15
Combating Biofouling A design experiment by Madison Evans

Combating Biofouling

  • Upload
    norah

  • View
    47

  • Download
    1

Embed Size (px)

DESCRIPTION

Combating Biofouling . A design experiment by Madison Evans. The Issue. source: Australian Government Dept. of Agriculture. Formation of Fouled Hulls. Polymers Bacteria Algae, worms, barnacles, mussels. Michelle Hamel. Solutions. Clean Up Scrub Power Wash Biocides. Prevention - PowerPoint PPT Presentation

Citation preview

Page 1: Combating Biofouling

Combating Biofouling

A design experiment by Madison Evans

Page 2: Combating Biofouling

The Issue

source: Australian Government Dept. of Agriculture

Page 3: Combating Biofouling

1.Polymers2.Bacteria3.Algae, worms,

barnacles, mussels

Formation of Fouled Hulls

Michelle Hamel

Page 4: Combating Biofouling

Prevention● Slick silicon

coating● Copper bottom● Biocide paint

o tributyltino toxic

SolutionsClean Up● Scrub● Power Wash● Biocides

Page 5: Combating Biofouling

● invasiveo out-compete

natives for foodo sharp o damage

infrastructure● Eastern Europe →

Great Lakes o ballast water

Focus on Zebra Mussel

California Dept. of Fish and Wildlife

Page 6: Combating Biofouling

● Mussels use a group messaging system for mating and settlement

● Engineer Pelagibacter ubiqueo P. ubique to respond

to mussel serotonino express Grimontia

hollisae collagenase gene

My Solution

Page 7: Combating Biofouling

Mussel Structure

Page 8: Combating Biofouling

Cell Design

Page 9: Combating Biofouling

Serotonin Receptors

Collagenase

Quorum Sensing Molecules Serotonin Receptor Protein Synthesis

Biofilm Polymer Secretion

1 1 1

0 0 0

Muscle Serotonin Presence Collagenase Production GFP

0 0 0

1 1 1

Page 10: Combating Biofouling

● lab culturing-72 hourso nutritious

solution● dry dock

growth-48 hourso spray

Placement of Bacteria

Page 11: Combating Biofouling

Controlled tank environments● salt● freshwater● zebra mussels

o nutrients to reach reproductive age

Testing

Page 12: Combating Biofouling

● highly specific● possibility for evolution of mussels● other biofouling organisms

unaddressed● difficult to implement (growth on dry

boat)

Problems

Page 13: Combating Biofouling

● Multiple lines of bacterial spray● Universal signaling molecule of

mussels instead of just serotonin

Alternatives

Page 14: Combating Biofouling

Combating Invasive Zebra Mussels

A design experiment by Madison Evans

Page 15: Combating Biofouling

Brandelli, Adriano et al. “Production of an extracellular keratinase from Chryseobacterium sp. growing on raw feathers.” Electronic Journal of Biotechnology. 13 Jan 2005.<http://www.ejbiotechnology.info/index.php/ejbiotechnology/article/view/v8n1-6/428>

Proal, Amy. “Understanding Biofilms.” Bacterialty: Exploring Chronic Disease. 26 May 2008. <http://bacteriality.com/2008/05/26/biofilm/>

“Stopping the Mussel Invasion.” National Park Service. <http://www.nps.gov/cure/planyourvisit/mussel_facts.htm>Winner, Cherie. “Barnacles and Biofilms.” Woods Hole Oceanographic Institute. 5 Dec 2012.

<http://www.whoi.edu/oceanus/feature/barnacles-and-biofilms>Reid, D.C. et al. “Biofouling in Stirred Tank Reactors: Effect of Surface Finish.”

<http://books.google.com/booksid=g5miDB7M0WsC&pg=PA529&lpg=PA529&dq=do+biofilms+cause+drag&source=bl&ots=nGMKSiYnAV&sig=V4oho8Boac3GdoJfWf0w_dr6JZ8&hl=en&sa=X&ei=OL3VU7ezIIS3yASJqoDYDA&ved=0CDIQ6AEwAw#v=onepage&q=do%20biofilms%20cause%20drag&f=false

Teramura, Tamoko et al. “Cloning of a Novel Collagenase Gene from the Gram-Negative Bacterium Grimontia (Vibrio) hollisae1706B and

Its Efficient Expression in Brevibacillus choshinensis.” American Society for Microbiology. 22 Apr 2011. <http://jb.asm.org/content/193/12/3049.full>

Milne, Catherine T. et al. “A Comparison of Collagenase to Hydrogel Dressings in Wound Debridement.” Wounds Research. 1 Nov 2010.<http://www.woundsresearch.com/content/comparison-collagenase-hydrogel-dressings-wound-debridement>

Lucas, Jared M. et al. “A molecular, morphometric and mechanical comparison of the structural elements of byssus from Mytilus edulis and Mytilus galloprovincialis.” The Journal of Experimental Biology. 5 Apr 2012. <http://jeb.biologists.org/content/205/12/1807.full.pdf>

“Collagenase Guide” Sigma-Aldrich.<http://www.sigmaaldrich.com/life-science/metabolomics/enzyme-explorer/learning-center/collagenase-guide.html>Images Courtesy of the Australian Government Department of Agriculture and photographer Mila Zinkova.

References