Text of DNA Synthesis Eukaryotes DNA unwinds at a replication origin Two replication forks form...
DNA Synthesis Eukaryotes DNA unwinds at a replication origin Two replication forks form Bidirectional synthesis of DNA Much more DNA; complexed with proteins Eukaryotes have much more DNA Yeast 250-400 replicons Mammalian cells up to 25,000 Solution: Multiple replication origins Yeast 4X that of E. coli Drosophila 100X that of E. coli
DNA Synthesis in Eukaryotes Pre-initiation replication complex: ORC Cdt1 Cdc6 MCM
Enzyme FunctionProkaryotesEukaryotes Helicase Primase Polymerase Proofreading Sliding clamp Prevent ss closure DnaB DnaG Pol III sliding clamp SSBPs MCM Primase s.u. of -polymerase Pol and PCNA (Proliferating Cell Nuclear Antigen) RPA (Replication Protein A) Comparison of some replication components