EDITORIAL BOARDL. LEON CAMPBELL, Editor-in-Chief

University ofIllinois, UrbanaL S. BARON, Editor (1969) L. JOE BERRY, Editor (1968)

Walter Reed Army Institute of Research, Washington, D.C. Bryn Mawr College, Bryn Mawr, Pa.

R. D. DeMOSS, Editor (1970)University ofIllinois, Urbana

R. L. Anderson (1969)Robert Austian (1969)W. Lane Baksdle (1969)S. S. Barkulis (1969)Alan W. BesP !Pr (1969)Robert W. Ber (1970)S. Gaylen Brdley (1969)Arnold F. Brodie (1970)Allan Campbell (1969)George B. Chapman (1969)V. P. Cirlo (1970)A. J. aark (1969)V. W. Cochm (1969)S. F. Coned (1969)0. D. Cox (1969)William J. Cromntie (1969)Roy Curds III (1969)A. L Demain (1969)John A. DeMoss (1969)Michael Douoroff (1969)Martin Dworkin (1970)Elis Englesberg (1969)Frnk B Engley, Jr. (1969)Stanley Falkow (1969)Wilam W. Ferguson (1969)Leonard Frank (1970)Martin Freundlich (1970)Herman Friedman (1969)

Lorraine Friedman (1968)Ruth E Gordon (1969)Joseph S. Gots (1969)Leonard Hayilick (1969)James G. Hirsch (1969)John G. Holt (1969)Pand Howard-Fladers (1969)Jerard Hurwitz (1969)John Ingrham (1970)Arthur G. Johusn (1969)Elliot Juni (1967)R. E. Kllio (1969)Carl Lann (1970)Maurice Landy (1970)H. S. Levinson (1969)H. C. Lichstel (1970)W. R. Lockhart (1969)Donld G. Lundgren (1968)Elzabeh McCoy (1969)George B. Macan (1969)Stanley Marcus (1970)Julius Mrmur (1968)A. G. Maw (1969)M. L. Morse (1969)Frederick C. Neidhardt (1969)Evelyn L. Oginsky (1969)Kenneth Paigen (1969)James T. Park (1969)Robert C. Parett (1969)

Harry D. Peck (1970)Mich J. Pelczar, Jr. (1969)a- J. Phaff (1969)Roy Repaske (1969)aF V. Rickenberg (1970)C. F. Robinow (1969)W. R. Romig (1969)M R. J. Salton (1969)Moselio Schechter (1970)Russell W. Schaedler (1969)W. R. Sistrom (1969)Louis DS. Smith (1968)Paul F. Smith (1970)Roger Y. Stanier (1970)ML P. Starr (1970)BE A. D. Stocker (1969)Benard S. Struss (1969)Curtis B. Thorne (1969)H. E. Umbarger (1970)Lewis W. Wannamaker (1969)Arthur Weissbach (1969)R. W. Wheat (1969)Helen R. Whiteley (1969)J. B. Wilson (1969)Meyer J. Wolin (1970)Wil A. Wood (1969)Barbara E. Wright (1968)Frank E. Young (1970)

ROBERT A. DAY, Managing Editor, 4715 Cordell Ave., Bethesda, Md. 20014

EX OFFICIOS. E. LURIA, President (1967-1968)

Massachusetts Institute of Technology, CambridgeDONALD E. SHAY, Secretary

University of Maryland, Baltimore

DENNIS W. WATSON, Vice-President (1967-1968)University ofMinnesota, Minneapolis

H. B. WOODRUFF, TreasurerMerck & Co., Inc., Rahway, N.J.

Published monthly by the American Society for Microbiology. Manufactured and ditibuted by Waverly Press, Inc., andThe Williams & Wilkins Co., at 428 E. Preston St., Baltimore, Md. 21202, U.S.A.

Subscription price $30.00 per year, single issue $4.00Second cLa postage paid at Baltimore, Md.

Made in the United States of America

AUTHOR INDEXAdhya, Sankar, 2065Agarwal, Manjul K., 1817

Bacon, Karen, 1883Bebki, R. M., 1837Bennett, R. W. 1875Berry, L. Joe, 1817Bhattacharyya, Pinailal, 2079Birnbaum, Jerome, 1846Bose, S. K., 2079Bradley, S. 0., 1994Breitman, T. R., 1890

Campbell Allan 2065Casman, t. P., 1'875Chou, James, 1934Clifton, C. E., 2077Condon, Sdamus, 1970Crum, Edward K, 2069

Deering, R A 1946del CampilloZmpbell, Alice, 2065DeMoss, J. A., 1896Dietz, Alma, 2022Dorsey, A. E., 1875Dubos, Rene 3., 1811Dunlap, K. R., 1919

Fortnagel, Peter, 1957Fredette, V., 2012Freese, Enst, 1957Frernan, Frank E., 1854, 1868

Gay, Frederick W., 2048Gooder, Harry, 2037Gullmar, B., 1924G5nther, ielge L., 2067

Halvorson, H. Orin, 2075Homer, Harry T., Jr., 2027Hutter, R., 1896

Ingraham, John L., 1970Issa, J. A., 1875

Johnson, E. M., 2018Jurtshuk, Peter, 2062

Kantor, George J., 1946Kasai, N., 1824Kayaanian, Gary, 2065

Lersten, Nels R., 2027Lesley, S. M., 1837Lichstein Herman C., 1846, 1930Loomis, William F., Jr., 2001

McFall, Elizabeth, 1982, 1989Magasanik, Boris, 2001Mastroeni, P., 2073Molin, N., 1924

Nacci, A., 2073Neu, Harold C., 1934Nowotny, Alois, 1824

Pai, Chik H., 1846, 1930Perry J. J., 1919Plantd C, 2012Pope, Le&Iocia M 2062Prusoff, William H., 2067

Rabinovitz, Marco, 1890Rn, Shmuel, 1807Renshaw, E., 1915Rocca, A. 2073Romano, Antonio H., 1951Rosenberg, Eugene 1883Rottem, Shlomo, 1A07Roy, A., 2012

Savage, Dwayne C., 1811Schell, Jane E.. 1951Siehr, Donald J., 2069Smith, David H 2071Smulson, Mark t 1890Snyder, Iri S., iA17Summers, Jesse W., 1908Swanson, Alice, 2075Szaniszlo, Paul J., 2037

Tewfik, Enayat M., 1994Thomson, A. J., 1915Tyler, Bonnie, 2001

Wegener Wamer S., 1951Wise, Join, 2075White, David C., 1854, 1868Wyss, Orville, 1908

5JOURNAL OF BACTERIOLOGY

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JOURNAL OF BACTERIOLOGY

JOURNAL OF BACTERIOLOGYThe Journal of Bacteriology, a publication of the American Society for Microbiology, is

devoted to the advancement and dissemination of fundamental knowledge concerning bacteriaand other microorganisms. The Journal is published monthly, and the twelve numbers are dividedinto two volumes (January-June and July-December). The subscription price is $30.00 per year, in-cluding Bacteriological Reviews. Single copies are $4.00. Members of the American Society for Mi-crobiology may receive the Journal as part of their dues.

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Correspondence. Submit manuscripts in duplicate(original and one carbon) to Robert A. Day, Manag-ing Editor, American Society for Microbiology, 4715Cordell Ave., Bethesda, Md. 20014.

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lows the Style Manualfor Biological Journals (2nd ed.,AIBS, 1964). Genetics symbols should essentially fol-low the recommendations of Demerec et al. (Genetics54:61, 1966). The Journal reserves the privilege ofediting manuscripts to make them conform with theadopted style. (Somewhat more complete "Instruc-tions to Authors" are printed in the January and Julyissues of the Journal.)Form of manuscript. Manuscripts should be typed

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6

Acknowledgment

The following have served as invited, special reviewers for the Journal during 1967, andtheir help is greatly appreciated.

P. ActorH. 1. AdlerJ. AdlerR. L. AirthJ. M. AkagiM. AlexanderB. AmesR. L. AnackerD. E. AtkinsonE. BalbinderJ. W. BartholomewD. C. BauerJ. V. BeckJ. R. BeckwithS. M. BeiserM. S. BergdollD. BillenA. BondiP. BonventreE. BorekH. W. BoyerJ. W. BraunT. R. BreitmanT. D. BrockV. BrooksH. P. BroquistG. M. BrownM. P. BryantR. E. BuchananJ. CairnsE. Canale-ParolaB. W. CatlinR. ClowesW. A. ClydeS. S. CohenR. L. ConnerH. E. ConradR. A. ConsigliT. M. CookM. J. CormierL. M. CorwinR. N. CostilowJ. P. CraigI. P. CrawfordR. L. CrowellS. DagleyR. H. DeibelJ. DeLeyC. C. DelwicheJ. DittmerW. J. DobrogoszB. P. DoctorR. N. DoetschR. DoiM. I. DolinH. C. DouglasJ. D. Duerksen

J. M. EisenstadtP. D. EllnerH. L. EnnisJ. C. EnsignW. H. EwingC. FishelM. FishmanM. FlavinS. B. FormalH. FreedmanR. G. FreterD. FriedmanH. H. GadebuschE. T. GaudyP. GemskiP. GerhardtJ. C. GerhartH. GestD. T. GibsonW. F. GoebelH. GooderL. GoriniJ. W. GormanE. D. GrayJ. B. GroganS. R. GrossL. GrossmanB. M. GuirardI. C. GunsalusH. GutzF. L. HaasR. F. HaffL. P. HagerF. E. HahnH. HalvorsonP. C. HanawaltD. H. HeilmanD. R. HelinskiL. HeppelC. W. HesseltineR. D. HigginbothamD. S. HoareG. L. HobbyT. HoffmannP. K. HolmesD. H. HopwoodR. E. HungateM. HuppertC. K. HustonD. J. HutchisonS. H. HutnerJ. L. IngrahamV. N. IyerJ. M. JayE. M. JohnsonR. C. Johnson

S. KaplanF. A. KapralE. KatzB. T. KaufmanF. T. KenneyY. B. KimH. P. KleinG. S. KobayashiA. L. KochW. A. KonetzkaF. P. KoontzL. 0. KrampitzH. E. KubitschekC. G. KurlandJ. 0. Lampen0. E. LandmanC. E. LankfordK. G. LarkJ. H. LawE. R. LeadbetterH. LechevalierI. R. LehmanL. LeiveG. LesterH. B. LevineC. LevinthalJ. A. LippincottS. E. LuriaW. K. MaasR. H. McBeeM. McCartyW. G. McCulloughJ. C. MacdonaldB. A. McFaddenE. McFallR. A. McLeanR. A. MacLeodM. F. MalletteA. A. MarucciW. H. MatchettD. B. MelvilleR. L. MetzenbergN. G. MillerW. S. MillerS. MillsG. J. MiragliaA. G. MoatW. E. C. MooreR. Y. MoritaH. J. MorowitzS. MorseR. K. MortimerV. MosesJ. S. MoulderR. G. E. MurrayL. H. Muschel

Q. N. MyrvikE. M. NeptuneE. W. NesterF. C. NeuhausJ. NewtonC. F. NivenM. NomuraG. D. NovelliA. NovickR. P. NovickA. NowotnyJ. 0. O'NeillC. H. OppenheimerZ. J. OrdalM. J. OsbornE. I. OttolenghiJ. F. PaganoR. PakulaA. PardeeD. ParetskyL. W. ParksA. PeterkofskyN. PfennigR. E. PieroniL. PineA. J. PittardA. Pomales-LebronM. RachmelerJ. R. RaperK. B. RaperW. E. RazzellJ. RobinsonL. J. RodeS. D. RodenbergF. RothmanR. RowndG. RushizskyW. J. RutterC. L. San ClementeM. SanterG. F. SaundersA. J. SbarraE. J. SchantzH. W. ScherpC. SchildkrautH. G. SchlegelF. SchlenkD. SchlessingerG. J. SchroepferJ. H. SchwabW. SegalJ. SetlowR. SetlowS. K. ShapiroP. D. ShawT. Shiota

G. D. ShockmanM. S. ShorbC. W. ShusterL. ShusterS. J. SilvermanM. SingerH. D. SladeR. SlepeckyS. S. SocranskyJ. R. SokatchM. L. SpeckJ. K. SpitznagelJ. SpizizenD. B. SprinsonE. R. StadtmanT. C. Stadtman0. H. StalheimA. B. StavitskyJ. L. StokesA. J. L. StraussN. SueokaH. SugiyamaS. R. SuskindA. S. SussmanP. S. SypherdW. A. TaberH. TaborM. TagerG. TomkinsM. E. TourtellotteL. UngerM. F. UtterJ. A. ValuJ. T. WachsmanG. WarrenD. W. WatsonM. M. WeberE. D. WeinbergL. WeinsteinE. WeissH. WeissbachS. M. WeissmanN. E. WelkerH. J. WhitfieldR. P. WilliamsP. W. WilsonC. L. WittenbergerJ. A. WohlhieterR. S. WolfeM. J. WolinJ. WoodC. YanofskyH. YoshikawaG. P. YoumansL. N. ZimmermanL. Zubrzycki

JOURNAL OF BACTERIOLOGY, Dec. 1967, p. 2081-2084Copyright © 1967 American Society for Microbiology

AUTHOR INDEX

Achey, P. M., 1538Adams, James N., 650Adelberg, Edward A., 1623Adhya, Sankar, 2065Agarwal, Manjul K., 1817Ail, Samuel J., 1075, 1560Akiba, Tomoichiro, 687Alberghina, F. A. M., 630Alexander, M., 624Allnoch, Hedi, 1251Amos, Harold, 232Anacker, R. L., 260, 1736Andre-Schwartz, Janine, 232Apirion, David, 1275Applegarth, D. A., 1787Arbuthnott, John P., 1170Arias, L., 1783Avers, Charlotte J., 1225, 1236

Baarda, J. R., 53Bacon, Karen, 1883Baginsky, Marietta L., 1034Baker, Bruce, 388Barclay, William R., 1736Barker, E. R., 258Barker, L. F., 403Barksdale, Lane, 1565Barkulis, S. S., 963Bauchop, T., 171Beck, Jay V., 1052Becker, George E., 48Beckett, K. M. K., 1797Beers, R. J., 517Beesley, E. D., 19Beggs, William H., 793Behki, R. M., 1837Bennett, E. O., 344Bennett, R. W., 1875Benson, A. A., 1679Bernheimer, Alan W., 1170Berry, L. Joe, 1817Berst, M., 1366Bhattacharyya, Pinakilal, 2079Bhuyan, Bijoy K., 576Billen, D., 1538Birnbaum, Jerome, 1846Black, S. H., 759Bleiweis, Arnold S., 1381, 1560Blobel, H., 780Boder, George B., 1257Bodmer, Walter F., 1277Boltralik, J. J., 963Bonventre, Peter F., 306Boone, Clarence J., 875Bose, S. K., 2079Botsford, J. L., 966Bott, Kenneth F., 562Bowers, W. D., Jr., 482Bowman, James E., 544Boyle, James V., 1664

Bozeman, F. M., 1334Bozoian, G., 1787Bradley, S. G., 1994Brame, Joe, 782Brehmer, Werner, 1736Breitman, T. R., 1890Brenner, Don J., 486Brockman, Ellis R., 1253Brown, June M., 1287Brownell, George H., 650Brownell, Sandra, 597Brubaker, R. R., 19, 544Bryson, Vernon, 38Buckmire, F. L. A., 1764Budman, Daniel R., 1546Bulger, Roger J., 1244Bulger, Ruth Ellen, 1244Bulmer, G. S., 1475, 1480Burrell, Robert, 914

Caldwell, J., 791Campbell, Allan, 2065Campbell, L. Leon, 1124, 1131Campbell, P. A., 791Canale-Parola, E., 984Cannefax, George R., 1359Carbonell, Luis M., 213Carlisle, Sharon A., 1247Carlton, Bruce C., 660Carr, Howard S., 1025Carson, Paul E., 544Cashel, Michael, 522Casman, E. P., 1875Caster, John H., 1804Catlin, B. Wesley, 719Chai, Ning-Chun, 415Cho, K. Y., 196Chou, James, 1934Ciegler, A., 787Clark-Walker, G. D., 1648Clifton, C. E., 2077Clowes, R. C., 365Coffey, John J., 1638Cohen, Irun R., 141Cohen, Seymour S., 1684Cohen-Bazire, Germaine, 458Cole, B. C., 1451Colvin, J. Ross, 202Conant, Norman F., 1400Condon, S6amus, 1970Converse, J. L., 1394Cook, Thomas M., 1664Costerton, J. W., 1764Costilow, Ralph N., 759Cowman, R. A., 942Craddock, John G., 1728Cromartie, William J., 1728Crouch, Dorothy, 1793Crum, Edward H., 2069Cuppels, D., 1797

2081

Curtiss, Roy, III, 490

Dainko, J. L., 1509Dale, Beverly, 778Deering, R. A., 1946Dehority, B. A., 537Deibel, R. H., 291, 297del Campillo-Campbell, Alice,

2065DeLey, J., 116Demain, Arnold L., 66, 323DeMoss, J. A., 1896de Repentigny, J., 13de Uruburu, F., 241Diaz, Ramon, 499Dickerman, Herbert, 1609Dietz, Alma, 2022Dobbs, James P., 600, 609Domer, Judith E., 466Dorsey, A. E., 1875Dowell, V. R., Jr., 1300Doy, Coline H., 196Dubos, Rene J., 1811Duncan, John F., 1262Dunlap, K. R., 1919Durkee, Thomas L., 1757

Edwards, Mercedes R., 766Elbein, Alan D., 1520Elberg, Sanford S., 1793Elisberg, B. L., 1334Ellar, D. J., 1189, 1778Ellner, Cornelia J., 1025Ensign, Jerald C., 734, 741Esterly, J. R., 1394

Faber, J. E., 1334Farmer, Silas, 272Farshtchi, Davood, 255Federman, Micheline, 1236Fedorko, Joseph, 1251Feiner, Rose R., 1270Fernelius, Albert L., 552Finegold, Sydney M., 1443Fishel, Charles W., 804Fleming, R. W., 855Foley, G. E., 1797Ford, Scott, 928Forrest, W. W., 1459Forsberg, Cecil, 1764Fortnagel, Peter, 1957Frank, Hilmer A., 506Frankel, Ruth W., 32Fredette, V., 1437, 2012Freer, John H., 441, 1170Freese, Ernst, 522, 1957Freifelder, David, 396Frerman, Frank E., 1854, 1868Friedman, Lorraine, 928Frischer, Henri, 544

Vol. 94, No. 6Printed in U.S.A.

AUTHOR INDEX

Frommes, S. P., 224Furmanski, Phillip, 1075

Gale, Nord L., 1052Galton, Michael, 590Garibaldi, J. A., 1296Gay, Frederick, W., 2048Gelpi, E., 333Georg, Lucille K., 1287Gerber, M. J., 1672Gerencser, Mary Ann, 109Ghiorse, William C., 766Gleason, Frank H., 1785Golightly, L., 1451Gooder, Harry, 2037Goodgal, Sol H., 1802Goodman, N. S., 485Goodman, Richard E., 1154Gorbach, Sherwood, 378, 383Gordee, Robert S., 6Gordon, Dwight F., Jr., 1281Gordon, Morris A., 766Goren, Mayer B., 1258Goss, William A., 1664Goto, Sachio, 751Gottfried, R. J., 485Gottlieb, David, 844Greenberg, G. Robert, 778Greiff, Donald, 272Gross, Julian D., 1603Grover, A. A., 832Guest, W. J., 1388Gullmar, B., 1924Gunn, C. M., 1475Gunther, Helge L., 2067

Hall, Wendell H., 92Halvorson, H. Orin, 2075Hamilton, James G., 466Handley, Joan, 263Hankin, H., 963Hanson, Albert W., 1359Haque, Riaz-ul, 1327Harada, Noriko E., 1443Harkin, James C., 466Harold, F. M., 53Harris, Virginia G., 27Hartwell, Leland H., 1697Hashimoto, Hajime, 1166Heberlein, Gary T., 116, 1470Helinski, Donald R., 691, 700Hendlin, David, 66Herschman, Harvey R., 691, 700Hernandez, Eovaldo, 991, 996Hewitt, R. R., 1538Heymann, H., 963Higgins, Michael L., 495Hildebrand, D. C., 1785Hill, Caroline, 677Hoare, D. S., 782Hoare, S. Louise, 782Holland, I. B., 677Holmes, P. K., 434Homma, J. Yusuru, 751

Horner, Harry T., Jr., 2027Horodniceanu, Thea, 1268Horvath, Istvan, 850Hoyer, Bill H., 486Hubbard, Jerry S., 1007, 1016Hubersak, C., 1795Humphries, J. W., 1334Huppert, Milton, 924Hutchison, Dorris J., 1264Hutchison, H. Terry, 1697Hutter, R., 1896Hyde, James M., 245

Ide, Misao, 317Igarasi, Seizi, 1551Imada, Akira, 1551Imsande, John, 1672Ingraham, John L., 157, 1970Inoue, Yukio, 844Inselburg, Joseph, 1266Issa, J. A., 1875Itakawa, Hiraku, 1800

Jackson, M., 323Jagger, John, 165Jansen, Miekie, 1684Janssen, W. A., 19Jarvis, Francis G., 1406, 1411Jenne, John W., 793Jensen, Roy A., 1582Johnson, Byron F., 192Johnson, Charles E., 306Johnson, Emmett J., 409Johnson, E. M., 2018Johnson, Marvin J., 101, 991,

996, 1502Johnson, Robert W., 1257Johnson, Russell C., 27Jones, Lois M., 499Jones, William I., Jr., 600, 609Joys, Terence M., 32Jurtshuk, Peter, 2062

Kahn, A. J., 1272Kang, Soosang, 87Kantor, George J., 1946Kappy, Michael S., 1629Kasai, N., 1824Katz, Sol, 1251Kaufman, Leo, 798Kawakami, Masaya, 902Kayajanian, Gary, 2065Keller, Kenneth F., 804Kelly, Richard T., 272Kessel, M., 1464Kilgore, Wendell W., 666Kim, H. K., 832Kim, Yoon Berm, 1320Kind, P., 791Kingdon, Henry S., 949Kingsbury, David T., 557, 870,

1262Klainer, Albert S., 378, 383Klein, Frederick, 552, 600, 609

Klein, Harold P., 61, 475Koffron, Kara L., 1516Kogoma, Tokio, 1715Kowaluk, Patricia, 537Krause, Richard M., 1381Kress, Yvonne, 1216Krieg, Noel R., 1431Krulwich, Terry A., 734, 741Kuhn, Anneli, 232Kuo, M.-J., 624Kupfer, Dorothy G., 984Kurashige, Satonori, 902, 907Kusaka, Iwao, 884

Lackman, D. B., 260Laird, Charles D., 1277Lambert, Frank W., Jr., 1287Landau, Joseph W., 919Landay, Marshall E., 1400Lapa, Edward W., 766Lapthisophon, T., 1538Lark, Karl G., 415Larkin, J. M., 889Lascelles, J., 1648Lau, H. S., 1722Lazarus, H., 179 7Lazzarini, Robert A., 125Leaffer, Morris A., 61Lee, Chin K., 530Lees, H., 1484LeJohn, H. B., 1484Leong, Daniel, 499Lersten, Nels R., 2027Lesley, S. M., 1837Levine, H. B., 924Levinson, Hillel S., 434, 441Levinthal, Cyrus, 176Lewis, V. J., 1300Lichstein, Herman C., 92, 1846,

1930Lieberman, Michael M., 1492,

1497Lillehoj, E. B., 787Lincoln, Ralph E., 552, 600, 609Lindorfer, Robert K., 224, 300,

1313Lindstrom, E. S., 784, 860Lippincott, James A., 1470Lockhart, W. R., 826Logan, Gerald F., Jr., 934London, Jack, 458, 972Loomis, William F., Jr., 2001Loprieno, Nicola, 1162Lowe, Edwin P., 1394, 1400Lowry, P. N., 274Lowry, Robert J., 1757Luderitz, O., 1366Lund, Pauline G., 279Lundgren, D. G., 1189, 1778Luria, S. E., 1112Lyon, Richard H., 92

Maas, Werner K., 712McCarthy, Brian J., 1066

2082 J. BACrERIOL.

AUTHOR INDEX

McCarthy, Charlotte, 131McClary, D. O., 482McClung, Norvel M., 255McFall, Elizabeth, 1982, 1989MacFarren, Adelaide C., 365McGinnis, James, 493McLaughlin, David, 798MacLeod, Robert A.. 1764McRipley, R. J., 1417, 1425Magasanik, Boris, 2001Maheswaran, S. K., 224, 300

1313Mahlandt, Bill G., 600, 609Maisch, W. F., 1088Mallette, M. F., 283Manchee, Richard J., 1781Manire, G. P., 1178, 1184Manocha, M. S., 202Manohar, M., 224Mao, James C.-H., 80Maragoudakis, Michael E., 512,

1060Markovitz, Alvin, 87, 388, 1492,

1497Marquez, Joseph A., 789Marshall, Rosemarie, 517, 812Martin, Donald G., 1406, 1411Martin, Malcolm A., 486Mastroeni, P., 2073Mathieu, L. G., 13Matula, Tibor I., 1764Mayeux, Jerry V., 409Mednis, Aiga, 1025Mehta, Bipin M., 1264Mendelson, Neil H., 1603Mercer, E. H., 196Merritt, Katherine, 590Mesenko, Marianne T., 1406Metzenberg, Robert L., 1629Mickelson, M. N., 184Miller, Kent D., 580Miller, Lawrence G., 1443Miller, M. W., 258Milner, Kelsey C., 1411Mise, Katsutoshi, 1616Mitruka, Brij M., 759Mitsuhashi, Susumu, 902, 907,

1166Molin, N., 1924Monreal, J., 241Moore, Richard L., 1066Moriyama, Takafumi, 1565Morris, David R., 1516Morse, Guy E., 1247Mortimer, Edward A., Jr., 268Moss, C. Wayne, 1300Moulder, James W., 867Mudd, Stuart, 751Murphy, Richard A., 1327Murphy, Samuel G., 580, 586

Nacci, A., 2073Nagel de Zwaig, Rosa, 1112Nakaya, Rintaro, 1616

Nasser, DeLill S., 1582, 1706Nelson, John B., 1340Nester, E. W., 131, 1582, 1706Neu, Harold C., 1934Nevin, T. A., 1388Newcomer, Victor D., 919Niederman, R. A., 1002Niederpruem, Donald J., 1594Nomura, M., 1093Nowlan, Sandra Simpson, 291,

297Nowotny, Alois, 1824

O'Connor, J. E., 815Oden, Edwin M., 789Ogg, James E., 488Ogur, M., 482Ohanian, S. H., 1346, 1728Okabayashi, Tadashi, 317Okamoto, Suyehiko, 1616Ordal, Z. John, 530, 1082Oro, J., 333, 344, 349Ortega, Manuel V., 707Osawa, Nobutaka, 902, 907

Pai, Chik H., 1846, 1930Paigen, Kenneth, 493Pakula, Roman, 75Paquette, G., 1437Parada, Jose L., 707Pardee, Arthur B., 1546Paretsky, D., 263Parks, L. W., 966, 1531Patt, J. K., 403Pepper, R. E., 759Perrine, Theodore D., 1406Perry, Jerome J., 1249, 1919Person, Stanley, 165Peterson, E. J., 924Peyru, Graciela M., 712Phillips, Stephen L., 165Pickens, E. G., 260Pine, Leo, 875Pitt, J. I., 258Pittard, J., 1279, 1656Plante, C., 2012Pooley, Harold M., 1525Pope, L. M., 1206, 2062Postel, Edith H., 1802Pruess, David L., 1502Prusoff, William H., 2067Pulliam, James D., 1394

Quinn, L. Y., 812Quinn, R. W., 274

Rabinovitz, Marco, 1890Raina, Aarne, 1684Raj, H. D., 615Raper, Kenneth B., 1143, 1150Razin, Shmuel, 359, 1807Reeves, Henry C., 1075, 1560Remsen, Charles C., 422

Renshaw, E., 1915Reusser, Fritz, 576, 1040Ribi, Edgar, 1736Richter, G. W., 1216Rittenberg, B., 1648Roberts, David B., 958Rocca, A., 2073Rode, L. J., 1206Rodwell, Victor W., 1034Rogers, Palmer, 92Rogoff, M. H., 485Rolls, James P., 784, 860Romano, Antonio H., 1951Rosenbaum, Nancy, 1497Rosenberg, Eugene, 1883Rosenberger, R. F., 1464Rosenkranz, Herbert S., 1025Rosner, J. L., 1623Rothschild, Henry, 1800Rottem, Shlomo, 359, 1807Roy, A., 2012Ruschmann, E., 1366

Sacks, L. E., 1789Saito, Kazuko, 907San Clemente, C. L., 821Sands, David C., 1785Sans, M. D., 1475, 1480Santangelo, Edith, 125Sasarman, A., 1268Savage, Dwayne C., 1811Sbarra, A. J., 149, 1417, 1425Schantz, Edward J., 277Schekter, M. A., 1300Schell, Jane E., 1951Scherago, M., 1722Schlenk, F., 1509Schlesinger, Sondra, 327Schlessinger, David, 1275Schubert, Joseph H., 798Schwab, John H., 1346, 1728Scott, H. W., 537Selvaraj, R. J., 149Sengers, R. C. A., 1791Shah, D. B., 780Shapiro, Stanley K., 1136, 1531Shaw, Maxwell K., 157Shirai, A., 1334Shively, J. M., 1679Shockman, Gerald D., 1525Shorb, Mary S., 279Siegel, Eli C., 38Siehr, Donald J., 2069Silverman, Melvin P., 1046Simpson, Patrick J., 6Sirotnak, Francis M., 1264Slack, John M., 109Slein, Milton W., 934Slepecky, R. A., 1189Smith, Arnold J., 972Smith, D. W., 832Smith, David H., 2071Smith, K. L., 300Smulson, Mark E., 1890

VOL. 94, 1967 2083

AUTHOR INDEX

Snow, Richard, 571Snyder, E. M., 1394Snyder, I. S., 815, 1817Socransky, S. S., 1795Sogin, Stephen J., 1082Somerville, Ronald L., 1798Sonea, S., 13Speck, M. L., 942Spence, Kemet D., 1136, 1531Spero, Leonard, 277Spotts, Charles R., 1154Stadtman, E. R., 949, 1007, 1016Stallions, D. R., 490Stanier, Roger Y., 972Stefanye, David, 277Stokes, J. L., 889Strassman, Murray, 512Strominger, Jack L., 734, 741Stueckemann, Johanna, 263Stuka, Andrew J., 914Stutzenberger, Fred J., 821Summers, Jesse W., 1908Sun, S. H., 924Surgalla, M. J., 19Suskind, S. R., 630, 1800Sussman, Alfred S., 1306, 1757Suzuki, Hiromi, 666Suzuki, Kaname, 1166Suzuki, Yoshiaki, 1616Swaisgood, H. E., 942Swanson, Alice, 2075Sydiskis, Robert J., 1492Szaniszlo, Paul J., 2037Szentirmai, A., 850

Tabatabai, L., 1805Takayasu, Hisao, 687Tamura, Akira, 1178, 1184Taylor, Robert L., 1Taylor, Robert T., 1609Taylor-Robinson, David, 1781Terawaki, Yoshiro, 687

Terry, Robert T., 798Tewfik, Enayat M., 1994Thayer, Donald W., 488Thomson, A. J., 1915Thompson, J. Stuart, 1525Tijtgat, R., 116Tipper, Donald J., 734, 741Toama, Mohamed A., 1143,

1150Tomelty, Joseph P., 1431Tornabene, T. G., 333, 344, 349Torriani, Annamarie, 176Trenner, N. R., 323Trentini, William C., 1260Trice, Karen L., 896Truper, Hans G., 1255Turgeon, S., 13Tyler, Bonnie, 2001

Uehara, Minoru, 506Ueno, Yoshio, 786

Valois, Frederica W., 422van Caeseele, L., 1484Van Zandt, Janice, 245Vastine, Ellen L., 268Vender, Joyce, 867Villanueva, J. R., 241Volkmann, Carol M., 61, 475

Wachsman, J. T., 1088Wagman, Gerald H., 789Wailes, K. A., 855Walker, E. M., 1656Walker, H. W., 1805Walker, Jerry S., 552, 600, 609Walkinshaw, Charles H., 245Wallace, B. J., 1279Wang, C. J. K., 597Ward, J. R., 1451Watson, Dennis W., 1320Watson, Stanley W., 422

Wax, Richard, 522Wegener, Warner S., 1075, 1951Weibel, Joseph, 475Weinstein, Louis, 378, 383Weinstein, Marvin J., 789Weissbach: Herbert, 1609Welker, N. E., 1124, 1131Wells, J. Scott, Jr., 1431Werth, J. M., 1272Wessels, Joseph G. H., 1594Westphal, O., 1366Wheat, R. W., 1366, 1400White, David C., 1854, 1868Wiegeshaus, E. H., 832Willett, Norman P., 1247Williams, Beverly, 493Williams, F. D., 855Wilson, Gary A., 562Wilson, J. B., 499Winshell, Elaine B., 1025Wise, John, 2075Witten, C., 1093Wolin, M. J., 1002Wooley, Susan, 1306Wu, William G., 896Wyss, Orville, 1903

Yamamoto, T., 1746Yanagita, Tomomichi, 1715Yarmolinsky, Michael B., 1623Yentsch, Charles S., 1255Yolton, Diane P., 1206Yoshimoto, Akihiro, 317Yotis, William W., 1353Youmans, Anne S., 836Youmans, Guy P., 836Young, Frank E., 1381, 1783Yphantis, D. A., 1509Yu, Shih-an, 1306

Zsandanyi, J., 850

2084 J. BACTERIOL.

JOURNAL OF BACTERIOLOGY, Dec. 1967, p. 2085-2093Copyright © 1967 American Society for Microbiology

SUBJECT INDEX

Acetobacter suboxydans, condensing enzyme re-action in, 512

acetylcholinesterase inhibition by botulinum toxin,812

Actinomyces, morphological forms of, 875Actinomyces israelii, identification, 1287Actinomyces naeslundii, identification, 1287Actinomyces propionicus, characterization, 109Actinoplanaceae, cell wall composition, 2037Actinoplanes, sporangiospore release by, 495adenine, germinant for Bacillus macerans spores, 1789adenine, use in nucleic acid labeling, 1262adenosine cyclic 3', 5'-phosphate, formation in

Brevibacterium liquefaciens, 317adenosine triphosphate, production by microor-

ganisms, 991, 996adenosine triphosphate-dependent carbon dioxide

fixation, 409adenosylhomocysteine, inhibition of Saccharomyces

cerevisiae by, 1136adenosylmethionine, inhibition of Saccharomyces

cerevisiae by, 1136S-adenosylmethionine, role in methionine biosyn-

thesis in yeast, 966adjuvants, effects on mycobacterial ribosomal frac-

tion, 836adrenergic blockade in Bordetella pertussis-vaccinated

mice, 804aeciospores of Cronartium fusiforme, 245Aerobacter cloacae, anaerobic growth of, 991aerobic growth at low oxygen concentrations, 101aerobic growth of microorganisms, 996aerobic metabolism of Streptococcus agalactiae, 184aflatoxin B,, effect on deoxyribonucleic acid syn-

thesis, 787aging of Pseudomonas aeruginosa, 2077Agrobacterium, deoxyribonucleic acid of, 116Agrobacterium tumefaciens, effect of mitomycin C

on, 1470airborne tuberculosis, low-temperature inoculum

for, 832L-alanine, production by Micrococcus sodonensis,

1249L-alanine, role in spore germination, 522algae, blue-green, autotrophy in, 972alkaline phosphatase, synthesis in Escherichia coi, 87alkaline phosphatase of Staphylococcus aureus, 780amino acid composition of type A botulinum toxin,

277amino acid synthesis in Bacillus subtilis, 1706ca-amylase of Bacillus amyloliquefaciens, 1131anaerobes, oxygen sensitivity of, 2012anaerobic growth of microorganisms, 991anthraquinoid pigments, synthesis in Penicillium

islandicum, 786anthrax bacilli, host distribution, 600anthrax infection, antibiotic treatment, 609anthrax pathogenesis, test animals for studying, 552antibiotic components of gentamicin complex, 789

antibiotic susceptibility, use in classifying bacilli,1443

antibiotic treatment of anthrax infection, 609antibiotic U-24,544, mode of action of, 1040antibiotics, inhibitory activity of, 576antibody response to Mycobacterium tuberculosis, 815antibody to Salmonella enteritidis, 902, 907antifungal action of flavensomycin, 844antigen, capsular, of Klebsiella pneumoniae, 896antigen, Vi, properties of, 1406, 1411antigen-antibody combinations, electron micros-

copy of, 751antigen from streptococcal cell walls, 1381antigenic derivatives of tetanus toxin, 580antigenicity of Trichophyton rubrum, 914antigens, serological response of monkeys to, 1

antigens, somatic, in Salmonella, 2018antigens, streptococcal, in vivo degradation, 1346antigens of Bacillus megaterium, 2073antigens of Brucella, 499antigens of Neisseria gonorrhoeae, 141arginine, induction of luciferase synthesis by, 1638arginine biosynthesis by Streptococcus bovis, 1002arthritis, effect of streptococcal cell walls on, 1728Arthrobacter crystallopoietes, sphere-rod morpho-

genesis in, 734, 741arthrospore vaccine against coccidioidomycosis, 1394ascospore numbers in Metschnikowia, 258aspergilli, pathogenicity for mice, 928Aspergillus flavus, oxidation of #3-nitropropionic

acid by, 48Aspergillus glaucus, formation of a-hydroxyglutaric

acid by, 1560Aspergillus nidulans, nuclear replication in hyphae of,

1464autolysin, localization in Streptococcus faecalis, 1525auxotroph of Escherichia coli, growth of, 1609autotrophy in blue-green algae, 972autotrophy in thiobacilli, 972avirulent Clostridium perfringens strains, 14376-azathymine, effect on deoxyribonucleic acid, 2067Azotobacter vinelandii, microtubule in, 2062

bacilli, chloramphenicol-acetylating enzymes in,1616

bacilli, classification by antibiotic susceptibility,1443

Bacillus, taxonomy of psychrophilic strains, 889Bacillus amyloliquefaciens, classification of, 1124, 1131Bacillus anthracis, host distribution, 600Bacillus anthracis, temperature response to, 552Bacillus cereus, chromosome incorporation during

sporulation, 1715Bacillus cereus, dipicolinic acid-less mutants, 2075Bacillus cereus, lethal toxin of, 306Bacillus cereus, penicillinase formation in, 1672Bacillus cereus spores, protein synthesis in, 177Bacillus coagulans, chemically defined media for, 517Bacillus licheniformis, glutamine synthetase from,

1007, 1016

2085

Vol. 94, No. 6Printed in U.S.A.

SUBJECT INDEX

Bacillus macerans spores, germinant for, 1789Bacillus megaterium, 1088Bacillus megaterium, antigens of, 2073Bacillus megaterium, fine structure of, 1189Bacillus megaterium, microcycle sporogenesis of,

434, 441Bacillus megaterium protoplasts, division of, 884Bacillus polymyxa, biotin-deficient growth of, 1908Bacillus popilliae spores, comparison with other

forms, 759Bacillus stearothermophilus, deoxyribonucleic acid of,

1805Bacillus subtilis, a-amylase of, 1131Bacillus subtilis, aromatic amino acids in, 1706Bacillus subtilis, 5-bromouracil utilization by, 1277Bacillus subtilis, flagellation of, 32Bacillus subtilis, macromolecular synthesis in, 131Bacillus subtilis, mycobacillin production, 2079Bacillus subtilis, temperature-sensitive mutant, 1603Bacillus subtilis, tryptophan biosynthesis in, 660Bacillus subtilis cell walls, N-acyl-galactosamine in,

1783Bacillus subtilis cell walls, substructure of, 1778Bacillus subtilis mutant, phosphohydrolases of, 66Bacillus subtilis spores, effect of L-alanine on, 522Bacillus subtilis spores, ribonucleic acid of, 125Bacillus subtilis transformation system, 562Bacillus thuringiensis, separation of spores and

crystals, 485biotin, biosynthesis of, 1930biotin, effect on growth of Bacillus polymyxa, 1908biotin, effect on synthesis of anthraquinoid pigments,

786biotin, synthesis in microorganisms, 1846biotin-deficient Micrococcus sodonensis, 1249biotin-requiring Escherichia coli mutants, 2065Blastomyces antigens, response of monkeys to, 1Blastomyces dermatitidis, budding process of, 213Bordetella bronchiseptica, fine structure of, 1216Bordetella pertussis vaccine, response of mice to, 804Borrelia hispanica, immune tolerance to, 1359botulinum toxin, acetylcholinesterase inhibition by

812botulinum toxin, amino acid composition of, 277bovine serum, nephelometric assay of coagulase

inhibitors in, 821branch-point enzyme in microorganisms, 1582Brevibacterium liquefaciens, formation of adenosine

cyclie 3', 5'-phosphate in, 3175-bromouracil utilization by Bacillus subtilis, 1277Brucella antigens for indirect hemagglutination test,

499Brucella melitensis, response to erythritol, 1793buffer for studies at pH 7, 283

Calvin cycle in Thiobacillus ferrooxidans, 1052Candida albicans infections, effect of irradiation on, 6Candida utilis, effect of oxygen concentration on, 101Candida utilis, effect of proteins on, 1509capsular antigen of Klebsiella pneumoniae, 896capsular polysaccharide, production by spreptococcal

L forms, 268capsule of Cryptococcus neoformans, 766carbohydrate metabolism in streptomycetes, 1520

carbon tetrachloride, influence on liver enzymes,1817

carbon utilization by sporulation mutants, 1957catabolism of glucose by meningopneumonitis

agent, 867catabolite repression in the gal operon, 493catabolite repression in Thiobacillus novellus, 1484cation levels, effect on a-hemolysin, 1791cell division of Bacillus megaterium, 1189cell-free extract of Staphylococcus aureus, protein

synthesis in, 80cell wall analyses of Actinomyces, 875cell wall composition in Actinoplanaceae, 2037cell wall during budding of Blastomyces dermatitidis,

213cell wall during budding of Paracoccidioides brasilien-

sis, 213cell-wall glucan degrading enzyme, role in mating,

1594cell walls, action of autolysin on, 1525cell walls, streptococcal, biosynthesis of, 963cell walls of Arthrobacter crystallopoietes, 734, 741cell walls of Bacillus subtilis, 1778, 1783cell walls of Histoplasma capsulatum, 466cell walls of meningopneumonitis organisms, 1178,

1184cell walls of Neurospora crassa, 202cell walls of streptococci, antigen from, 1381cell walls of Thiobacillus thiooxidans, 2069cellular slime mold, microcysts of, 1143, 1150chemically defined media for Bacillus coagulans, 517chemotaxis in Escherichia coli, 855chitin, in Penicillium cell wall, 1787chloramphenicol, effect on chemotaxis in Escherichia

coli, 855chloramphenicol-acetylating enzymes in bacilli, 1616Chromobacterium, deoxyribonucleic acid of, 116chromosome incorporation into Bacillus cereus

sporangium, 1715citric acid, biosynthesis in Acetobacter suboxydans,

512Citrobacterfreundii, Vi antigen from, 1411clear spot formation on Pseudomonas aeruginosa

cultures, 1251Clostridium bifermentans spores, appendages of,

1206Clostridium botulinum, effect of nutrients on, 1924Clostridium botulinum, physiological properties of,

1924Clostridium perfringens, avirulent strains, 1437Clostridium thermosaccharolyticum, glucose metabo-

lism in, 530coagulase, nephelometric assay of, 821coagulase activity in Pasteurella pestis, 19Coccidioides antigens, response of monkeys to, 1Coccidioides immitis, resistance of mice to, 924Coccidioides immitis, serology of, 1400coccidioidomycosis, cytolytic plasma factor in, 919coccidioidomycosis, vaccine against, 1394colicin E susceptibility in Escherichia coli, 677colicin factors in Escherichia coli, 365colicin induction in Escherichia coli, 691colicinogenic factors, effect of Rec- mutations on, 700colicins, interaction with bacterial cells, 1093, 1112

2086 J. BACTERIOL.

SUBJECT INDEX

competence, development in Bacillus subtilis trans-formation system, 562

competence-provoking factor of streptococci, 75complement, role in immune inactivation of Myco-

plasma gallisepticum, 403complement requirement for spirochetolysis, 1388conjugation, 3-galactosidase transfer in, 1623conjugation in Escherichia coli, 396, 490, 1656contraceptives, biological properties of, 1353Corynebacterium acnes, characterization, 1300Corynebacterium diphtheriae, neuraminidase of, 1565Corynebacterium glutamicum, nutritional require-

ments, 323cotton rat, infection with Rickettsia rickettsii, 1334Coxiella burnietii, ultrastructure of, 263Croniartium fusiforme aeciospores, morphology of,

245Cryptococcus neoformans, morphology of, 766Cryptococcus neoformans, nonencapsulated mutants

of, 1475Cryptococcus neoformans, phagocytosis by leukocytes,

1480cyst wall of Naegleria griiberi, 1272cytochrome c peroxidase activity in yeast, 1225cytochrome synthesis in Spirillum itersonii, 1648cytochromes of Pseudomonas syringae, 1785cytomembranes of Nitrosocystis oceanus, 422

dehydrogenases, electrophoretic variations in, 5443 - deoxy - D - arabino - heptulosonate - 7 - phosphate

synthetase, control patterns of, 15823-deoxy-D-arabinoheptulosonic acid-7-phosphate syn-

thetase, chromatography of, 1279deoxyribonucleic acid, antibiotics binding to, 576deoxyribonucleic acid, effect of 6-azathymine on, 2067deoxyribonucleic acid, segregation in bacteria, 415deoxyribonucleic acid, thymidine incorporation into,

1546deoxyribonucleic acid from streptomycetes, 1994deoxyribonucleic acid homology in Neisseria, 870deoxyribonucleic acid homology in Rhizobiaceae, 116deoxyribonucleic acid homology of bacteria, 486deoxyribonucleic acid of Bacillus stearothermophilus,

1805deoxyribonucleic acid of Haemophilus influenzae,

1802deoxyribonucleic acid repair replication after ir-

radiation, 1538deoxyribonucleic acid replication, mutant defective

in, 1603deoxyribonucleic acid synthesis, induction by nali-

dixic acid, 1664deoxyribonucleic acid synthesis in Flavobacterium

aurantiacum, 787deoxyribonucleic acid transfer in Escherichia coli,

396deoxyribosyl transfer by bacterial enzyme systems,

1551derepression of alkaline phosphatase by p-fluoro-

phenylalanine, 87desthiobiotin, as a precursor in Escherichia coli, 19302,6-diaminopurine, germinant for Bacillus macerans

spores, 1789

diethylaminoethyl dextran, inhibition of plaque-forming cells by, 791

diphtherial neuraminidase, purification of, 1565dipicolinic acid-less Bacillus cereus mutants, 2075division delay in Escherichia coli, 165drug resistance of enteric bacteria, 1166

ecology of fungi, 624electron transport in Staphylococcus aureus, 1868electrophoresis of dehydrogenases, use in bacterial

classification, 544electrophoresis of Mycoplasma proteins, 1807electrophoretic patterns of membrane proteins, 3593,6-endomethylene-1,2,3,6-tetrahydrophthalic acid,

use as buffer, 283endotoxin, influence of CC14, 1817endotoxin, production of Shwartzman reaction by, 590endotoxins from Salmonella mutants, 1320enteric bacteria, drug resistance of, 1166enterobacteria, characterization by electrophoresis

of dehydrogenases, 544enterobacteria, ribosomal ribonucleic acid cistrons

in, 1066Enterobacteriaceae, deoxyribonucleic acid homology

of, 486Enterobacteriaceae, osmotic shock in, 1934enterotoxin D, identification of, 1875enzyme control patterns in microorganisms, 1582enzyme synthesis in Pseudomonas putida mutant, 1970enzyme synthesis in streptomycin-dependent Es-

cherichia coli, 1154enzyme systems, ribosyl transfer by, 1551enzymes, hydrolytic, protection from heat, 1306enzymes, surface, release by osmotic shock, 1934erythritol, effect on Brucella melitensis, 1793erythrocyte concentration, effect on ,3-hemolysin, 1791erythrocytes, sensitization with Brucella antigens, 499Escherichia coli, action of myxin on 1837Escherichia coli, activity of colicinogenic factors in,

700Escherichia coli, alkaline phosphatase synthesis in, 87Escherichia coli, anaerobic growth of, 991Escherichia coli, aromatic pathway in, 1798Escherichia coli, biotin-requiring mutants, 2065Escherichia coli, chemotaxis in, 855Escherichia coli, colicin E susceptibility in, 677Escherichia coli, colicin factors in, 365Escherichia coli, colicin induction in, 691Escherichia coli, c olicin-tolerant mutations in, 1093

1112Escherichia coli, conjugation in, 396, 490, 1656Escherichia coli, deoxyribonucleic acid synthesis in,

1664Escherichia coli, desthiobiotin in, 1930Escherichia coli, distribution of R factors in, 1166Escherichia coli, effect of O-methylthreonine, 1890Escherichia coli, effect of mitomycin C on, 666Escherichia coli, B-galactosidase synthesis in, 2001Escherichia coli. -galactosidase transfer in, 1623Escherichia coli, glucose fermentation by, 488Escherichia coli, growth at low temperatures, 157Escherichia coli, growth on n-valeric acid, 1075Escherichia coli, inhibition by homoarginine, 712

2087VOL. 94, 1967

SUBJECr INDEX

Escherichia coli, inhibition by a-methyl-methionine,327

Escherichia coli, locus determining normal colonyformation, 1268

Escherichia coli, lysis measured by jl-galactosidaseactivity, 934

Escherichia coli, mannose-negative mutants, 1492Escherichia coli, mapping of mutation in, 778Escherichia coli, mutator gene of, 38Escherichia coli, ochre suppressors in, 388Escherichia coli, platinum ions in, 1915Escherichia coli, protein synthesis in damaged cells,

232Escherichia coli, R factor infection of, 2071Escherichia coli, radiation-induced division delay,

165Escherichia coli, recovery after irradiation, 1946Escherichia coli, regulation of glutamine synthetase

in, 949Escherichia coli, repair synthesis after irradiation,

1538Escherichia coli, D-serine deaminase system of, 1982,

1989Escherichia coli, streptomycin dependence in, 1275Escherichia coli, streptomycin-dependent, enzyme

synthesis in, 1154Escherichia coil, thymidine phosphorylase of, 1546Escherichia coli, thymine-requiring, selection, 1804Escherichia coli, urea production by, 1516Escherichia coli auxotroph, growth of, 1609Escherichia coli mutants, polyauxotrophic, 1684Escherichia coli mutants, polysaccharide production

in, 1492Escherichia coli mutants, recombination-deficient,

1266ethionine resistance in Saccharomyces cerevisiae,

1531ethylendiaminetetraacetate, effect on Escherichia

coli, 934euflavine, effect on Clostridium perfringens, 1437

F-like colicin factors in Escherichia coli, 365fatty acid composition of Corynebacterium acnes,

1300fatty acid requirements of cellulolytic rumen bacteria,

537fatty acids, effect of substrate on composition of,

1919fatty acids in Sarcina lutea, 333, 344, 349fecal microflora, effect of radiation on, 378, 383feedback inhibition of Escherichia coli glutamine

synthetase, 949Ferrobacillus ferrooxidans, oxidation of pyrite by

1046fibrinolytic activity in Pasteurella pestis, 19filters for preparation of absorption spectra, 1255fine structure of Bacillus megaterium, 1189fine structure of Bordetella bronchiseptica, 1216fine structure of Saccharomyces cerevisiae mito-

chondria, 1236fission of Schizosaccharomyces pombe, 192flagella, basal organelles of, 458flagellar antigen, antibodies combined with, 751flagellar coordination in Spirillum volutans, 1431

flagellation of Bacillus subtilis, 32flavensomycin, antifungal action of, 844Flavobacterium aurantiacwn, deoxyribonucle.c acid

synthesis in, 787fluorescent-antibody test for Actinomyces, 1287fluorescent pigment production by Pseudomonas,

1296p-fluorophenylalanine, derepression in Escherichia

coli, 87p-fluorophenylalinine, effect on chemotaxis in Es-

cherichia coli, 855freeze-etching technique in electron microscopy, 422fungi, antigenicity of, 914fungi, inhibition of lysis by melanins, 624fungi, phylogenetic study of, 1896fungi, tryptophan pathway in, 1896Fungi Imperfecti, physiological properties of, 597

galactosamine, N-acyl-, synthesis in cell walls, 1783#-galactosidase activity of Escherichia coli, 934g-galactosidase synthesis, repression of, 2001g-galactosidase transfer in bacterial conjugation, 1623gas chromatography-mass spectrometry, 333gene-enzyme relationships in Bacillus subtilis, 1706genetic analysis of Escherichia coli mutator strain, 38genetic control of flagellation, 32genetic control of tryptophan biosynthesis, 660genetic determinants, transfer in Nocardia, 650gentamicin complex, biological activity of, 789germination of putrefactive anaerobe 3679 spores, 506a-globulin, use by Treponema dentiwn, 1795glucan-degrading enzyme, role in Schizophyllum

commune mating, 1594(3(1-3)-glucanase, lytic action on yeast, 241glucose, effect on oxidation of ,-nitropropionic acid,

48glucose catabolism by meningopneumonitis agent

867glucose fermentation by Escherichia coli, 488glucose metabolism in Clostridiwn thermosaccharoly-

ticum, 530glucose metabolism of Streptococcus agalactiae, 184glutamate dehydrogenase, immunochemistry of, 958glutamate oxidation in mycobacteria, 92glutamic acid, N-acetyl-L-, in Neurospora crassa, 782glutamine synthetase, regulation in Escherichia

coli, 949glutamine synthetase from Bacillus licheniformis,

1007, 1016glycolipid from Salmonella minnesota mutant, 1824glyoxylate-condensing enzymes in Escherichia coli

1075gramicidin, effect on Streptococcus faecalis, 53gray lung agent in mouse lungs, 2048griseofulvin-producing Penicillium, 1787group Q streptococci, 291, 297

Haemophilus influenzae deoxyribonucleic acid, 1802Halobacterium halobium, ultrastructure of, 196hemagglutination tests with normal and tanned red

blood cells, 499a-hemolysin, action of, 1791,-hemolysin, phospholipase C activity of, 1313g-hemolysin, staphylococcal, purification, 300

2088 J. BAcnERioL.

SUBJECT INDEX

5-hemolysin, staphylococcal, 1327hemolysin of Bacillus cereus, 306heptoseless mutant of Salmonella minnesota, 1824hexose monophosphate pathway in Thiobacillus

ferrooxidans, 1052hexoses, inhibition of Salmonella typhimurium by, 707Histoplasma antigens, response of monkeys to, 1Histoplasma capsulatum, cell wall of, 466histoplasmosis, serological diagnosis of, 798homoaconitic acid in yeast mutant, 1060homoarginine, inhibition of Escherichia coli by, 712homoserine O-transsuccinylase, inhibition by

a-methylmethionine, 327host parasite interaction, role of phagocyte in, 1417,

1425hydrocarbons in Sarcina lutea, 333, 344, 349hydrogen peroxide, in phagocytes, 1417, 1425a-hydroxylglutaric acid, formation by Aspergillus

glaucus, 1560hydroxyurea, effect on protein synthesis, 1025hyphae, nuclear replication in, 1464

immune response to mycobacterial cell walls, 1736immune tolerance to Borrelia hispanica, 1359immune unresponsiveness, induction by capsular

antigen, 896immunoglobulins of antibodies reactive with Neisseria

gonorrhoeae antigens, 141indirect fluorescent-antibody procedure, 141induction of bacterial luciferase synthesis, 1638irradiation, deoxyribonucleic acid repair replication

after, 1538irradiation, effect on Candida albicans infections, 6irradiation, effect on Escherichia coli, 1946irradiation, effect on fecal microflora, 378, 383irradiation, effect on phagocytosis, 149isoniazid action on Mycobacterium tuberculosis, 793a-isopropylmalic acid, biosynthesis in Acetobacter

suboxydans, 512isovaleric acid, separation from 2-methyl-butyric

acid, 537

kanamycin resistance factor, replication of, 687Klebsiella pneumoniae capsular antigen, 896

L forms, streptococcal, capsular polysaccharideproduction by, 268

lac operon, transient repression of, 2001lactic streptococci, proteinase enzyme system of, 942Lactobacillus acidophilus, segregation of deoxy-

ribonucleic acid in, 415leaf nodules in Psychotria bacteriophila, 2027leptospires, growth at low temperatures, 27Leucothrix mucor, radiorespirometric studies of, 615leukemia, relationship of Mycoplasma to, 1797leukocytic hypersensitivity in streptococcal infections,

1722lipid synthesis in Saccharomyces cerevisiae, 61lipids of Staphylococcus aureus, 1854, 1868lipoic acid requirement of avian mycoplasma, 279lipopolysaccharides of Salmonella T mutants, 1366liver enzymes, influence of CC14, 1817luciferase, inducible synthesis of, 1638lysine-requiring yeast mutant, 1060

lysine transfer ribonucleic acid in Bacillus subtilisspores, 125

lysosyme requirement for spirochetolysis, 1388lytic action of ,B(1-3)-glucanase on yeast, 241lytic fission of Schizosaccharomyces pombe, 192

M protein, antibodies to, 274macromolecular synthesis in transformed cells, 131macromolecule synthesis in yeast spheroplasts, 1697macromolecules, synthesis by Escherichia coli, 157malate synthase, formation in Rhizopus nigricans,

1951maltose fermentation in Neisseria meningitidis, 557mannose-negative mutants of Escherichia coli, 1492marine bacterium, metabolism of, 1764melanin inhibition of lysis of fungi, 624membrane of yeast, effect of proteins on, 1590membrane proteins of Mycoplasma, 359membranes of Saccharomyces cerevisiae, 475meningopneumonitis agent, glucose catabolism by,

867meningopneumonitis organisms, cell walls of, 1178,

1184metabolite uptake in Neurospora crassa mutant, 1629methane analogues, inhibition of rumen methanogen-

esis by, 171methicillin-resistant Staphylococcus aureus, 1244methionine biosynthesis in yeast, 966methionine-cyano-B,2 auxotroph of Escherichia coli,

1609methionine-requiring Neisseria, genetics of, 719methionine synthesis in Escherichia coli, 3272-methylbutyric acid, separation from isovaleric acid,

537O-methylthreonine, effect on Escherichia coli, 1890Metschnikowia, ascospore numbers in, 258Micrococcus glutamicum, nutritional requirements,

323Micrococcus lysodeikticus mutants, radiation-sensi-

tive, 1270Micrococcus sodonensis, L-alanine production by,

1249microconidium formation in Neurospora crassa, 1757microcysts of Polysphondylium pallidum, 1143, 1150microsporidian, transmission in Swiss mice, 1340microtubule in Azotobacter vinelandii, 2062mitochondrial diversity in Saccharomyces cerevisiae,

1236mitomycin C, effect on Agrobacterium tumefaciens,

1470mitomycin C, effect on Bacillus megaterium, 1088mitomycin C, effect on colicin induction, 691mitomycin C, effect on Escherichia coli, 666monocytes, cellular immunity antibody in, 902, 907morphogenesis, in Myxococcus xanthus, 1883morphology of Cronartium fusiforme aeciospores, 245morphology of Cryptococcus neoformans, 766motility of Spirillum volutans, 1431muramic acid, N-acetyl-D-, role in cell wall biosyn-

thesis, 963mutant, Bacillus subtilis, phosphohydrolases of, 66mutant, Corynebacterium glutamicum, nutritional

requirements, 323mutant, Escherichia coli, colicin E-resistant, 677

2089VOL. 94, 1967

2090 SUBJEC1

mutant, Escherichia coli, colicin-tolerant, 1093, 1112mutant, Escherichia coli, polyauxotrophic, 1684mutant, Escherichia coli, recombination-deficient,

1266mutant, Micrococcus lysodeikticus, radiation-sensi-

tive, 1270mutant, Neurospora crassa, metabolite uptake in, 1629mutant, nonencapsulated, of Cryptococcus neoformans,

1475mutant, Pseudomonas putida, 1970mutant, Saccharomyces, 482mutant, Saccharomyces cerevisiae, 1136mutant, Saccharomyces cerevisiae, ethionine-resistant,

1531mutant, Salmonella, endotoxins from, 1320mutant, Salmonella, lipopolysaccharides of, 1366mutant, Salmonella typhimurium, effect of hexoses

on, 707mutant, of Schizosaccharomyces pombe, chemically

induced, 1162mutant, Staphylococcus aureus, lethal activity of, 13mutant, yeast, lysine-requiring, 1060mutant, yeast, ultraviolet-sensitive, 571mutants, biotin-requiring, 2065mutants, dipicolinic acid-less, 2075mutants, sporulation, 1957mutation in Escherichia coli, mapping of, 778mutations affecting aromatic pathway in Escherichia

coli, 1798mutator gene of Escherichia coli B, 38mycelial phase of Histoplasma capsulatum, cell walls

of, 466mycobacillin production by Bacillus subtilis, 2079mycobacteria, glutamate oxidation in, 92mycobacterial cell walls, oil-treated, effect on mice,

1736mycobacterial ribosomal fraction, immunogenic

activity of, 836Mycobacterium bovis, preservation at low tempera-

ture, 832Mycobacterium pellegrino, valine resistance in, 850Mycobacterium tuberculosis, antibody response to,

815Mycobacterium tuberculosis, isoniazid action on, 793Mycobacterium tuberculosis, surface culturing of, 1258mycoplasma, avian, lipoic acid requirement of, 279Mycoplasma, identification, 1807Mycoplasma, patterns of membrane proteins of, 359Mycoplasma, relationship to leukemia, 1797Mycoplasma gallisepticum, immune inactivation of,

403mycoplasma-like agents in mouse lung, 2048mycoplasma strains from cats, 1451mycoplasmas, adherence to surfaces, 1781myxin, action on Escherichia coli, 1837myxobacteria, fruiting, from South Carolina coast,

1253myxobacteria, ribosomal ribonucleic acid cistrons in,

1066Myxococcus xanthus, synthesis of ribonucleic acid

in, 1883

Naegleria griiberi, cyst wall of, 1272nalidixic acid, deoxyribonucleic acid synthesis in-

duced by, 1664

T INDEX J. BACTERIOL.

Neisseria, deoxyribonucleic acid homology in, 870Neisseria from clinical material, genetics of, 719Neisseria gonorrhoeae, antigens of, 141Neisseria meningitidis, labeling of nucleic acid of,

1262Neisseria meningitidis, mutation to sulfadiazine re-

sistance, 557nephelometric assay of antistaphylocoagulase serum,

821neuraminidase of Corynebacterium diptheriae, 1565Neurospora, glutamate dehydrogenase from, 958Neurospora, protection of trehalase in, 1306Neurospora crassa, cell wall of, 202Neurospora crassa, microconidium formation in,

1757Neurospora crassa, N-acetyl-L-glutamic acid in, 782Neurospora crassa, ribosomes from, 630, 1800Neurospora crassa mutant, metabolite uptake in, 1629nitrate-dark cells, luciferase synthesis in, 16386-nitropropionic acid, oxidation by Aspergillusflavus,

48Nitrosocystis oceanus, cytomembranes of, 422nitrous acid, effect on deoxyribonucleic acid, 1802Nocardia asteroides, ultrastructure of, 255Nocardia erythropolis, mating with Nocardia cani-

cruria, 650norethindrone, action on staphylococci, 1353Nosema, classification of microsporidian as, 1340nuclear replication in hyphae, 1464nucleic acid labeling of Neisseria meningitidis, 1262numerical classifications, reproducibility of, 826nutritional requirements of Bacillus coagulans, 517

ochre suppressors in Escherichia coli, 388oral cocci, classification, 1281oxygen concentration, effect on Candida utilis, 101oxygen sensitivity of anaerobic bacteria, 2012

Paracoccidioides brasiliensis, budding process of, 213Pasteurella pestis, distinction from P. pseudotubercu-

losis, 19penicillin, effect on Bacillus megaterium protoplasts,

884penicillin acyltransferase in Penicillium chrysogenum,

1502penicillinase induction with inorganic phosphate, 1672Penicillium, chitin in cell wall, 1787Penicillium chrysogenum, penicillin acyltransferase in,

1502Penicillium islandicum, synthesis of anthraquinoid pig-

ments in, 786Penicillium oxalicum, effect of flavensomycin on, 844peptidoglycan of Arthrobacter crystallopoietes cell

walls, 734, 741pertussis-sensitized mice, adrenergic blockade in, 804pesticins, relationship to fibrinolytic factor, 19phagocyte, role in host-parasite interaction, 1417, 1425phagocytosis, factors affecting, 149phagocytosis of Cryptococcus neoformans, 1480phenotypic resistance to valine, 850phosphate, inorganic, penicillinase induction with,

1672phosphohydrolases of Bacillus subtilis mutant, 66phospholipase C activity of ,3-hemolysin, 1313phospholipase of Bacillus cereus, 306

SUBJECT INDEX

phospholipases, effect on Escherichia coli, 934phospholipids of Thiobacillus thiooxidans, 1679phosphomannose isomerase-deficient mutants of Es-

cherichia coli, 1492, 1497photosynthetic bacteria, absorption spectra of, 1255photosynthetic growth of Rhodopseudomonaspalustris,

860Physarum polycephalum plasmodia, preservation of,

1257pipecolic acid, metabolism in Pseudomonas, 1034plaque-forming cells, inhibition by diethylaminoethyl

dextran, 791plasma factor in coccidioidomycosis, 919plasmodial myxomycetes, preservation of, 1257platelet damaging factor of staphylococcal a-toxin,

224platinum, effects on Escherichia coli, 1915pneumonia agent in mouse lungs, 2048polyacrylamide-gel electrophoresis, 1807polyamines, accumulation in Escherichia coli mutants,

1684polymorphonuclear neutrophils, metabolic changes

during phagocytosis, 149polysaccharide production in Escherichia coli mutants,

1492polysaccharide synthesis in Escherichia coli, suppres-

sion of, 388polysaccharides of cell wall peptidoglycans, 734Polysphondylium pallidum, microcysts of, 1143, 1150Propionibacteriwn, similarity to Corynebacterium,

1300propionic acid, catabolism by Aspergillus glaucus, 1560protein synthesis, effect of hydroxyurea on, 1205protein synthesis in Bacillus cereus spores, 177protein synthesis in cell-free extract of Staphylococcus

aureus, 80protein synthesis in damaged Escherichia coli, 232proteinase enzyme system of lactic streptococci, 942proteins, effect on yeast cell membrane, 1509proteins, mutant, immunochemistry of, 958Proteus vulgaris, kanamycin resistance in, 687protoplast formation from marine bacterium, 1764protoplasts of Bacillus megaterium, division of, 884pseudomonad, marine, metabolism of, 1764Pseudomonas, fluorescent pigment production by, 1296Pseudomonas, metabolism of pipecolic acid in, 1034Pseudomonas aeruginosa, aging of, 2077Pseudomonas aeruginosa, clear spot formation on

lawns of, 1251Pseudomonas putida, cold-sensitive mutant, 1970Pseudomonas syringae, cytochromes of, 1785Psychotria bacteriophila, leaf nodules in, 2027putrefactive anaerobe 3679 spores, 506putrescine biosynthesis by Escherichia coli, 1516pyocine, antibodies in combination with, 751pyridoxal neutralization of isoniazid action, 793pyrimidine-requiring Staphylococcus aureus mutant, 13pyrite oxidation, mechanism of, 1046pyruvate, effect on glucose metabolism, 530pyruvate metabolism in Sarcina maxima, 984

R factor infection of Escherichia coli, 2071R factors, distribution in Escherichia coli, 1166radiation-induced division delay in Escherichia coli,

165

radiation-sensitive mutants of Micrococcus lysodeik-ticus, 1270

radiation sensitivity, suppression in Escherichia coli,388

radiorespirometric studies of Leucothrix mucor, 615recombination-deficient mutants of Escherichia coli,

1266replication of kanamycin resistance factor, 687repression of ,B-galactosidase synthesis, 2001rhapidosomes, presence in bacteria, 1746Rhizobium, deoxyribonucleic acid of, 116Rhizopus nigricans, malate synthase formation in, 1951Rhodomicrobium vannielii, medium for, 1260Rhodopseudomonas palustris, effect of thiosulfate on

860Rhodopseudomonas palustris, thiosulfate-oxidizing en-

zyme in, 784Rhodospirillum, flagella of, 458ribonucleic acid, accumulation in Escherichia coli

mutants, 1684ribonucleic acid, synthesis in Myxococcus xanthus,

1883ribonucleic acid of Bacillus subtilis spores, 125ribosomal fraction of mycobacteria, immunogenic

activity of, 836ribosomal particles of Escherichia coli, 666ribosomal ribonucleic acid, regeneration, 1082ribosomal ribonucleic acid cistrons in bacteria, 1066ribosomes from Neurospora crassa, 630, 1800ribosyl transfer by bacterial enzyme systems, 1551Rickettsia prowazekii, ultrastructure of, 260Rickettsia rickettsii, infection of cotton rat with, 1334Rocky mountain spotted fever, ecology of, 1334rod-shaped structures in microorganisms, 1746rumen bacteria, volatile fatty acid requirement of, 537rumen methanogenesis, inhibition by methane ana-

logues, 171

Saccharomyces, tricarboxylic acid mutant of, 482Saccharomyces cerevisiae, ethionine-resistant mutant,

1531Saccharomyces cerevisiae, lipid synthesis in, 61Saccharomyces cerevisiae, membranes of, 475Saccharomyces cerevisiae, mitochondrial diversity,

1236Saccharomyces cerevisiae mutants, 1136Saccharomyces cerevisiae mutants, ultraviolet-sensi-

tive, 571Salmonella, somatic antigens in, 2018Salmonella minnesota, endotoxic glycolipid, 1824Salmonella mutants, endotoxins from, 1320Salmonella T mutants, lipopolysaccharides of, 1366Salmonella typhi, antibodies in combination with

flagella of, 751Salmonella typhimurium mutant, effect of hexoses on,

707salmonellosis, cellular immunity to, 902, 907sand, occurrence of fruiting myxobacteria in, 1253Sarcina lutea, fatty acids in, 333, 344, 349Sarcina lutea, hydrocarbons in, 333, 344, 349Sarcina maxima, pyruvate metabolism, 984Schizophyllum commune, mating of, 1594Schizosaccharomyces pombe, growth in unbalanced

medium, 192

VOL. 94, 1967 2091

SUBJECT INDEX

Schizosaccharomyces pombe mutants, chemically in-duced, 1162

D-serine deaminase system of Escherichia coli, 1982,1989

serological diagnosis of histoplasmosis, 798Shwartzman reaction, production by endotoxin, 590Sigmodon hispidus, infection with Rickettsia rickettsii,

1334skin test, effect on histoplasmosis diagnosis, 798somatic antigens in Salmonella, 2018spheroplast formation from marine bacterium, 1764spheroplasts, yeast, macromolecue synthesis in, 1697Spirillum itersonii, cytochrome synthesis in, 1648Spirillum volutans, flagellar coordination in, 1431spirochetolysis in guinea pig serum, 1388sporangiospores, release by Actinoplanes, 495spores of Bacillus cereus, protein synthesis in, 177spores of Bacillus macerans, germinant for, 1789spores of Bacillus megaterium, 434, 441spores of Bacillus popilliae, 759spores of Bacillus stearothermophilus, deoxyribonucleic

acid of, 1805spores of Bacillus subtilis, effect of L-alanine on, 522spores of Bacillus subtilis, ribonucleic acid of, 125spores of Bacillus thuringiensis, 486spores of Clostridium bifermentans, appendages of,

1206spores of putrefactive anaerobe 3679, germination of,

506sporulation in Bacillus cereus, chromosome incorpora-

tion during, 1715sporulation mutants, 1957staphylococcal coagulase, nephelometric assay of, 821staphylococcal a-hemolysin, activity of, 1791staphylococcal ,B-hemolysin, phospholipase C activity

of, 1313staphylococcal,-hemolysin, purification, 300staphylococcal 6-hemolysin, production, 1327staphylococcal a-toxin, physical states of, 1170staphylococcal a-toxin, platelet damaging factor of,

224staphylococci, action of norethindrone on, 1353staphylococci, enterotoxin in, 1875Staphylococcus aureus, alkaline phosphatase of, 780Staphylococcus aureus, methicillin-resistant, 1244Staphylococcus aureus, protein synthesis in cell-free

extract of, 80Staphylococcus aureus, repair after thermal injury,

1082Staphylococcus aureus electron transport system, 1868Staphylococcus aureus lipids, 1854, 1868Staphylococcus aureus mutants, lethal activity, 13Staphylococcus salivarius, reisolation, 1281streptococcal cell wall antigens, degradation, 1346streptococcal cell walls, association with arthritis, 1728streptococcal cell walls, biosynthesis of, 963streptococcal infections, leukocytic hypersensitivity in,

1722streptococcal L forms, capsular polysaccharide pro-

duction by, 268streptococcal M protein antibodies, 274streptococci, competence-provoking factor of, 75streptococci, group D, cell walls of, 1381streptococci, group Q, 291, 297

streptococci, lactic, proteinase enzyme system of, 942Streptococcus agalactiae, aerobic metabolism in, 184Streptococcus agalactiae, growth requirements, 1247Streptococcus avium, characterization, 291, 297Streptococcus bovis, arginine biosynthesis by, 1002Streptococcus faecalis, activation energy of, 1459Streptococcus faecalis, autolytic enzyme system of,

1525Streptococcus faecalis, cation permeability of, 53Streptococcus faecalis, deoxyribonucleic acid from,

2067Streptococcus faecium var. durans, transformation in,

1264Streptomyces steffisburgensis sp. n., 2022streptomycetes, carbohydrate metabolism in, 1520streptomycetes, deoxyribonucleic acid from, 1994streptomycin dependence in Escherichia coli, 1275streptomycin deprivation, effect on enzyme synthesis,

1154substructure of Bacillus subtilis cell wall, 1778sulfadiazine resistance in Neisseria meningitidis, 557sulfadiazine-resistant Neisseria, genetics of, 719surface culturing of Mycobacterium tuberculosis, 1258

taxonomy of psychrophilic Bacillus strains, 889temperature response of anthrax-infected animals, 552temperature-sensitive mutant of Bacillus subtilis, 1603tetanus toxin, isolation in monomeric form, 580tetanus toxoid formation, 586tetramethylpyrazine, accumulation in Corynebacte-

rium glutamicum, 323thermal injury, repair in Staphylococcus aureus, 1082thermosensitive replication of kanamycin resistance

factor, 687thiamine-dependent accumulation of tetramethyl-

pyrazine, 323thiazole, inhibition of synthesis by hexoses, 707thiobacilli, autotrophy in, 972Thiobacillus ferrooxidans, metabolic pathways in, 1052Thiobacillus neapolitanus, carbon dioxide fixation in,

409Thiobacillus novellus, catabolite repression in, 1484Thiobacillus thiooxidans, phospholipids of, 1679Thiobacillus thiooxidans cell walls, 2069thiosulfate, effect on Rhodopseudomonas palustris, 860thiosulfate-oxidizing enzyme in Rhodopseudomonas

palustris, 784threonine deaminase, inhibition by O-methylthre-

onine, 1890thymidine deprivation, effect on Bacillus megaterium,

1088thymidine phosphorylase, inhibition by uridine, 1546thymidine phosphorylase, reduced activity in Es-

cherichia coli, 778thymine-requiring Escherichia coli, selection, 1804thymineless Staphylococcus aureus, lethal activity of, 13Torula jeanselmei, physiological properties of, 597Torulopsis, in murine stomach, 1811a-toxin, platelet damaging factor of, 224a-toxin, staphylococcal, physical states of, 1170toxin, tetanus, isolation in monomeric form, 580toxin, type A botulinum, acetylcholinesterase inhibi-

tion by, 812toxin of Bacillus cereus, 306

2092 J. BACTERIOL.

VOL. 94, 1967 SUBJECT

toxoid formation, factors affecting, 586transformation in Streptococcus faecium var. durans,

1264trehalase, protection in Neurospora, 1306trehalose, synthesis in streptomycetes, 1520Treponema dentium, use of a-globulin, 1795tricarboxylic acid mutant of Saccharomyces, 482Trichophyton rubrum, antigenicity of, 914tryptophan biosynthesis in Bacillus subtilis, 660tryptophan pathway in fungi, 1896tuberculosis, transmission by low-temperature inocu-

lum, 832tumor induction by Agrobacteriwn tumefaciens, 1470

ultrastructure of Bacillus megaterium during sporo-genesis, 441

ultrastructure of Coxiella burnetil, 263ultrastructure of Halobacterium halobiwn, 196ultrastructure of Neurospora crassa, 1757ultrastructure of Nitrosocystis oceanus, 422ultrastructure of Nocardia asteriodes, 255ultrastructure of Rickettsia prowazekii, 260ultrastructure of small-colony variants of Staphylo-

coccus aureus, 1244ultraviolet light, yeast mutants sensitive to, 571uracil incorporation in sporulation mutants, 1957urea production by Escherichia coli, 1516

'INDEX 2093

uridine, effect on thymidine phosphorylase, 1546

vaccinated mice, resistance to Coccidioides immitis,924

vaccine, mycobacterial cell walls, 1736vaccine, robosomal-adjuvant, 836vaccine against coccidioidomycosis, 1394vaccine strain of Brucella melitensis, 1793n-valeric acid, growth of Escherichia coli on, 1075valine resistance in Mycobacterium pellegrino, 850valinomycin, effect on Streptococcus faecalis, 53Vi antigen, properties of, 1406, 1411virulence of aspergilli, 928vitamin requirements of streptococci, 297

yeast, cytochrome c peroxidase activity in, 1225yeast, localization in murine stomach, 1811yeast, lytic action of j3(1-3)-glucanase on, 241yeast, methionine biosynthesis in, 966yeast cell membrane, effect of proteins on, 1509yeast mutant, lysine-requiring, 1060yeast mutants, ultraviolet-sensitive, 571yeast spheroplasts, macromolecule synthesis in, 1697yeastlike phase of Histoplasma capsulatum, cell walls

of, 466

Zymomonas mobilis, activation energy of, 1459

INDEX TO DATE OF ISSUE

Month Date of Issue Pages

July 7/14/67 1-282August 8/24/67 283-494September 9/15/67 495-792October 10/30/67 793-1280November 11/22/67 1281-1806December 12/22/67 1807-2093

JOURNAL

OF

BACTERIOLOGY

VOLUME 94

BALTIMORE, MD. 212021967

EDITORIAL BOARDL. LEON CAMPBELL, Editor-in-Chief

University of lllinois, Urbana

L. S. BARON, Editor (1969)Walter Reed Army Institute of Research, Washington, D.C.

R. D. DeMOSS, Editor (1970)University ofIllinois, Urbana

L. JOE BERRY, Editor (1968)Bryn Mawr College, Bryn Mawr, Pa.

R. L. Anderson (1969)Robert Austrian (1969)W. Lane Barksdale (1969)S. S. Barkulis (1969)Alan W. Bernheimer (1969)Robert W. Bernlohr (1970)S. Gaylen Bradley (1969)Arnold F. Brodie (1970)Alan Campbell (1969)George B. Chapman (1969)V. P. Cirillo (1970)A. J. Clark (1969)V. W. Cochrane (1969)S. F. Conti (1969)C. D. Cox (1969)William J. Cromartie (1969)Roy Curtiss III (1969)A. L. Demain (1969)John A. DeMoss (1969)Michael Doudoroff (1969)Martin Dworkin (1970)Ellis Englesberg (1969)Frank B. Engley, Jr. (1969)Stanley Falkow (1969)William W. Ferguson (1969)Leonard Frank (1970)Martin Freundlich (1970)Herman Friedman (1969)

Lorraine Friedman (1968)Ruth E. Gordon (1969)Joseph S. Gots (1969)Leonard Hayffick (1969)James G. Hirsch (1969)John G. Holt (1969)Paul Howard-Flanders (1969)Jerard Hurwitz (1969)John Ingraham (1970)Arthur G. Johnson (1969)Elliot Juni (1967)R. E. Kallio (1969)Carl Lamanna (1970)Maurice Landy (1970)H. S. Levinson (1969)H. C. Lichstein (1970)W. R. Lockhart (1969)Donald G. Lundgren (1968)Elizabeth McCoy (1969)George B. Mackaness (1969)Stanley Marcus (1970)Julius Marmur (1968)A. G. Marr (1969)M. L. Morse (1969)Frederick C. Neidhardt (1969)Evelyn L. Oginsky (1969)Kenneth Paigen (1969)James T. Park (1969)Robert C. Parlett (1969)

Harry D. Peck (1970)Michael J. Pelczar, Jr. (1969)H. J. Phaff (1969)Roy Repaske (1969)H. V. Rickenberg (1970)C. F. Robinow (1969)W. R. Romig (1969)M. R. J. Salton (1969)Moselio Schaechter (1970)Russell W. Schaedler (1969)W. R. Sistrom (1969)Louis DS. Smith (1968)Paul F. Smith (1970)Roger Y. Stanier (1970)M. P. Starr (1970)B. A. D. Stocker (1969)Bernard S. Strauss (1969)Curtis B. Thorne (1969)H. E. Umbarger (1970)Lewis W. Wannamaker (1969)Arthur Weissbach (1969)R. W. Wheat (1969)Helen R. Whiteley (1969)J. B. Wilson (1969)Meyer J. Wolin (1970)Willis A. Wood (1969)Barbara E. Wright (1968)Frank E. Young (1970)

ROBERT A. DAY, Managing Editor, 4715 Cordell Ave., Bethesda, Md. 20014

EX OFFICIOS. E. LURIA, President (1967-1968)

Massachiusetts Institute of Technology, CambridgeDONALD E. SHAY, Secretary

University of Marylanid, Baltimore

DENNIS W. WATSON, Vice-President (1967-1968)Untiversity of Minnesota, Minneapolis

H. B. WOODRUFF, TreasurerMerck & Co., Inc., Rahway, N.J.

Published monthly by the American Society for Microbiology. Manufactured and distributed by Waverly Press, Inc., andThe Williams & Wilkins Co., at 428 E. Preston St., Baltimore, Md. 21202, U.S.A.

Subscription price $30.00 per year, single issue $4.00Second class postage paid at Baltimore, Md.

Made in the United States of America

CONTENTS

No. 1, JuLY 1967

Taxonomy, Ecology, Morphology and Structure, and Microbiological Methods

Isolation and Characterization of Actinomyces propionicus. Mary Ann Gerencser and JohnM. Slack .................................................................... 109

Deoxyribonucleic Acid Homology and Taxonomy of Agrobacterium, Rhizobium, and Chro-mobacterium. Gary T. Heberlein, J. DeLey, and R. Tijtgat......................... 116

Ultrastructure of the Obligate Halophilic Bacterium Halobacterium halobium. K. Y. Cho,Coline H. Doy, and E. H. Mercer.............................................. 196

Structure and Composition of the Cell Wall of Neurospora crassa. M. S. Manocha and J.Ross Colvin................................................................. 202

Cell Wall Changes during the Budding Process of Paracoccidioides brasiliensis and Blasto-myces dermatitidis. Luis M. Carbonell.......................................... 213

Fine Structure of Quiescent and Germinating Aeciospores of Cronartium fusiforme. CharlesH. Walkinshaw, James M. Hyde, and Janice Van Zandt........................... 245

Fine Structure of Nocardia asteroides Grown in a Chemically Defined Medium. DavoodFarshtchi and Norvel M. McClung............................................ 255

Ascospore Numbers in Metschnikowia. M. W. Miller, E. R. Barker, and J. I. Pitt......... 258Details of the Ultrastructure of Rickettsia prowazekii Grown in the Chick Yolk Sac. R. L.

Anacker, E. G. Pickens, and D. B. Lackman..................................... 260

Infection and Immunity

Serological Response of Rhesus Monkeys to Histoplasma, Blastomyces, and CoccidioidesAntigens. Robert L. Taylor....................................................I

Relationships of X Irradiation to the Enhancement of Candida albicans Infections. RobertS. Gordee and Patrick J. Simpson............................................. 6

Infection in Mouse Peritoneal Cavity with a Pyrimidine-requiring Mutant and NaturallyOccurring Staphylococcus aureus Strains. L. G. Mathieu, J. de Repentigny, S. Turgeon,and S. Sonea................................................................ 13

Pesticins. III. Expression of Coagulase and Mechanism of Fibrinolysis. E. D. Beesley, R. R.Brubaker, W. A. Janssen, and M. J. Surgalla.................................... 19

Differentiation of Pathogenic and Saprophytic Leptospires. I. Growth at Low Tempera-tures. Russell C. Johnson and Virginia G. Harris................................. 27

Natural and Immune Human Antibodies Reactive with Antigens of Virulent Neisseria gonor-rhoeae: Immunoglobulins G, M, and A. Irun R. Cohen........................... 141

Role of the Phagocyte in Host-Parasite Interactions. VIII. Effect of Whole-Body X Irradia-tion on Nicotinamides, Lysosomal Enzymes, and Bactericidal Activities of LeukocytesDuring Phagocytosis. R. J. Selvaraj and A. J. Sbarra............................. 149

Platelet Damaging Factor, a Fifth Activity of Staphylococcal a-Toxin. M. Manohar, S. K.Maheswaran, S. P. Frommes, and Robert K. Lindorfer........................... 224

Electron Microscopic Observations of Coxiella burnetii in the Guinea Pig. Joan Handley,D. Paretsky, and Johanna Stueckemann......................................... 263

Production of Capsular Polysaccharide (Hyaluronic Acid) by L Colonies of Group A Strep-tococci. Edward A. Mortimer, Jr., and Ellen L. Vastine........................... 268

Neuraminidase Activities of Clinical Isolates of Diplococcus pneumoniae. Richard T. Kelly,Silas Farmer, and Donald Greiff............................................... 272

Streptococcal M Protein Antibodies After Infection. R. W. Quinn and P. N. Lowry....... 274Amino Acid Composition of Crystalline Botulinum Toxin, Type A. David Stefanye, Edward

J. Schantz, and Leonard Spero................................................. 277

Genetics and Molecular Biology

Genetic Control of Flagellation in Bacillus subtilis. Terence M. Joys and Ruth W. Frankel. 32Mutator Gene of Escherichia coli B. Eli C. Siegel and Vernon Bryson.................... 38Cellular Sites for the Competence-provoking Factor of Streptococci. Roman Pakula...... 75

iii

COECONTENTSProtein Synthesis in a Cell-Free Extract from Staphylococcus aureus. James C.-H. Mao.... 80Derepression of Alkaline Phosphatase Synthesis in Escherichia coli by p-Fluorophenylalan-

ine. Soosang Kang and Alvin Markovitz........................................ 87

Medium-dependent Alteration of Lysine Transfer Ribonucleic Acid in Sporulating Bacillussubtilis Cells. Robert A. Lazzarini and Edith Santangelo........................... 125

Macromolecular Synthesis in Newly Transformed Cells of Bacillus subtilis. Charlotte Mc-Carthy and E. W. Nester...................................................... 131

Ordered Synthesis of Proteins During Outgrowth of Spores of Bacillus cereus T. AnnamarieTorriani and Cyrus Levinthal.................................................. 176

Protein Synthesis in Sonically Damaged Escherichia ccli. Harold Amos, Anneli Kuhn, andJanine Andre-Schwartz........................................................ 232

Microbial Physiology and MetabolismEffect of Glucose on the Oxidation of f-Nitropropionic Acid by Aspergillus flavus. George

E. Becker................................................................... 48

Gramicidin, Valinomycin, and Cation Permeability of Streptococcus faecalis. F. M. Haroldand J. R. Baarda............................................................. 53

Subcellular Sites Involved in Lipid Synthesis in Saccharomyces cerevisiae. Harold P. Klein,Carol M. Volkmann, and Morris A. Leaffer..................................... 61

Phosphohydrolases of a Bacillus subtilis Mutant Accumulating Inosine and Hypoxanthine.Arnold L. Demain and David Hendlin.......................................... 66

Inducible Glutamate Transport in Mycobacteria and Its Relation to Glutamate Oxidation.Richard H. Lyon, Palmer Rogers, Wendell H. Hall, and Herman C. Lichstein........ 92

Aerobic Microbial Growth at Low Oxygen Concentrations. Marvin J. Johnson ......... 101

Synthesis of Macromolecules by Escherichia coli near the Minimal Temperature for Growth.Maxwell K. Shaw and John L. Ingraham........................................ 157

Division Delay Induced in Escherichia coli by Near-Ultraviolet Radiation. Stephen L. Phil-lips, Stanley Person, and John Jagger............................................ 165

Inhibition of Ruman Methanogenesis by Methane Analogues. T. Bauchop............... 171Aerobic Metabolism of Streptococcus agalactiae. M. N. Mickelson..................... 184

Growth of the Fission Yeast, Schizosaccharomyces pombe, with Late, Eccentric, Lytic Fis-sion in an Unbalanced Medium. Byron F. Johnson............................... 192

Lytic Action of,(1-3)-Glucanase on Yeast Cells. J. Monreal, F. de Uruburu, and J. R.Villanueva................................................................... 241

Lipoic Acid Requirement of Mycoplasma Species Avian Strain J. Pauline G. Lund andMary S. Shorb............................................................... 279

Errata...................................................................... 281

No. 2, AUGUST 1967

Taxonomy, Ecology, Morphology and Structure, andMicrobiological Methods

A pH 7 Buffer Devoid of Nitrogen, Sulfur, and Phosphorus for Use in Bacteriological Sys-tems. M. F. Mallette.......................................................... 283

Group Q Streptococci. I. Ecology, Serology, Physiology, and Relationship to EstablishedEnterococci. Sandra Simpson Nowlan and R. H. Deibel........................... 291

Group Q Streptococci.II. Nutritional Characteristics and Growth Relationship to Thymine,Folate, and Folinate. Sandra Simpson Nowlan and R. H. Deibel................... 297

Electrophoretic Patterns of Membrane Proteins of Mycoplasma. Shlomo Rottem andShmuel Razin ................................................................ 359

Fine Structure of the Cytomembranes of Nitrosocystis oceanus. Charles C. Remsen, Freder-ica W. Valois, and Stanley W. Watson.......................................... 422

Fine Structure of Bacillus megaterium during Microcycle Sporogenesis. John H. Freer andHillel S. Levinson............................................................ 441

Basal Organelles of Bacterial Flagella. Germaine Cohen-Bazire and Jack London.......... 458

Comparative Study of the Cell Walls of the Yeastlike and Mycelial Phases of Histoplasmacapsulatum. Judith E. Domer, James G. Hamilton, and James C. Harkin............ 466

Membranes of Saccharomyces cerevisiae. Harold P. Klein, Carol Volkmann, and JosephWeibel .........

475

IV

CONTENTS

Electron Microscopy of a Tricarboxylic Acid Mutant of Saccharomyces. W. D. Bowers, Jr.,D. 0. McClary, and M. Ogur.................................................. 482

Biphasic System for Separation of Spores and Crystals of Bacillus thuringiensis. N. S. Good-man, R. J. Gottfried, and M. H. Rogoff......................................... 485

Deoxyribonucleic Acid Homologies Among Some Bacteria. Don J. Brenner, Malcolm A.Martin, and Bill H. Hoyer..................................................... 486

Infection and Immunity

Staphylococcal ,-Hemolysin. I. Purification of ,B-Hemolysin. S. K. Maheswaran, K. L.Smith, and Robert K. Lindorfer................................................ 300

Lethal Toxin of Bacillus cereus. I. Relationships and Nature of Toxin, Hemolysin, and Phos-pholipase. Charles E. Johnson and Peter F. Bonventre............................ 306

Studies of Intestinal Microflora. VI. Effect of X Irradiation on the Fecal Microflora of theRat. Albert S. Klainer, Sherwood Gorbach, and Louis Weinstein................... 378

Studies of Intestinal Microflora. VII. Effect of Diet and Fecal Microbial Flora on Survivalof Animals Exposed to X Irradiation. Albert S. Klainer, Sherwood Gorbach, and LouisWeinstein................................................................... 383

Role of Complement in Immune Inactivation of Mycoplasma gallisepticum. L. F. Barkerand J. K. Patt............................................................... 403

Genetics and Molecular Biology

A Comparative Study of Two F-like Colicin Factors, ColV2 and ColV3, in Escherichia coliK-12. Adelaide C. MacFarren and R. C. Clowes................................. 365

Suppression of Radiation Sensitivity and Capsular Polysaccharide Synthesis in EscherichiacoliK-12 by Ochre Suppressors. Alvin Markovitz and Bruce Baker................. 388

Role for the Female in Bacterial Conjugation in Escherichia coli. David Freifelder........ 396Segregation of Deoxyribonucleic Acid in Bacteria: Association of the Segregating Unit with

the Cell Surface. Ning-Chun Chai and Karl G. Lark.............................. 415Energy Requirements for Specific Pair Formation During Conjugation in Escherichia coli

K-12. Roy Curtiss III and D. R. Stallions ........... . ............................. 490Catabolite Repression Is Not Due to End-Product Repression in the gal Operon. Kenneth

Paigen, Beverly Williams, and James McGinnis.................................. 493

Microbial Physiology and Metabolism

Formation of Adenosine Cyclic 3', 5'-Phosphate by Nonproliferating Cells and Cell-freeExtract of Brevibacterium liquefaciens. Misao Ide, Akihiro Yoshimoto, and TadashiOkabayashi.................................................................. 317

Thiamine-dependent Accumulation of Tetramethylpyrazine Accompanying a Mutation inthe Isoleucine-Valine Pathway. A. L. Demain, M. Jackson, and N. R. Trenner .... .... 323

Inhibition of Growth of Escherichia coli and of Homoserine O-Transsuccinylase by a-Methylmethionine. Sondra Schlesinger ........ .................................. 327

Identification of Fatty Acids and Aliphatic Hydrocarbons in Sarcina lutea by Gas Chroma-tography and Combined Gas Chromatography-Mass Spectrometry. T. G. Tornabene,E. Gelpi, and J. Oro. ............... ........................................ 333

Fatty Acid and Aliphatic Hydrocarbon Composition of Sarcina lutea Grown in Three Dif-ferent Media. T. G. Tornabene, E. 0. Bennett, and J. Oro.......................... 344

14C Incorporation into the Fatty Acids and Aliphatic Hydrocarbons of Sarcina lutea. T. G.Tornabene and J. Oro......................................................... 349

Effect of Adenosine Monophosphate, Adenosine Diphosphate, and Reduced NicotinamideAdenine Dinucleotide on Adenosine Triphosphate-dependent Carbon Dioxide Fixa-tion in the Autotroph Thiobacillus neapolitanus. Jerry V. Mayeux and Emmett J. John-son...................................................................... 409

Metabolic Requirements for Microcycle Sporogenesis of Bacillus megaterium. P. K. Holmesand Hillel S. Levinson........................................................ 434

Aldehydes and Ketones Produced During Fermentation of Glucose by Escherichia coli.Donald W. Thayer and James E. Ogg........................................... 488

v

CONTENTS

No. 3, SEPTEMBER 1967

Taxonomy, Ecology, Morphology and Structure, and Microbiological Methods

Release of Sporangiospores by a Strain of Actinoplanes. Michael L. Higgins............. 495Characterization of Enterobacteria by Starch-Gel Electrophoresis of Glucose-6-Phosphate

Dehydrogenase and Phosphogluconate Dehydrogenase. James E. Bowman, RobertR. Brubaker, Henri Frischer, and Paul E. Carson................................ 544

Sphere-Rod Morphogenesis in Arthrobacter crystallopoietes. I. Cell Wall Composition andPolysaccharides of the Peptidoglycan. Terry A. Krulwich, Jerald C. Ensign, Donald J.

Tipper, and Jack L. Strominger................................................ 734Sphere-Rod Morphogenesis in Arthrobacter crystallopoietes. II. Peptides of the Cell Wall

Peptidoglycan. Terry A. Krulwich, Jerald C. Ensign, Donald J. Tipper, and Jack L.

Strominger.................................................................. 741Comparisons of Cells, Refractile Bodies, and Spores of Bacillus popilliae. Brij M. Mitruka,

Ralph N. Costilow, S. H. Black, and R. E. Pepper............................... 759Micromorphology of Cryptococcus neoformans. Mercedes R. Edwards, Morris A. Gordon,

Edward W. Lapa, and William C. Ghiorse...................................... 766

Infection andImmunity

Differences Between Brucella Antigens Involved in Indirect Hemagglutination Tests withNormal and Tanned Red Blood Cells. Ramon Diaz, Lois M. Jones, Daniel Leong, andJ. B. Wilson................................................................. 499

Temperature Response in AnimalsInfected with Bacillus anthracis. Jerry S. Walker, Freder-ick Klein, Ralph E. Lincoln, and Albert L. Fernelius............................. 552

Relationship Between Sulfadiazine Resistance and the Failure to Ferment Maltose in Neis-seria meningitidis. David T. Kingsbury.......................................... 557

Tetanus Toxin and Antigenic Derivatives. I. Purification of the Biologically Active Mono-mer. Samuel G. Murphy and Kent D. Miller.................................... 580

Tetanus Toxin and Antigenic Derivatives.II. Effect of Protein and Formaldehyde Concen-tration on Toxoid Formation. Samuel G. Murphy................................ 586

Failure of Bacterial Endotoxin to Produce the Generalized Shwartzman Reaction in theSyrian Hamster. Katherine Merritt and Michael Galton........................... 590

Preliminary Studies of Some Physiological Properties of Torula jeaniselmei. C. J. K. Wangand Sandra Brownell......................................................... 597

In Vivo Growth and Distribution of Anthrax Bacilli in Resistant, Susceptible, and Immu-nized Hosts. WilliamI. Jones, Jr., Frederick Klein, Jerry S. Walker, Bill G. Mahlandt,James P. Dobbs, and Ralph E. Lincoln......................................... 600

Antibiotic Treatment of Anthrax Infection in Mice. WilliamI. Jones, Jr., Frederick Klein,Ralph E. Lincoln, Jerry S. Walker, Bill G. Mahlandt, and James P. Dobbs ......... . 609

Electron Microscopy of the Combination of Antibodies with Flagellar Antigen and with a

Pyocine. Sachio Goto, J. Yusuru Homma, and Stuart Mudd....................... 751Inhibition of Deoxyribonucleic Acid Synthesis in Flavobacterium aurandtiacum by Aflatoxin

B1. E. B. Lillehoj and A. Ciegler............................................... 787Biological Activity of the Antibiotic Components of the Gentamicin Complex. Marvin J.

Weinstein, Gerald H. Wagman, Edwin M. Oden, and Joseph A. Marquez........... 789Inhibition of Plaque-forming Cells by Diethylaminoethyl Dextran. P. A. Campbell, J. Cald-

well, and P. Kind ............................................................ 791

Genetics and Molecular Biology

Development of Competence in the Bacillus subtilis Transformation System. Kenneth F.Bott and Gary A. Wilson..................................................... 562

Mutants of Yeast Sensitive to Ultraviolet Light. Richard Snow......................... 571Comparative Studies with Three Antibiotics Binding to Deoxyribonucleic Acid. Fritz Reus-

ser and Bijoy K. Bhuyan...................................................... 576Ribosomes and Ribosomal Protein from Neurospora crassa. I. Physical, Chemical, and

Immunochemical Properties. F. A. M. Alberghina and S. R. Suskind............... 630

Vi

CONTENTS

Linkage and Segregation of Unselected Markers in Matings of Nocardia erythropolis withNocardia canicruria. George H. Brownell and James N. Adams..................... 650

Transformation Mapping of the Genes Controlling Tryptophan Biosynthesis in Bacillussubtilis. Bruce C. Carlton...................................................... 660

Decomposition of Ribosomal Particles in Escherichia coli Treated with Mitomycin C.Hiromi Suzuki and Wendell W. Kilgore......................................... 666

Genetic Basis of Colicin E Susceptibility in Escherichia coli. I. Isolation and Properties ofRefractory Mutants and the Preliminary Mapping of Their Mutations. Caroline Hilland I. B. Holland............................................................ 677

Thermosensitive Replication of a Kanamycin Resistance Factor. Yoshiro Terawaki, HisaoTakayasu, and Tomoichiro Akiba ........... ................................... 687

Comparative Study of the Events Associated with Colicin Induction. Harvey R. Herschmanand Donald R. Helinski....................................................... 691

Effect of Rec- Mutations on the Activity of Colicinogenic Factors. Donald R. Helinskiand Harvey R. Herschman.................................................... 700

Growth Inhibition by Hexoses of a Temperature-sensitive, Thiazoleless Mutant of Sal-monella typhimurium. Jose L. Parada and Manuel V. Ortega....................... 707

Inhibition of Escherichia coli B by Homoarginine. Graciela M. Peyru and Wemer K. Maas. . 712Sulfadiazine-resistant and Methionine-requiring Neisseria Isolated from Clinical Material.

B. Wesley Catlin ............................................................. 719Genetic Mapping of a Mutation in Escherichia coli Showing Reduced Activity of Thymi-

dine Phosphorylase. Beverly Dale and G. Robert Greenberg....................... 778

Microbial Physiology and MetabolismSequence of Events during Germination of Putrefactive Anaerobe 3679 Spores. Minoru

Uehara and Hilmer A. Frank ................................................. 506Biosynthesis of a-Isopropylmalic and Citric Acids in Acetobacter suboxydans. Michael E.

Maragoudakis and Murray Strassman.......................................... 512Growth of Bacillus coagulans in Chemically Defined Media. Rosemarie Marshall and R. J.

Beers................................................................... 517Separation of Two Functional Roles of L-Alanine in the Initiation of Bacillus subtilis Spore

Germination. Richard Wax, Ernst Freese, and Michael Cashel..................... 522Regulatory Effect of Pyruvate on the Glucose Metabolism of Clostridium thermosaccharoly-

ticum. Chin K. Lee and Z. John Ordal.......................................... 530Volatile Fatty Acid Requirements of Cellulolytic Rumen Bacteria. B. A. Dehority, H. W.

Scott, and Patricia Kowaluk................................................... 537Radiorespirometric Studies of Leucothrix mucor. H. D. Raj ...... ...................... 615Inhibition of the Lysis of Fungi by Melanins. M.-J. Kuo and M. Alexander............. 624Repressible Alkaline Phosphatase of Staphylococcus aureus. D. B. Shah and H. Blobel.... 780Deacetylation of N-Acetyl-L-Glutamic Acid by Neurospora crassa. D. S. Hoare, S. Louise

Hoare, and Joe Brame........................................................ 782Induction of a Thiosulfate-oxidizing Enzyme in Rhodopseudomonas palustris. James P. Rolls

and E. S. Lindstrom.......................................................... 784Biotin and the Biosynthesis of Anthraquinoid Pigments in Penicillium islandicum Sopp.

Yoshio Ueno ................................................................ 786

No. 4, OCTOBER 1967

Taxonomy, Ecology, Morphology and Structure, and Microbiological MethodsFactors Affecting Reproducibility of Numerical Classification. W. R. Lockhart.......... 826Comparative Cell Wall Analyses of Morphological Forms within the Genus Actinomyces.

Leo Pine and Clarence J. Boone................................................ 875Growth and Division of Protoplasts of Bacillus megaterium and Inhibition of Division by

Penicillin. Iwao Kusaka....................................................... 884Taxonomy of Psychrophilic Strains of Bacillus. J. M. Larkin and J. L. Stokes.............. 889Unrelatedness of Bacillus amyloliquefaciens and Bacillus subtilis. N. E. Welker and L. Leon

Campbell ................................................................... 1124

.ii

CONTENTS

Microcysts of the Cellular Slime Mold Polysphondylium pallidum. I. Factors InfluencingMicrocyst Formation. Mohamed A. Toama and Kenneth B. Raper................. 1143

Microcysts of the Cellular Slime Mold Polysphondylium pallidum. II. Chemistry of the Micro-cyst Walls. Mohamed A. Toama and Kenneth B. Raper........................... 1150

Preparation and Chemical Composition of the Cell Walls of Mature Infectious Dense Formsof Meningopneumonitis Organisms. G. P. Manire and Akira Tamura............... 1178

Preparation and Chemical Composition of the Cell Membranes of Developmental Reticu-late Forms of Meningopneumonitis Organisms. Akira Tamura and G. P. Manire...... 1184

Fine Structure of Bacillus megaterium During Synchronous Growth. D. J. Ellar, D. G. Lund-gren, and R. A. Slepecky...................................................... 1189

Appendages of Clostridium bifermentans Spores. Leodocia Pope, Diane P. Yolton, and L. J.Rode...................................................... 1206

Electron Microscopy of a Strain of Bordetella bronchiseptica. G. W. Richter and YvonneKress. 1216

Distribution of Cytochrome c Peroxidase Activity in Wild-Type and Petite Cells of Bakers'Yeast Grown Aerobically and Anaerobically. Charlotte J. Avers.................... 1225

Ultrastructure of Small Colony Variants of a Methicillin-resistant Staphylococcus aureus.Roger J. Bulger and Ruth Ellen Bulger.......................................... 1244

Fruiting Myxobacteria from the South Carolina Coast. Ellis R. Brockman............... 1253Use of Glass Fiber Filters for the Rapid Preparation of in Vivo Absorption Spectra of Photo-

synthetic Bacteria. Hans G. Truper and Charles S. Yentsch...................... 1255Preservation of Physarum polycephalum Plasmodia In Liquid Nitrogen. George B. Boder

and Robert W. Johnson....................................................... 1257Improved Surface Culturing of Mycobacterium tuberculosis. Mayer B. Goren............. 1258Defined Medium Allowing Maximal Growth of Rhodomicrobium vannielii. William C. Tren-

tini...................................................................... 1260Isolation and Preliminary Chemical Analysis of the Cyst Wall of the Amoeba-Flagellate

Naegleria griiberi. J. M. Werth and A. J. Kahn.................................. 1272

Infection and Immunity

Mechanism for the Pyridoxal Neutralization of Isoniazid Action on Mycobacterium tuber-culosis. William H. Beggs and John W. Jenne.................................... 793

Effects of a Single Histoplasmin Skin Test on the Serological Diagnosis of Histoplasmosis.Leo Kaufman, Robert T. Terry, Joseph H. Schubert, and David McLaughlin........ 798

In Vivo and in Vitro Manifestations of Adrenergic Blockade in Bordetella pertussis-vacci-nated Mice. Kenneth F. Keller and Charles W. Fishel............................. 804

In Vitro Acetylcholinesterase Inhibition by Type A Botulinum Toxin. R. Marshall and L. Y.Quinn .............. .. .. 812

Kinetics and Immunoglobulin Nature of the Antibody Response to Mycobacterium tubercu-losis. J. E. O'Connor and I. S. Snyder........................................... 815

Nephelometric Assay of Bovine Antistaphylocoagulase Serum. Fred J. Stutzenberger andC. L. San Clemente........................................................... 821

Host-Parasite Relationships in Experimental Airborne Tuberculosis. II. Reproducible Infec-tion by Means of an Inoculum Preserved at -70 C. A. A. Grover, H. K. Kim, E. H.Wiegeshaus, and D. W. Smith................................................. 832

Preparation and Effect of Different Adjuvants on the Immunogenic Activity of Mycobac-terial Ribosomal Fraction. Anne S. Youmans and Guy P. Youmans................ 836

Influence of Cellular Factors on Immune Unresponsiveness Induced by Klebsiella pneu-moniae Capsular Antigen. William G. Wu and Karen L. Trice..................... 896

Experimental Salmonellosis. X. Cellular Immunity and Its Antibody in Mouse MononuclearPhagocytes. Satonori Kurashige, Nobutaka Osawa, Masaya Kawakami, and SusumuMitsuhashi.................................................................. 902

Experimental Salmonellosis. XI. Induction of Cellular Immunity and Formation of Anti-body by Transfer Agent of Mouse Mononuclear Phagocytes. Susumu Mitsuhashi,Kazuko Saito, Nobutaka Osawa, and Satonori Kurashige ......................... 907

Factors Affecting the Antigenicity of Trichophyton rubrum. Andrew J. Stuka and RobertBurrell ...................................................................... 914

.i.

CONTENTS

Cytolytic Plasma Factor in Experimental Coccidioidomycosis. Joseph W. Landau and Vic-tor D. Newcomer................................... ........................ 919

Resistance of Vaccinated Mice to Typical and Atypical Strains of Coccidioides immitis.Milton Huppert, H. B. Levine, S. H. Sun, and E. J. Peterson...................... 924

Experimental Study of the Pathogenicity of Aspergilli for Mice. Scott Ford and LorraineFriedman................................................................... 928

Immunochemical Studies on Glutamate Dehydrogenase and on Two Mutant Forms of theProtein. David B. Roberts..................................................... 958

Physical States of Staphylococcal cz-Toxin. John P. Arbuthnott, John H. Freer, and AlanW. Bernheimer............................................................... 1170

Enhancement of Clear Spot Formation on Pseudomonas aeruginosa Lawns by Chloram-phenicol, Tetracycline, and Sulfisoxazole. Joseph Fedorko, Sol Katz, and Hedi All-noch ...................................................................... 1251

Genetics and Molecular Biology

Deoxyribonucleic Acid Homologies among Species of the Genus Neisseria. David T. Kings-bury...................................................................... 870

Studies with Hydroxyurea. VII. Hydroxyurea and the Synthesis of Functional Proteins.Herbert S. Rosenkranz, Elaine B. Winshell, Aiga Mednis, Howard S. Carr, and Cor-nelia J. Ellner................................................................ 1025

Comparative Study of Ribosomal Ribonucleic Acid Cistrons in Enterobacteria and Myxo-bacteria. Richard L. Moore and Brian J. McCarthy............................... 1066

Function of the Glyoxylate-condensing Enzymes. I. Growth of Escherichia coli on n-ValericAcid. Philip Furmanski, Warner S. Wegener, Henry C. Reeves, and Samuel J. Aj....... 1075

Regeneration of Ribosomes and Ribosomal Ribonucleic Acid During Repair of ThermalInjury to Staphylococcus aureus. Stephen J. Sogin and Z. John Ordal............... 1082

Effects of Mitomycin C and Thymidine Deprivation on Lysogenic and Sensitive Strains ofBacillus megaterium. W. F. Maisch and J. T. Wachsman.......................... 1088

Interaction of Colicins with Bacterial Cells. III. Colicin-tolerant Mutations in Escherichiacoli. M. Nomura and C. Witten................................................ 1093

Genetics and Physiology of Colicin-tolerant Mutants of Escherichia coli. Rosa Nagel deZwaig and S. E. Luria ................. ....................................... 1112

Some Novel Mutants of Saccharomyces cerevisiae Inhibited by Adenosylmethionine andAdenosylhomocysteine. Kemet D. Spence and Stanley K. Shapiro.................. 1136

Effect of Streptomycin Deprivation on Enzyme Synthesis in Streptomycin-dependent Es-cherichia coli. R. E. Goodman and Charles R. Spotts............................. 1154

Intragenic Mapping of Chemically Induced ad-7 Mutants of Schizosaccharomyces pombe.Nicola Loprieno............................................................. 1162

Drug Resistance of Enteric Bacteria. XIII. Distribution of R Factors in Escherichia coliStrains Isolated from Livestock. Susumu Mitsuhashi, Hajime Hashimoto, and KanameSuzuki..................................................................... 1166

Fine-Structure Analysis of Intercellular and Intracellular Mitochondrial Diversity in Sac-charomyces cerevisiae. Micheline Federman and Charlotte J. Avers................. 1236

Use of Exogenous Adenine to Label the Nucleic Acids of Wild-Type Neisseria meningitidis.David T. Kingsbury and John F. Duncan....................................... 1262

Evidence of Genetic Transformation in Streptococcus faecium var. durans (SF/0). Bipin M.Mehta, Francis M. Sirotnak, and Dorris J. Hutchison............................. 1264

Formation of Deletion Mutations in Recombination-deficient Mutants of Escherichia coli.Joseph Inselburg.............................................................. 1266

Locus Determining Normal Colony Formation on the Chromosome of Escherichia coli K-12.A. Sas'arman and Thea Horodniceanu........................................... 1268

Ultraviolet Radiation-sensitive Mutants of Micrococcus lysodeikticus. Rose R. Feiner...... 1270Reversion from Streptomycin Dependence in Escherichia coli by a Further Change in the

Ribosome. David Apirion and David Schlessinger................................ 12755-Bromouracil Utilization by Bacillus subtilis. Charles D. Laird and Walter F. Bodmer.... 1277Chromatography of 3-Deoxy-D-Arabinoheptulosonic Acid-7-Phosphate Synthetase (trp)

on Diethylaminoethyl Cellulose: a Correction. B. J. Wallace and J. Pittard.......... 1279

iX

CONTENTS

Microbial Physiology and Metabolism

Flavensomycin, an Inhibitor of Enzyme Reactions Involving Hydrogen Transfer. DavidGottlieb and Yukio Inoue..................................................... 844

Induced Phenotypic Resistance to Valine in Mycobacterium pellegrino. Istvan Horvath, A.Szentirmai, and J. Zsadanyi................................................... 850

Effects of p-Fluorophenylalanine and Chloramphenicol on Chemotaxis in Escherichia coli.R. W. Fleming, F. D. Williams, and K. A. Wailes................................ 855

Effect of Thiosulfate on the Photosynthetic Growth of Rhodopseudomonas palustris. James P.Rolls and E. S. Lindstrom..................................................... 860

Initial Step in Catabolism of Glucose by the Meningopneumonitis Agent. Joyce Vender andJames W. Moulder........................................................... 867

Lysis of Escherichia coli by Ethylenediaminetetraacetate and Phospholipases as Measuredby ,3-Galactosidase Activity. Milton W. Slein and Gerald F. Logan, Jr............... 934

Proteinase Enzyme System of Lactic Streptococci. II. Role of Membrane Proteinase inCellular Function. R. A. Cowman, H. E. Swaisgood, and M. L. Speck.............. 942

Regulation of Glutamine Synthetase. X. Effect of Growth Conditions on the Susceptibilityof Escherichia coli Glutamine Synthetase to Feedback Inhibition. Henry S. Kingdonand E. R. Stadtman.......................................................... 949

Biosynthesis of Streptococcal Cell Walls: N-Acetyl-D-Muramic Acid. S. S. Barkulis, J. J.Boltralik, H. Hankin, and H. Heymann......................................... 963

Role of S-Adenosylmethionine in Methionine Biosynthesis in Yeast. J. L. Botsford andL. W. Parks................................................................. 966

Biochemical Basis of Obligate Autotrophy in Blue-Green Algae and Thiobacilli. Arnold J.Smith, Jack London, and Roger Y. Stanier...................................... 972

Pyruvate Metabolism in Sarcina maxima. Dorothy G. Kupfer and E. Canale-Parola ....... 984Anaerobic Growth Yields of Aerobacter cloacae and Escherichia coli. Eovaldo Hernandez

and Marvin J. Johnson ...................................................... 991Energy Supply and Cell Yield in Aerobically Grown Microorganisms. Eovaldo Hernandez

and Marvin J. Johnson....................................................... 996Arginine Biosynthesis by Streptococcus bovis. R. A. Niederman and M. J. Wolin......... 1002Regulation of Glutamine Synthetase. V. Partial Purification and Properties of Glutamine

Synthetase from Bacillus licheniformis. Jerry S. Hubbard and E. R. Stadtman........ 1007Regulation of Glutamine Synthetase. VI. Interactions of Inhibitors for Bacillus licheniformis

Glutamine Synthetase. Jerry S. Hubbard and E. R. Stadtman...................... 1016Metabolism of Pipecolic Acid in a Pseudomonas Species. V. Pipecolate Oxidase and Dehy-

drogenase. Marietta L. Baginsky and Victor W. Rodwell.......................... 1034Mode of Action of Antibiotic U-24,544. Fritz Reusser................................ 1040Mechanism of Bacterial Pyrite Oxidation. Melvin P. Silverman........................ 1046Evidence for the Calvin Cycle and Hexose Monophosphate Pathway in Thiobacillus ferro-

oxidans. Nord L. Gale and Jay V. Beck................................... .... 1052Homoaconitic Acid Accumulation by a Lysine-requiring Yeast Mutant. Michael E. Mara-

goudakis........................................................... 1060Comparison of the a-Amylase of Bacillus subtilis and Bacillus amyloliquefaciens. N. E. Wel-

ker and L. Leon Campbell ................................................... 1131Requirements for Growth of Streptococcus agalactiae in a Chemically Defined Medium.

Norman P. Willett, Guy E. Morse, and Sharon A. Carlisle........................ 1247Production of L-Alanine by Biotin-deficient Micrococcus sodonensis. Jerome J. Perry...... 1249

No. 3, NOVEMBER 1967

Taxonomy, Ecology, Morphology and Structure, and Microbiological Methods

Reisolation of Staphylococcus salivarius from the Human Oral Cavity. Dwight F. Gor-don, Jr........................................................... 1281

Identification of Actinomyces israelii and Actinomyces naeslundii by Fluorescent-Antibodyand Agar-Gel Diffusion Techniques. Frank W. Lambert, Jr., June M. Brown, andLucille K. Georg ............................................................ 1287

Media for the Enhancement of Fluorescent Pigment Production by Pseudomonas Species.J. A. Garibaldi........................................................... 1296

x

Cultural Characteristics and Fatty Acid Composition of Coryniebacterium acnes. C. WayneMoss, V. R. Dowell, Jr., V. J. Lewis, and M. A. Schekter......................... 1300

Presence of Rhapidosomes in Various Species of Bacteria and Their Morphological Charac-teristics. T. Yamamoto........................................................ 1746

Ultrastructural Studies of Microconidium Formation in Neurospora crassa. Robert J. Lowry,Thomas L. Durkee, and Alfred S. Sussman...................................... 1757

Nutrition and Metabolism of Marine Bacteria. XVI. Formation of Protoplasts, Sphero-plasts, and Related Forms from a Gram-negative Marine Bacterium. J. W. Costerton,Cecil Forsberg, Tibor I. Matula, F. L. A. Buckmire, and Robert A. MacLeod........ 1764

Ordered Substructure in the Cell Wall of Bacillus cereus. D. J. Ellar and D. G. Lundgren ... 1778Adherence of Mycoplasmas to Glass and Plastic. David Taylor-Robinson and Richard J.

Manchee.................................................................... 1781

Infection and Immunity

Staphylococcal 13-Hemolysin. II. Phospholipase C Activity of Purified 3-Hemolysin. S. K.Maheswaran and Robert K. Lindorfer.......................................... 1313

Biologically Active Endotoxins from Salmonella Mutants Deficient in 0- and R-Polysac-charides and Heptose. Yoon Berm Kim and Dennis W. Watson.................... 1320

Purification and Properties of Staphylococcal 5-Hemolysin. I. Production of 5-Hemolysin.Richard A. Murphy and Riaz-ul Haque......................................... 1327

Experimental Infection of the Cotton Rat (Sigmodon hispidus) with Rickettsia rickettsii. A.Shirai, F. M. Bozeman, J. W. Humphries, B. L. Elisberg, and J. E. Faber............ 1334

Experimental Transmission of a Murine Microsporidian in Swiss Mice. John B. Nelson .... 1340Degradation of Streptococcal Cell Wall Antigens In Vivo. John H. Schwab and S. H. Ohan-

ian..................................................................... 1346In Vivo and In Vitro Action of Norethindrone on Staphylococci. William W. Yotis........ 1353Development of Immune Tolerance in the Chick Embryo to Borrelia hispanica. Albert W.

Hanson and George R. Cannefax.............................................. 1359Lipopolysaccharides of Salmonella T Mutants. R. W. Wheat, M. Berst, E. Ruschmann, 0.

Luderitz, and 0. Westphal.................................................... 1366Cell Walls of Group D Streptococci. II. Chemical Studies on the Type 1 Antigen Purified

from the Autolytic Digest of Cell Walls. Arnold S. Bleiweis, Frank E. Young, andRichard M. Krause........................................................... 1381

Complement and Lysozyme Requirements for Spirochetolysis in Guinea Pig Serum. T. A.Nevin and W. J. Guest........................................................ 1388

Experimental Irradiated Arthrospore Vaccine Against Coccidioidomycosis in Mice. JamesD. Pulliam, J. L. Converse, E. M. Snyder, J. R. Esterly, and E. P. Lowe............ 1394

Serological Comparison of Spherules and Arthrospores of Coccidioides immitis. MarshallE. Landay, Robert W. Wheat, Norman F. Conant, and Edwin P. Lowe.............. 1400

Physicochemical Properties of the Vi Antigen Before and After Mild Alkaline Hydrolysis.Francis G. Jarvis, Marianne T. Mesenko, Donald G. Martin, and Theodore D. Per-rine..................................................................... 1406

Physicochemical and Biological Properties of Sonically Treated Vi Antigen. Donald G.Martin, Francis G. Jarvis, and Kelsey C. Milner................................. 1411

Role of the Phagocyte in Host-Parasite Interactions. XI. Relationship Between StimulatedOxidative Metabolism and Hydrogen Peroxide Formation, and Intracellular Killing.R. J. McRipley and A. J. Sbarra............................................... 1417

Role of the Phagocyte in Host-Parasite Interactions. XII. Hydrogen Peroxide-Myeloperoxi-dase Bactericidal System in the Phagocyte. R. J. McRipley and A. J. Sbarra ......... 1425

Avirulent Clostridium perfringens Strains Obtained by Euflavine Treatment. G. Paquetteand V. Fredette.............................................................. 1437

Antibiotic Susceptibility Patterns as Aids in Classification and Characterization of Gram-negative Anaerobic Bacilli. Sydney M. Finegold, Noriko E. Harada, and LawrenceG. Miller.................................................................... 1443

Characterization of Mycoplasma Strains from Cats. B. C. Cole, L. Golightly, and J. R.Ward..................................................................... 1451

Enhancement of Agrobacterium tumefaciens Infectivity by Mitomycin C. Gary T. Heber-lein and James A. Lippincott.................................................. 1470

CONTENTS Xi

CONTENTS

Cryptococcus neoformans.I. Nonencapsulated Mutants. G. S. Bulmer, M. D. Sans, and C.M. Gunn.................................................................... 1475

Cryptococcus neoformans. II. Phagocytosis by Human Leukocytes. G. S. Bulmer and M. D.Sans...................................................................... 1480

Leukocytic Hypersensitivity in Experimental Group A Streptococcal Infections. H. S. Lauand M. Scherago.............................................................. 1722

Association of Experimental Chronic Arthritis with the Persistence of Group A Strepto-coccal Cell Walls in the Articular Tissue. John H. Schwab, William J. Cromartie, Sar-kis H. Ohanian, and John G. Craddock......................................... 1728

Effects of Oil-treated Mycobacterial Cell Walls on the Organs of Mice. William R. Barclay,Robert Anacker, Werner Brehmer, and Edgar Ribi............................... 1736

Influence of Erythrocyte Concentration and Cation Levels on the Hemolytic Action of Staph-ylococcal a-Hemolysin. R. C. A. Sengers........................................ 1791

Response of the Vaccine Strain of Brucella melitensis Rev I to Erythritol. Dorothy Crouchand Sanford S. Elberg........................................................ 1793

Replacement of Ascitic Fluid or Rabbit Serum Requirement of Treponema dentium by a-Globulin. S. S. Socransky and C. Hubersak...................................... 1795

Relationship of Mycoplasma to Acute Leukemia in Children. H. Lazarus, K. M. K. Beckett,D. Cuppels, and G. E. Foley.................................................. 1797

Genetics and Molecular Biology

Genetic and Biochemical Studies on Mannose-negative Mutants That Are Deficient inPhosphomannose Isomerase in Escherichia coli K-12. Alvin Markovitz, Robert J.Sydiskis, and Michael M. Lieberman........................................... 1492

Derepression of Phosphomannose Isomerase by Regulator Gene Mutations Involved inCapsular Polysaccharide Synthesis in Escherichia coli K-12. Alvin Markovitz, MichaelM. Lieberman, and Nancy Rosenbaum......................................... 1497

Dominant Mutation for Ethionine Resistance in Saccharomyces cerevisiae. K. D. Spence,L. W. Parks, and S. K. Shapiro................................................ 1531

Deoxyribonucleic Acid Repair Replication After Ultraviolet Light or X-Ray Exposure ofBacteria. D. Billen, R. R. Hewitt, T. Lapthisophon, and P. M. Achey............... 1538

Thymidine and Thymine Incorporation into Deoxyribonucleic Acid: Inhibition and Repres-sion by Uridine of Thymidine Phosphorylase of Escherichia coli. Daniel R. Budmanand Arthur B. Pardee.................. ........................................ 1546

Role of a Cell Wall Glucan-degrading Enzyme in Mating of Schizopihyll/um commune. JosephG. H. Wessels and Donald J. Niederpruem...................................... 1594

Characterization of a Temperature-sensitive Mutant of Bacillus subtilis Defective in Deoxy-ribonucleic Acid Replication. Neil H. Mendelson and Julian D. Gross.............. 1603

Unusual Growth Characteristics of a Methionine-Cyano-B12 Auxotroph of Escherichia coli.Herbert Dickerman, Robert T. Taylor, and Herbert Weissbach.................... 1609

Occurrence of Chloramphenicol-acetylating Enzymes in Various Gram-negative Bacilli.Suyehiko Okamoto, Yoshiaki Suzuki, Katsutoshi Mise, and Rintaro Nakaya........ 1616

An Upper Limit on 3-Galactosidase Transfer in Bacterial Conjugation. J. L. Rosner, Ed-ward A. Adelberg, and Michael B. Yarmolinsky.................................. 1623

Conjugation in Escherichia coli: Recombination Events in Terminal Regions of Trans-ferred Deoxyribonucleic Acid. J. Pittard and E. M. Walker........................ 1656

Induction of Excessive Deoxyribonucleic Acid Synthesis in Escherichia coli by Nalidixic Acid.James V. Boyle, William A. Goss, and Thomas M. Cook.......................... 1664

Polyamines and the Accumulation of Ribonucleic Acid in Some Polyauxotrophic Strains ofEscherichia coli. Aarne Raina, Miekie Jansen, and Seymour S. Cohen............... 1684

Macromolecule Synthesis in Yeast Spheroplasts. H. Terry Hutchison and Leland H. Hart-well ........................................................................ 1697

Aromatic Amino Acid Biosynthesis: Gene-Enzyme Relationships in Bacillus subtilis. DeLillNasser and E. W. Nester...................................................... 1706

Nonselective Incorporation into Sporangium of Either "Older" or "Younger" Chromosomeof the Vegatative Cell During Sporulation in Bacillus cereus. Tokio Kogoma and Tomo-michi Yanagita.............................................................. 1715

xii

CONTENTS

Epistasis Between Deletion Mutations in the Tryptophan Region and Mutations Affectingthe General Aromatic Pathway in Escherichia coli K-12. Ronald L. Somervill. 1798

Ribosomes and Ribosomal Proteins from Neurospora crassa. II. Ribosomal Proteins in Dif-ferent Wild-Type Strains and During Various Stages of Development. Henry Roths-child, Hiraku Itakawa, and S. R. Suskind.1800

Induction of Mutations by Nitrous Acid on Denatured Haemophilus influenzae Deoxyribo-nucleic Acid Assayed Directly by Single-Stranded Transformation. Edith H. Posteland Sol H. Goodgal.1802

Selection of Thymine-requiring Strains from Escherichia coli on Solid Medium. John H.Caster.1804

Extraction and Characterization of Deoxyribonucleic Acid from Spores and VegetativeCells of Bacillus stearothermophilus. L. Tabatabai and H. W. Walker.1805

Microbial Physiology and Metabolism

Mechanisms of Protection of Trehalase Against Heat Inactivation in Neurospora. Shih-anYu, Alfred S. Sussman, and Susan Wooley.1306

Inhibition of Flagellar Coordination in Spirillum volutans. Noel R. Krieg, Joseph P. Tom-elty, and J. Scott Wells, Jr.1431

Energies of Activation and Uncoupled Growth in Streptococcus faecalis and Zymomonasmobilis. W. W. Forrest.1459

Synchrony of Nuclear Replication in Individual Hyphae of Aspergillus nidulans. R. F.Rosenberger and M. Kessel.1464

Catabolite Repression in the Facultative Chemoautotroph Thiobacillus novellus. H. B.LeJohn, L. van Caeseele, and H. Lees.1484

Penicillin Acyltransferase in Penicillium chrysogenum. David L. Pruess and Marvin J.Johnson.1502

Effect of Some Proteins on the Yeast Cell Membrane. D. A. Yphantis, J. L. Dainko, andF. Schlenk.1509

Urea Production and Putrescine Biosynthesis by Escherichia coli. David R. Morris andKara L. Koffron.1516

Carbohydrate Metabolism in Streptomycetes. II. Isolation and Enzymatic Synthesis ofTrehalose. Alan D. Elbein.1520

Autolytic Enzyme System of Streptococcus faecalis. III. Localization of the Autolysin atthe Sites of Cell Wall Synthesis. Gerald D. Shockman, Harold M. Pooley, and J.Stuart Thompson.1525

Ribosyl and Deoxyribosyl Transfer by Bacterial Enzyme Systems. Akira Imada and SeiziIgarasi.1551

Formation of a-Hydroxyglutaric Acid by Aspergillus glaucus. A. S. Bleiweis, H. C. Reeves,and S. J. Ajl.1560

Neuraminidase of Corynebacterium diphtheriae. Takafumi Moriyama and Lane Barksdale.. 1565Comparative Control of a Branch-Point Enzyme in Microorganisms. Roy A. Jensen, DeLill

S. Nasser, and Eugene W. Nester............................................... 1582Multiple Alterations in Metabolite Uptake in a Mutant of Neurospora crassa. Michael S.

Kappy and Robert L. Metzenberg.............................................. 1629Inducible Synthesis of Bacterial Luciferase: Specificity and Kinetics of Induction. John J.

Coffey ...................................................................... 1638Cytochrome Synthesis and Its Regulation in Spirillum itersonii. G. D. Clark-Walker, B.

Rittenberg, and J. Lascelles.................................................... 1648Induction of Penicillinase with Inorganic Phosphate. John Imsande and M. J. Gerber ... 1672Phospholipids of Thiobacillus thiooxidans. J. M. Shively and A. A. Benson.............. 1679Biosynthesis of the N-Acyl-Galactosamine in Cell Wall of Bacillus subtilis. F. E. Youngand L. Arias................................................................... 1783

Cytochromes of Pseudomonas syringae. David C. Sands, Frank H. Gleason, and D. C.Hildebrand................................................................. 1785

Presence of Chitin in the Cell Wall of a Griseofulvin-producing Species of Penicillium.D. A. Applegarth and G. Bozoian.............................................. 1787

Adenine and 2, 6-Diaminopurine as Germinants for Bacillus macerans Spores. L. E. Sacks. 1789

.x. .

CONTENTS

No. 6, DECEMBER 1967

Taxonomy, Ecology, Morphology and Structure, and Microbiological Methods

Identification of Mycoplasma and Other Microorganisms by Polyacrylamide-Gel Electro-phoresis of Cell Proteins. Shmuel Razin and Shlomo Rottem...................... 1807

Localization of Indigenous Yeast in the Murine Stomach. Dwayne C. Savage and ReneJ. Dubos.................................................................... 1811

Numerical Data Concerning the Sensitivity of Anaerobic Bacteria to Oxygen. V. Fredette,C. Plante, and A. Roy........................................................ 2012

Streptomyces steffisburgensis sp.n. Alma Dietz....................................... 2022Development and Structure of Bacterial Leaf Nodules in Psychotria bacteriophila Val.

(Rubiaceae). Nels R. Lersten and Harry T. Horner, Jr............................. 2027Cell Wall Composition in Relation to the Taxonomy of Some Actinoplanaceae. Paul J.

Szaniszlo and Harry Gooder................................................... 2037Microtubule in Azotobacter vinelandii Strain 0. Leodocia M. Pope and Peter Jurtshuk.... 2062

Infection and ImmunityInfluence of Carbon Tetrachloride on Inducible Liver Enzymes and Response to Endotoxin

in Mice. Irvin S. Snyder, Manjul K. Agarwal, and L. Joe Berry.................... 1817Endotoxic Glycolipid from a Heptoseless Mutant of Salmonella minnesota. N. Kasai and

A. Nowotny................................................................. 1824Identification of a Fourth Staphylococcal Enterotoxin, Enterotoxin D. E. P. Casman,

R. W. Bennett, A. E. Dorsey, and J. A. Issa..................................... 1875Fine Structure and Location of the Mycoplasma-like Gray Lung and Rat Pneumonia

Agents in Infected Mouse Lung. Frederick W. Gay............................ 2048Immunoelectrophoretic Analysis of the Antigenic Components of Bacillus megaterium.

P. Mastroeni, A. Nacci, and A. Rocca.......................................... 2073

Genetics and Molecular Biology

Mode of Action of Myxin on Escherichia coli. S. M. Lesley and R. M. Behki............ 1837Ribonucleic Acid Synthesis during Morphogenesis in Myxococcus xanithus. Karen Bacen

and Eugene Rosenberg........................................................ 1883O-Methylthreonine Inhibition of Growth and of Threonine Deaminase in Escherichia coli.

Mark E. Smulson, Marco Rabinovitz, and T. R. Breitman....................... 1890Organization of the Tryptophan Pathway: a Phylogenetic Study of the Fungi. R. Hutter

and J. A. DeMoss............................................................ 1896Analysis of Sporulation Mutants. I. Response of Uracil Incorporation to Carbon Sources

and Other Mutant Properties. Ernst Freese and Peter Fortnagel................... 1957Cold-sensitive Mutation of Pseudomonas putida Affecting Enzyme Synthesis at Low Tem-

perature. Seamus Condon and John L. Ingraham................................ 1970Dominance Studies with Stable Merodiploids in the D-Serine Deaminase System of Es-

cherichia coli K-12. Elizabeth McFall........................................... 1982"Position Effect" on Dominance in the D-Serine Deaminase System of Escherichia coli K-12.

Elizabeth McFall............................................................. 1989Characterization of Deoxyribonucleic Acids from Streptomycetes and Nocardiae. Enayat

M. Tewfik and S. G. Bradley.................................................. 1994Transient Repression of the lac Operon. Bonnie Tyler, William F. Loomis, Jr., and Boris

Magasanik.................................................................. 2001Somatic Antigen 2 Inheritance in Salmonella Groups B and D. E. M. Johnson............ 2018Biotin-requiring Mutants of Escherichia coli K-12. Alice del Campillo-Campbell, Gary

Kayajanian, Allan Campbell, and Sankar Adhya................................ 2065Effect of 6-Azathymine on the Isolation and Properties of Deoxyribonucleic Acid from

Streptococcus faecalis. Helge L. Gunther and William H. Prusoff.................. 2067R Factor Infection of Escherichia coli Lyophilized in 1946. David H. Smith............. 2071

Microbial Physiology and Metabolism

Biosynthesis of Biotin in Microorganisms. V. Control of Vitamer Production. JeromeBirnbaum, Chik H. Pai, and Herman C. Lichstein.............................. 1846

xiv

CONTENTS

Extraction, Characterization, and Cellular Localization of the Lipids of Staphylococcusaureus. David C. White and Frank E. Frerman................................. 1854

Membrane Lipid Changes During Formation of a Functional Electron Transport Systemin Staphylococcus aureus. Frank E. Frerman and David C. White................. 1868

Biotin-deficient Growth of Bacillus polymyxa. Jesse W. Summers and Orville Wyss....... 1908Tracer Studies to Locate the Site of Platinum Ions within Filamentous and Inhibited Cells

of Escherichia coli. E. Renshaw and A. J. Thomson..................... ..........1915Effect of Substrate on the Fatty Acid Composition of Hydrocarbon-utilizing Microorgan-

isms. K. R. Dunlap and J. J. Perry........................................... 1919Effect of Nutrients on Physiological Properties of Clostridium botulinum Type E. B. Gullmar

and N. Molin................................................................ 1924Biosynthesis of Biotin in Microorganisms. VI. Further Evidence for Desthiobiotin as a

Precursor in Escherichia coli. Chik H. Pai and Herman C. Lichstein................ 1930Release of Surface Enzymes in Enterobacteriaceae by Osmotic Shock. Harold C. Neu and

James Chou................................................................. 1934Recovery of Division Ability in Ultraviolet-irradiated Escherichia coli Induced by Photo-

reactivation, Photoprotection, and Liquid Holding Treatment. George J. Kantor andR. A. Deering............................................................... 1946

Control of Malate Synthase Formation in Rhizopus nigricans. Warner S. Wegener, Jane E.Schell, and Antonio H. Romano............................................... 1951

Thiobacillus thiooxidans Cell Wall Amino Acids and Monosaccharides. Edward H. Crumand Donald J. Siehr.......................................................... 2069

Dipicolinic Acid-less Mutants of Bacillus cereus. John Wise, Alice Swanson, and H. OrinHalvorson................................................................... 2075

Aging of Pseudomonas aeruginosa. C. E. Clifton.................................... 2077Amino Acid Composition of Cell Wall and Spore Coat of Bacillus subtilis in Relation to

Mycobacillin Production. Pinakilal Bhattacharyya and S. K. Bose................ 2079Author Index ..................................................................... 2081Subject Index ..................................................................... 2085Index to Date of Issue.............................................................. 2093

xv

40 JOURNAL OF BACTERIOLOGY

INDEX TO ADVERTISERS*AMBER LABORATORIES.................... 38

*AMERICAN STERILIZER CO.................... 16*BBL, DIVISION OF BIoQuEsT.................... 29*BELLCO GLASS INC......................................... 12

*BIOQUEST............................................... 9, 10

BLACKWELL SCIENTIFIC PUBLICATIONS, LTD..................... 34*BRISTOL LABORATORIES, INC............................... 14,15COLE-PARMER INSTRUMENT AND EQUiPMENT CO................. 30*DIFCo LABORATORIES................................... Cover 4

*EBERBACH CORPORATION................................... 38

*FALCON PLASTICS, DIV. OF BIOQUEST .......................... 21*FISHER SCIENTIFIC CO.............................. 37*GILFORD INSTRUMENT LABORATORIES, INC................. Cover 3

*INFORMATION RETRIEVAL, LTD............................. 19, 20*INTERNATIONAL EQUiPMENT CO............................... 18

*LAB-TEK PRODUCTS, DIV. OF MILES LABORATORIES, INC.39*E. LEITZ, INC................................................ 8*MILES LABORATORIES, INC................................1...I*NATIONAL APPLIANCE CO. 26*NATIONAL DRUG CO. 30

NATIONAL INSTITUTES OF HEALTH. 30NATIONAL INSTRUMENT LABORATORIES, INC. 36*NUTRITIONAL BIOCHEMICALS CORP... . .

Cover 2

OXOID, LTD.31RCA SCIENTIFIC INSTRUMENTS................................ 27

*SCIENTIFIC PRODUCTS, DIV. OF AMERICAN HOSPITAL SUPPLY CORP.33*SHEFFIELD CHEMICAL, DIVISION NATIONAL DAIRY PRODUCTS

CORPORATION.35*IVAN SORVALL, INC......................................... 25

SPRINGER-VERLAG NEW YORK, INC.32TRADERS PROTEIN DIVISION OF TRADERS OIL MILL Co.17*A.RTHUR H. THOMAS.28

*WARNER-CHILCOTT ................................ 13, 22, 23, 24

*Sustaining Member, American Society for Microbiology