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1 Copyright ©2011 MicroPhotoAcoustics, Inc. COMPANY PROFILE MicroPhotoAcoustics, Inc. (MPA)—a spin-off from Advanced Optowave Coporation—was founded in 2010 with the inventors of three-dimensional photoacoustic microscopy. Multiple patents have been licensed from Dr. Lihong Wang’s lab, Optical Imaging Laboratory at Washington University in St. Louis. MPA provides commerical photoacoustic microscopy (PAM) systems at both optical resolution (OR-PAM) and acoustic resolution (AR-PAM) worldwide. TECHNOLOGY OVERVIEW Photoacoustic microscopy is the fastest growing biomedical imaging technology, which affords high-resolution sensing of rich optical contrast in vivo at super-depths—depths beyond the optical transport mean free path (~1 mm in the skin). Although commercially available high-resolution three-dimensional optical imaging modalities—including confocal microscopy, two-photon microscopy and optical coherence tomography—have fundamentally impacted biomedicine, none can reach super-depths in tissue. In PAM, short-pulsed laser light is absorbed by biological tissue and converted to transient heating, which is subsequently converted into an ultrasonic wave due to thermoelastic expansion. Detection of the ultrasonic wave yields a tomographic image. Taking advantage of low ultrasonic scattering, PAM equivalently improves tissue optical transparency by a factor of 1000 and consequently enables super-depth penetration at high resolution. As a unique feature, PAM is exquisitely sensitive to optical absorption, most sensitive among all imaging modalities. PAM enables in vivo imaging of the smallest blood vessels (i.e., capillaries) and single cells with intrinsic tissue contrast (i.e., no exogenous contrast agent injected into the tissue). Both functional and molecular imaging based on optical contrast have been achieved by PAM. PHOTOACOUSTIC MICROSCOPY SYSTEM

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Page 1: COMPANY PROFILE TECHNOLOGY OVERVIEW - Photoacoustic Microscopybiompa.com/wp-content/uploads/2011/10/brochure-2012-03.pdf · COMPANY PROFILE. MicroPhotoAcoustics ... founded in 2010

1Copyright ©2011 MicroPhotoAcoustics, Inc.

COMPANY PROFILEMicroPhotoAcoustics, Inc. (MPA)—a spin-off from Advanced Optowave Coporation—was founded in 2010 with the inventors of three-dimensional photoacoustic microscopy. Multiple patents have been licensed from Dr. Lihong Wang’s lab, Optical Imaging Laboratory at Washington University in St. Louis. MPA provides commerical photoacoustic microscopy (PAM) systems at both optical resolution (OR-PAM) and acoustic resolution (AR-PAM) worldwide.

TECHNOLOGY OVERVIEWPhotoacoustic microscopy is the fastest growing biomedical imaging technology, which affords high-resolution sensing of rich optical contrast in vivo at super-depths—depths beyond the optical transport mean free path (~1 mm in the skin). Although commercially available high-resolution three-dimensional optical imaging modalities—including confocal microscopy, two-photon microscopy and optical coherence tomography—have fundamentally impacted biomedicine, none can reach super-depths in tissue. In PAM, short-pulsed laser light is absorbed by biological tissue and converted to transient heating, which is subsequently converted into an ultrasonic wave due to thermoelastic expansion. Detection of the ultrasonic wave yields a tomographic image. Taking advantage of low ultrasonic scattering, PAM equivalently improves tissue optical transparency by a factor of 1000 and consequently enables super-depth penetration at high resolution. As a unique feature, PAM is exquisitely sensitive to optical absorption, most sensitive among all imaging modalities. PAM enables in vivo imaging of the smallest blood vessels (i.e., capillaries) and single cells with intrinsic tissue contrast (i.e., no exogenous contrast agent injected into the tissue). Both functional and molecular imaging based on optical contrast have been achieved by PAM.

PHOTOACOUSTIC MICROSCOPY SYSTEM

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2Copyright ©2011 MicroPhotoAcoustics, Inc.

Comparison of Optical Microscopy Technologies

Imaging Capability Confocal Microscopy

Two-photon Microscopy

Optical Coherence

Tomography

Photoacoustic Microscopy

Depth 0.2 mm 0.5 mm 1 mm1 mm by OR-PAM3 mm by AR-PAM (50 MHz)

Contrast Fluorescence, scattering Fluorescence Scattering,

polarization Absorption

Resolution ~Depth/200 ~Depth/100 ~Depth/100 ~Depth/100

Multiscale imaging N N N Y

Sensitivity to absorption Low (6%) None Low (0.4%) High (100%)

Doppler sensing N N Y Y

Speckle artifact N N Y N

Oxygen saturation of hemoglobin N N N (poor

sensitivity) Y

Intrinsic-contrast melanin imaging N N N Y

Molecular imaging Y Y N (poor sensitivity) Y

Reporter gene imaging Y Y N Y

Imaging of metabolic rate of oxygen N N N Y

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SYSTEM LAYOUT

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APPLICATIONS

1) Highresolution(5μmlateralresolution)mousebrainimagingwithskullintact.

500 µm

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2) Capillaryandsingleredbloodcellimaging in a mouse ear in vivo.

RBC

Capillary bed

500 µm

50 µm

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0.4

sO2

1

3) Oxygenationsaturation.StructuralandfunctionalmicrovascularimagingofanudemouseearinvivobyOR-PAM.Upperleft:structuralimageacquiredat570nm;upperright:vessel-by-vesseloxygenationsaturation(sO2)mappingbasedondual-wave-lengthmeasurements.ThearteriesandveinscanbeidentifiedbasedonthesO2map.A1,arepresentativearteriole;V1,arepresentativevenule;lower:wholeearsO2map.

500 µm

100 µm

sO2

1

0

100 µm

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4) Contrastagents.IntravitalPAMofamyloidplaqueswithCongoRedDye.

Fluorescence

OR-PAM

TPM100 µm

100 µm

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5) Label-freePAMofocularmicrovasculatureinvivo.

200 µm

(µm)

xu.zhun
Pencil
xu.zhun
Pencil
xu.zhun
Pencil
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6) Tumorangiogenesismonitoring.Longitudinalmonitoringofdoxycycline-regulatedHIF-mediatedneovascularization ina mouseear.

Non-transgenic mouse Transgenic HIF mouse

500 µm

Stage 1: D

evelopment

Stage 2: M

aintenance

d0

d1

d3

d14

d30

d60

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7) Humanfingercuticle.

200 µm

500 µm

HighsO2

LowsO2

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(a)

(b)

(c)

8) Transversebloodflow.Simultaneousphotoacousticimagingofoxygensaturationandbloodflow.(a)MAPofstructuralimage.(b)MAPofoxygensaturationimagemeasuredbytwowavelengths560nmand570nm.(c)BloodflowimagemeasuredbytheDopplerbandwidthbroadening.Theflowdirectionwasdeterminedbyabidirectionalmotorscanning.(d)Thevasculartraceswiththeoxygensaturation,averagedalongthesegmentsofthevessels.(e)Thevasculartraceswiththebloodflowvelocity,inmillimeterspersecond,averagedalongthesegmentsofthevessels.(f)Thetransversevelocityprofileofthebloodflowindicatedbythedashedlinein(c).Scalebar=250μm.

Min

4

2.

0

-2

-4

1.0

0.9

0.8

0.7

0.6

velo

city

(mm

/s)

Flow

vel

ocity

(mm

/s)

Oxy

gen sa

tura

tion

Opt

ical

abs

orpt

ion

(d)

(e)

(f)

6

4

2

0

-2

-4

-60 200 400 600 800 1000

Position (µm)

M

ax

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9) Monitoringofmelanomagrowthonanudemouseear.(a)PAMimageofbloodveselsintheearacquiredbeforeinjectionofmelanomacells.(b)PAMimagewhereinvivoredbloodcells(RBCs)canbeidentified(theclose-upimageindicatesthebiconcavestructureofRBCs).(c)PAMimageofbloodvesselsandmelanomatakenfourdaysafterinjectingmelanomacells.ThemelanomaimageisextractedbytakingthedifferenceofthetwoPAMimages[(a)and(c)]andisplottedingray:MT,melanomatumor.(d)Opticalmicroscopyimage(0.057NA)ofthesameareain(a)and(c).

a) b)

c)

Vasculature

Melanoma

max

min

d)

200 µm 20 µm

5 µm

MT

RBC

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10) Imageofsmallintestine.

25 µm

25 µm

PAM image of section without staining.Section thickness: 6 µm.

Optical micrograph (20X, 0.45 N.A.) of the section stained by hematoxylin and eosin.

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OR-PAM Systems

In vivo imaging at tissue depths up to 1.2 mm

Lateral resolution down to 5 µm

Axial resolution down to 15 µm

Integrated, KHz tunable laser

AR-PAM Systems

In vivo imaging at tissue depths up to 3 mm

Axial resolution down to 15 µm

Lateral resolution down to 45 µm

Integrated, KHz tunable laser

TECHNICAL SPECIFICATIONS

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MicroPhotoAcoustics Inc113 Comac Street

Ronkonkoma, NY 11779, USA

Tel : 1-631-974-6268,

Fax : 1-631-389-2554

Web : www.biompa.com

MicroPhotoAcoustics Inc. follows a policy of continuous product improvement. Specifications are subject to change without notice.

MicroPhotoAcoustics Inc offers a limited warranty for its products. For full details on warranty coverage, or for further product information,

please contact MicroPhotoAcoustics Inc.