Benthic Macroinvertebrate Sampling - Maryland ... Benthic Macroinvertebrate Sampling Certification •

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  • Benthic Macroinvertebrate Sampling Certification

    • Complete spring training

    • Pass written exam at the end of spring training (new or renewing applicants only)

    • Complete field audit (March 1 – April 30); payment due

    • Submit sample for DNR lab analysis

    • Your name and affiliation posted on MBSS Registry

    • Acquire all necessary equipment and supplies

  • Benthic Macroinvertebrate Sampling

    “…provide a representative sample of the community composition and relative abundance of benthic macroinvertebrates in favorable habitats (habitats supporting the greatest benthic diversity)”

    MBSS Sampling Manual 2017; Section 5.13.1

  • Benthic Macroinvertebrate Sampling

    • March 1 – April 30 Index Period

    • Same 75 m as summer

    • D net (500 or 540 micron)

    • 20 square feet of BEST AVAILABLE habitat (pooled into one container)

    • Sample water first

    MBSS Sampling Manual 2017, Section 5.13.1

  • Focus on: Habitats with good FLOW Habitats that are STABLE

    MBSS Sampling Manual 2017; Section 5.13.1, #2

  • Avoid

    Silt in pools Shifting sand in runs

  • Determine Sampleability

    Too deep High flow

    Can’t see habitats Dry stream

    Marsh Impoundment

    Tidal No permission

    MBSS Sampling Manual 2017; Section 5.13.1, #1

  • Equipment Checklist

    D-net and spare Sieve Bucket

    Sample buckets and labels 95% Ethanol (2 L/site) Waders (no felt soles)

    Sampling Manual Data Sheets

    Pencils First Aid Kit

    Flagging/Paint 100 m measuring tape

    MBSS Sampling Manual 2017; Appendix C

  • Lower Case D Upper Case D

  • Habitats to Sample

    • Riffles

    MBSS Sampling Manual 2017; Section 5.13.1, #3

  • Riffle

    Start at downstream edge and work upstream

    Variety of substrate types and velocities

    MBSS Sampling Manual 2017; Section 5.13.1, #3

  • MBSS Sampling Manual 2017; Section 5.13.1, #3

  • 500 or 540 micron mesh

    No holes

    MBSS Sampling Manual 2017; Section 5.13.1

  • Disturb habitat 5-8 cm below substrate surface

    MBSS Sampling Manual 2017; Section 5.13.1, #3

  • Habitats to Sample

    • Riffles • Rootwad/Woody debris

    MBSS Sampling Manual 2017; Section 5.13.1, #2

  • Rootwad

    Woody debris

    D-net downstream; rub wood with hand or brush

    MBSS Sampling Manual 2017; Section 5.13.1, #3

  • MBSS Sampling Manual 2017; Section 5.13.1, #3

  • Several jabs/rootwad Sweep through disturbed water

    MBSS Sampling Manual 2017; Section 5.13.1, #3

  • Habitats to Sample

    • Riffles

    • Leaf pack • Rootwad/Woody debris

    MBSS Sampling Manual 2017; Section 5.13.1, #2

  • Leaf pack

    MBSS Sampling Manual 2017; Section 5.13.1, #3

  • What’s 1 sq. ft.? A moderate handful

    MBSS Sampling Manual 2017; Section 5.13.1, #3

    Partially decomposed leaves preferred

  • Habitats to Sample

    • Riffles

    • Leaf pack • Rootwad/Woody debris

    • Macrophytes

    MBSS Sampling Manual 2017; Section 5.13.1, #2

  • Macrophytes MBSS Sampling Manual 2017; Section 5.13.1, #3

  • Habitats to Sample

    • Riffles

    • Leaf pack

    • Rootwad/woody debris

    • Macrophytes

    • Undercut banks

    MBSS Sampling Manual 2017; Section 5.13.1, #2

  • Undercut bank – soil and/or rock (not vegetation)

    MBSS Sampling Manual 2017; Section 5.13.1, #3

  • Habitats to Sample

    • Gravel/peat/clay/detritus/sand/silt/stable refuse (only when necessary)

    • Riffles

    • Leaf pack

    • Rootwad/woody debris

    • Macrophytes

    • Undercut banks

    MBSS Sampling Manual 2017; Section 5.13.1, # 2

  • Total must be 20 sq. ft.

    MBSS Sampling Manual 2017; Section 5.13.1, #3

    Tire, engine block, Shopping cart

  • Flow

    75 m

    Walk site to estimate habitat proportions

    MBSS Sampling Manual 2017; Section 5.13.1, #3

  • Total must be 20 sq. ft.

    MBSS Sampling Manual 2017; Section 5.13.1, #3

    1 6 3 1

  • Flow

    1

    3

    4

    2

    5 6

    7 8

    9

    18

    11

    12

    13

    15 16

    17 19

    20

    14 1/3

    1/3 1/3

    75 m

    MBSS Sampling Manual 2017; Section 5.13.1, #3

  • Upstream end of site

    75 m

    Rare case – OK to sample upstream of site

    MBSS Sampling Manual 2017; Section 5.13.1, #3

  • Partially submerge sieve bucket

    MBSS Sampling Manual 2017; Section 5.13.1, #3

  • Rinse/use forceps to clean D Net

    MBSS Sampling Manual 2017; Section 5.13.1, #3

  • Remove fish, mussels, salamanders, turtles, large sticks, stones over 3 cm, large leaves, etc. Crayfish stay IN the sample.

    MBSS Sampling Manual 2017; Section 5.13.1, #3

  • MBSS Sampling Manual 2017; Section 5.13.1, #3

  • MBSS Sampling Manual 2017; Section 5.13.1, #3

  • MBSS Sampling Manual 2017; Section 5.13.1, #3

  • MBSS Sampling Manual 2017; Section 5.13.1, #3

  • Up and Down; Not side-to-side

    MBSS Sampling Manual 2017; Section 5.13.1, #3

  • MBSS Sampling Manual 2017; Section 5.13.1, #3

    Thoroughly clean sieve bucket; Minimize water into sample bucket

  • MBSS Sampling Manual 2017; Section 5.13.1, #4

  • Sample (solids)

    Ethanol

    MBSS Sampling Manual 2017; Section 5.13.1, #4

    MBSS 1 of 2

    The total volume of preservative plus solids should be about twice the volume of solids

  • Pencil

    MBSS Sampling Manual 2017; Section 5.13.1, #4

    Lid on and gently invert and mix

  • MBSS Sampling Manual 2017; Section 5.13.1, #5 and Appendix G

  • Benthic Duplicate Analysis 214 MBSS sites had duplicate benthic field samples (2000-2016)

    The IBI ranges from 1 to 5

    96% of sites had a difference in BIBI scores ≤1.0

  • Benthic Duplicate Analysis • Mean difference in

    duplicate MBSS BIBI scores = 0.37

    • Max difference in duplicate BIBI scores = 2.25

    For MBSS certification the difference in BIBI scores between your sample and MBSS should be ≤1.0

  • Lot C

    Meet here! X

  • Lot A

    Lot C

    Meet here! X

    Benthic Macroinvertebrate Sampling Certification Benthic Macroinvertebrate Sampling Benthic Macroinvertebrate Sampling Focus on:�Habitats with good FLOW�Habitats that are STABLE Avoid� Determine Sampleability��Too deep�High flow�Can’t see habitats�Dry stream�Marsh�Impoundment�Tidal�No permission Equipment Checklist�� D-net and spare�Sieve Bucket�Sample buckets and labels �95% Ethanol (2 L/site)�Waders (no felt soles)�Sampling Manual�Data Sheets�Pencils�First Aid Kit�Flagging/Paint�100 m measuring tape� Slide Number 8 Slide Number 9 Slide Number 10 Slide Number 11 Slide Number 12 Slide Number 13 Slide Number 14 Slide Number 15 Slide Number 16 Slide Number 17 Slide Number 18 Slide Number 19 Slide Number 20 Slide Number 21 Slide Number 22 Slide Number 23 Slide Number 24 Slide Number 25 Slide Number 26 Slide Number 27 Slide Number 28 Slide Number 29 Slide Number 30 Slide Number 31 Slide Number 32 Slide Number 33 Slide Number 34 Slide Number 35 Slide Number 36 Slide Number 37 Slide Number 38 Slide Number 39 Slide Number 40 Slide Number 41 Slide Number 42 Slide Number 43 Slide Number 44 Slide Number 45 Slide Number 46 Benthic Duplicate Analysis Benthic Duplicate Analysis Slide Number 49 Slide Number 50

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