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8/6/2019 Biochem 6th Sem Sect 4
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-VE TEST forVitamin A
- Colourless- Clear fluid
-So, I guess
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Based on the fact that
concentrated sulphuric acid
Takes up H20 from retinoland yields colored product
(blue)
Since it is a fat soluble vitamin,we shall dissolve it with
chloroform.
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Add 2 drops of fish oil into a test tube
Add 1-2 ml of chloroformLastly, add 1-2 drops of concentrated sulphuric acid,and blue coloration will be form.
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Vitamin A
Vitamin A exist in 3 biological active forms ;
Retinol
Retinal Retinoic acid
Vitamin A is derived from the provitamin
carotenes by carotenase enzymes which occur
in the interstinal mucosa and liver.
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Vitamin A
Carotenes = dark green yellow vegetables and
fruits ; carrots, sweet potatoes ,spinach,
apricots and green leafy vegetables
Performed vitamin A from animal sources ;
liver, egg yolk, butter, whole milk and fish liver
oil
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Vitamin A
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Uric Acid
It is the result of purine catabolism.
Principle :
Uric acid + O2 + 2 H2O ------> Allantoine +
H2O2 + CO2
2 H2O2 + DCPS + 4- Amino phenazone ------>Red Quinonimine + 4 H2O
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Uric Acid
Procedure :
1. Prepare two test tube labelled sample and standard
2. Add 1.0ml of the reagent into both tubes
3. Add 50 ul of standard (uric acid) in test tube labelledstandard and 50 ul of sample in test tube labelledsample.
4. Shake both test tube and leave it for 10 minutes inroom temperature.
5. Calculate the absorbance of sample and standard.
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Dont Forget to do
several wash
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Calculate theabsorbance
of sampleand standard
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Jangan Lupa bawak kalkulator(susah nak pinjam2 member)
- Pak kura
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Mutant forms of PRPP
synthetase
Partial HGPRtase deficiency
Abnormalities
in purine catabolism
Abnormalities in other
metabolic pathway
Deficiency in G-6-
phosphatase ( Von
Gerk's disease)
Elevation of
glutathione
reductase activity
Increase
consumption of
meat
Malignancy and
chemotherapy
Kidney diseases,
decrease uric acid
excretion
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DecreaseUric Acid
Production
Inhibit XanthineOxidase
Anti-inflammatory
UricosuricDrugs
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Maka, apabila kamu telah selesai (dari sauatu urusan),kerjakanlahdengan sungguh-sungguh (urusan) yang lain. Hanya kepadaTuhanmu hendaknya kamu berharap.
( Alam Nasyrah
ayat 7-8 )
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