Butanol Producing

E. Coli and Algae biofuels

Austin Pruitt

The Plan

Chlorella Vulgaris

Bump Array

Sorts Particles by size

Aids in evolutionary acceleration

E. Coli

Death Galaxy

The Death Galaxy is amicrochip device withinterconnected wellsand gradient

Mimics heterogeneousmicroenvironments

Can result in rapid evolution

Evolutionary Dynamic Principles Stress gradient and microenvironments

should yield rapid evolution

Low stress allows reproduction

Mutated cells climb the gradient

Small population size in wells allows faster fixation

E. Coli Resistance to Cipro

Progress so far

Next Steps Begin UV and Bump array testing Run several death galaxies

simultaneously Attempt to evolve bacteria/algae to

thrive in different conditions

Conclusion Gained many skills through this

experience Ongoing research Better understanding of evolutionary

dynamics, and fluid mechanics

Thank you!

Sources http://www.princeton.edu/physics/puchalla/research/microfluidics/

BumpArray_1.jpg http://harmonicarts.ca/wp-content/uploads/2013/04/

chlorella_vulgaris_beij_1_0.jpg http://krisdedecker.typepad.com/photos/uncategorized/

2008/04/04/algae1.jpg http://ak2.picdn.net/shutterstock/videos/4077487/preview/stock-

footage-moscow-june-cars-stands-in-traffic-jam-on-the-city-center-june-moscow-russia.jpg

http://innovativesolarsystemsllc.com/wp-content/uploads/solar-power-generator.jpg

http://s.hswstatic.com/gif/hydropower-plant-usbr-hoover-1.jpg http://cleantechnica.com/files/2014/04/wind-turbines17.jpg http://www.prism.princeton.edu/PRISM_cleanroom/images/

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