Particle Characterization

Proteomics

Genomics

Cell Analysis

Centrifugation

Lab Tools

Bioseparation

Lab Automation

Your solution for nanoparticle sizing and

zeta potential measurements.

DelsaNano Series – S, C, Z, HC

BR-11313B

DelsaNano – The Solution to Your Nanoparticle Size

and Zeta Potential Analysis Needs

Designed with the user inmind – easy and simple,yet so complete.

The DelsaNano utilizes Photon Correlation Spectros-copy and Electrophoretic Light Scattering techniquesto determine particle size and zeta potentialof materials including flat surfaces respectively.Offering an excellent degree of accuracy, resolutionand reproducibility, the DelsaNano has been

designed to simplify your submicron particle sizeand zeta potential analyses.

Whether in a QC environment or research anddevelopment, the DelsaNano provides the solutionsnecessary to obtain results fast and accurately.

Patented Forward Scatteringthrough Transparent Electrode(FST) method

Unique flat surface cell will allowthe measurement of zeta potentialof flat surfaces

The highly sensitive DelsaNano HCwill allow the size measurementof particles that are weakscatterers

Determine electrophoretic mobilityand zeta potential of concentratedsamples

Evaluate surface charge of solidflat surfaces by electrophoreticlight scattering

Determine size and distributionof particles that scatter lightweakly

DelsaNano Series –

Power, Flexibility and Simplicity

DelsaNano S

• Offers accurate, reliable and repeatable particlesize analysis, time after time

• Size range from 0.6 nm to 7 µm

• Sample concentration ranging from 0.001% to 40%(sample dependent)

• Analysis of aqueous and non-aqueous samples

Where to apply all these features?

� Nanoparticles

� Pharmaceuticals

� Inks

DelsaNano C

Offers particle size and zeta potential measurementswith high accuracy and resolution.

• Dual 30 mW laser• Size range from 0.6 nm to 7 µm

• Size range for zeta potential from 0.6nm to 30µm

• Sample concentration ranging from 0.001% to 40%

• Zeta potential of flat surfaces

• Auto-titration available for suspension and flat samples

Where to apply all these features?

� Shelf life studies

� Pharmaceuticals

� Food & beverage

� Semiconductors

� Emulsion stability

� Textiles

� Formulation stability

� Biotechnology

DelsaNano Z

Offers accurate, repeatable zeta potential measurementof particles in suspension covering a wide sampleconcentration range.

• Size range for zeta potential: 0.6 nm to 30 µm

• Concentration range: from 0.001% to 40%(sample dependant)

• Performs zeta potential analysis of flat surfaces

• Auto-titration available for suspension samplesand flat samples

Where to apply all these features?

� Shelf-life studies

� Emulsion stability

� Food & beverage

� Textiles & fabrics

� Formulation stability

� Food & beverage

� Proteins

DelsaNano HC

Offers high sensitivity particle sizing and zeta potentialmeasurements with the highest degrees of accuracy,sensitivity and resolution regardless of sampleconcentration.

• Size range from 0.6 nm to 7 µm

• Size range for zeta potential from 0.6 nm to 30 µm

• Concentration range from 0.001% to 40%(sample dependant)

• Dual 30 mW laser

• Includes a higher sensitive PMT

• Auto-titration available for suspension samplesand flat samples

Where to apply all these features?

� Biological molecules

� Biotechnology

� Nanoparticles

Electrophoretic Light Scattering

Theory and Function

Photon Correlation Spectroscopy

Electrophoretic light scattering is the method mostpopularly used to determine the velocity of theparticles suspended in a fluid medium underan applied electric field. In order to determine thespeed of the particles movement, the particles areirradiated with a laser light and the scattered lightemitted from the particles is detected. Since thefrequency of the scattered light is shifted from theincident light in proportion to the speed of theparticles movement, the electrophoretic mobility

of the particles can be measured from the frequencyshift (Doppler shift) of the scattered light.

Measurement Process

When an electric field is applied to charged particlesin the suspension, particles move toward an electrodeopposite to their surface charge. Since the velocity isproportional to the amount of charge of the particles,zeta potential can be estimated by measuring thevelocity of the particles.

Photon correlation spectroscopy is a techniqueused to determine the diffusion coefficient of smallparticles in fluid. The coefficient is determinedby accurately measuring the light scattering intensityof the particles as a function of time.

Measurement Process

As the particles of interest diffuse within the samplecell due to Brownian motion, an incident beam oflaser light illuminates the particles. The particles scatterthe light, producing fluctuations in the scatteringintensity as a function of time. The scattered lightis collected at a chosen angle, and is measuredby a highly sensitive detector. Since the diffusion rate

of particles is determined by their size, informationabout their size is contained in the rate of fluctuationof the scattered light. So, by correlating the fluctuation,we can determine the particle size distribution of thepopulation present.

One of the Widest Concentration Ranges

The DelsaNano is capable of analyzing samples withconcentrations ranging from 0.001% to 40% (sampledependent), for both particle size and zeta potential.

Zeta Potential of Concentrated Samples

Electrophoretic light scattering measurementson concentrated samples are now available, thanksto the DelsaNano patented Forward Scatteringthrough Transparent Electrode Technology (FST).This method detects the scattered light from particlesthrough a transparent electrode at a 30° scatteringangle by using a short optical path that reduces effectsdue to multiple scattering with very high accuracy.

Major Features

Get a true zeta potentialwithout the need to diluteyour sample.

Zeta Potential of Flat samples

Novel method to measure the zeta potential of flatsurfaces like films, fibers, etc. using probing particles.

Advantages:

• Ability to measure the electrostatic interactionsbetween the particles and flat surface

• Two zeta potentials (particles and flat surface)in one click

• Zeta potential vs. pH/ Additive Volume alsoavailable

• Simple, easy and straight forward technique

Applications Include:

� Fibers and textiles

� Thin films

� Shampoo and conditionerindustry

� Membranes and filters

� Biological surfaces andbiomaterials

� Semiconductor industry

� Polymer surfaces andcoatings

� Optical glass polishing

� Protein adsorptionstudies

� Printing and paintindustry

� Paper and pulp industry

� Antimicrobial surfaces

� Packaging materials

� Recording media

� Leaves

Figure 1. Zeta potential of glass slide coated with PEI and PSSNapolyelectrolytes layer by layer (LBL) alternatively.

Analyze your results locally or remotely!

Standard Operating Procedures – SOP – Your SmartSolution for Consistent Results Run-after-Run

Software – Flexible and Easy to Use

Configurable Security System

Choose from three levels of security: no security,security or 21 CFR Part 11. By selecting the21 CFR Part 11 option, the system automaticallyreconfigures to comply with the FDA regulation.

Flexible Data Export and Import

Provides you with the flexibility to share yourresults with your colleagues. All data may beexported and later imported.

Editable Diluent Tables

• Manage your diluents with the editablediluents table

• Let the software do the work for you. Inputjust two values for each diluent parameterand the software will automatically determinethe rest for you.

• Perform analysis as easy as 1-2-3:

1. Load your sample

2. Create or select an SOP

3. Start your analysis and view your results

• Get accurate results time-after-time fromdifferent operators and/or instruments

Optional Cells

DelsaNano Series is compatible with a variety ofcells for measuring size and zeta potential of particlesin fluid suspensions including the zeta potential offlat samples. Below is table listing all cells and theirmajor benefits.

DelsaNano Accessories

Cell Function Major Benefits Part Number*

Micro Volume Cell (Quartz) Particle size in aqueoussuspensions & organic solvents

Handles low volume samples as lowas 30 µL A54094

Size Cell (Quartz) Particle size in aqueoussuspensions & organic solvents Reusable A54092

Disposable Size Cell Particle size in aqueoussuspensions Disposable A54093

Flow CellParticle size and zeta potential oflow concentrated aqueoussuspensions

Measures both size and zeta potential in thesame cell without the need to remove the sample.Compatible with DelsaNano auto-titrator

A54114

Disposable Zeta Cell Zeta potential of low concentratedaqueous suspensions

Disposable sample holderReusable electrodes A54118

High Concentration Cell Zeta potential of aqueoussuspensions

Handles high concentrated samples(up to 40% w/v & sample dependent) A54116

Flat Surface Cell Zeta potential of flat surfacesMeasures zeta potential of flat surfacesand particles in one click. Measures zetapotential vs. pH/Additive volume

A54117

Low Conductivity Cell Zeta potential in organic solventsSpecially designed for handling organicsolvent based samples. Easy to clean andreusable

A54119

* Cell assembly

Cell compatibility with DelsaNano models S C Z HC

Micro Volume Cell (Quartz) � � X �

Size Cell* (Quartz) � � X �

Disposable Size Cell � � X �

Flow Cell X � � �

Disposable Zeta Cell X � � �

High Concentration Cell X � � �

Flat Surface Cell X � � �

Low Conductivity Cell X � �

DelsaNano AT Autotitrator

The DelsaNano AT is an optional accessory usedwith the DelsaNano to titrate sample suspensionsin a pH range from 1 to 13. It automatically controlsthe pH of solutions and performs titrations duringzeta potential or size analysis measurements.

• Walk away operation

• Automatically determines iso-electric point

• All settings of the titration and measurementcontrolled by the software via standard operatingprocedures (SOP)

• Reports and graphs automatically generated

• Plot size and zeta potential vs. pH value andadditive volume

USA, Brea, CA (1) 800 352 3433, (1) 714 993 5321 Email pc-sales@coulter.comFor all other countries and specific phone numbers for your local distributor, please visit:www.CoulterCounter.com/contactB2009-10724-LC-2.5K www.beckmancoulter.com © 2009 Beckman Coulter, Inc. DIAG-PRINTED IN U.S.A.

Visit our online store at:www.beckmancoulter.com/eStore

For more information on our Particle Characterization products,please visit us at www.CoulterCounter.com

Printed with soy ink

Space forFSC Logo

Beckman Coulter, the stylized logo and COULTER COUNTER are registered trademarks ofBeckman Coulter, Inc. DelsaNano is a trademark of Beckman Coulter, Inc. Windows is a registeredtrademark of Microsoft Corporation in the United States and other countries.

Size measurement of particles influid suspensions

Size and zeta potential measure-ment of particles in fluid suspen-sions. Zeta potential measurementof flat surfaces

Zeta Potential measurement ofparticles in fluid suspensions.Zeta potential measurementof flat surfaces

High sensitivity sizing and zetapotential measurement of particlesin fluid suspensions. Zeta potentialmeasurement of flat surfaces

Photon correlationspectroscopy

Photon correlation spectroscopyand Electrophoretic light scattering

Electrophoretic lightscattering

Photon correlation spectroscopyand Electrophoretic light scattering

CONTIN, NNLS and Marquardtincluding Cumulants diameter

CONTIN, NNLS and Marquardtincluding Cumulants diameter

NA CONTIN, NNLS and Marquardtincluding Cumulants diameter

NA Smolochowski, Huckeland Other

Smolochowski, Huckeland Other

Smolochowski, Huckeland Other

Size: 0.6 nm-7 µm

Molecular Weight:103 - 3 × 107 Daltons

Size: 0.6 nm-7 µm

Molecular Weight:103 - 3 × 107 Daltons

Size Range for Zeta potential:0.6 nm - 30 µm

Electrophoretic Mobility:± 1.2 × 10-3 cm2/V.s

Zeta Potential: ± 150 mV

Size Range for Zeta potential:0.6 nm - 30 µm

Electrophoretic Mobility:± 1.2 × 10-3 cm2/V.s

Zeta Potential: ± 150 mV

Size: 0.6 nm-7 µm

Molecular Weight:103 - 3 × 107 Daltons

Size Range for Zeta potential:0.6 nm - 30 µm

Electrophoretic Mobility:± 1.2 × 10-3 cm2/V.s

Zeta Potential: ± 150 mV

0.001%-40% (w/v) 0.001%-40% (w/v) 0.001%-40% (w/v) 0.001%-40% (w/v)

NA Up to 200 mS/cm Up to 200 mS/cm Up to 200 mS/cm

Size: 30 µL Size: 30 µLZeta Potential: 0.7 mL

Zeta Potential: 0.7 mL Size: 30 µLZeta Potential: 0.7 mL

NA 0-300 V; Auto or Fixed 0-300 V; Auto or Fixed 0-300 V; Auto or Fixed

Dual; Time domain and time ofarrival; Maximum of 1,000,000equivalent channels

Dual; Time domain and time ofarrival; Maximum of 1,000,000equivalent channels

Dual; Time domain and time ofarrival; Maximum of 1,000,000equivalent channels

Dual; Time domain and time ofarrival; Maximum of 1,000,000equivalent channels

Size: 165 Size: 15º, 165

Zeta Potential: 15º, 30

Zeta Potential: 15º, 30 Size: 15º, 165

Zeta Potential: 15º, 30

Single 30 mW laser diode Dual 30 mW laser diodes Single 30mW laser diode Dual 30 mW laser diodes

PMT PMT PMT PMT

15°C ± 0.3°C below ambient to

90°C ± 0.3°C to 90°C ± 0.3°C

15°C ± 0.3°C below ambient to

90°C ± 0.3°C to 90°C ± 0.3°C

15°C ± 0.3°C below ambient to

90°C ± 0.3°C to 90°C ± 0.3°C

15°C ± 0.3°C below ambient to

90°C ± 0.3°C to 90°C ± 0.3°C

380 mm x 550 mm x 212 mm(15 in x 21.7 in x 8.35 in)

380 mm x 550 mm x 212 mm(15 in x 21.7 in x 8.35 in)

380 mm x 550 mm x 212 mm(15 in x 21.7 in x 8.35 in)

380 mm x 550 mm x 212 mm(15 in x 21.7 in x 8.35 in)

20 kg (44.1 lb) 21 kg (46.3 lb) 21 kg (46.3 lb) 21 kg (46.3 lb)

Function

Principle

Size DistributionMethods

Zeta PotentialCalculation Methods

MeasurementRange

Sample Concentration*

Conductivity

Minimum SampleVolume

Voltage Range & Mode

Correlator

Scattering Angles

Light Source

Detector

Temperature Range

Dimensions (W x D x H)

Weight

Number of Titrants Maximum of three

Titrant Volume Standard glass vials: 50 mLPlastic vials: 50 mL

Sample Volume Minimum: 30 mLMaximum: less than 50 mLfor standard vial

Titrant Dispense Minimum: 0.1 µLVolume

Circulation Flow Rate 10 - 40 mL/min

Maximum Number 100of pH Settings

pH Probe Fast response liquid filled glasscombination electrode

pH Range 1 - 13

pH Calibration User defineable up to threepoints

Sample Stirrer Magnetic

Dimensions 250 mm x 310 mm x 290 mm(W x D x H) 9.8 in x 12.2 in x 11.4 in

Weight 7.5 kg (16.5 lb)

DelsaNano S DelsaNano C DelsaNano Z DelsaNano HC DelsaNano AT

*Sample dependent