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WB: β Actin. Control Infected. Control Infected. Control Infected. 1. 1. NS1 siRNA. M1 siRNA. M2 siRNA. 2. 2. WB: NP. WB: M2. WB: NS1. WB: M1. B. A. WB: β Actin. WB: β Actin. WB: β Actin. 1. 1. 2. 2. D. C. Control siRNA. Control Infected. NP siRNA. Fig. S1. - PowerPoint PPT Presentation
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Contro
l Inf
ecte
dNS1
siRNA
WB: NS1
WB: β Actin
Contro
l Inf
ecte
dM
1 siR
NA
WB: M1
WB: β Actin
Contro
l Inf
ecte
dCon
trol s
iRNA
NP siR
NA
WB: NP
WB: β Actin
Fig. S1
A B
C
1
2
1
2
1
2
Contro
l Inf
ecte
dM
2 siR
NA
WB: M2
WB: β Actin
1
2
D
pEGFP
pEGFP-NS1
pEGFP-M2
pEGFP-M1
pEGFP-NP
Control
Fig. S2
- + - + - + - +
- +
Fig. S3
PR8 IAV
IP anti NP IP anti M1 IP anti M2 IP anti NS1
WB anti CLU
CLU
A
WB GAPDH
WB NP
WB NS1
WB CLU
WB M2
WB M110% Input
PR8 IAV
B
1
2
3
4
5
6
Fig. S4
SP β chain α chain
β chain α chainGST
β chainGST
α chainGST
Nascent Clusterin
CLU Full length
CLU β Chain
CLU α Chain
IAV NP 6xHis
A
Fig. S4
GST pull downWB: His
GST pull downWB: GST
GST Pull down samples
GST Pull down samples
1 2 3 4
1 2 3 4
WB: His
WB: GST
1% input
1% input
1 2 3 4
1 2 3 4
1. CLU Full- GST + IAV NP-His
2. CLU β-GST + IAV NP-His
3. CLU α-GST + IAV NP-His
4. GST + IAV NP –His
B C
1 1
22
Fig. S5
WB: NP
WB: Calnexin
WB: CLU
Mock 0h 4h 8h 12h
WB: GAPDH
1
2
3
4
BA
NP Cyt C
Hoechst Merge
pcDNA3.1 myc.
NP Cyt C
Hoechst Merge
pcDNA3.1 myc-NP
Fig. S6
Fig. S7
1
2
WB CLU
WB β Actin
Contro
l
pCM
VpC
MV X
L4- C
LU
A B
1
2
Contro
l
Contro
l siR
NACLU
siR
NA
WB CLU
WB β Actin