MM Caffarel et al. Supplemental Figure 1

A

510 bp (JAK2 ) 408 bp (JAK2 )

5 d 10 d 15 d 72 h 96 h IEC 4 w 8 w Cre-

virgin gestation involution

B

C

JAK2 mutant Control

4 w

eek

old

8 w

eek

old

JAK2 mutant Control

nuclei CK18 CK14

Figure S1. Characterisation of K14-Cre;JAK2 V617F mice. (A) Representative PCR gel showing levels of recombination in the whole mammary gland of K14-Cre;JAK2 V617F mice at the indicated time points and in isolated epithelial cells (IEC) from a K14-Cre;JAK2 V617F mammary gland at 10 days gestation. w, d, h represent weeks, days and hours respectively. Lactation sample was collected at first day post-partum. JAK2R and JAK2F are the recombined and the targeted allele respectively. A lane showing no recombination in a JAK2 V617F mammary gland has been included as a control. (B) Representative whole-mount pictures of the abdominal mammary gland of a 4 (top) and 8 (bottom) week old virgin JAK2 V617F (control, left) or K14-Cre;JAK2 V617F (right) female. (C) Representative immunofluorescence staining for cytokeratin -14 (CK14, in red) and -18 (CK18, in green) in the abdominal mammary glands of JAK2 V617F (control, left) or 8 week virgin K14-Cre;JAK2 V617F (right) females. Nuclei are in blue. Scale bars: 1 mm (B) and 20 µm (C).