic adult adipose derived stem cells in a rat model. J Orthop Res 27:366-373, 2009.

Schaefer SL, Lu Y, Seeherman H, Li XJ, Lopez MJ, Markel, MD. The effect of rhBMP-2 on tibial plateau fractures in a canine

model. J Orthop Res 27:466-471, 2009.

McIntosh K, Lopez MJ, Borneman J, Spencer N, Anderson P, Gimble JM. Immunogenicity of allogeneic adipose-derived stem cells

in a rat spinal fusion model. Tissue Eng Part A 15:2677-86, 2009.

Lopez MJ, Davis KM, Jeffrey-Borger SL, Markel MD, Rettenmund C. Inter-observer repeatability of measurements on computed

tomography images of lax canine hip joints from youth to maturity. Vet Surg 38:920-6, 2009.

Snow LA, White R, Gustafson S, Xie L, Hosgood G, Monroe WT, Casey JP, Lopez MJ. Ex vivo comparison of three surgical


17

techniques to stabilize canine cranial cruciate ligament deficient stifles. Vet Surg 39:195-207, 2010.

Xie L, Spender ND, Beadle RE, Gaschen L, Buchert MR, Lopez MJ. Effects of athletic conditioning on horses with degenerative

suspensory ligament desmitis: A preliminary report. Vet Journal e-pub 6/2010.

LYLE

Lyle, S. K., Paccamonti, D. L. (2010). High risk pregnancy in the mare - practical implications for the practitioner. Pferdeheilkunde,

26(1), 29-35.

Paccamonti, D. L., Lyle, S. K. (2010). A Clinical View of Mare Reproductive Physiology. Pferdeheilkunde, 26(1), 10-13.

Len, J. A., Jenkins, J. A., Eilts, B. E., Paccamonti, D. L., Lyle, S. K., Hosgood, G. (2010). Immediate and delayed (after cooling)

effects of centrifugation on equine sperm. Theriogenology, 73(2), 225-231.

MCCAULEY

McCauley CT. Large Animal Emergency Nursing. In McCurnin DM, Bassert JM (eds) Textbook for Veterinary Technicians 7th Ed

7th ed, Elsevier, 2008 - 2009

MCCONNICO

Briere CA, Hosgood G, Morgan TW, Hedlund CS, Hicks M, McConnico RS. Effects of carprofen on the integrity and barrier function

of canine colonic mucosa. J Am Vet Med Assoc. 1;232(3):398. 2008

Briere CA, Hosgood G, Morgan TW, Hedlund CS, Hicks M, McConnico RS. Effects of carprofen on the integrity and barrier function

of canine colonic mucosa. Am J Vet Res.;69(2):174-81 2008

McConnico RS, Morgan TW, Williams CC, Hubert JD, Moore RM. Pathophysiologic effects of phenylbutazone on the right dorsal

colon in horses. Am J Vet Res. 2008 Nov;69(11):1496-505, 2009.

McConnico RS, Lunn DP, Review of controversial issues (nomenclature, case definition, diagnostic testing, and management

practices) in equine herpes myelitis (EHM), Proceedings of the 56th Annual Convention of the American Association of Equine

Practitioners Baltimore, MD, Dec. 5-8, 2010

MITCHELL

Gudehus T, Mirza MH, Mitchell CF. A milliliter of prevention. Compendium Equine. 3(1), 55-56, 2008.

Sod GA, Mitchell CF, Hubert JD, Martin GS, Gill MS. An in vitro biomechanical comparison of a locking compression plate fixation

with a limited-contact dynamic compression plate fixation of osteotomized equine 3rd metacarpal bones. Veterinary Surgery. 37(3),

283-288, 2008.

Sod GA, Mitchell CF, Hubert JD, Martin GS, Gill MS. An in vitro biomechanical comparison of a 5.5mm limited-contact dynamic

compression plate fixation with a 4.5mm limited-contact dynamic compression plate fixation of osteotomized equine 3rd metacarpal


18

bones. Veterinary Surgery. 37(3), 289-299, 2008.

Lewis AJ, Sod GA, Mitchell CF. Distal interphalangeal joint arthrodesis using the acutrak plus screw in 7 cattle. Veterinary Surgery.

38(5), 659-663, 2009.

Sod GA, Riggs, LM, Mitchell CF, Hubert JD, Martin GS. An in vitro biomechanical comparison of equine proximal interphalangeal

joint arthrodesis techniques: axially positioned dynamic compression plate and two abaxial transarticular cortical screws inserted in

lag fashion versus three parallel cortical screws inserted in lag fashion. Veterinary Surgery. 39(1), 83-90, 2010.





39(7), 833-838, 2010.



39(5), 581-587, 2010.

PACCAMONTI

Paccamonti DL, Carnevale E. Early embryonic death in mares. Clinical Theriogenology. 2:99-110, 2010.

Paccamonti DL, Lyle SK. A clinical view of mare reproductive physiology. Pferdeheilkunde. 26:10-13, 2010.

Lyle SK, Paccamonti DL. High risk pregnancy in the mare – practical implications for the practitioner. Pferdeheilkunde. 26:29-35,

2010.

Immediate and delayed (after cooling) effects of centrifugation on equine sperm. Len, J.A., Jenkins, J.A., Eilts, B.E., Paccamonti,

D.L., Lyle, S.K., Hosgood, G. Theriogenology, 73:225-231, 2010.

Wirtu, G., Pope, C.E., Paccamonti, D.L., Godke, Robert. A., Dresser, B.L. Ultrasonographic-guided retrieval and in vitro maturation

of eland (Taurotragus oryx) and bongo (Tragelaphus eurycerus isaaci) antelope oocytes. Animal Reproduction Science 111:160-

172, 2009.

Parlevliet, J.M., Paccamonti, D.L., Barker, S.A. Cefquinome concentrations in endometrium after intrauterine treatment of cobactan

4.5%® in mares and inflammatory response of the endometrium to this treatment. Reprod Dom Anim 44:189-193, 2009

Wirtu G, Pope CE, MacLean RA, Godke RA, Paccamonti DL, Dresser BL. Reversal of motility loss in bongo antelope (Tragelaphus

eurycerus isaaci) spermatozoa contaminated with hyposmotic urine during electroejaculation. Animal Reproduction Science

103:392-397, 2008.


19

Paccamonti DL, Carnevale E, Pregnancy loss during the embryonic period. Pferdeheilkunde. 24(1):92-94, 2008.

SOD

Sod GA, Mitchell CF, Hubert JD, Martin GS, Gill. MS. An in vitro biomechanical comparison of a locking compression plate fixation

with a limited-contact dynamic compression plate fixation of osteotomized equine third metacarpal bones. Veterinary Surgery.

37(3), 283-288, 2008.

Sod GA, Mitchell CF, Hubert JD, Martin GS, Gill. MS. An in vitro biomechanical comparison of a 5.5 mm limited-contact dynamic

compression plate fixation with a 4.5 mm limited-contact dynamic compression plate fixation of osteotomized equine third

metacarpal bones. Veterinary Surgery. 37(3), 289-299, 2008.

Hanly BK, Stokes AM, Bell AM, Johnson JR, Keowen ML, Paulsen DB, Sod GA, Moore RM. Use of serial laminar tissue collection

via biopsy in conscious healthy American Journal of Veterinary Research. 70(6), 697-702, 2009.

Lewis AJ, Sod GA, Mitchell CF. Distal interphalangeal joint arthrodesis using the acutrak plus screw in 7 cattle. Veterinary Surgery.

38(5), 659-663, 2009.



39(7), 833-838, 2010.



39(5), 581-587, 2010.

Sod GA, Riggs, LM, Mitchell CF, Hubert JD, Martin GS. An in vitro biomechanical comparison of equine proximal interphalangeal

joint arthrodesis techniques: axially positioned dynamic compression plate and two abaxial transarticular cortical screws inserted in

lag fashion versus three parallel cortical screws inserted in lag fashion. Veterinary Surgery. 39(1), 83-90, 2010.



VENUGOPAL

Venugopal, C.S., Holmes, E. P., Polikepahad, S., Laborde, S., Kearney, M., Moore, R.M. Neurokinin receptors in recurrent airway

obstruction: A comparative study of affected and unaffected horses. Can. J. Vet., Res., 73(1): 25-33, 2009.

Costa, L.R., Eades, S.C., Venugopal, C.S. Moore, R.M. Plasma and pulmonary fluid endothelin in horses with seasonal recurrent

airway obstruction. J Vet Intl. Med., 23: 12139-1246, 2009.


20


Non Refereed Publications

ANDREWS

Andrews F, Denovo R, Reese R, Elliott S, Moyers T, Barthel D, Lyman M, Daniel G. The Evaluation of the wireless SmartPill™ for

measuring gastric emptying and GI transit in normal dogs. Journal of Veterinary Internal Medicine 2008; 751.

Buchanan B, Andrews F, Elliott S, Al Jassim R, McGowan C, Saxton A. In vitro effects of lactic acid on bioelectric properties of

equine nonglandular squamous mucosa. Journal of Veterinary Internal Medicine 2008; 815.

Reese R, Andrews F, Elliott S, Saxton A, McMullin RB. The effect of Seabuckthorn extract in the treatment and prevention of

gastric ulcers in horses. Journal of Veterinary Internal Medicine 2008; 815.

Elliott S, Reese R, Denovo R, Barthel D, Lyman M, Danien G, Andrews, F. Use of a wireless capsule, SmartPill™ to measure

gastrointestinal pH, pressure and transit time in a horse. Journal of Veterinary Internal Medicine 2008; 815-816.

Andrews F, McConnico R. Cause of concern: Evidence that therapeutic dosing of nonselective NSAIDs contributes to

gastrointestinal injury (Clinical Commentary). Equine Veterinary Education 21(12):663-664, 2009.

BURBA

Burba DJ. Can cribbing be halted? The Horse. November 2008.

CHAPMAN

Eades SC and Chapman AM. Foal diarrhea and Pneumonia. Louisiana Horse Magazine, Spring 2010.

Chapman AM. Lawsonia intracellularis: An emerging pathogen in weanling foals. Louisiana Horse Magazine, Fall 2009.

MCCAULEY

McCauley CT. Cryptorchid Castration and Scrotal Ablation in Horses. DVM Magazine 2009.

MCCONNICO

McConnico RS, Preparing an equine practice for a natural disaster, Commentary DVM Magazine, Veterinarynews360, Dec 1, 2008

McConnico RS, Beware Bute’s Adverse Effects, Researchers Recommend. The Horse.com, Article # 13073 November 12, 2008

McConnico RS, Microchipping, Appaloosa Journal. Feb 2008

McConnico RS. Our responsibility for disaster preparedness: Fighting the “It won’t ever happen to me” attitude. Newsletter for the

American Association of Human Animal Bond Veterinarians, Spring 2008

Andrews FA, McConnico RS, Clinical Commentary: Cause for concern: Evidence that therapeutic dosing of non-selective NSAIDs

contributes to gastrointestinal injury. Eq Vet Edu. 21(12) pp. 663-64, Dec 2009

21


McConnico RS, Blood tests may detect early NSAID damage, Equus 377, p 10. Feb 2009

McConnico RS, BUTE: Not the Aspirin You Thought it Was, www.grayson-jockeyclub.org/news, Summer 2009

McConnico RS, Disaster Preparedness for the Horse Owner: Horse TV Stories, March 11, 2009

McConnico RS, Sykes M, Bute: Not the Aspirin You Thought It Was The Horsemen’s Journal, http://www.horseracingofficials.com ,

June 22, 2009

McConnico RS, Merial: Equine - Know the Signs of an NSAID Overdose, Cattlenetwork.com, 7/30/09

McConnico RS, LSART rescues horse from flood, The Daily Reveille, LSUReveille.com, Nov. 17, 2009

McConnico RS LSART rescues horse from flood, The Horse.com Nov 23, 2009

Equine Herpes Virus-1: Minimizing Costs, Dispelling Myths (AAEP 2010); December 2010

McConnico RS, Beware Bute’s Adverse Effects, Researchers Recommend. TheHorse.com, November 12 2008, Article # 13073

PACCAMONTI

Paccamonti DL. Leipziger Blaue Hefte - Proc. 5th Leipziger Tierarztekongress. Understanding the mare’s estrous cycle. P 236-240.

Paccamonti D, Mare Research Update. Clinical Theriogenology, 1:335-336, 2009.

Paccamonti D. Reproductive physiology and cyclicity in the mare from a clinical viewpoint; Insemination strategies in the mare.

Proc. Ann Mtg Vet. Med. Soc. Denmark, Copenhagen, 2008

Paccamonti D. Multiple ovulation and twinning; Fecundacion y gestacion temprana de la yegua (< 60 dias) y Manejo de la yegua

gestante y gestaciones gemelares. Proc. Curso Diplomado en Reproducion, Guadalajara, 2008

Abstracts

ANDREWS

Andrews F, Denovo R, Reese R, Elliott S, Moyers T, Barthel D, Lyman M, Daniel G. The Evaluation of the wireless SmartPill™ for

measuring gastric emptying and GI transit in normal dogs. Journal of Veterinary Internal Medicine 2008; 751.

Buchanan B, Andrews F, Elliott S, Al Jassim R, McGowan C, Saxton A. In vitro effects of lactic acid on bioelectric properties of

equine nonglandular squamous mucosa. Journal of Veterinary Internal Medicine 2008; 815.

Reese R, Andrews F, Elliott S, Saxton A, McMullin RB. The effect of Seabuckthorn extract in the treatment and prevention of

gastric ulcers in horses. Journal of Veterinary Internal Medicine 2008; 815.

22


Elliott S, Reese R, Denovo R, Barthel D, Lyman M, Danien G, Andrews, F. Use of a wireless capsule, SmartPill™ to measure

gastrointestinal pH, pressure and transit time in a horse. Journal of Veterinary Internal Medicine 2008; 815-816.

Reese RE, Andrews FM, Elliott SB, Saxton AM. Effect of volatile fatty acid mixes on equine nonglandular mucosa bioelectric

properties: Pathogenesis of gastric ulcers in horses. Journal of Veterinary Internal Medicine 2009; 700.

Andrews F, A Peretich, RE Reese, L Abbott, M Dhar. Role of cellular sodium transport in nonglandular equine gastric ulcer

disease. Journal of Veterinary Internal Medicine 2010; 24:792 (Abstract #399)

BEADLE

Mitchell CF, Beadle RE. Deep digital flexor tenotomy as a treatment for horses with chronic laminitis. Poster presentation at the

2nd AAEP Foundation Equine Laminitis Research Workshop, West Palm Beach, Florida. November 8-10, 2009.

Venugopal CS, Beadle RE, Holmes EP et al. Induction of insulin resistance in equine digital vessel rings [abstract]. J Vet Intern

Med 2010;24(3):790.

CHAPMAN

Buchert M, Gebhart C, Eades S, Johnson J, Chapman A. Identification of exposure to and shedding of Lawsonia intracellularis

in Louisiana horses using serology and real time PCR. Phi Zeta Research Day, LSU SVM, Baton Rouge, LA, September 2010.

(Poster Presentation).

Chapman AM. Identification of exposure to and shedding of Lawsonia intracellularis in Louisiana horses using serology and real

time PCR. First Annual Lawsonia intracellularis workshop. Haygard Medical Institute, Lexington, KY, June 25, 2009.

Johnson JR, Eades SC, Chapman A, Hosgood G. Implementing a geographical information system (GIS) to investigate nosocomial

Salmonella infections. 2nd International Symposium on Geospatial Health, New Orleans, LA, December 2008.

Johnson JR, Eades SC, Chapman AM, Truman C, Parker J. Temporospatial tracking of Salmonella infections in hospitalized

equine patients in a veterinary teaching hospital. 3rd Intl Symp on Geospatial Health, Veitri Sul Mare, Italy, September 2009.

Truman C, Johnson JR, Eades SC, Chapman AM, Parker J. Salmonellosis in LSU Equine Hospital 8/31/2000 to 5/19/2009. Phi

Zeta Research Emphasis Day, September 23, 2009, Baton Rouge, LA.

Thompson JA; Eades SC, Chapman AM, Barker SA; Paulsen DB; McConnico RS, Assessment of Cardiac Function, Skeletal

Muscle Morphology, and Muscle Enzymes in Horses on Long-Term Clenbuterol Therapy, Proceedings of the ACVIM Annual

Forum, Montreal, Quebec, Canada, June 3-6, 2009

EADES

Fugler LA, Eades SC. Clinical and Matrix Metalloproteinase Inhibitory Effects of Pentoxifylline on carbohydrate overload laminitis:

preliminary results. J Equine Vet Sci. 30:106, 2010.

23


EILTS

Len JA, Jenkins JA, Eilts B.E, Paccamonti DL, Lyle SK, Hosgood G. Centrifugation has minimal effects on motility,viability, and

acrosome integrity of equine sperm. Theriogenology 70: 582-583, 2008.

Jill A. Jenkins, Bruce E. Eilts, and Rassa O. Draugelis-Dale. Tools and Techniques Flow Cytometry: An Essential Tool in Biomarker

Research in Briggs, K.M., ed., 2010, Proceedings of the U.S. Geological Survey Interdisciplinary Microbiology Workshop, Estes

Park, Colorado, October 15–17, 2008: U.S. Geological Survey Scientific Investigations p 35.

Lyle, SK, Gentry, LR, Horohov, DW, Johnson, JR, Eilts, BE, Godke, RA, Paccamonti, DL. Preliminary evidence of fetal

hypothalamic-pituitary-adrenal axis activation in an experimental model of infective preterm delivery in the mare. Clinical

Theriogenology. Volume 1 Number 1. 2009. P 238.

Eilts BE, Pennington PM, Vermeulen MAE, Pope CE, Lyle SK, Len JA, Godke RA. Comparing Deslorelin Implants or Oral

Diethylstilbestrol to Induce Estrus in the Bitch. Clinical Theriogenology (2010):2 p 380.

Vermeulen MAE, Eilts BE, Hosgood G, Rademacher N, Pennington PM, Lyle SK, Len JA, Godke RA, Pope CE, Parlevliet JM.

Ovarian Color-Doppler Ultrasonography to Predict Ovulation in the Bitch. Clinical Theriogenology (2010):2 p 356.

Len JA, Vermeulen MAE, Eilts BE, Lyle SK. Low Dose Prostaglandin F2α for Luteal Regression in the Bitch. Clinical

Theriogenology (2010):2 p 362.

Beehan DP, Lyle SK, Len JA, Staempfli SA, Eilts BE. Investigation of preovulatory serum progesterone increase after hCG

treatment in mares. Animal Reproduction Science 121S (2010) S49–S50.

Causey RC, Calderwood Mays MB, Anderson KJ, Lyle SK, Paccamonti DL, Eilts BE, LeBlanc MM. Alteration of the uterine

epithelium in chronically infertile mares. Animal Reproduction Science 121S (2010) S92–S93.

Lyle SK, Hague M, Lopez MJ, Beehan DP, Staempfli SA, Len JA, Eilts BE, Paccamonti DL. In vitro production of cortisol by equine

fetal adrenal cells in response to ACTH and IL-1. Animal Reproduction Science 121S (2010) S322–S323.

GILL


fixation with a 4.5 mm locking compression plate fixation of ostectomized equine third metacarpal bones. Veterinary Orthopedic

Society 36th Annual Conference, Steamboat Springs, CO, February, 2009.

Gudehus TH, Sod GA, Mitchell CF, McCauley CT, Gill MS. An in vitro analysis of temperatures and insertion torques of HA coated

and uncoated 5.5 mm AO cortical screws. Veterinary Orthopedic Society 36th Annual Conference, Steamboat Springs, CO,

February, 2009.

JOHNSON

Schur D, Rademacher N, McCauley C, Lopez M, Spencer N, Vidal M, Burba D, Johnson J, , Gaschen L,. Ultrasound assessment

protocol and preliminary findings of equine tendon injury before and after stem cell therapy. Phi Zeta Day, LSU SVM, Baton Rouge,

LA, September 2008.

24


Vidal MA, Robinson SO, Lopez MJ, Paulsen DB, Johnson JR, Moore RM, Gimble JM. Comparison of chondrogenic potential in

mesenchymal stromal cells derived from adipose tissue and bone marrow. Vet Orthopedic Society, 2008.

Johnson JR, Eades SC, Chapman A, Hosgood G. Implementing a geographical information system (GIS) to investigate nosocomial

Salmonella infections. 2nd International Symposium on Geospatial Health, New Orleans, LA, December 2008.

Johnson JR, Eades SC, Chapman AM, Truman C, Parker J. Temporospatial tracking of Salmonella infections in hospitalized

equine patients in a veterinary teaching hospital. 3rd Intl Symp on Geospatial Health, Veitri Sul Mare, Italy, September 2009.

Truman C, Johnson JR, Eades SC, Chapman AM, Parker J. Salmonellosis in LSU Equine Hospital 8/31/2000 to 5/19/2009. Phi

Zeta Research Emphasis Day, September 23, 2009, Baton Rouge, LA.

EHSP Seminar, Implementing a GIS to study nosocomial salmonella in a veterinary teaching hospital. September 2009.

Chapman AM, Eades SC, Johnson JR, Gebhardt C. Identification of exposure to and shedding of Lawsonia intracellularis in

Louisiana horses using serology and real-time PCR.

Lyle SK, Gentry LR, Horohov DW, Johnson JR, Eilts Be, Godkey RA, Paccamonti DL. Preliminary evidence of fetal hypothalamic-

ituitary-adrenal axis activation in an experimental model of infective preterm delivery in a mare. Therio/ACT Annual Conference,

August 20009, Albuquerque, New Mexico.

LOPEZ

Xie L, Robinson S, Spencer N, Buchert M, Gaschen L, Lopez MJ. Effects of athletic conditioning on equine degenerative

suspensory ligament desmitis. American Association of Equine Practitioners, 2009.

Huff N, Spencer N, Gimble J, Bagby G, Nelson S, Lopez MJ. Impaired multipotential capacity of adult stem cells in a rat chronic

alcohol abuse model. Proceedings American College of Veterinary Surgeons Annual Symposium, 2009.

Lopez MJ, Spencer ND, Robinson S, Burba D, Miska T. Effects of monopolar radiofrequency energy and diode laser energy on

equine distal tarsal joint articular cartilage. Proceedings American College of Veterinary Surgeons Annual Symposium, 2009.

Lopez MJ, Spencer ND, Robinson S, Holliday J. Osteoprotegerin and receptor activator of nuclear factor kappa beta ligand

expression in normal and osteoarthritic canine hip joint articular cartilage. Proceedings American College of Veterinary Surgeons

Annual Symposium, 2009.

Lopez M. Effect of chronic alcohol consumption on adult mesenchymal stem cells. Proceedings Research Society on Alcoholism

Scientific Meeting, 2009.

Lopez MJ, Huff NK, Spencer ND, Nelson S, Bagby GJ, Gimble JM. Alterations in adult mesenchymal stromal cell growth,

expansion, and pluripotential associated with chronic alcohol consumption. Alcoholism 33:32A, 2009.

Ayer K, Patterson D, Lopez MJ, Murphy MC, Measuring skeletal kinematics with accelerometers on the skin surface, Proceedings

American Society of Mechanical Engineers, 2009.

25


Lopez MJ, Spencer N, Robinson S. Osteoprotegerin and receptor activator of nuclear factor kappa beta ligand expression in

normal and osteoarthritic hip joint articular cartilage in a canine model. Proceedings Orthopaedic Research Society E2205, 2009.

Spencer N, Chun R, Vidal MA, Gimble JM, Lopez MJ. In vitro comparison of adult canine bone marrow- and adipose derived

stromal cell growth characteristics, Proceedings 44th Annual Symposium American College of Veterinary Surgeons, 2010.

Lopez MJ. Breaking news on adult canine stem cells. Proceedings American Veterinary Medical Association Annual Convention,

2010.

Bragulla H, Foster AL, Lopez MJ. Soft and hard keratins in the hooves of equine fetuses and adult horses. XXVIIIth Congress of

the European Association of Veterinary Anatomists, 2010.

Lopez MJ, Huff N, Spencer J, Bagby G, Nelson S, Gimble J. Chronic Alcoholism Reduces Adult Stem Cell Function. Proceedings

56th Annual Meeting Orthopaedic Research Society, 2010.

LYLE

Bradecamp EA, Snyder S, Rocha A, Lyle SK, Len J, Eilts BE, Godke RA, Paccamonti DL. Can time of day of ovulation be

influenced by hCG? . Pferdeheilkunde 24:112, 2008. (abstr)

Mocklin CM, Lyle SK, Eilts BE, Wouters EGH, Gentry LR, Godke RA, Paccamonti DL. Is the individual variation in response to

post-ovulatory cloprostenol in mares due in part to timing of initiation of treatment in relation to ovulation? Pferdeheilkunde 24:112,

2008. (abstr)

Lyle SK, Gentry LR, Horohov DW, Johnson JR, Eilts BE, Godke RA, Paccamonti DL. Preliminary evidence of fetal hypothalamic-

pituitary-adrenal axis activation in an experimental model of infective preterm delivery in the mare. Clinical Theriogenology. Volume

1 Number 1. 2009. P 238.

Causey RC, Mays MM, Anderson KJ, Lyle SK, Paccamonti DL, Eilts BE, LeBlanc MM. (2010). Alteration of the uterine epithelium

in chronically infertile mares Anim Repro Sci 121 Supp 1-2: 92-93).

Staempfli SA, Clavier S, Beehan DP, Lyle SK, Thompson DL, Burns PJ, McKinnon AO. (2010). Effect of a single injection of long-

acting progesterone on first ovulation in early and late transitional mares. Anim Repro Sci 121 Supp 1-2: 26-27.

Lyle SK, Hague MJ, Lopez MJ, Beehan DP, Staempfli S, Eilts BE, Paccamonti DL. (2010). In vitro production of cortisol by equine

fetal adrenal cells in response to ACTH and IL-1α. Anim Repro Sci 121 Supp 1-2: 322-323.

Beehan DP, Lyle SK, Len JA, Staempfli SA, Eilts BE. (2010). Investigation of preovulatory progesterone increase after hCG

treatment in mares. Anim Repro Sci 121 Supp 1-2: 49-50.

Lyle, S. K., Gentry, L. R., Johnson, J. R., Eilts, B. E., Godke, R. A., Paccamonti, D. L. (2010). Maturation of the equine fetal

hypothalamic pituitary axis in response to in utero inflammation. Pferdeheilkunde 26(1): 47.

26


Gentry, L. R., Gentry, G., Bonura, A., Staempfli, S. A., Len, J. A., Lyle, S. K., Burns, P., Paccamonti, D. L. (2010). Suppression of

follicular activity in mares using high dose estrogen. Pferdeheilkunde 26(1):28.

MCCONNICO

Hayden M, McConnico RS, Barker S, Comparative Metabolism of Levamisole in Horses and Cattle: Formation of the Stimulant

Drug Aminorex, Merial Summer Veterinary Student Honors Research Symposium, Michigan State University, East Lansing, MI,

2008

McConnico RS, Eller W, The Role of the Veterinarian in Community Disaster Planning and Response, U.S. Dept of Health &

Human Services National Disaster Medical Systems Training Summit, Nashville, TN May 15-18, 2008

Rigney H, Poirrier RA, McConnico RS, Implementation of the PETS Act with USDA-APHIS Officials and Louisiana Dept. of

Agriculture & Forestry, U.S. Dept of Health & Human Services National Disaster Medical Systems Training Summit, Nashville, TN

May 15-18, 2008

McConnico RS, Roy AF, Eades SC, Andrews F, Comparison of PCR Testing for Management of an Outbreak of EHVI at a

Racetrack, Proceedings of the ACVIM Annual Forum, Montreal, Quebec, Canada, June 3-6, 2009

Thompson JA; Eades SC, Chapman AM, Barker SA; Paulsen DB; McConnico RS, Assessment of Cardiac Function, Skeletal

Muscle Morphology, and Muscle Enzymes in Horses on Long-Term Clenbuterol Therapy, Proceedings of the ACVIM Annual

Forum, Montreal, Quebec, Canada, June 3-6, 2009

McConnico RS, The Role of the Veterinarian in Disaster Animal Response, Proceedings of the USDA Foreign Animal and

Emerging Diseases (FAED) Symposium, University of Tennessee, Knoxville, TN, August 3-7, 2009

Khodadad J, McConnico RS, Examining the pharmacokinetics and safety of firocoxib, a COX-1 sparing NSAID in foals Merial

Summer Scholars Program, National Symposium, NCSU-CVM, Raleigh, NC, Aug 2009

McConnico RS, The Role of the Veterinarian in Disaster Animal Response, Proceedings of Purina’s Equine Veterinary Conference,

Purina Mills, Inc., St. Louis, MO, October 2-4, 2009

Sousa D, Poirrier R, Adcock RA, McConnico RS, The impact of animals on evacuation management, Proceedings of the National

Evacuation Conference, New Orleans, LA, Feb 3-5, 2010

McConnico RS, Navarre CB, Disaster Preparedness: All Hazards/All Animals.

Proceedings of the Southwest Veterinary Symposium, Fort Worth, Texas, September 23 – 26, 2010

McConnico RS, Equine Disaster Preparedness & Mitigation Proceedings of the Southwest Veterinary Symposium, Fort Worth,

Texas, September 23 – 26, 2010

Fernandez RR, Koch C, McConnico RS, Effects of Buscopan and flunixin on permeability and secretory capacity of equine right

dorsal colonic mucosa, Merial Summer Scholars Program, Merial Summer Scholars Program, National Symposium, Athens, GA,

Aug, 2010

27


MITCHELL

Sod GA, Mitchell CF, Hubert JD, Martin GS, Gill MS. Why do orthopedic implants fail in equine long bone fracture patients? – Part

1. Veterinary Orthopedic Society 35th Annual Conference, Big Sky, MT, March 2008.


2. Veterinary Orthopedic Society 35th Annual Conference, Big Sky, MT, March 2008.

Lewis AJ, Sod GA, Burba DJ, Mitchell CF. Compression of fracture fragments of varying thickness with acutrak plus or 4.5mm ao

cortical screws in equine 3rd metacarpal condylar fractures. Veterinary Orthopedic Society 35th Annual Conference, Big Sky, MT,

March 2008.

Lewis AJ, Sod GA, Mitchell CF. Bovine distal interphalangeal joint arthrodesis using the acutrak plus screw. Veterinary Orthopedic

Society 35th Annual Conference, Big Sky, MT, March 2008.

Mitchell CF, Beadle RE. Deep digital flexor tenotomy as a treatment for horses with chronic laminitis. Poster presentation at the

2nd AAEP Foundation Equine Laminitis Research Workshop, West Palm Beach, Florida. November 8-10, 2009.

Gudehus TH, Sod, GA, Mitchell CF, MCCauley CT. An in vitro analysis of temperatures and insertion torques of HA coated and

uncoated 5.5 mm AO cortical screws. Proceedings of the 44th Annual American College of Veterinary Surgeons Symposium,

Washington, D.C., October 2009.


fixation with a 4.5 mm locking compression plate fixation of osteotomized equine third metacarpal bones. Veterinary Orthopedic

Society 36th Annual Conference, Steamboat Springs, CO, February 2009.

Gudehus TH, Sod, GA, Mitchell CF, McCauley CT, Gill MS. An in vitro analysis of temperatures and insertion torques of HA coated


February 2009.

Sod GA, Riggs, LM, Mitchell CF, Martin GS. An in vitro biomechanical comparison of equine proximal interphalangeal joint

arthrodesis techniques: a new axial positioned LCP versus an axial positioned DCP each with two abaxial transarticular cortical

screws inserted in lag fashion. Veterinary Orthopedic Society 37th Annual Conference, Breckenridge, CO, February 2010.

PACCAMONTI

Causey RC, Calderwood Mays MB, Anderson KJ, Lyle SK, Paccamonti DL, Eilts BE, LeBlanc MM. Alteration of the uterine

epithelium in chronically infertile mares. Anim Reprod Sci 121S:S92-S93, 2010.

Lyle SK, Hague M, Lopez MJ, Beehan DP, Staempfli S, Len J, Eilts BE, Paccamonti DL. In vitro production of cortisol by equine

fetal adrenal cells in response to ACTH and IL-1β. Anim Reprod Sci 121S:S322-S323, 2010.

Gentry L, Gentry G, Bonura A, Staempfli S, Len J, Lyle S. Burns P, Paccamonti D. Suppression of follicular activity in mares using

high dose estrogen. Pferdeheilkunde.26:28, 2010.

28


Lyle SK, Gentry LR, Horohov DW, Johnson JR, Eilts BE, Godke RA, Paccamonti DL. Maturation of the fetal hypothalamic pituitary

axis in response to in utero inflammation. Pferdeheilkunde. 26:47, 2010.

Lyle SK, Gentry LR, Horohov DW, Johnson JR, Eilts BE, Godke RA, Paccamonti DL. Preliminary evidence of fetal hypothalamic-

pituitary-adrenal axis activation in an experimental model of infective preterm delivery in the mare. Clinical Theriogenology. 1:238,

2009.

Lyle SK, Gentry LR, Horohov DW, Johnson JR, Eilts BE, Godke RA, Paccamonti DL. Preliminary evidence of fetal cortisol

production in response to inflammation in a model of equine placentitis. Proc Am Assoc Eq Pract, 55: 264-265, 2009.

Len JA, Jenkins JA, Eilts BE, Paccamonti DL, Lyle SK, Hosgood G. Centrifugation has minimal effect on motility, viability, and

acrosome integrity of equine sperm. Theriogenology 70:582-583, 2008.

Mocklin CM, Lyle SK, Eilts BE, Wouters EGH, Gentry LR, Godke RA, Paccamonti DL. Is the individual variation in response to

post-ovulatory cloprostenol in mares due in part to timing of initiation of treatment in relation to ovulation? Pferdeheilkunde, 24:112,

2008.

Bradecamp EA, Snyder A, Rocha A, Lyle SK, Len J, Eilts BE, Godke RA, Paccamonti DL. Can time of day of ovulation be

influenced by hCG? Pferdeheilkunde 24:112, 2008.

Saenz JR, Guerrero C, Paccamonti D, Eilts B, Bondioli K, Godke RA. Processing of postmortem bovine epididymal sperm after

cooling the testes for 24 Hours. Proc Intl Emb Transf Soci, 2008.

SOD

Sod GA, Mitchell CF, Hubert JD, Martin GS, Gill MS. Why do orthopedic implants fail in equine long bone fracture patients? - Part I.

Veterinary Orthopedic Society 35th Annual Conference, Big Sky, MT, March 2008.


II. Veterinary Orthopedic Society 35th Annual Conference, Big Sky, MT, March 2008.

Lewis AJ, Sod GA, Burba DJ, Mitchell CF. Compression of fracture fragments of varying thickness with acutrak plus or 4.5mm ao

cortical screws in equine third metacarpal condylar fractures. Veterinary Orthopedic Society 35th Annual Conference, Big Sky, MT,

March 2008.

Lewis AJ, Sod GA, Mitchell CF. Bovine distal interphalangeal joint arthrodesis using the acutrak plus screw. Veterinary Orthopedic

Society 35th Annual Conference, Big Sky, MT, March 2008.

Durham ME, Sod GA, Lewis AJ. An in vitro analysis of insertion characteristics of hydroxyapatite-coated and uncoated positive

profile transfixation pins using a stepped drill bit in the third metacarpal bone of horses. Proceedings of the 44th Annual American

College of Veterinary Surgeons Symposium, Washington, D.C., October 2009.

Durham ME, Sod GA, Riggs LM, Lewis AJ. Laparoscopic comparison of adhesion formation associated with placement of a

macroporous condensed polytetrafluoroethylene mesh vs. polypropylene mesh in the equine abdomen. Proceedings of the 44th

29


Annual American College of Veterinary Surgeons Symposium, Washington, D.C., October 2009.


fixation with a 4.5 mm locking compression plate fixation of osteotomized equine third metacarpal bones. Veterinary Orthopedic

Society 36th Annual Conference, Steamboat Springs, CO, February 2009.

Gudehus TH, Sod, GA, Mitchell CF, McCauley CT, Gill MS. An in vitro analysis of temperatures and insertion torques of HA coated


February 2009.

Sod GA, Riggs, LM, Mitchell CF, Martin GS. An in vitro biomechanical comparison of equine proximal interphalangeal joint

arthrodesis techniques: a new axial positioned lcp versus an axial positioned dcp each with two abaxial transarticular cortical

screws inserted in lag fashion. Veterinary Orthopedic Society 37th Annual Conference, Breckenridge, CO, February 2010.

Sod GA and Vidal MA. An assessment of locking and AO screw position to increase cyclic fatigue during axial compression of an

oblique mid-diaphyseal equine third metacarpal fracture model. 12th Biennial Conference of the International Society for Fracture

Repair, London, England, September 2010.

VENUGOPAL

Venugopal CS, Mariappan N, Holmes EP, Francis J, Eades SC. Bronchial oxidative stress in equine recurrent airway obstruction.

This study was presented at the Experimental Biology meeting in New Orleans, April 21, 2009.

Venugopal CS, Mariappan N, Koch C, Holmes EP. Evidence for interaction between endothelin receptors in equine airways. This

study was presented at the Experimental Biology meeting in New Orleans, April 21, 2009.

Venugopal CS. Role of endothelin-1 in equine recurrent airway obstruction. This was presented at the 4th World equine airways

symposium in August 7th 2009 (Bern, Switzerland).

30

Dr. Frank Andrews:

Validation of the use of the SmartPill® Capsule for measurement of transit time, pH, temperature and luminal pressure in the

gastrointestinal tract of horses and dogs. SmartPill® Corporation, Buffalo, NY. 2007-2010. $21,000 (Transferred from UT).

An evaluation of the clinical efficacy of pergolide mesylate tablets for the control of clinical signs associated with pituitary pars

intermedia dysfunction (Equine Cushing’s Disease) in horses. Elanco Animal Health and Boehringer Ingelheim Vetmedica, Inc.

2008-2010. $214,358.

Extended use of pergolide mesylate in horses. Boehringer Ingelheim Vetmedica, Inc. 2009-2010. Up to $9,000.

Dr. Ann Chapman:

Chapman AM, Eades SC, Johnson JR. Identification of exposure to and shedding of Lawsonia intracellularis in Louisiana horses

using serology and real time PCR, EHSP Corp Fund, $12,000

Chapman AM. Identification of fecal shedding of Salmonella on a Virginia Thoroughbred breeding farm using real time PCR. VCS

Faculty start-up funds, $2,000

Dr. Shafiqul Chowdhury:

ELI LILLY. Improved Genetically Engineered Vaccines Against Bovine Herpesvirus Type 1 (BHV-1) and Equine Herpesvirus (EHV-

1) Type 1 Viruses. Total award $315,000 (12-20-2010- 12-19- 2012).

Dr. Susan Eades:

Eades SC, Fugler LA. Treatment of equine laminitis with Doxycycline. Grayson Foundation Special Barbaro Fund. $58,585.

2008-2010.

Eades SC, Fugler LA. The effects of oral administration of doxycycline on matrix metalloproteinase inhibition and experimentally-

induced laminitis. $25,000. Equine Health Studies Program, Louisiana State University School of Veterinary Medicine.

Fugler LA, Eades SC. Effects of LPS on matrix metalloproteinase release and biomechanical testing in laminar explants in vitro.

$6,000. Equine Health Studies Program, Louisiana State University School of Veterinary Medicine

McConnico R, Thompson J, Eades S, Paulsen D. Effects of clenbuterol on skeletal muscle composition, cardiac function and

biochemical markers of muscle injury in exercising horses. $12,500. Equine Health Studies Program, Louisiana State University

School of Veterinary Medicine

Venugopal C, Francis J, Eades S. Role of oxidative stress and endothelin in recurrent airway obstruction (RAO) in horses. $12,500.

Equine Health Studies Program, Louisiana State University School of Veterinary Medicine

Venugopal CS, Eades SC. Identification of a marker for equine summer pasture-associated obstructive pulmonary disease

(SPAOPD). Equine Health Studies Program. 2008-2009, $12,500.

Chapman A. Identification of Lawsonia Intracellularis in horses in Louisiana. LSU EHSP. $12,500.

2008-2010 GRANTS

31

2008-2010 GRANTS

Venugopal C. In vitro investigation of insulin resistance on equine digital vessels. LSU EHSP. $12,500.

Eades S, Andrews F. Validating a model to measure digital arterial pressure in the horse. ELANCO Animal Health. October 2010 to

December 2010. $25,312.

Eades S, Andrews F. The Pharmacokinetics and Vasoactive Properties of LSN2798286 (Amino-Tadalafil) in Mature Horses

ELANCO Animal Health. November 2010 to November 2011. $44,979.

Venugopal C, Francis J, Beadle R, Eades S. Role of oxidative stress and endothelin in recurrent airway obstruction (RAO) in

horses. EHSP 2010. $12,000.

Dr. Bruce Eilts:

A Canine Model For Nonsurgical Embryo Recovery, Embryo Cryopreservation, Embryo Culture and Nonsurgical Embryo Transfer

In Endangered Canids. $97,036

In vivo fertilization and culture of ungulate embryos in the bovine oviduct. ACRES. $50,000.

Dr. Jill Johnson:

Implementing a geographical information system (GIS) to investigate nosocomial Salmonella infections. Johnson JR, Eades SC,

Chapman A, Hosgood G. EHSP, $3,640.

Johnson JR. Capturing Salmonella from Biofilm in Water Sources, Pall Life Sciences provided water filters for screening water

sources in equine hospital for Salmonella organisms.

Chapman AM, Eades SC, Johnson JR. Detection of Lawsonia intracellularis in horses in Louisiana using serology and real-time

PCR. Equine Health Studies Program, $12,000.

Dr. Jose Len:

Len J, Beehan D. Effects on equine sperm cells of centrifugation with or without cushion solution. Funded by a $3,000 grant from

the EHSP.

Dr. Mandi Lopez:

Comparison of Two Soft Tissue Fixation Devices for Anterior Ligament Cruciate Reconstruction in a Canine Model. Tesa Medical,

Inc., $71,013. (4/2010 – 10/2010).

Lopez MJ, Gimble J. Equine bone regeneration with adult stem cells Grayson Jockey Club Foundation, $157,830. (4/2010

– 3/2012).

Lopez MJ. Travel Grant for Emerging Faculty. LA Board of Regents, $1,200. (8/2010).

Lopez MJ. Optimization and Characterization of Equine Adult Stem Cells for Bone Regeneration. LSU SVM USDA, $7,500.

(09/2009 – 08/2010).

32

Lopez MJ, Lin X, Zanetti A. Optimization and characterization of equine adult stem cells for bone regeneration. LSU Equine Health

Studies Program, $7,500. (7/2009-9/2010).

Lopez MJ, LRN Kaidapuram. Generation of a computer model of equine motion for therapeutic riding. LSU Equine Health Studies

Program, $6,000. (7/2009-6/2010)

Lopez MJ, Xie L. Comparison and quantification of equine adipose and marrow derived adult stem cell adhesion, viability,

proliferation, and induction behavior on three bioabsorbable scaffolds. LSU Equine Health Studies Program, $12,000.

(7/2009-6/20010)

Lopez MJ, Zanetti A. Osteogenic differentiation of equine adult stem cells (EASCs) in biodegradable scaffolds: a rat model for

equine bone reconstruction. LSU Equine Health Studies Program, $12,000. (7/2009-6/20010)

Lopez MJ. Travel Grant for Emerging Faculty. LA Board of Regents, $1,200. (6/2009)

Gao A., Lopez MJ. Post-doctoral training Fellowship People’s Republic of China $40,000. (6/22/2009-6/21/2010)

Lopez MJ, Gimble JM. Optimization of Adult Adipose Stem Cells for Bone Healing . LA EPSCoR (PFUND), $10,000.

(4/1/09-3/31/10)

Bagby G, Molina P, Shellito J (New Orleans Health Sciences Center, Lopez Core Training Faculty Member). T32: Biomedical

Alcohol Research Training Program (#2T32AA007577-11). NIH-NIAAA $1,952,500. (9/1/09-8/31/14)

Nelson S (PI, New Orleans Health Sciences Center. Lopez collaborator). Alcohol, HIV and Host Defense. NIH-NIAAA,

$10,000,000. (12/01/2009 – 11/30/2014)

Mao J (PI, Columbia University. Lopez subcontract). Tracking stem cells in engineered tissue and organs in vivo and in real time.

NIH - NIAMS, $126,765. (Sept. 2009 – Aug. 2014)

Feldman M, Hah D, Lopez MJ, Barton J. R21: Optical probe for microscopic cancer diagnosis in minute structures. NIH-NCI,

$411,327. (April 2009 - April 2011)

Lopez MJ. Demineralized Bone Matrix with Syngeneic Adult Adipose Multipotent Stromal Cells Accelerates Spinal Fusion

Compared to Demineralized Bone Matrix Alone in an Athymic Rat Model, Allosource, $39,248. (12/2010)

Lopez MJ. Intra-articular Filter-derived Platelet Therapy to Alleviate Signs of Equine Osteoarthritis. Pall Corporation, $30,000.

(6/2010)

Lopez MJ. Osteogenic differentiation of equine stem cells (EASCs) in biodegradable scaffolds: a rat model for equine bone

reconstruction. LSU Equine Health Studies Program, $7,500. (7/2010-6/2011)

Lopez MJ, Kaidapuram LRN. Generation of a computer model of equine motion to study therapeutic riding. LSU Equine Health

Studies Program, $3,300. (7/2010-6/2011).

2008-2010 GRANTS

33

Lopez MJ, Pinto V. Isolation and characterization of adult stromal cells from the equine laminae. LSU Equine Health Studies

Program, $8,800. (7/2010-6/2011)

Lopez MJ. Administrative supplement to adult stem cell suppression due to alcohol induced microenvironment alterations. NIH-

NIAAA, $47,040. (4/2010)

Lopez MJ. Comparison of two soft tissue fixation devices for anterior ligament cruciate reconstruction in a canine model. Tesa

Medical, Inc., $71,013. (4/2010)

Lopez MJ, Gimble J. Equine bone regeneration with adult stem cells Grayson Jockey Club Foundation, $157,830. (4/2010

– 3/2012)

Lopez MJ. Travel grant for emerging faculty. LA Board of Regents, $1,200. (2010).

Dr. Sara Lyle:

Beehan, D, Lyle, SK, Eilts BE, Len JJ, Love D. The investigation of the effects of lodixanol during equine semen chilling and

cryopreservation. EHSP Research Funds, $3234.

Len JA, Beehan D, Lyle SK, Eilts BE, Love C. Effect of a cushion solution during centrifugation on equine sperm cells. EHSP

Research Funds, $3558.

Dr. Rebecca McConnico:

McConnico RS, Mirza M, Wolfson W, Poirrier RA, Navarre C, Heidorn N, Miller C, Still R,

State/Regional Disaster Management Training Program grant award, American Veterinary Medical Foundation (AVMF), providing

emergency response training for veterinary students, veterinarians, first responders, and others to aid animals before, during, and

after disasters, $20,000 matched with in-kind gifts for a total value of $63,200, 2010, funded

McConnico RS, Scholarship award for college of veterinary medical faculty, The Chi Institute for Traditional Chinese Veterinary

Medicine, Reddick, Florida, $4,000, 2010, funded

McConnico RS, Teaching grant, LSU SVM professional curriculum and continuing education courses in Disaster Medicine and

Response, International Fund for Animal Welfare, $1,200, 2008, $3,000, 2010, funded

McConnico RS, Mirza M, Wolfson W, Poirrier RA, Teaching grant, LSU SVM professional curriculum and continuing education

courses in Disaster Medicine and Response, LSU’s Stephenson’s Disaster Management Institute, 2008, $2,500, 2009, $2,500,

2010, $1,000, funded

McConnico RS, Paulsen D. The effect of COX1-sparing agents on secretion in the equine right dorsal colon. LSU-Equine Health

Studies Program competitive grants program. $12,344, 2010

McConnico RS, Khodadad J. Examining the pharmacokinetics and safety of firocoxib, a COX-1 sparing NSAID in foals. Merial, Inc.

$13,562, 2009-2010

2008-2010 GRANTS

34

McConnico RS, Andrews F, Eades SC, Roy A, Littlefield MC. Consecutive equine herpes virus infection outbreaks at Louisiana

thoroughbred racing venues. LSU-Equine Health Studies Program, $3500, 2008-2009

Fernandez R, McConnico RS, Koch CE. Effects of Buscopan and flunixin on permeability and secretory capacity of equine right

dorsal colonic mucosa. Louisiana State University, School of Veterinary Medicine Merial Summer Scholars Program, Merial, Inc.,

$5,500, 2010

Khodadad J, McConnico RS. Examining the pharmacokinetics and safety of firocoxib, a COX-1 sparing NSAID in foals. Louisiana

State University, School of Veterinary Medicine Merial Summer Scholars Program, Merial, Inc., $5,500, 2009

Hayden M, McConnico RS, Barker S. Levamisole administration causes equid and bovid urine to test-positive for amphetamine,

Louisiana State University, School of Veterinary Medicine Merial Summer Scholars Program, Merial, Inc., $5,500, 2008

Hosgood, G, Snow L, Davidson J, McConnico RS. Effects of carprofen on canine gut barrier function. LSU SVM Veterinary Clinical

Sciences CORPS Funding, $3,500, 2008

Dr. Laura Riggs:

Evidence for generalized integumentary disruption in naturally-occurring laminitis” Equine Health Studies Program $12,000

Dr. Gary Sod:

Sod G, Durham M, Riggs L. Comparison of extraction torques between plasma sprayed hydroxyapatite coated and uncoated

4.5 mm AO cortical screws used to secure a 4.5mm broad dynamic compression plate (DCP) in equine third metacarpal bones,

$12,000, EHSP

Sod G, Baia P, Riggs L. Comparison of extraction torques between 5.5 mm AO cortical screws with roughened contact surface

and regular 5.5 mm AO cortical screws with polished contact surface used to secure a 4.5mm broad dynamic compression plate in

equine third metacarpal bones. $8,000.00, EHSP

Dr. C.S. Venugopal:

Pharmacological evaluation of potential drugs for equine laminitis: an in vitro study. PI: Venugopal CS. Co-investigators: Beadle RE

and Eades, SC. Agency: USDA Formula Funding, Amount: $12,000; Duration: Sept 1, 2010 to Aug 31, 2011. Received a “no cost

extension” for another year (Aug 31, 2012)

In vitro investigation of insulin resistance on equine digital vessels. PI: Venugopal CS. Co-investigators: Beadle RE and Eades SC.

Agency: Equine Health Studies Program, Amount: 12,000. Duration July 1, 2009 to June 30, 2010.

Identification of a biomarker for recurrent airway obstruction in horses. PI: Venugopal CS. Co-investigators: Eades SC and Beadle

RE. Agency: Equine Health Studies Program, Amount:$12,000. Duration: July 1, 2008 to June 30, 2009.

Role of oxidative stress and endothelin in recurrent airway obstruction in horses. PI: Venugopal CS. Co-investigators: Joseph

Francis and Eades, SC. Agency: Equine Health Studies Program, Amount: $12,000. Duration: July 1, 2007 to June 30, 2008.

2008-2010 GRANTS

35

Interaction of endothelin receptors in equine airways. PI: Venugopal CS. Co investigators: Joseph Francis and Mandi Lopez.

Agency: USDA Formula Funding. Amount: $10,000. Duration: Sep 15, 2007 to Sep 14, 2008.

2008-2010 GRANTS

36

State-of-the-Art Facilities and Equipment

The Equine Health Studies Program is an interdepartmental, multidisciplinary equine biomedical program within the LSU School of

Veterinary Medicine that is dedicated to the health, well-being and performance of horses through veterinary research, education,

and service. A diverse group of faculty, graduate students, post-doctoral fellows and staff conduct cutting-edge research in equine

health and disease. The program maintains a herd of approximately 90 horses and ponies for teaching and research. Three

research barns in the facility contain over 40 stalls and several pastures and paddocks are available for turnout and housing. Since

the last research report (2006-2007) we have upgraded and added some key facilities and equipment. These include the naming

of the EHSP physiology and pharmacology laboratory as the Charles V. Cusimano Equine Physiology & Pharmacology Laboratory,

updating of the Equine Performance Evaluation Laboratory and Lameness Pavilion, the Equine Cell & Tissue Culture Laboratory,

the Laboratory for Equine and Comparative Orthopedic Research, and the Equine Molecular Laboratory. Additionally, the clinical

facilities and equipment within the Veterinary Teaching Hospital and other core research facilities and resources within the School

of Veterinary Medicine support the research activities of the EHSP. The information highlights the updates and facilities since the

last research report.

Charles V. Cusimano Equine Physiology & Pharmacology Laboratory

Numerous faculty and graduate students conduct

scientific investigations involving equine physiology,

pharmacology, and pathophysiology, including, but not

limited to, vascular and nonvascular smooth muscle

physiology, pathology, and pharmacology (digital and

intestinal vasculature, bronchial, uterine and intestinal

smooth muscle), mucosal injury due to non-steroidal-anti-

inflammatory drugs, mucosal physiology and permeability,

endotoxemia, laminitis, and development and testing the

effects of medications on hemodynamics. We currently

have 15 Ussing’s chambers systems and multiple tissue

baths for measuring gastrointestinal mucosal permeability

and injury and muscular contraction.

Ussing’s chambers for gastrointestinal permeability studies

EHSP FACILITIES AND EQUIPMENT

37


Tissue baths in the Charles V. Cusimano Physiology and Pharmacology Laboratory.

Laminitis Research Laboratory

Pfeiffer-Burt Lameness and Performance Pavilion

The Pfeiffer-Burt Lameness and Performance

Pavilion (75’ x 125’) is a modern facility for evaluation

of locomotion in horses. Several research projects

evaluating the efficacy of pharmaceutical and

nutraceutical agents and feed additives for treatment of

lameness in horses are being evaluated in this facility.

Lameness examination in the Pfeiffer-Burt Lameness Pavilion

38

Force Plate Analysis in the Pfeiffer-Burt Lameness Pavilion

Equine Performance Evaluation Laboratory

The Equine Performance Evaluation Laboratory (EPEL) is equipped with a high-speed treadmill for exercising horses at speeds

that mimic racing conditions. A Coda Motion System digital motion analysis system that incorporates markers on the horse into

digital images to evaluate gait and lameness, is also available (see image below). Evaluation of the dynamics of the upper airway

can be achieved by a dynamic endoscopic system. We recently added a dynamic endoscope that can evaluate the upper airway in

research and clinic horses exercising on the high-speed treadmill or working in their natural environment, such as the racetrack or

riding arena.

High-speed treadmill with Coda Motion analysis system attached


39


Dynamic Endoscope

Laboratory for Equine and Comparative Orthopedic Research

The Laboratory for Equine and Comparative Orthopedic

Research was established and designed to facilitate a

strong association between clinical and basic orthopedic

research for advancement of orthopedic knowledge

across species and disciplines. The Laboratory for Equine

and Comparative Orthopedic Research is specifically

designed and equipped for translational orthopedic

research from the molecular/genetic level to the structural

level.

The laboratory is equipped with the most modern

equipment for molecular/genetic work, including an

MJ Research Chromo4 Detector and DNA Engine 200

for DNA fragment amplification and Quantitative PCR,

a UVP hybrilinker for blot analysis and a Synergy HT

multi-detection microplate reader for ELISA assays.

Housed within the laboratory is a Leica DM 4000 light

microscope with fluorescent, polarizing and phase

contrast capabilities. The microscope is equipped with the

latest in digital image capture equipment and software.

Additionally, there is a PathScan Enabler to obtain

ultra high quality images from 1 x 3 inch glass slides. A

custom-designed servohydraulic axial torsional Material

Testing System with a Flex Test SE Controller and equipped with a Multiple Gage Length Axial Extensometer makes nearly any

level of mechanical tissue testing possible from the tissue and bone level to joint and whole limb testing. Presently, several state-

40

of-the-art diamond saws are available for both orthopedic hard and soft tissue microscopic and ultrastructural sample preparation.

A section of the laboratory is devoted to histologic preparation of both calcified and decalcified tissue samples. Areas of research

focus include the pathophysiology of hip dysplasia, the development and implementation of novel orthopedic devices and cranial

cruciate ligament disease. Also, the laboratory is active in equine stem cell research, regenerative medicine and tissue culture.

The laboratory focuses on translation of basic scientific research to the live horse. Projects within the laboratory include growth of

laminar cells for use as an in vitro model of laminitis and bone marrow stem cells for use in tendon healing.

Equine Orthopedics and Biomechanics

We have developed a solid research program in the area of equine orthopedics and biomechanics. This has led to the

development of numerous orthopedic implants designed specifically for equine use, which is critical for the advancement of

equine orthopedics and fracture repair. These equine specific orthopedic implants have distinct advantages over those intended

for human application. The EHSP is unique in that it is the only school of veterinary medicine in the country that is designing and

testing equine specific orthopedic implants. An integral part of this research program was the development of a finite element

computer model that allows for the biomechanical testing of an orthopedic implant applied to an equine bone or bones. This allows

for changes in implant design to be made and tested using the finite element model prior to in vitro biomechanical testing. This

computer aided design approach allows for more efficient use of the limited cadaver specimens. This research has direct and

often immediate clinical applications. We are presently advising and performing biomechanical tests on prototype large animal

orthopedic implants for Synthes Vet, Inc., Innovative Animal Products, and IMEX Veterinary, Inc.

EHSP Clinical Facilities and Equipment

The Veterinary Teaching Hospital is staffed 24/7 with board certified and internationally recognized veterinary specialists (internal

medicine, surgery, anesthesiology, and radiology) and highly-skilled veterinary technicians that provide clinical service to the

horse-owning public. Since the last research report, the LSU Veterinary Teaching Hospital has added a new modern state-of-the-

art MRI unit that for evaluation of soft tissue and bony lesions in the head, neck, and limbs of horses with hard to diagnose clinical

conditions, including foot and lower limb lameness.

MRI Unit

The clinical facility has added new digital endoscopy equipment (Karl Storz, Inc.) to diagnose conditions of the upper and lower

airways, esophagus, stomach and reproductive tract.


41


Diagnostic and Research Endoscopy

Standing endoscopy of esophagus, stomach, and duodenum (see story ‘Researching Gastric Ulcer Disease” on page 60).

Equine Reproduction/Theriogenology Laboratory

The Equine Reproduction/Theriogenology Laboratory has

complete facilities for the evaluation, chill-transport, and

cryopreservation of spermatozoa, including light and phase-

contrast microscopes and a computer-assisted spermatozoal

analysis system (Spermvision®). We have three ultrasound

machines with 5-7.5 MHz linear array transducers and

a 5-7.5 MHz sector array transvaginal transducer for

oocyte collection by follicular aspiration or twin reduction

by aspiration. A fourth ultrasound system equipped with

a 3.5 sector and 5-7.5 microconvex array is available for

transabdominal imaging. An Olympus endoscope is also

available for hysteroscopic examination and for hysteroscopic

low-dose insemination. Laparoscopy is available and used for

oviductal insemination and for minimally invasive placement

of intrauterine catheters. We maintain a close collaborative

relationship with the Equine Biotechnology Laboratory,

which is part of the LSU Agricultural Center. This facility has

tissue culture laboratories and micromanipulators that make

possible such advanced assisted reproductive techniques as

intracytoplasmic spermatozoal injection and nuclear transfer

(“cloning”).

42

Equine Molecular Biology Research Laboratory

The Equine Molecular Biology Research Laboratory is equipped to support the molecular biology aspects of research conducted

by the EHSP investigators. The missions of this laboratory are to perform basic, cutting-edge research to explain the molecular

basis of disease with a view to improved clinical approaches; to train scientists, students, and visitors at all levels; and to develop

new instruments and methods in equine molecular biology. We also have direct access to the core facilities within the Division of

Biotechnology and Molecular Medicine (BIOMMED) within the LSU School of Veterinary Medicine for quantitative Real-Time PCR,

primer/probe design, Quantity One for DNA fragment visualization and analysis, SDS-PAGE analysis, MagnaPure automated

nucleic acid extraction, and microarray spotters and readers. Current investigations utilizing this laboratory include study of key

mediators in equine laminitis, metabolic disease, gastrointestinal disease, summer pasture-associated recurrent obstructive airway

disease, bone healing and mechanisms of pain sensation and modulation. The capabilities of this laboratory have expanded in light

of the increasing importance of the molecular biological approach to the investigation of equine health and disease.


43

Disaster Preparedness

LSU Veterinary School associate professor speaks at World Congress on Disaster and Emergency Medicine

The 17th World Congress on Disaster and Emergency

Medicine (WCDEM) closed on June 3. Held in Beijing,

China, the Congress hosted more than 1,600 participants

from 57 countries.

The 17th WCDEM was held in conjunction with the 14th

Annual Meeting of the Chinese Society for Emergency

Medicine. With the scale and frequency of disasters

increasing, the 17th WCDEM was more relevant than

ever. This year alone marks one of the worst in terms of

disasters—the earthquake, tsunami, and nuclear reactor

issues in Japan; floods in Australia; earthquakes in New

Zealand; and floods and tornados in the United States.

Research, experiences, and lessons learned from these

recent events, and many others, were presented by

delegates at the 17th WCDEM.

LSU School of Veterinary Medicine’s expanding disaster

training program was highlighted with two presentations

by Rebecca S. McConnico, DVM, PhD, DACVIM (LSU

SVM 1987), associate professor of veterinary medicine

in the Department of Veterinary Clinical Sciences. Dr.

McConnico’s presentations were added as part of a

special veterinary component of this international meeting

due in part to expanding global concerns about societal

responsibilities for including animals in local, regional, and

federal disaster planning. Dr. McConnico’s presentation topics were biosecurity considerations for equine emergency sheltering

and experiential learning in disaster response for veterinary students and veterinarians.

RESEARCH NEWS & DEVELOPMENT

TOP: Dr. Rebecca McConnico, associate professor of veterinary medicine at LSU’s School of Veterinary Medicine, and Dr. Dick Green, international director of emergency response, International Fund for Animal Welfare, in front of the conference banner in Beijing, China.

BOTTOM: Attending the World Congress on Disaster and Emergency Medicine are (from left) Dr. John Madigan, Medicine, professor of equine medicine, University of California Davis, School of Veterinary; Dr. Heather Case, director of the American Veterinary Medical Association’s Scientific Activities Division and the Louisiana State Animal Response Team coordinator of emergency preparedness and response; Dr. Rebecca McConnico, associate professor of veterinary medicine at LSU’s School of Veterinary Medicine; and Dr. Diane McClure, associate professor, Western University of Health Sciences.

44

LSU Veterinary School dedicates laboratory in honor of Charles V. Cusimano

The LSU School of Veterinary Medicine recently dedicated the Equine Physiology and Pharmacology Laboratory in honor of

Charles V. Cusimano. “Our purpose in recognizing Mr. Cusimano is to say, ‘Thank you’ for his enduring support and valuable

efforts to the school and the Equine Health Studies Program,” said Frank Andrews, DVM, PhD, director of the EHSP.

Mr. Cusimano’s family and friends, as well as many LSU faculty and staff, gathered at the LSU SVM on March 3 for the dedication

ceremony. Dean Peter F. Haynes served as master of ceremonies. “Today is all about Charles V. Cusimano, his passion and

support for LSU and the School of Veterinary Medicine, and his central and pivotal role in establishing our Equine Health Studies

Program,” said Dean Haynes.

Representing the LSU System was President John Lombardi, and representing the LSU Baton Rouge campus was Chancellor

Mike Martin, who said, “Great universities are known by the company they keep, and it’s great to keep the kind of company that

the Cusimanos bring to this campus. It sends a message to those folks out there that this is a first-rate place because we deal with

first-rate people.”

Dr. Mike Strain (LSU SVM 1983), Louisiana Commissioner of Agriculture & Forestry, told the crowd about working with Mr.

Cusimano to secure dedicated funds for the EHSP from slot machine proceeds from Louisiana’s racetracks. “It took seven years to

make this work,” said Dr. Strain. “One year we got the bill going, but it was bogging down. In 2003, Charlie comes to me and says,

‘We’re going to see Mike [Foster].’ We went to see the governor and got his support, but the bill kept getting referred to different

committees. On the last day, at the last hour, we got the bill through and began a research program that has paid huge, huge

dividends.”

Charles Vincent Cusimano, a native of New Orleans, Louisiana, graduated from Louisiana State University with a BS in petroleum

Photo: From left, Dean Peter F.

Haynes and Commissioner of

Agriculture & Forestry Mike Strain

(LSU SVM 1983) congratulate

Charles Cusimano during

the dedication of the Equine

Physiology and Pharmacology

Laboratory. Mr. Cusimano was

joined on stage by his wife, Violet

Cusimano.


45

engineering in 1951. He attended LSU on a football scholarship, lettered for four years, and played in the Cotton Bowl in 1946 and

the Sugar Bowl in 1950. Cusimano also studied law at Loyola University in New Orleans and did postgraduate work in geology at

the University of Oklahoma.

Cusimano is a former member of the Louisiana State University Board of Supervisors who began his service in 1974 and served as

chairman in 1995-1996. He was admitted to the LSU Law Hall of Fame in 1987 and the Engineering Hall of Distinction in 1999. In

addition, he helped establish religious centers on all of LSU’s campuses.

Photo: Charles Cusimano cuts the

ribbon to the Equine Physiology

and Pharmacology Laboratory,

which has been dedicated in his

honor, alongside his wife, Violet

Cusimano, and LSU System

President John Lombardi.


46

How You Can Support the EHSP and Enhance the Health, Well-Being and Performance of Horses

There are many ways individuals, foundations and companies can help support the LSU School of Veterinary Medicine’s Equine

Health Studies Program. The EHSP is “dedicated to the health, well-being and performance of horses through veterinary research,

education and service.” In order to fulfill our mission of becoming one of the premier equine biomedical centers in the country, we

have initiated a campaign to generate funds to enhance all aspects of our program.

Our endowment is comparatively small, so each gift is extremely special to us and will make an important and immediate impact on

our programs. Our fundraising efforts have been principally through private, charitable, tax-deductible gifts, as well as some other

special events and activities. All gifts are tax-deductible and can be pledged with a portion given annually over a period of a few

years. We hope that you will consider assisting us with our fundraising efforts for facility enhancements, endowed/distinguished

professorships and chairs, and/or scientific advances.

An endowed gift is a permanent gift. The principal is invested and returns annual interest. Part of the annual interest is reinvested

to increase the principal, and part is used for the intended purpose (such as a professorship/chair or research). Endowed funds are

usually named for the benefactor or for a designated honoree.

Professorships and Chairs

The state of Louisiana has a matching program for Endowed Professorships and Endowed Chairs. The School currently has

only one Endowed Chairs and three Endowed Professorships, none of which are in the area of equine health and performance.

Endowed positions are vital to our instructional and investigational programs. The individuals in these positions will serve as

leaders of teams of equine clinicians and investigators that conduct cutting-edge scientific investigations.

Equine Biomedical Research

Private gifts can provide funds for leading-edge science specific to the horse. With the limited amount of state and federal funding

available for equine scientific investigations, funds from private, charitable gifts are critical to improve successful prevention and

treatment of illnesses and injuries that can be performance-limiting, career-ending and even life-threatening.

General EHSP Support Fund

Gifts can be made to the General EHSP Support Fund (a non-endowed account). These funds may be used to purchase

equipment for equine scientific investigations. Additionally, they are used for continuing education for the horse-owning public and

private equine veterinarians. These funds also are used to assist with other educational, promotional and fundraising activities.

Equine Farrier Unit

Gifts can be made to the Pfeiffer-Burt Lameness and Farrier Unit. These funds will be used to supplement current funding to

provide equipment for diagnosis and treatment of medical and surgical conditions of the equine hoof.

Equine Reproduction

Gifts can be made to the EHSP Equine Reproductive Center. These funds will be used to construct a state-of-the-art equine

reproductive center that will provide diagnosis and treatment of horses with medical or surgical problems that lead to infertility or

decreased conception rates.

EHSP DEVELOPMENT

47

EHSP DEVELOPMENT

Memorial Gifts and Naming Opportunities

Your gift may be used to honor or memorialize a beloved horse, family member, or friend. Naming opportunities exist for endowed

gifts like scholarships, professorships and chairs. Newly constructed facilities and novel projects offer a wide variety of naming

opportunities, including the Equine Intensive Care Unit and the Equine Reproduction Unit.

Again, any gift will be very special to the LSU School of Veterinary Medicine’s Equine Health Studies Program and will make a

dramatic and immediate impact on our teaching, service and scientific investigation programs. We thank you for your generosity

and support. To learn more about how your gift will assist the EHSP with its mission, please visit our website (www.equine.vetmed.

lsu.edu) or contact Ky Mortensen via telephone (225-578-9590) or e-mail ([email protected]).

I want to support the LSU SVM EHSP!Please use my gift to continue the excellence in community service, education, and research through the EHSP.

___ $500 ___ $250 ___ $100 ___ $________ Other

Your company may provide corporate matching funds. Please contact your employer’s human resources office for more information.

Name ___________________________________________________________________LSU SVM Graduation Year_______________

Home / Work (please circle) Address___________________________________________________________________________

__________________________________________________________________________________________________________

Home Phone________________ Work Phone_________________ E-mail______________________________________________

For credit card contributions: _____VISA _____MC _____Discover _____ AMEX

Credit Card Number______________________________________________________Expiration Date______________________

Cardholder’s signature _____________________________________________________________________________________

_____ I would like to be contacted about including the LSU SVM in my estate plans.

_____ I have included the LSU SVM in my estate plans.

Please make your check payable to: LSU FoundationMail to: Louisiana State University School of Veterinary Medicine Institutional Advancement Baton Rouge, LA 70803

Please use this form to make your gift, or call 225-578-9948 to make your gift by phone!

Visit https://www.lsufoundation.org/contribute.php to give online.

48

BIOMECHANICS AND ORTHOPEDICS

BIOMECHANICAL ALGORITHM TO QUANTIFY FORCES FROM EQUINE MOTION AT THE SADDLE POSITION

Authors/Investigators

Laxmi R Nandana Kaidapuram, BTech,MS; Mandi J Lopez, DVM, MS; Lin Xie, BS

Description of the Problem

There is no scientific method or algorithm in literature to determine the most appropriate horse for each individual based on

functional parameters of the horse and the rider in therapeutic riding.

Study Purpose/Objectives

Therapeutic horseback riding is a common component of many physical therapy programs. Forces transferred to the rider vary with

horse size, breed and gait. Interaction between a horse and rider is a complex problem of coupled dynamic body interactions. The

potential forces transferred to the rider may be predicted by quantification of the forces on to a static weight from the horse back at

the level of the saddle. To accomplish this task, an algorithm to quantify forces at the saddle level from ground reaction forces was

developed using a combination of direct and indirect dynamic analysis to test the hypothesis that the forces transferred to a static

weight at the saddle level on can be predicted by the breed and weight of the horse.

Approach

Seven adult horses were used for data collection: 3 Thoroughbreds, 3 Quarter Horses and 1 Paso Fino (4 geldings and 3 mares),

5-14 years; 387-580 kg. Simultaneous, real time kinetic, kinematic and back force data on a static weight was collected at a

walk using an integrated system consisting of a force platform, active motion detection system, and wireless force transducers,

respectively. Infrared light emitting markers were glued to the skin at 22 specific anatomical landmarks. A customized surcingle

fixture to hold a 12kg weight on top of wireless force transducers were used for data collection at the saddle level. Kinematic data

and ground reaction forces (GRF) were collected. Forces at the saddle level were measured with a wireless load force system

which was synchronized with the force platform and motion capture system using a double mouse. Data was collected from a

minimum of four trials from all horses at a walk (1.3-2.0 m/s). Inverse dynamic analysis was used to calculate the fore and hind limb

joint forces to the level of the shoulder and hip. Virtual segments were created from the shoulder to the spine, and the segments

were modeled as series of springs and dampers. The energy absorption coefficients were derived through a series of iterations to

align the inverse dynamic solutions of the fore- and hind limb data with the forces measured at the back.

Results/Conclusions

Forces at the saddle level on to a 12 kg. weight are shown in Figure 1 for three breeds of horses. For all the horses, the first peak

value occurs within the first 25- 50% of the stride cycle. Though the overall pattern of the force is same for all the horses, there is

significant variation in peak and valley values from breed to breed and horse to horse. Peak-to-peak force on the 12 kg. weight

is proportionated to 60 kg., which is equivalent to an average rider weight. Paso Finos produce forces at the saddle level that are

115% more than Thoroughbreds and 45% more than Quarter Horses. Forces at the saddle level are based on breed of the horse,

and it doesn’t show any significant variation with weight of the horse. In contrast, ground reaction forces depend on the weight of

the horse irrespective of the breed.

49


Acknowledgements

This study was supported by the EHSP.

Year Completed

2011

Published Manuscripts/Abstracts

Laxmi R Nandana Kaidapuram,BTech,MS; Mandi J Lopez,DVM,MS; Lin Xie, BS, Biomechanical algorithm to quantify forces from

equine motion at the saddle position. A poster was presented at the 2011 meeting of the American College of Veterinary Surgeons.

Forces at the saddle level on a 12 kg. weight at the rider level of Thoroughbreds, Quarter Horses, and Paso Fino.

Thoroughbred Horses

Quarter Horses

Paso Fino

50

An in vitro biomechanical comparison between dynamic compression plates having a roughened contact surface and dynamic compression plates having a polished contact surface for the fixation of osteotomized equine third metacarpal bones

Authors/Investigators:

Gary A. Sod, DVM, PhD, Laura M. Riggs, DVM, PhD, Diplomate ACVS, Colin F Mitchell, BVMS, MS, Diplomate, ACVS, George S.

Martin, DVM, MS, MBA, Diplomate, ACVS, Marjorie S. Gill, DVM, MS, Diplomate, ABVP

Year Completed:

2010

Published Manuscripts/Abstracts:

Sod GA, Riggs, LM, Mitchell CF, Martin GS, Gill MS. An in vitro biomechanical comparison between dynamic compression

plates having a roughened contact surface and dynamic compression plates having a polished contact surface for the fixation of

osteotomized equine third metacarpal bones. Veterinary Surgery, 40(4), 2011.

Sod GA, Riggs LM, Mitchell CF, Martin GS, Gill MS. Why do orthopedic implants fail in equine long bone fracture patients? - Part I

and Part II. Veterinary Orthopedic Society 35th Annual Conference, Big Sky, MT, March 2008.

A mechanical comparison of equine proximal interphalangeal joint arthrodesis techniques: an axial locking compression plate and two abaxial transarticular cortical screws versus an axial dynamic compression plate and two abaxial transarticular cortical screws


Gary A. Sod, DVM, PhD, Laura M. Riggs, DVM, PhD, Diplomate ACVS, Colin F. Mitchell, BVMS, MS, Diplomate ACVS, George S.

Martin, DVM, MS, MBA, Diplomate, ACVS

Year Completed:

2010


51


Sod GA, Riggs, LM, Mitchell CF, Martin GS. A biomechanical comparison of equine proximal interphalangeal joint arthrodesis

techniques: an axial locking compression plate and two abaxial transarticular cortical screws versus an axial dynamic compression

plate and two abaxial transarticular cortical screws. Veterinary Surgery, 40(4), -, 2011.

Sod GA, Riggs, LM, Mitchell CF, Martin GS. A biomechanical comparison of equine proximal interphalangeal joint arthrodesis

techniques: an axial locking compression plate and two abaxial transarticular cortical screws versus an axial dynamic compression

plate and two abaxial transarticular cortical screws. Proceedings of the World Veterinary Orthopedic Congress/Veterinary

Orthopedic Society 37th Annual Conference, Breckenridge, CO, February, 2010.

An In Vitro Cyclic Fatigue Comparison of a Modified 5.5 mm Locking Compression Plate Fixation with a 5.5 mm Locking Compression Plate Fixation of Osteotomized Equine Third Metacarpal Bones


Gary A. Sod, DVM, PhD, Laura M. Riggs, DVM, PhD, Diplomate ACVS, Colin F Mitchell, BVMS, MS, Diplomate, ACVS, George S.

Martin, DVM, MS, MBA, Diplomate, ACVS, Marjorie S. Gill, DVM, MS, Diplomate, ABVP

Year Completed:

2009




39(7), 833-838, 2010.


52

In Vitro Tissue Generation by Adult Equine Multipotent Stromal Cells on Collagen Scaffolds


Lin Xie, BS; Anna Marsano, PhD; Yanru Zhang, PhD; Jeffrey Gimble, MD, PhD; Gordana Vunjak-Novakovic, PhD; Mandi J. Lopez,

DVM, MS, PhD


Adult equine multipotent stromal cell (MSC) research supports the potential of the cells to restore normal tissue structure and

function when combined with biocompatible scaffolds. Therefore, there is great potential to accelerate tissue repair with equine

stromal cell therapy in combination with other well-established medical and surgical techniques. To derive the greatest benefits

from equine stromal cell applications and propel the technology into clinical trials, it is necessary to optimize equine MSC-

scaffold constructs in vitro. Adult equine adipose- (ASC) and bone marrow-(BMSC) derived multipotent stromal cell adipogenesis,

osteogenesis and chondrogenesis on commercially available bovine collagen I (COLI) scaffolds were quantified in this study.


The overall goal of this study was optimization of adult equine multipotent stromal cell interactions with biocompatible scaffolds for

purposes of advancing clinical application of equine tissue regeneration technology. Several hypotheses were tested to support

this goal. The first hypothesis was that equine BMSCs and ASCs have indistinguishable cell seeding, attachment, viability, and

distribution on COLI scaffolds. The second, two-part hypothesis was that BMSC – COLI scaffold constructs are superior to ASC –

COLI scaffold constructs for osteogenesis and chondrogenesis while ASC – COLI scaffold constructs demonstrate higher superior

adipogenesis.

Approach

Cells in this study were isolated from 4 adult Thoroughbred horses from 7 to 10 years old. Bone marrow (BM) was collected

from sternebrae and adipose tissue was collected from superficial gluteal fascia. The collection procedure was approved by the

Institutional Animal Care and Use Committee. The cell surface marker profile of loaded cells was established just prior to loading

by flow cytometry. Adult equine BMSCs and ASCs were loaded into COLI scaffolds (10x7x3mm) at a loading density of 106 cells/

ml by perfusion bioreactors (Figure 1). Scaffolds were equally divided into sections which were subsequently cultured for 7, 14 or

21 days in stromal or adipogenic, osteogenic, or chondrogenic medium in triplicate. The mRNA levels of tissue specific genes of

cell-scaffold constructs were quantified by RT-PCR. Cell-scaffold constructs were also assessed for cell distribution and viability,

cell number, microstructure, and ultrastructure by confocal laser microscopy, DNA quantification, light microscopy, and scanning

electron microscopy, respectively. All values are reported as mean ± standard error (SE). Student’s t test was performed to

compare the effects of cell type on cell loading efficiency. Three-way ANOVA was used to compare the effects of cell type, post-

loading culture time and induction medium on the cell viability. Different combination effects were evaluated with Tukey’s post-hoc

tests. Significance was considered at P<0.05.

Results/Conclusions

Loading efficiency was 74.6% ± 14.4% and 85.8% ± 9.2% for BMSCs and ASCs, respectively. Both cell types were positive for

CD29, CD44, and CD105, and BMSCs were additionally positive for CD73 and CD90. Cells were uniformly distributed in the

scaffold immediately after loading. The mRNA levels of collagen I and sex determining Region Y-box 9 (Sox9) were significantly

STEM CELL AND REGENERATIVE MEDICINE

53

STEM CELL AND REGENERATIVE MEDICINE

higher in BMSC constructs while lipoprotein lipase (LPL) mRNA levels were significantly higher in ASC constructs at all time points

(Figure 2). Tissue specific micro- and ultrastructural changes were evident in cell-scaffold constructs cultured in induction versus

stromal medium (Figure 3).

Benefits to/Impact on the Equine Industry

This study evaluated the in vitro behavior of two types of adult equine MSCs on COLI scaffolds. Both cell types had high

viability and proliferation, confirming the suitability of COLI scaffolds for cell growth. Furthermore, COLI scaffolds supported cell

differentiation into distinct mesenchymal tissues. This systematic and comprehensive in vitro evaluation of interactions between

COLI scaffolds and equine MSCs proved their potential to support diverse mesenchymal tissue generation for equine industry.

Take Home Message

The versatility and suitability of type I collagen scaffolds for equine adult MSC tissue regeneration was confirmed by parallel

generation of distinct mesenchymal tissues by paired ASCs and BMSCs.

Acknowledgements

Funding for this project was provided by the LSU Equine Health Studies Program.

Year Completed

2010–2011

54

ISOLATION AND CHARACTERIZATION OF EQUINE LAMINAR PROGENITOR CELLS


Vanessa Marigo, DVM; Marilyn A. Dietrich, BS, MSc; Frank Andrews, DVM, MS; Jeffrey M. Gimble, MD, PhD; Mandi J. Lopez,

DVM, MS, PhD, DACVS


The laminar tissue is a very unique structure that balances forces within the equine hoof. It has two major components, the dermal

component, attached to the coffin bone, and the epidermal component, attached to the hoof wall, preventing the distal phalanx

from rotating in response to tension from the deep digital flexor tendon. Failure of this complex relationship results in laminitis. This

disease affects about 15 % of horses in the United States each year, and 75 % of those affected, more than 8,000, lose their lives.

Costs associated with this devastating condition exceed $10 million per year for diagnosis, treatment, and lost revenues. Laminitis

is a multifactorial disease making identification of specific causes and treatment difficult. Knowledge of equine laminar cells

physiology will contribute to understand, prevent or reverse the damage caused by laminitis.


The objective of this project was to isolate primordial cells from equine laminar tissue characterize the proliferation rate, identify cell

surface markers expression and estrogenic differentiation potential in vitro.

Approach

Cells were isolated from laminar tissue from horses euthanized for reasons other than the present research. The in vitro expansion

characteristics of cells were determined with cell doubling number (CD) and doubling time (DT) assays. Limit dilution assays

were used to quantify colony forming units for fibroblasts (CFU-F). Cells from passages 1 and 3 cultured in stromal medium were

submitted to flow cytometry of fluorescein isothiocyanate (FITC) and phicoerithrin (PE) labeled cells to identify CD44, CD29 and

CD105, which are progenitor cells surface markers. To address the osteogenic potential cells isolated from the laminar tissue

were maintained in stromal media until 70% confluence, when media was changed to osteogenic induction media (DMEM-Ham’s

F12, 10% FBS, 1% antibiotic/antimycotic solution, β-glycerophosphate (10 µmol/L), dexamethasone (20 nmol/L), and sodium

2-phosphate ascorbate (50µg/mL). Cells were then cultured for 21 more days and media were. Cells were rinsed with 150mM NaCl

3 times and fixed in 70% ETOH. After fixation, cells were stained with a 2% Alizarin Red solution in distilled water.

Results/Conclusions

Cells had similar proliferation rates from passages 1 to 6. The overall mean ± SEM CD for P1 was 1.14±0.26 and P6 was

1.26±0.16 and DT for P1 was 2.30±0.57 and P6 was 5.19±1.14 days. The cells do not double as efficiently in later passages

compared to earlier passages, however this difference is not statistically significant. Primary fibroblastic phenotype (CFU-F)

occurred at a frequency of 1 per 552 nucleated cells, 1 per 302 nucleated cells and 1 per 457 for passages 1, 3 and 6 respectively.

Cells from passage 1 and 3 were had more than 70% positive staining for CD44, CD29 and CD105 suggesting these cells are

progenitor cells. After osteogenic induction cells then formed round colonies that stained with alizarin red indicating calcification.

LAMINITIS

55

LAMINITIS

Take Home Message

Our study showed that the cells harvested from equine laminar tissue have a constant proliferation rate in vitro until passage 6,

express specific cell surface markers and can be differentiated into osteoblasts. The significance of this project is that isolation and

characterization of adult stromal cells from the equine laminae will provide a new target area for research efforts to prevent and

cure the disease. The findings have significant potential to enhance understanding and treatment of equine laminitis. Moreover, it

can be a new model to investigate laminitis in vitro.

Acknowledgements

This study was supported by LSU Equine Health Studies Program.

Year Completed

2011


Marigo, V., DVM, Gimble, J.M., MD, PhD; Andrews, F., DVM, MS; Lopez, M.J., DVM, MS, PhD, DACVS. Characterization of

Stromal Cells from Equine Laminae in vitro. Abstract at ACVS Veterinary Symposium, 2011.

Figure 1. Cell Doublings and Doubling Times. All values reflect one mean ± standard deviation.

Figure 2. Flow Cytometry and Osteogenesis. Representative graph of cells stained with CD44 (left) and alizarin red staining calcium deposition after osteogenic induction (right).

56

LAMINITIS

Treatment of Experimental Equine Laminitis with Doxycycline


Susan Eades and Lee Ann Fugler

Matrix metalloproteinases (MMPs) are enzymes present normally in connective tissue including the laminar tissue that holds the

coffin bone into the hoof capsule. These enzymes breakdown proteins in collagen to remove connective tissue during normal

processes of hoof growth and repair. However, excessive matrix metalloproteinase activity is thought to lead to excessive

breakdown of laminar tissue allowing deviation of the coffin during laminitis. Oxytetracycline is an antibiotic that has other

properties in addition to its ability to kill bacteria. Oxytetracycline is also capable of inhibition of MMP enzymes allowing it to be

used in people with severe gingival disease and other disease where excessive collagen breakdown occurs. As a result the partial

success associated with use of oxytetracycline in people, practitioners have employed doxycycline for use in horses with laminitis.

The purpose of this study was to determine the effectiveness of doxycycline, oxytetracycline, and pentoxifylline (PTX) in the

treatment of equine laminitis using a corn starch overload (CSO) model.

The administration of PTX significantly decreased the severity of lameness

associated with laminitis (Fig 1). Doxycycline and oxytetracycline administration did

not alter the severity of laminitis caused by corn starch. Pentoxifylline reduced MMP

production; however, results of these studies also suggest that PTX also decrease

laminitis pain by reducing inflammation.

Figure 1. Lameness grades associated with laminitis in untreated (control) horses and horses treated with pentoxifylline (PTX)

Pentoxifylline is a medication used in people to reduce some of the

symptoms of muscle cramping and pain associated with arterial diseases.

This medication has also demonstrated the ability to reduce scar tissue

formation in chronic diseases of the kidney and lungs. Pentoxifylline

decreased the severity of lameness associated with laminitis induced by

corn starch. The therapeutic effects of PTX in CHO may involve MMP

inhibition and antiinflammatory effects. Pentoxifylline can be used by

veterinarians to reduce the severity of laminitis associated with intestinal

diseases and metritis.

57

GASTROINTESTINAL

Pharmacokinetics and clinical safety of oral paste firocoxib, a COX-1 sparing NSAID, in foals


McConnico RS, Khodadad JL, Koch CE

This study evaluated pharmacokinetics and clinical safety of an oral paste formulation of a commercially available COX1-sparing

NSAID in clinically healthy pony foals in a randomized controlled clinical trial. Values for complete blood count, serum chemistry

profile, urinalysis, pharmacokinetic assay, and gastric endoscopy were evaluated in eighteen Shetland pony foals treated with

firocoxib (0.1 mg/kg, PO, q 24 h) or placebo for 14 days. Foals were divided into 3 treatment groups. Group 1 and 2 foals received

firocoxib while a 3rd group was administered an oral placebo. Gastric endoscopy was performed on group 1 and 3 foals prior to

treatment and on days 7 and 14 to monitor for the presence of gastric ulcers. Group 2 and 3 foals had blood and urine samples

taken sequentially for pharmacokinetic analysis, CBC, serum chemistry evaluation, and urinalysis. Physical examinations were

performed prior to treatment and daily for 17 days. Data were analyzed using anova and paired t-tests (P<0.05). None of the foals

presented adverse clinical effects. There were no significant changes in CBC, biochemical profiles within groups, or differences

between groups. Pretreatment gastric endoscopy scores were not significantly different from evaluations at 7 and 14 days.

Firocoxib was quickly absorbed with an observed maximum concentration at 2 hr, the first sampling interval, for the majority of

animals. Firocoxib plasma concentrations decreased in a log-linear manner after reaching the maximum concentration and steady

state concentrations were achieved by the 7th dose. Based on the sampling times after the final and 14th dose, an average half

life of 1.3 days was estimated. Administration of firocoxib did not cause any adverse effects on gastrointestinal, or hematological or

serum biochemical variables, appears to have been well tolerated, and follows a predictable pharmacokinetic pattern in 4-6 week

old foals.

Right Colonic Pathophysiology in Horses Administered Phenylbutazone


Rebecca S. McConnico, DVM, PhD; Timothy W. Morgan, DVM, PhD; Cathleen C. Williams, MS, PhD; Jeremy D. Hubert, BVSc,

MRCVS; Rustin M. Moore, DVM, PhD


To determine pathophysiologic effects of phenylbutazone (PBZ) on equine right dorsal colon (RDC).

Approach

Animals—12 healthy adult horses.

Procedures—A controlled cross-over observational study was conducted. Clinical and serum variables, colonic inflammation

(histologic grading) and measurement of myeloperoxidase [MPO] activity, malondialdehyde [MDA] concentrations, and

prostaglandin E2 [PGE2] concentrations), ingesta volatile fatty acid (VFA), and arterial blood flow in the RDC were evaluated for a

58

GASTROINTESTINAL

21-day period in horses administered PBZ (8.8 mg/kg, PO, q 24 h) or a control substance.

Results—Data from 8 horses were used for analysis. Plasma albumin concentrations decreased significantly from days 10 to 21

during PBZ treatment, compared to during the same days for control treatment. Phenylbutazone treatment caused neutropenia (<

3.0 x 103 cells/αL). No other clinical or hematologic abnormalities were detected between PBZ or control treatments. Two horses

developed colitis while on PBZ. No significant differences were detected in the RDC between PBZ and control treatments for MPO

activity, MDA and PGE2 concentrations, or for histologic evidence of inflammation. Arterial blood flow in the RDC was significantly

increased during PBZ treatment, compared with values for controls. Differences were identified in VFA production during PBZ

treatment, compared with control treatment, with a decrease in acetic acid concentrations over time.

Results/Conclusions

Prolonged phenylbutazone administration caused hypoalbuminemia, neutropenia, changes in RDC arterial blood flow, and

changes in VFA production. Equine veterinarians should monitor serum albumin concentrations and neutrophil counts and be

extremely cautious when making dosing recommendations for phenylbutazone treatment.


Am J Vet Res. 69(11):1496-505, 2009.

Acknowledgements

Support for this project was provided by the Grayson Jockey Club Research Foundation Incorporated.

New Approaches in Treating Equine Gastric Ulcer Syndrome


Dr. Frank Andrews, Dr. Michelle Woodward, Dr. Patrick Loftin, Dr. Joshua

Cartmill, Dr. Nan Huff, Michael L. Keowen, Frank Garza, Jr.

EGUS is a common problem in horses. All ages and breeds are

susceptible, but the prevalence is especially high in Thoroughbred race

horses, probably because of the intense exercise they undergo during

training and racing. It is estimated that 60% to 90% of performance horses

(between 500,000 and 700,000 animals in the U.S. alone) are affected by

EGUS, which translates to millions of dollars of lost revenue each year in

training and racing days and in the cost of treatment. In addition, EGUS

can be a painful condition, causing considerable suffering among affected

horses and foals.

59

EGUS is a complicated syndrome with a number of potential causes, but risk factors include stress, being transported, diet,

stall confinement, intermittent feeding or fasting, intense exercise, racing, and the use of non-steroidal anti-inflammatory drugs

(NSAIDs). Clinical signs of EGUS, which are present in about half of the horses diagnosed with EGUS, tend to be vague and

include abdominal pain or colic, diarrhea, rough hair coat, poor appetite, depression, and decreased performance. It is poor

performance that makes EGUS an economically significant condition in the racehorse industry,

There are a number of commercial antiulcer drugs that can be used to treat EGUS, such as equine formulations of omeprazole

and ranitidine (both of which are also used in human medicine). But these drugs are expensive—often costing $40 or more per

day—and must be used for at least 28 days for complete healing. This can cause a financial strain for horse owners, who may not

be able to afford to treat their animals for the prescribed length of time, or may use the drugs at sub-therapeutic doses, or even

substitute other medications or feed supplements that are ineffective. Hence, the prevalence of EGUS remains high even with

effective treatment options available.

Dr. Andrews has been looking for a more cost-effective means of managing EGUS, which led him to explore alternative

approaches. In the veterinary community as well as in human medicine, there is increasing interest in herbs and other plants

that have therapeutic applications, and one such plant that shows promise for treating EGUS is the seabuckthorn, Hippophae

rhamnoides.

The seabuckthorn is a deciduous berry-producing shrub that grows in high elevations across Eurasia from the Atlantic coast of

Europe to northwestern China. Since antiquity the plant has been associated with improving the health of horses; in fact, the genus

name Hippophae is Latin for “shining horse,” alluding to the beautiful coats of horses that feed on it.

A liquid equine feed additive derived from seabuckthorn berries, SeaBuck Complete™, is now available, and Dr. Andrews has

been investigating its efficacy in treating and preventing gastric ulcers in Thoroughbreds. “Seabuckthorn berries are highly

nutritious,” said Dr. Andrews, “and are a very good source of vitamins, trace minerals, amino acids, and antioxidants. Extracts

from these berries have been used to successfully treat mucosal injury in humans and rats, so we are looking at this product

as an alternative to omeprazole in treating EGUS.” In a small study involving eight adult female horses, he found that SeaBuck

Complete™ seemed to prevent existing ulcers from worsening under stress, which commonly happens among performance

horses.

Now he wants to continue investigating the use of seabuckthorn products using SeaBuck™ Gastro Plus, a new product from the

manufacturers of SeaBuck Complete™, to see if his initial findings will be confirmed. He has submitted a pre-proposal for a Morris

Animal Foundation grant to expand his research and hopes to start a follow-up clinical trial in the near future.

In addition, Dr. Andrews will be mentoring a student in the Merial/NIH Veterinary Scholars Program this summer. Alyssa Auer, who

is finishing her first year at Purdue University School of Veterinary Medicine, will be at LSU SVM for a 12-week research program

beginning in May, 2010, and will be working closely with Dr. Andrews on a project entitled, ““Efficacy of seabuckthorn berry pulp

and oil on treatment and prevention of gastric ulcers in horses.”

If Dr. Andrews’s research confirms the value of seabuckthorn extracts in managing EGUS, it will be a boon for horses and their

owners alike. At recommended doses, seabuckthorn supplements cost much less than the price of a course of omeprazole,

making it more likely that horse owners will be able to continue treatment for as long as necessary. “We expect seabuckthorn-

treated horses will have significantly fewer and less severe gastric ulcers on endoscopic examination than untreated controls,” he

said. “We believe this research is likely to produce an immediate beneficial outcome on the morbidity caused by gastric ulcers in

performance horses.”

GASTROINTESTINAL

60

DISASTER RESPONSE

Experiential learning in disaster response for veterinary students and veterinarians


McConnico RS, Wolfson W, Poirrier RA, Taboada J, Woodward M, Loftin P, Cartmill J, Huff J, Keowen M, Garza F

The Louisiana State University School of Veterinary Medicine has taken advantage of its physical presence amidst a disaster

prone region of the United States (US) to incorporate veterinary students, faculty veterinarians, and staff as first responders as a

means of experiential training for animal disaster response. Through a solid partnership with the Louisiana State Animal Response

Team (LSART) and others including the LSU Fire and Emergency Training Institute (LSU FETI) and humane organizations, this

veterinary school has developed a disaster response program that includes animal emergency response planning, evacuation,

sheltering, emergency triage, and technical rescue expertise. Specific debriefing sessions from each of the disaster response

scenarios have enabled the emergence of a functional all-hazards disaster response plan and specialized responder training.

Over the past decade, this team has worked closely with local and regional resource providers to develop improved response

activities to care for animals and the people who care for them including Tropical Storm Allison (2001), Hurricanes Katrina & Rita

(2005), Hurricanes Rita 7 Ike (2008), North Louisiana Flood (2009), and the Deep Water Horizon Gulf Oil Spill (2010). These

experiential learning opportunities by way of organized community engagement have provided rigorous training opportunities for

veterinary students and veterinarians at LSU surrounding area. These recent response activities where veterinary students and

veterinarians were able to provide the work force and work first hand with certified emergency responders to provide and training

activities demonstrate a successful model for veterinary training providing robust training experiences and subsequent course

development to address identified gaps in veterinary disaster response training.

61

SURGERY

LARMoF’s Procedure for Treatment of Cribbing in Horses: Is it working?


D.J. Burba, DVM, Dipl. ACVS, Anna Guzman

Cribbing, also referred to a crib-biting is considered an acquired stereotypic oral behavior

that affects 4 to 5 % of the horse population. The exact cause of cribbing is not known.

However, multiple factors may contribute to the reason a horse wants to crib. These

include boredom, frustration, stress, diet, illness, neurological, and possibly hereditary.

Cribbing is considered for the most part a nuisance; however there are potential sequelae

that may develop as a result of cribbing. A cribbing horse may lose weight, perform poorly,

have frequent bouts of colic, and develop excessive wear of the upper incisors. These

sequelae develop as a result of the frequency of the cribbing behavior.

There have been various non-surgical as well as surgical procedures have been described with various success rates. Dr. Forssell

described the first surgical procedure to alleviate cribbing. This entailed resection of portion of the paired sternomandibularis,

omohyoideus, and sternothyrohyoideus muscles. Unfortunately this resulted in a poor cosmetic outcome which was unappealing

to owners. A modification to this technique was published in which resection of the ventral branch of the spinal accessory nerves

were performed in lieu of the sternomandibularis muscles.

Articles were published reporting the success of surgical procedures by numerous surgeons. The success rates were not

impressive in most of the reports. This may be due to a tendon-like fibrous union developing between the cut ends of the muscles.

It is hypothesized that this fibrous union can be reduced by a more rostral transection of the insertion of the sternohyoideous

and omohyoideus. A more rostral transection of the insertion of these muscles was reported in 2002 from LSU EHSP in which

a revision of the modified Forssell’s techniques was performed in 10 horses with 100% success. Since then, the procedure has

been performed on a total of 100 horses. The purpose of this study is to look at the success of the laser assisted revised modified

Forssell’s (LARMoF) surgical procedure to alleviate cribbing in horses.

Material and Methods

100 cases were reviewed from November 1994 to July 2010 and included in the study. Case records were reviewed and

information retrieved included duration of cribbing prior to surgery, gender, age, breed, use, and complications post-surgery if

any. A telephone questionnaire was performed as part of the study to gather information from the owner including the long-term

outcome of the surgery. The same surgical procedure previously described was used.

Results

Information from owners was obtained for 82 cases (Table 1). The number of horses that ceased cribbing was 68/82 (83%). Most

of the owners were pleased with the cosmetic outcome of the procedure (81/82, 99%). Of the horses (14/82) that surgery failed

to alleviate cribbing, the time post surgery cribbing behavior resumed ranged from 5 months to 3 years (Table 2). However all

reported less frequent episodes of the behavior. A few of the owners (5/85, 6%) felt that the horse’s performance was altered

negatively post-surgery. This included 2 racing Thoroughbreds, 2 cutting horses, and 1 Warmblood used for dressage.

62

Table 1. Information obtained from owners and records of cases in which LARMoF surgery was performed to alleviate

cribbing.

Table 2. Cases in which LARMoF surgery failed to alleviate cribbing.

SURGERY

63

SURGERY

Discussion

Results revealed that the LARMoF

technique is more successful in

alleviating cribbing then techniques

previously reported except one (table

3). Hakansson et al, reported an 89%

(92/103 horses) success; however

the Forssell’s technique was used

which caused a cosmetic defect in

neck which is not appealing to owner.

Of the surgeries that failed in our

study, nearly twice as many horses

had been cribbing more than 2 years

compared to cases that had cribbed

less than two years prior to surgery

( 9 vs 5). Complications did occur

in 17/82 (21%). This included post-

op hematoma/seroma formation,

prolonged drainage as defined as

more than 2 weeks post surgery,

post-operative infection, and partial

dehiscence. Of these, surgery failed

to alleviate cribbing in 10 horses. So it

appears complications also increase

the incidence that surgery will not alleviate cribbing. This may be due fibrous tissue formation contributing to the horse’s ability to

continue cribbing. It was interesting to note that box stall confinement was part of the daily life of all cases reported.

Conclusion

LARMoF is an effective technique for alleviating

cribbing in horses. Further work is needed to reduce the

complications associated with the surgery.

Table 3. Surgical success rate of previous reports of

alleviating cribbing in horses.

F=Forssell’s technique

MF=Modified Forssell’s technique

N=Neurectomy alone technique

Non-Profit Org.U.S. Postage

PAIDPermit No. 733

Baton Rouge, LA

Louisiana State UniversityBaton Rouge, Louisiana 70803

http://www.vetmed.lsu.edu

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