Biology Xii project

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    Biology project

    TO ESTIMATERELATIVE

    AMOUNT OF

    CAFFEINE INVARIOUS

    BRANDS OF TEA

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    CERTIFICATE

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    1. Aim

    2. Requirement

    3. Introduction

    4. Classifcation o tea plant5. Importance o tea

    6. Extraction o ater solu!lecomponent rom tea sample"I#

    $. Remo%al o tannic acid rom fltrate"II#

    &. Extraction o ca'eine rom tannicacid ree fltrate"III#

    (. Calculation

    1). *!ser%ation ta!le

    11. Experimental results12. +recautions and expected errors

    13. !i!lio,rap-

    CONTENT

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    Extraction anddetermination o

    ca'eine content in%arious tea samples

    AIM

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    /ea0er Conical as0 unnel eatin, arran,ement amples o tea Calcium car!onate

    ilute sulp-uric acid c-loroorm

    REQUIREMENT

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    ost popular drin0s in Indiaare Tea a! Co"ee#As is t-epopularit o t-ese drin0s so is

    t-e contri!ution o India in t-eirproduction. India -as !een t-eleadin, producer o tea.

    8ea ,ros indeed9 ertile : ell

    drained soil. It require arm andmoist climate t-rou,-out t-eear. requent s-oers9 elldistri!uted o%er t-e ear

    ensures continuous ,rot- otender lea%es.

    INTRODUCTION

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    In India it ,ros mainl in lo-ills o Assam9 ills o ar;eelin,and est

    /en,al and t-e -ill o =il,irietc. 8ea also causes man-armul disease suc- asia!etes9 pro!lems in ner%ous

    sstem9 decrease in A=secretion -ic- causes increasein ine secretion and it -as=icotine -ic- is a timulant.

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    ?in,dom @ +lantae

    Class @ icotledonae

    *rder @ 8-eales

    amil 8-eaceae

    Benus @ 8-ea

    pecies @ inensis

    Cla$$i%catio o& tea

    plat

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    It is used as stimulant It -as anti%iral and ,ermicidal

    acti%it /lac0 tea is capa!le o reducin,

    c-olesterol and tri,lcerides8ea is diuretic su!stances8ea drin0in, acilitates muscle

    contraction 9 induced ! ner%estimulation.

    IM'ORTANCE OF

    TEA

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    se di'erence in t-e solu!ilito ca'eine and tannic acid inater and c-loroorm to

    separate t-em rom tea lea%es.Ca'eine is muc- more solu!lein c-loroorm t-an in ater. /uttannic acid is extensi%el

    solu!le in ater as ell asc-loroorm.

    t(eory

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    8a0e a!out 1))ml o distilledater in 25)ml conical as0.

    Add 5,m o tea and !oil it or 4

    to 5 minute. 8-en cool it andflter.

    Repeat t-is process a,ain it-1))ml o distilled ater and

    residual tea lea%e.8-e fltrate in eac- case s-ould

    !e collected. =o t-e et tea samples ma

    !e dried in an o%er andei,-ted.

    E)tractio o& *ater Sol+,leCo-poet$ &ro- Tea Sa-ple .

    Step.I

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    8-e fltrate o!tained in t-e frst step isno !oiled it- 2@3 ,m. calciumcar!onate to ,et tannic acid9 present

    in tea extract9 precipitates. *n lea%in,it or ten minutes ppt. is o!tained. ilter t-e solution it-out distur!in,

    t-e ppt careull. 8-e ppt s-ould !etranserred to flter paper onl ater all

    t-e fltrate is remo%ed.8annic acid -as !een extracted in t-e

    orm o calcium ternate -ic- ontreatment it- -droc-loric acid ,i%etannic acid.

    8-e fltrate is no ree rom tannicacid and can !e used to extractca'eine.

    Re-o0al o& Taic Aci! &ro- Filtrate .Step.II

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    8a0e tannic acid fltrate o!tained instep 2"ii# and add 1)) ml oc-loroorm in it in a separatin, unnel

    and allo it to stand or 1) minutes. A,ain t-e same procedure as done in"i#

    ix it it- t-e pre%ious one. rom t-is c-loroorm extract9

    c-loroorm ma !e distilled ore%aporated.

    8-e pale ,reen residue o!tained iscrude ca'eine.

    Repeat t-is experiment it- ot-er!rands o tea and record ouro!ser%ation in a ta!ular orm.

    E)tractio o& Ca"eie &ro- TaicAci! Free Filtrate . Step.III

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    Calc+latio$ o& 1ag( ,a2ri

    >1D 6 ,m

    >2D ).$ ,m

    +ercenta,e o ca'eine D ).$ 6 x 1))

    D 11.6F

    Calc+latio$ o& Lipto yello1 la,el

    >1D 6 ,m

    >2D ).& ,m +ercenta,e o ca'eine D 13.3F

    Calc+latio$*eig(t o& tea +$e! 3 *4g-5

    *eig(t o& Ca"eie 3 *6g-5Calc+latio 7

    'ercetage o& $ol+te ca"eie 3 *4 8 *6 ) 499

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    Calc+latio$ o& Taj Ma(al

    >1D 6 ,m

    >2D ).$ ,m

    +ercenta,e o ca'eine D 11.6F

    Calc+latio$ o& Re! La,el

    >1D 6 ,m

    >2D ).$2 ,m

    +ercenta,e o ca'eine D 12

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    Sr:o5

    Bra!a-e

    *4 *6 ;age o&ca"eie

    1 >a,-!a0ri

    6,m ).$,m 11.6

    2 Giptonellola!el

    6,m ).&,m 13.3

    3 8a; ma-al 6,m ).$,m 11.6

    4 Red la!el 6,m ).$2,m 12.)

    O,$er0atio ta,le

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    8-e tea sample o Gipton ellola!el contain -i,-est amount oca'eine and -ence H@

    Bi%es !etter taste t-an ot-ers. Is more -armul or our -ealt-.

    E)peri-et Re$+lt$

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    C-loroorm s-ould not !e smelt directlor a lon, time as it itsel is toxic.

    8-e measurement o ei,-t o tealea%es and all extract s-ould !e precise.

    8-e measurement o ei,-t o teasample and all extract s-ould !eprecise.

    8-e ater used s-ould !e distilled. Apparatus s-ould !e clean and dr. =o ater s-ould !e added to an

    extract in !eteen t-e experiment as itma ,et errors in calculation.

    =o prolon,ed -eatin, s-ould !e done asater ma e%aporate in lar,e quantitalon, it- same %olatile solutes

    'reca+tio$ a! E)pecte!Error$ 7

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    BIBLIO