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Co ntr o l In f e c t ed NS 1 s i RNA WB: NS1 WB: β Actin Cont r o l In f e cted M1 s i R NA WB: M1 WB: β Actin Con tr o l In fec t ed Co n t r o l s i R N A NP si R NA WB: NP WB: β Actin Fig. S1 A B C 1 2 1 2 1 2 Co n trol In f e c ted M2 s i R NA WB: M2 WB: β Actin 1 2 D

Control Infected

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WB: β Actin. Control Infected. Control Infected. Control Infected. 1. 1. NS1 siRNA. M1 siRNA. M2 siRNA. 2. 2. WB: NP. WB: M2. WB: NS1. WB: M1. B. A. WB: β Actin. WB: β Actin. WB: β Actin. 1. 1. 2. 2. D. C. Control siRNA. Control Infected. NP siRNA. Fig. S1. - PowerPoint PPT Presentation

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Page 1: Control Infected

Contro

l Inf

ecte

dNS1

siRNA

WB: NS1

WB: β Actin

Contro

l Inf

ecte

dM

1 siR

NA

WB: M1

WB: β Actin

Contro

l Inf

ecte

dCon

trol s

iRNA

NP siR

NA

WB: NP

WB: β Actin

Fig. S1

A B

C

1

2

1

2

1

2

Contro

l Inf

ecte

dM

2 siR

NA

WB: M2

WB: β Actin

1

2

D

Page 2: Control Infected

pEGFP

pEGFP-NS1

pEGFP-M2

pEGFP-M1

pEGFP-NP

Control

Fig. S2

Page 3: Control Infected

- + - + - + - +

- +

Fig. S3

PR8 IAV

IP anti NP IP anti M1 IP anti M2 IP anti NS1

WB anti CLU

CLU

A

WB GAPDH

WB NP

WB NS1

WB CLU

WB M2

WB M110% Input

PR8 IAV

B

1

2

3

4

5

6

Page 4: Control Infected

Fig. S4

SP β chain α chain

β chain α chainGST

β chainGST

α chainGST

Nascent Clusterin

CLU Full length

CLU β Chain

CLU α Chain

IAV NP 6xHis

A

Page 5: Control Infected

Fig. S4

GST pull downWB: His

GST pull downWB: GST

GST Pull down samples

GST Pull down samples

1 2 3 4

1 2 3 4

WB: His

WB: GST

1% input

1% input

1 2 3 4

1 2 3 4

1. CLU Full- GST + IAV NP-His

2. CLU β-GST + IAV NP-His

3. CLU α-GST + IAV NP-His

4. GST + IAV NP –His

B C

1 1

22

Page 6: Control Infected

Fig. S5

WB: NP

WB: Calnexin

WB: CLU

Mock 0h 4h 8h 12h

WB: GAPDH

1

2

3

4

Page 7: Control Infected

BA

NP Cyt C

Hoechst Merge

pcDNA3.1 myc.

NP Cyt C

Hoechst Merge

pcDNA3.1 myc-NP

Fig. S6

Page 8: Control Infected

Fig. S7

1

2

WB CLU

WB β Actin

Contro

l

pCM

VpC

MV X

L4- C

LU

A B

1

2

Contro

l

Contro

l siR

NACLU

siR

NA

WB CLU

WB β Actin