Casey Anthony - FBI Chloroform Analysis (Interior)

8/4/2019 Casey Anthony - FBI Chloroform Analysis (Interior)

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Table of Contents

Case ID: 7A-TP-71176 Dr. Michael P. Rickenbach

Lab #: 081002002 TO LH

Description of Enclosures:Table of Contents (1 page)Administrative Notes (12 pages)Evidence Check-In Sheet (2 pages)Summary of Results - General (1 page)Case Notes (5 pages)Summary and HS-GC/MS (PIlEI) Data (49 pages)Copy of Peer-Reviewed Report (2 pages)Shipping (7-264) Invoice - Report + Customer Satisfaction Surveys (1 page)

Table of Contents.doc IPage: 1 of 11 12/1112008 I Initials: c; j~~: I \ \ \'


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v, 7-10-06)

Federal Bureau of InvestigationLaboratory DivisionLABORATORY WORK SHEET

To: TampaOrlando RASA Nickolas B. SavageTFO John Steven McElyea

Date: October 03,2008Case ID No,: 7A-TP-71176Lab No.: 081002002 TO LH

Reference: Communication dated October 01, 2008Your N o.:Title: CAYLEE MARIE ANTHONY MVICTIM

MISSING / ABDUCTED MINORORANGE COUNTY, FLORIDA

Date specimens received: October 02, 2008Specimens:ITEMS FROM 1998 PONTIAC SUNFIRE GT, FL TAG G63 XVQ47 Car seat (OSCO Item #30, Q-45)Q48 Steering wheel cover (OSCO Item #6, Q-41) .,Q49 Vacuum sweepings from driver side front (OSCO Item #1, Q-36)Q50 Vacuum sweepings from passenger side front (OSCO Item #2, Q-37)Q51 Vacuum sweepings from driver side rear (OSCO Item #3, Q-38)Q52 Vacuum sweepings from passenger side rear (OSCO Item #4, Q-39)Q53 Vacuum sweepings from rear dash area (OSCO Item #5, Q-40)Q54 Vacuum sweepings from spare tire wheel well (OSCO Item #7, Q-42)Q55 Debris from spare tire wheel well (OSCO Item #8, Q-43)Q56 Swab from spare tire wheel well (OSCO Item #9, Q-44)

Page 1 of 1ILab #: OS1002002 TO LHI I 12/11 /200s1 \\\$I


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(Rev. 01-31-2003)

FEDERAL BUREAU OF INVESTIGATION

Precedence: IMMEDIATE Date: 10/01/2008To: Laboratory Attn: Evidence Control CenterMichael VanarsdaleFrom: Tampa ~Contact: IA Karen,B. owa,n (ERT) ,Approved By: Hopper Ronald _Drafted By: Cowan Kar~n ,B~L . ~ , - - ~ ~Case ID #: 7A-TP-71176~ (Pending)'Title: CAYLEE MARIE ANTHONY - VICTIM

MISSING/ ABDUCTED MINORORANGE COUNTY, FLORIDA

407-838-2689

081002002

Synopsis: Set lead for laboratory examinations.Package Copy: Submitted with this EC are the items of evidenceas listed on enclosed letter dated 09/29/2008, FBI Lab Submission#6 from the Orange County Sheriff's Office, and a copy of OrangeCounty Sheriff's Office property forms H-52025 and H-60003.Enclosure: Enclosed for the FBI lab is a copy of the letterdated 09/29/2008, FBI Lab Submission #6 from the Orange CountySheriff's Office and a copy of Orange County Sheriff's Officeproperty forms H-52025 and H-60003.Details: On 07/16/2008, the Orange County Sheriff'sOffice (OCSO) opened an investigation into the mysteriousdisappearance of the above listed two-year old minor who wasallegedly last seen on 06/09/2008, by her mother, CASEY MARIEANTHONY. During their investigation, the OCSO arrested CASEYMARIE ANTHONY for obstruction, providing a false officialstatement, and child neglect.

On 09/26/2008, IA Karen B. Cowan (ERT) met with theOCSO. IA Cowan learned that the baby car seat used by CayleeMarie Anthony was in evidence storage at the OCSO. In addition,the OCSO would further process the vehicle bearing Florida tagG63 XV, on 09/27/2008. IA Cowan requested the remaining items ofevidence from the vehicle be passed to the FBI Lab for analysisin support of the investigation.

ILab #: 081 002002 TO LHI 112 /1 1120081


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To: Laboratory From: TampaRe: 7A-TP-7ll76, 10/01/2008

Property Form - H-52025Q-45 Baby car seat "Eddie Bauer"property Form - H-60003Q-36 Vacuum sweep from the driver's side frontQ-37 Vacuum sweep from the passenger side frontQ-38 Vacuum sweep from the driver's side rearQ-39 Vacuum sweep from the passenger side rearQ-40 Vacuum sweep from the rear dash areaQ-4l Steering wheel coverQ-42 Vacuum sweep from the spare tire wheel wellQ-43 Scrapings from the spare tire wheel wellQ-44 Swab from the spare tire wheel well

NooNoo- -oo

The FBI Laboratory is requested to conduct appropriateexaminations on items of evidence listed in body of Ee, bearingin mind the history of the investigation to include the possibleuse of chloroform on the victim. Please examine evidence foradditional trace evidence to include hair and fiber. If hair isfound, examine for signs of decomposition and conduct DNAanalysis if possible. Conduct any other examinations as deemedappropriate. No analysis has been conducted on any item ofevidence prior to submission to the FBI Lab.

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ILab #: 081002002 TO LHI 1 1 2 / 1 1 / 2 0 0 8 \


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To: Laboratory From: TampaRe: 7A-TP-71176, 10/01/2008

LEAD (8) :Set Lead 1: (Action)

LABORATORYAT QUANTICO, VA

NooNoo~cooThe FBI Laboratory is requested to conduct appropriateexaminations on items of evidence listed in body of EC, bearingin mind the history of the investigation to include the possibleuse of chloroform on the victim. Please examine evidence foradditional trace evidence to include hair and fiber. If hair isfound, examine for signs of decomposition and conduct DNAanalysis if possible. Conduct any other examinations as deemedappropriate. No analysis has been conducted on any item ofevidence prior to submission to the FBI Lab .

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ILab #: 081 002002 TO LHI 112111 /20081


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S h a r i f f K e v in B e a ry g l l I ] ~ j i ' 1 ; 9 1 1 = ' 1 1;ltIJ;i81Telephone: 407-254-7()()() P.o. Box 1440, Orlando, Florida 32802-1440

Visit us on the Internet: http.z/www.ccso.com

September 29, 2008FBI EvidenceControl Unit2501 Investigation ParkwayQuantico, VA 22135

FBI Lab Submission # 6

CASENUMBER:08-069208Missing Person(Child)Victim: Anthony, Caylee MarieW/F D.O.B. 8/ 9 /05Suspect: Anthony, Casey MarieW/F D.O.B.3/19/86Vehicle Description1998/Pontiac/Sunfire GTWhite/Two doors./FI tag# G63XV /VIN#:lG2JB1243W7596648

1- Synopsis:On September 26, 2008 at approximately 1400 hours I was informed by ASL MichaelVincent that he had a meeting in the Orange County Sheriff's Office with KarenCowan from the FBI: Orlando Resident Agency. According to ASL Vincent the FBIrequested more additional evidence from the vehicle bearing FI tag G63 XV VIN #1G2JB1243W7596648 . The following pieceS of evidence was collected:

II- Evidence submitted here with:Art #1ealed yellow envelope containing one plastic container of vacuum sweep.Q-36(Item # H-60003)

ILab #: OS 1002002 TO LH I \I]!11/20QSj

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Art # 2 Sealed yellow envelope containing one plastic container of vacuum sweep.(Item # H-60003) Q-37Art # 3 Sealed yellow envelope containing one plastic container of vacuum sweep.(Item # H-60003) Q-38Art # 4 Sealed yellow envelope containing one plastic container of vacuum sweep.(Item # H-60003) Q-39Art # 5 Sealed yellow envelope containing one plastic container of vacuum sweep.(Item # H-60003) Q-40

NooNoo"


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Our Agenc y apprec iates yo ur c o operatio n in this c ase.S in ce re ly y ou rs ,

O range C ounty F rensiCS1-II Ge ra rdo B la ise25 00 W . C o lo nial D riveO rla nd o , F L 3 28 044 07 -2 54 -7 12 2 e xt. 7 06 85Gerardo.blo ise@ocf l .net

#: 081 002002 TO LHI 1121111200813 \ \\~\

1 Initials: ( 1 1 1 1 ~ 1
mailto:[email protected]:[email protected]


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ECS Search SlipViolation(s):Kidnapping

Violation date:Violation Location:

Victim:ANTHONY, CAYLEE MARIE

Cross Reference:

Remarks:

Lab No: 081002002City: Tampa, FloridaAgency: FBIForm: Electronic Communication, 1 0 1 0 1 / 2 0 0 8CaselD: 7A-TP-71176 Serial: 289Latent Number:Contributor No: 7A-TP-71176Subject:

ECCDelivered to Unit: ~ _

Previous Submission(s)080911006 TO ABA HM NR HL MQTampa, FloridaFBIElectronic Comm.:09/10/2008 Serial:183Qs:46-46.2 Ks: NE Items:080811006 TO LF NR LHTampa, FloridaFBIElectronic Comm.:08/08/2008 Serial:20Qs:44-45 Ks: NE Items:080811005 TO LF NRTampa, FloridaFBIElectronic Comm.:08/08/2008 Serial:19Qs:33-43 Ks: NE Items:080805005 TO LF HM NR LHTampa, FloridaFBIElectronic Comm.:08/04/2008 Serial:6Qs:22-32 Ks:3 NE Items:080730003 TO LF MQ NRTampa, FloridaFBIElectronic Comm.:07/28/2008 Serial:3Qs:1-21 Ks:1-2 NE Items:1-2

Parcel Method and No: Federal Express - 735722460471Boxes -1 Date Received: 1 0 1 0 2 / 2 0 08 -MoreReceived in ECC: 1 0 / 0 2 / 2 0 081 0 / 0 2 / 2 0 08 12:46:11 PM - anmcgraw

ILab #: 081002002 TO LHI 112/11120081


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, 7-245 (6-19-00)

FBI LaboratoryCase Activity & Communication LogLaboratory No.: 081002002 TO LH

Date11 / 25 / 2008BY:MPi~

12103 / 2 0 08By:MPR76:~".

i \lz. \ lkJo1By:MPR

JL \Lq \ r Z l i / &By:MP~

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Case ID No.: 7A-TP-71176

Activity/Communication and OutcomeRec'd notification from Rebecca Clark that evidence was placed into theCU Evidence Room.

Called Karen Cowan (407-838-2689) and left her a voicemail with myname, contact number, and other information. Spoke with SA NickSavage (407-557-4277) and notified him that I had received the evidence.

Page 1of __FBI/DOJ

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FBI/DOJ

7-245 (6-19-00) Continuation Page

L aboratory N o.: 081002002 T O L H C ase ID N o.: 7A -T P-71176

Date Activity/Communication and Outcome/ /By: _

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Chemistry UnitEvidence Check-In Sheet

Check-In Date: December 02,2008 Case ID #: 7A -TP -71176 Examiner: RICKENBACHMode Received: Evidence Initially Received In:0 USPS 0 FedEx [2] Delivered By: [2] Boxes(# 2 ) 0 Paper Bags (# )

ECU to CU Tempor ar y E yl de nc e S to rag e

Tracking #: 0 Plastic Bags (# ) 0 Envelopes (# )Condition of External Container(s): 0 Cans (# )[2] Proper Seal(s)/Label(s) 0 Other: (Comment Below) 0 Other:GENERAL DESCRIPTION OF EVIDENCE AND CONTAINERS:Retrieved two (2) boxes that were found sealed with yellow tape.Box #1: One (1) brown paper-wrapped box (r-..;61cmx ,,-,63cmxr-v 121cm) found labeled as 'Q47.' Within this box was one (1) car seat(Q47).Box #2: One (1) brown paper-wrapped box ("-'31cm x ,,-,46cmx"-'21cm) found labeled as 'Q48.' Within this box was one (1) brownpaper bag that was found sealed with yellow tape. Within the bag wasone (1) black sterring-wheel cover (Q48). The steering wheel coverwas rubber-like with two (2) fabric-like portions on opposite sides.

Date: December 02, 2008Lab #: 081002002 TO LH

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Chemistry UnitEvidence Check-In Sheet

Specimen # Contributor # Description:

047 Art #30 ; 0-45 One (1) car seatItem # H-52025048 Art #6 . 0-41 One (1) steering wheel coverItem # H-60003

..

Date: December 02, 2008Lab #: 081002002 TO LH


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Summary of ResultsILab #: 081002002 TO LH I D r . Michael P. Rickenbach I

ICase ID #: 7A-TP-711761

Page: 1 of 1 12111 / 2 008


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N ote s -O l.do c

FBI LaboratoryChemistry Unit

Case Notes - Dr. Michael P. RickenbachDate: December 02, 2008 Laboratory #: 081002002 TO LH

FBI File/Case ID #: __:.7-"-,Ao_-~P,,----,-7.=c_1'--'17'--"6'---- _

Observations and sampling: See Check-In sheet for more info on packaging.Specimen 047 (car seat) was removed from the cardboard box and no obvious putrefactive odors werenoted. Some visible stains were observed under ambient light (fabric on right-inside [leg area], fabric onright harness strap, and fabric on left-outside [leg area] of car seat). Used an alternate light source (ALS)with orange goggles at a setting of 'CSS' to view for the presence of more stains. Took cuttings from thestained areas, placed them into test tubes, and capped.Specimen 048 (steering wheel cover) seemed to be made of a rubber-like substance with fabric-like materialon opposite ends. No obvious putrefactive odors were noted. No obvious stains were observed underambient light. Used an alternate light source (ALS) with orange goggles at a setting of 'CSS' to view for thepresence of stains on specimen 048, but none were found. Took representative cuttings from both therubber-like portion and the fabric-like portion of Q48, placed them into test tubes, and capped.

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Notes-Ol.doc

Date: December 02 2008 Laboratory #: 081002002 TO LH

Pictures:

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Notes-Ol.doc


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Notes-02.doc

FBI LaboratoryChemistry Unit

Case Notes - Dr. Michael P. RickenbachDate: December 09, 2008 Laboratory #: 081002002 TO LH

FBI File/Case ID #: _!7-,,-A~--,,-P~-_!.,7-,,-117,-,,6~ _

HS-GC-MS(PllEl): Individually placed the cuttings (that had been taken previously- see Notes-Ol.doc)for specimens 047 and 048 into two (2) 20mL headspace vials. Added 1mL of water (Millipore, 18MO 'cm)into an empty test tube (Neg. Ctrl.) and vortex-mixed. Transferred this [Neg. Ctrl.] water into a headspacevial and capped. Added 1mL of water (Millipore, 18MO'cm) into the 047 and 048 headspace vials andcapped. Prepared a 0.01% (v/v) CHCh Positive Control by adding lOUL ofCHCh (Fisher; lot: 011403) to a100mL volumetric flask and diluted to volume with water (Millipore, 18MOcm). Added 1mL of the 0.01%(v/v) CHCh Pos. Ctrl. solution into a headspace vial and capped. Placed 1mL of the Pos. Ctrl. Testmixsolution (0.01 %(v/v) of MeOH, EtOH, Acetone, Isopropanol ; lot: MPR09292008 ) into a headspace vial andcapped. Individually vortex-mixed the headspace vials for ~ 1 minute and analyzed them using the HS-GC/MS instrument while in PI/EI mode. See data printouts for parameters.

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FBILabora to ry 2501 Investigation ParkwayQuantico, Virginia 22135REPORT OF EXAMINATION

To: Tampa "rlando RASA Nickolas B. SavageTFO John Steven McElyea

Date: December 11, 2008CaseIDNo.: 7A-TP-71176 - 3&'5Lab No.: 081002002 TO LH

Reference: Communication dated October 01, 2008Your No.:Title: CAYLEE MARIE ANTHONY - VICTIM

MISSING / ABDUCTED MINORORANGE COUNTY, FLORIDA

Date specimens received: October 02, 2008The items listed below were received in the Chemistry Unit:Q47 Carseat (OSCO Item #30, Q-45)Q48 Steering wheel cover (OSCO Item #6, Q-41)This report contains the results of the chemical examinations.

Results of Examinations:Chloroform was not identified within either specimen Q47 or Q48.The following techniques were used in the analysis of the specimens: visual

inspection, alternate light source visualization, and headspace-gas chromatography/massspectrometry .

Page 1 of2For Official Use Only

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Remarks:The final disposition of the evidence will be the subject ofa separate communication.For questions regarding the content of this report, please contact Examiner Michael

Rickenbach at 703-632-7431.For questions regarding the status of the remaining forensic examinations, please

contact Request Coordinator Erin Martin at 703-632-7708.

Michael P. Rickenbach, Ph.D.Chemistry Unit

Technically reviewed and any idDate:Date:

This report contains the opinions/interpretations of the examiner(s) who issued the report.Page 2 of2081002002 TO LH

For Official Use Only


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7264 (7-7-06)FBI Laboratory Shipping InvoiceFederal Bureau ofinvestigation2501 Investigation ParkwayQuantico, VA 22135

To:

Mail Room Stamps12383

Date: 1 2 / 1 2 / 2 0 08

SA Nickolas Savage850 Trafalgar CourtSuite 400Maitland,FL 32751-7163

Telephone Num ber: _(~~), __ 8 3 _ 8 _ - _ 2 _ 6 - - , O ~ 4 _ . , - - , - . . . . . , - _(Recipient)

Carrier: o Unclassi fied-N ot Sensitiveo Unclassified-SensitiveConfidentialSecret

Classification Level:I{] FedExD USPS RegisteredD Other _Special Services:o Saturday Delivery D Return Receipt (USPS only) D Fragile Material * D Perishable Material*D Hazardous Material*? (Packaged by: _ _ Date: ,

(Signature)*asterisked items must be described belowDescript ion of Contents:One (1) Laboratory Report (1 page).One (1) Customer Satisfaction Survey (081002002 TO LH) (1 page).

Declared Value $ _(Required for International Shipments)FBI File #:

MPR

Lab #(s):7A-TP-71176 081002002 TO LH

Tracking Information: Point-of-Contact Information:Re f: L D1 23 83Dep: CU Name: Michael Rickenbachate: 12/12/2008Wgt: 0,1 L8S

DV :Ext. 7431HIPPING:SPECI~L:HANDLING:

0.00 TOT~L:

4.990.350,005.34 Unit; Chemistry Unit Rm: 4220

Svcs: PRIORITY OVERNIGHT DSRTReK' 9859 3374 2278 I T,

Documents

Casey Anthony - FBI Chloroform Analysis (Interior)