Biochem 6th Sem Sect 4

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    -VE TEST forVitamin A

    - Colourless- Clear fluid

    -So, I guess

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    Based on the fact that

    concentrated sulphuric acid

    Takes up H20 from retinoland yields colored product

    (blue)

    Since it is a fat soluble vitamin,we shall dissolve it with

    chloroform.

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    Add 2 drops of fish oil into a test tube

    Add 1-2 ml of chloroformLastly, add 1-2 drops of concentrated sulphuric acid,and blue coloration will be form.

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    Vitamin A

    Vitamin A exist in 3 biological active forms ;

    Retinol

    Retinal Retinoic acid

    Vitamin A is derived from the provitamin

    carotenes by carotenase enzymes which occur

    in the interstinal mucosa and liver.

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    Vitamin A

    Carotenes = dark green yellow vegetables and

    fruits ; carrots, sweet potatoes ,spinach,

    apricots and green leafy vegetables

    Performed vitamin A from animal sources ;

    liver, egg yolk, butter, whole milk and fish liver

    oil

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    Vitamin A

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    Uric Acid

    It is the result of purine catabolism.

    Principle :

    Uric acid + O2 + 2 H2O ------> Allantoine +

    H2O2 + CO2

    2 H2O2 + DCPS + 4- Amino phenazone ------>Red Quinonimine + 4 H2O

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    Uric Acid

    Procedure :

    1. Prepare two test tube labelled sample and standard

    2. Add 1.0ml of the reagent into both tubes

    3. Add 50 ul of standard (uric acid) in test tube labelledstandard and 50 ul of sample in test tube labelledsample.

    4. Shake both test tube and leave it for 10 minutes inroom temperature.

    5. Calculate the absorbance of sample and standard.

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    Dont Forget to do

    several wash

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    Calculate theabsorbance

    of sampleand standard

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    Jangan Lupa bawak kalkulator(susah nak pinjam2 member)

    - Pak kura

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    Mutant forms of PRPP

    synthetase

    Partial HGPRtase deficiency

    Abnormalities

    in purine catabolism

    Abnormalities in other

    metabolic pathway

    Deficiency in G-6-

    phosphatase ( Von

    Gerk's disease)

    Elevation of

    glutathione

    reductase activity

    Increase

    consumption of

    meat

    Malignancy and

    chemotherapy

    Kidney diseases,

    decrease uric acid

    excretion

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    DecreaseUric Acid

    Production

    Inhibit XanthineOxidase

    Anti-inflammatory

    UricosuricDrugs

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    Maka, apabila kamu telah selesai (dari sauatu urusan),kerjakanlahdengan sungguh-sungguh (urusan) yang lain. Hanya kepadaTuhanmu hendaknya kamu berharap.

    ( Alam Nasyrah

    ayat 7-8 )

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