Biologia plegable

SQUISHY SCIENCE: EXTRACT DNA FROM SMASHED STRAWBERRIES

ESTEFANIA JARAMILLO SALAZAR

3rd SEMESTERMEDICINE STUDENT

U.P. BFEBRUARY 11/2013

FOLDING

ESTEFANIA JARAMILLO SALAZAR

SQUISHY SCIENCE: EXTRACT DNA FROM

SMASHED STRAWBERRIES

MOLECULAR BIOLOGY

MEDICINE STUDENT3rd SEMESTER

FEBRUARY 11/2013

TEACHERLINA MARÍA MARTINEZ

SANCHEZ

BIBLIOGRAPHY

MEDICAL UTILITY.

Whether you’re a human, rat, tomato or bacterium, each of your cells will have

DNA inside of it (with some rare exceptions, such as mature red blood

cells in humans). Each cell has an entire copy of the same set of instructions, and this set is called the genome. Scientists study DNA for many reasons: They can figure out how the instructions stored in DNA help your body to function properly.

They can use DNA to make new medicines or genetically modify crops to be resistant to insects. They can solve who is a suspect of a crime, and can even use ancient DNA to reconstruct

evolutionary histories!To get the DNA from a cell, scientists

typically rely on one of many DNA extraction kits available from

biotechnology companies. During a DNA extraction, a detergent will cause the cell to pop open, or lyse, so that the

DNA is released into solution. Then alcohol added to the solution causes the

DNA to precipitate out. In this activity, strawberries will be used because each strawberry cell has eight copies of the genome, giving them a lot of DNA per cell. (Most organisms only have one

genome copy per cell.)

- Scientific American, a Division of Nature America, Inc.” Squishy Science: Extract DNA from Smashed Strawberries.” Scientific American, January 31, 2013. visited on the internet portal:http://www.scientificamerican.com/article.cfm?id=squishy-science-extract-dna-from-smashed-strawberries - Agency for Science, Technology and Research (A*STAR), Singapore. "Nucleus structure crucial to understanding diseases discovered.“ Sc ie nc e Da ily , 7 Feb. 2013. Web. visited on the internet portal: http://www.sciencedaily.com/releases/2013/02/130208110255.htm

http://www.scientificamerican.com/article.cfm?id=squishy-science-extract-dna-from-smashed-strawberries

FOLDING

This news is of vital importance, because it allow us to understand diseases and their relation with age, besides that it gives explanation of various syndromes making possible to give better treatment and prevention of disease.

INTRODUCTION

STUDENT OBSERVATION.

Have you ever wondered how scientists extract DNA from an organism? All living organisms have DNA, which is short for deoxyribonucleic acid; it is basically the blueprint for

everything that happens inside an organism’s cells. Overall,

DNA tells an organism how to develop and function, and is so

important that this complex compound is found in virtually every one of its cells. In this activity you’ll make your own

DNA extraction kit from household chemicals and use it

to separate DNA from strawberries.


I think that this is a very interesting story, curious and funny, which is available to everyone. is a

simple and effective method that provides a start to the DNA testing allowing a better understanding about the functioning of the same, opening the

mind to its utility and who knows probably reaching to some discoveries that allow us to develop new

theories in order to apply them on life.



Materials : Rubbing alcohol, Measuring cup, Measuring spoons, Salt, water, Dishwashing liquid,

Glass or small bowl, Cheesecloth, Funnel, Tall drinking glass, Three strawberries, Resealable

plastic sandwich bag, Small glass, Bamboo skewer, available at most grocery stores.

Procedure : Prepare the extraction liquid. - Put the strawberries into a resealable plastic bag - smash the strawberries. - Add three tablespoons of the

extraction liquid to the strawberries. - Squeeze the strawberry mixture - Pour the strawberry mixture

from the bag into the funnel. Let it drip through the cheesecloth and into the tall glass until there is very

little liquid left in the funnel - Pour the filtered strawberry liquid from the tall glass into the small glass. - Measure out one half cup of cold rubbing

alcohol. - Tilt the jar and very slowly pour the alcohol down its side. Pour until the alcohol has

formed approximately a one-inch-deep layer on top of the strawberry liquid. Do not let the strawberry

liquid and alcohol mix.The strawberry DNA will appear as gooey

clear/white stringy stuff.

NUCLEUS STRUCTURE CRUCIAL TO

UNDERSTANDING DISEASES DISCOVERED

Scientists from Singapore and Germany have identified that the proteins lamin A (Lmna) and lamin B receptor (Lbr) are essential for holding silent genes in their correct position at the edge of the nucleus, in the form of heterochromatin. A deviation from their normal position will cause the genes to malfunction, leading to heart failure, vascular disease and muscle wasting.For hundreds of years before this discovery, scientists were puzzled by why heterochromatin clustered at the edge of the nucleus and how it was relevant to normal cell function. This recent discovery will enable scientists to gain a better understanding of the diseases of the heart and muscles, and find cures for them in the future.The findings by Audrey Wang and Colin Stewart of A*STAR's Institute of Medical Biology and Irina Solovei, Boris Joffe and Heinrich Leonhardt of the Ludwig Maximillian University in Munich, Germany, were recently published in the prestigious journalCell.

INTRODUCTIONINTRODUCTION

Have you ever wondered how scientists extract DNA from an organism? All living organisms have DNA, which is short for deoxyribonucleic acid; it is basically the blueprint for

everything that happens inside an organism’s cells. Overall,

DNA tells an organism how to develop and function, and is so

important that this complex compound is found in virtually every one of its cells. In this activity you’ll make your own

DNA extraction kit from household chemicals and use it

to separate DNA from strawberries.

This discovery is an experimental

activity that works closely

with DNA. It helps its understanding,

leaving the knowledge for

everyone.

DNA is important to all the things that happens in the body's cells, this tells me how they are develop

and how they work.

SQUISHY SCIENCE: EXTRACT DNA FROM SMASHED

STRAWBERRIES


I think that this is a very interesting story, curious and funny, which is available to everyone. is a

simple and effective method that provides a start to the DNA testing allowing a better understanding about the functioning of the same, opening the

mind to its utility and who knows probably reaching to some discoveries that allow us to develop new

theories in order to apply them on life.



Materials : Rubbing alcohol, Measuring cup, Measuring spoons, Salt, water, Dishwashing liquid,

Glass or small bowl, Cheesecloth, Funnel, Tall drinking glass, Three strawberries, Resealable

plastic sandwich bag, Small glass, Bamboo skewer, available at most grocery stores.

Procedure : Prepare the extraction liquid. - Put the strawberries into a resealable plastic bag - smash the strawberries. - Add three tablespoons of the

extraction liquid to the strawberries. - Squeeze the strawberry mixture - Pour the strawberry mixture

from the bag into the funnel. Let it drip through the cheesecloth and into the tall glass until there is very

little liquid left in the funnel - Pour the filtered strawberry liquid from the tall glass into the small glass. - Measure out one half cup of cold rubbing

alcohol. - Tilt the jar and very slowly pour the alcohol down its side. Pour until the alcohol has

formed approximately a one-inch-deep layer on top of the strawberry liquid. Do not let the strawberry

liquid and alcohol mix.The strawberry DNA will appear as gooey

clear/white stringy stuff.


STRAWBERRIESPreparation of the extraction liquid- Chill the rubbing alcohol in the freezer. (You’ll need it later.)- Mix one half teaspoon of salt, one third cup of water and one tablespoon of dishwashing liquid in a glass or small bowl. Set the mixture aside. This is your extraction liquid. Why d o y o u think the re is d e te rg e nt in the e x tra c tio n liq uid?- Completely line the funnel with cheesecloth. Insert the funnel tube into the tall drinking glass (not the glass with the extraction liquid in it).- Remove and discard the green tops from the strawberries.


STRAWBERRIES

Extra: the experiment can be make it in several ways:- Grab some oatmeal or kiwis from the kitchen and try it again! Which fo o ds g ive y o u the m o s t DNA?- Try to tweak different variables in this activity to see how you could change your strawberry DNA yield. For example, you could try starting with different amounts of strawberries, using different detergents or different DNA sources (such as oatmeal or kiwis). Which c o nd itio ns g ive y o u the be s t DNA y ie ld?


STRAWBERRIESMaterials

Rubbing alcohol, Measuring cup, Measuring, spoons, Salt, water, Dishwashing liquid (for hand-washing dishes), Glass or small bowl, Cheesecloth, Funnel, Tall drinking glass, Three strawberries, Resealable plastic sandwich bag, Small glass jar, (such as a spice or baby food jar), Bamboo skewer, available at most grocery stores. (If you use a baby food or short spice jar, you could substitute a toothpick for the skewer.)

OBSERVATION

I think that this is a very interesting story, curious and funny, which is available to

everyone. is a simple and effective method that provides a start to the DNA testing allowing a better understanding

about the functioning of the same, opening the mind to its utility and who

knows probably reaching to some discoveries that allow us to develop new theories in order to apply them on life.

NUCLEUS STRUCTURE CRUCIAL TO UNDERSTANDING DISEASES DISCOVERED

This news is of vital importance, because it allow us to understand diseases and their relation with age, besides that it gives explanation of various syndromes making possible to give better treatment and prevention of disease.


NUCLEUS STRUCTURE CRUCIAL TO

UNDERSTANDING DISEASES DISCOVERED

Scientists from Singapore and Germany have identified that the proteins lamin A (Lmna) and lamin B receptor (Lbr) are essential for holding silent genes in their correct position at the edge of the nucleus, in the form of heterochromatin. A deviation from their normal position will cause the genes to malfunction, leading to heart failure, vascular disease and muscle wasting.For hundreds of years before this discovery, scientists were puzzled by why heterochromatin clustered at the edge of the nucleus and how it was relevant to normal cell function. This recent discovery will enable scientists to gain a better understanding of the diseases of the heart and muscles, and find cures for them in the future.The findings by Audrey Wang and Colin Stewart of A*STAR's Institute of Medical Biology and Irina Solovei, Boris Joffe and Heinrich Leonhardt of the Ludwig Maximillian University in Munich, Germany, were recently published in the prestigious journalCell.


The nucleus has the information as of chromatin

necessary for the cell to grow, develop and reproduce.

scientists showed that AYB like sheet proteins are

important for the organization of chromatin in the nucleus. if there is a lack of these will be

a collapse in the nuclear center causing a disruption of

gene expression and development of affected

skeletal muscle, resulting in muscle failure.


the findings on changes in the

position of chromatin can

contribute to the exploration of heart failure.


This news takes researchers a step closer to understanding

the nucleus and the development of new

treatments for common diseases. It could be

particularly relevant for Mexico, since, like other developed countries, are

facing the population aging, heart failure, vascular disease

and muscle atrophy that increase with age.


Two major advances have been made in the last

years, the experimentation with the cloning and the discovery

of the Human Genome Mapping considered the

greatest history feat

OBSERVATION

This news is of vital importance, because it allow us to understand

diseases and their relation with age, besides that it gives

explanation of various syndromes making possible to give better treatment and prevention of

disease.

MEDICAL UTILITY

MEDICAL UTILITY.

Whether you’re a human, rat, tomato or bacterium, each of your cells will have

DNA inside of it (with some rare exceptions, such as mature red blood

cells in humans). Each cell has an entire copy of the same set of instructions, and this set is called the genome. Scientists study DNA for many reasons: They can figure out how the instructions stored in DNA help your body to function properly.

They can use DNA to make new medicines or genetically modify crops to be resistant to insects. They can solve who is a suspect of a crime, and can even use ancient DNA to reconstruct

evolutionary histories!To get the DNA from a cell, scientists

typically rely on one of many DNA extraction kits available from

biotechnology companies. During a DNA extraction, a detergent will cause the cell to pop open, or lyse, so that the

DNA is released into solution. Then alcohol added to the solution causes the

DNA to precipitate out. In this activity, strawberries will be used because each strawberry cell has eight copies of the genome, giving them a lot of DNA per cell. (Most organisms only have one

genome copy per cell.)

MEDICAL UTILITY

Allow us to have a more empirical

knowledge about DNA and its importance

MEDICAL UTILITY

Allow us to have a more conscious

understanding of the importance of the

correct position of the genes in the nucleus and which may cause abnormalities in the

same, like a malfunction of the

heart's blood vessels and muscles

BIBLIOGRAPHY

- Scientific American, a Division of Nature America, Inc.” Squishy Science: Extract DNA from Smashed Strawberries.” Scientific American, January 31, 2013. visited on the internet portal:http://www.scientificamerican.com/article.cfm?id=squishy-science-extract-dna-from-smashed-strawberries

- Agency for Science, Technology and Research (A*STAR), Singapore. "Nucleus structure crucial to understanding diseases discovered.“ Sc ie nc e Da ily , 7 Feb. 2013. Web. visited on the internet portal: http://www.sciencedaily.com/releases/2013/02/130208110255.htm

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Biologia plegable