1Deadline = line + dead

deadline |ˈdedˌlīn|noun1 this definition not relevant2 historical a line drawn around a prison beyond which prisoners were liable to be shot.

think about it

Does it really make sense to designate that period at the end as the target as opposed to that at the

beginning?

Is it really the case that your schedule is such that all available times just happen to fall at the last minute?

22

Beating TamifluBeating TamifluThemes:

Evolution: then and nowamino acids, mutations, folding: they

matter

Themes: Evolution: then and now

amino acids, mutations, folding: they matter

3How evolution happens

• Good ideas are rewarded not sought/planned

• There’s no mechanism for foresight, so it cannot be that way

4Just passin’ through

http://www.ch.ic.ac.uk/local/projects/sanderson/images/lifecyc.gif

5Tamiflu: you shall not pass!

http://en.wikipedia.org/wiki/File:Oseltamivir-3D-balls.png

a.k.a. oseltamivir

6And it goes like this...

http://www.ks.uiuc.edu/Research/influenza/

Note: this is modeled, not a crystal structure

7Cell sorting

• Laser is like a black light--molecules GLOW a different color than they are being hit with

• In our case, GFP (green) and cherry (you guess it)

8Phylogenetic trees

• Participants: to your spot on the tape!

• Observers: watch & predict outcome and what it’s good for

Each round:Copy any changes your parent already hasDetermine position of your new change by adding #s (0 = none)Randomly change to new base & mark (A, G, C, U)Turn to your kids & repeat

9Supplemental note

• What we did was just the tip of the iceberg

• among other things, the same basic thinking can be done to reconstruct a tree even if we only have the current [last] generation

• try putting your thumb over any individual and seeing if you can guess their sequence by looking at their children

1010

SupplementalSupplementalStuff you might find usefulStuff you might find useful

11Supplemental

• Cartoon of infections & tamiflu action (contr. Katie F12)

• http://www.youtube.com/watch?v=0qCTyKrhVWc

1212

UnusedUnusedThis was a graphical presentation of

what we ended up doing ‘live’ in class. It tries to make the same points: the

‘correct’ reconstruction has fewer total changes (parent-child sequence alterations) than a random one

This was a graphical presentation of what we ended up doing ‘live’ in class. It

tries to make the same points: the ‘correct’ reconstruction has fewer total

changes (parent-child sequence alterations) than a random one

13

14Now what???

What information allows you to make good inferences about who is most related?

Who gave rise to whom?

15

Which makes sense?

Or this?Or this?Or this?Or this?

16How about this?