ASX-400 Operators Manual

8/10/2019 ASX-400 Operators Manual

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ASX-400 Auto SamplerOperators Manual


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Product Warranty Statement

SD Acquisition, Inc., DBA CETAC Technologies (CETAC), warrants

any CETAC unit manufactured or supplied by CETAC for a period

beginning on t he da te of shipment a nd end ing on th e sooner to occur of:

(a) the date that is twelve (12) months from the date of installation, or

(b) the date that is thirteen (13) months from the date of shipment.

Units found in the reasonable judgement of CETAC to be defective in

material or workmanship will be repaired or replaced by CETAC

with out cha rge for parts an d labor. CETAC reserves the right to

change or improve the design of any unit without assuming any

obliga tion to modify a ny unit previously ma nufa ctured.

This w ar ra nty does not cover an y un it tha t h as been subject t o misuse,

neglect, negligence, or accident. The wa rra nt y does not apply to a nydamage to the unit that is the result of improper installation or

maintenance, or to any unit that has been operated or maintained in

any way contrary to the instructions specified in the CETAC

instruction a nd opera tion manu al. Opera tion of the CE TAC un it inside

a laboratory fume hood is contra-indicated and will void the warranty.

Any attempt to repair or alter any CETAC unit by anyone other than

by CETAC a uth orized personnel or a gents will void this wa rra nty . If

any non-CETAC component is installed in the CETAC manufactured

unit w ithout the approval of CETAC, the wa rra nty w ill be voided. In

addition, this warranty does not extend to repairs made necessary by

the use of parts, accessories or fluids which are either incompatible

with the unit or adversely affect i ts operation, performance or

dura bili ty. CETACS obliga tion under this w ar ra nty is strictly an d

exclusively limited to repair or replacement of defective CETAC parts,

and no claim of breach of warranty shall be cause for cancellation or

recission of the contra ct of sale of any unit .

The foregoing express warranty is in l ieu of all other warranties,

expressed or implied, including warranties of merchantabili ty and

fitness for a par ticular purpose. CE TAC sha ll not be bound by an y

representations or statements on the part of i ts employees or agents

whether oral or in writing and including any made in catalogues and

other promotional material including technical details and

specifications except where such representations and statements are

expressly ma de pa rt of this contra ct. CE TAC a ssumes no responsibilityfor incidental, consequential or other damages, even if advised of such a

possibility, including but not limited t o loss or da ma ge of property , loss

of revenue, loss of use of the unit, loss of time, or inconvenience.

CETACs liability on any claim for loss or damage arising out of the

sale, resale or use of any of its products shall in no event exceed the

selling price of the un it.


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Purchaser shall indemnify CETAC against any claim or l iabil i ty which

may be asserted as relates to the following: (i) the use to which any

product supplied hereunder is put infringes the patent, copyright or

other intellectual property rights of a ny third part y; or (ii) an y l iabil ity

resulting from the fai lure by Purchaser to observe the terms of this

Warranty .

Returned Product Procedures

Claims for shipment damage (evident or concealed) must be filed with

th e car rier by the buyer. CE TAC must be notified w ithin ninet y (90)

day s of shipment of incorrect mat erials. No product ma y be returned,

whether in warranty or out of warranty , wi thout f i rs t obtaining

a pproval from CETAC. No replacements w ill be provided nor repairs

made for products return ed with out such approval . Any returned

product must be accompan ied by a retur n a uthorization number. Theexpense of returning the unit to CETAC for service will be paid by the

buyer. The sta tus of any product returned lat er than thirty (30) day s

after issuance of a return authorization number will be subject to

review . Shipment of repaired products wil l generally be mad e forty

eight (48) hours a fter t he receipt.

Products may not be returned which are contaminated by radioactive

materials, infectious agents, or other materials consti tuting health

ha za rds t o CETAC employees.

Returned Product Warranty DeterminationAfter CETAC S examinat ion, wa rran ty or out of wa rran ty s ta t us wi ll be

determined. If a w ar ra nted defect exists, the product wil l be repaired

at no cha rge and shipped prepaid back to the buyer. If the buyer

desires an air freight return, the product will be shipped collect.

War ra nty r epairs do not extend the original w ar ra nty period.

If an out of warranty defect exists, the buyer shall be notified of the

repair cost. At such time the buyer must issue a va lid purcha se order

to cover the cost of repair and freight, or authorize the products to be

shipped back as is , a t t he buyer s expense. Fa ilure to obtain a purchase

order number approval within fifteen (15) days of notification will

result in the products being return ed as is , at the buyers expense.


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COPYRIGHT

Copyright SD Acquisition, Inc., DBACE TAC Technologies480113 Version 1.0, Rev. 1, Mar ch, 2002

REPRODUCTION

All right s reserved. Reproduction ortransmission of this document in whole orin part , and by any means without theexpress written consent of the copyrightowner or authorized agent is prohibited.Requests for additional copies of this, orany other C ETAC publication, can be filledby contact ing an authorized distributor or

CE TAC TechnologiesCust omer Serv ice & Support14306 Industr ial Roa dOmah a, Nebra ska 68144, US AP hon e (800) 369-2822 (US A only )

P hon e (402) 733-2829Fa x (402) 733-1932E-mail [email protected]

DISCLOSURE

This document contains CETACproprietary data and is provided solely toits customers for their express benefit ofsafe, efficient operation and maintenanceof the product described herein. Use ordisclosure of CETAC proprietary data forthe purpose of manufacture orreproduction of the item described herein,or any similar item, is prohibited, anddelivery of this document shall not

constitute any license or impliedauthorizat ion to do so.

REVISIONS

CE TAC Technologies str ives to provide thescientific community with an unparalleledcombination of effective technology andcontinuing value. Modular upgra des forexisting instruments will continue to be aprime considera tion as designs progress.

CETAC Technologies reserves the right torevise t his document and /or improve

products described herein at any timewithout notice or obligat ion. Warrantyregistrat ion entit les the named ownerexclusively to man ual cha nge pages/neweditions as they a re published.

SAFETY

Instruments, accessories, components or

other associated materials may not bereturned to CETAC Technologies ifcontaminated with biohazard orradioactive materials, infectious agents, oran y other ma teria ls and /or conditions thatcould const itute a health or injury hazardto CE TAC employees. Ca ll Cust omerService and Support if there is anyquestion or doubt relative todecontaminat ion requirements.

CAUTION and WARNING statements, asapplied in this document, shall beinterpreted consistent with the followingcontext: CAUTION applies only topotential property damage conditions;WARNING applies to potential personalinjury conditions, in combination with orexclusive of potential property damage.

WARNING

The handling of organomercurialconcentrates which may be used in thepreparat ion of process standar ds presentsa substantial (potentially lethal) safetyhaza rd. Only an experienced,professionally trained organo-metallicchemist, knowledgeable and skilledspecifically in the safe handling oforganomercurials (using approvedapparatus and approved protect ionmeasures in an approved facility) should

at tempt to prepare dilutedorganomercurial process standards fromconcentrates.

NOTE

SD Acquisition, Inc., DBA CETACTechnologies assumes no liability for thehandling of organomercurial concentratesor the preparat ion, handling, or use ofdiluted organomercurial process standards.Instea d, C ETAC Technologies recommendsuse of appropriate standard referencematerials to validate sample preparat ion(dissolution/digest ion) and use of inorga nic

mercury standards for instrumentcalibration.

All user-serviceable components arespecifically identified in this document assuch; the balance shall be assumed torequire the expertise of a factory servicetechnician/engineer for a djustment, repair,


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replacement, modificat ion, etc. Other s notso qualified and performing these actionsshall do so at their own risk. Furthermore,never operate t he instrument without f irst

reading and understanding the ASX-400

Operator s Manu al and ensuring that it is

operated safely and properly.

ORIGINAL PACKAGING

Retain original factory packaging formoves and factory return shipments.Shipping in anything other than theoriginal f it ted foam and container canresult in incidental dam age from w hich thepurchaser will not be protected underwa r ra n t y .

Under all conditions the user must observe safe laboratoryprocedures during the operation of this product.

WARNING


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Operators Manual Addendum

Notices and Compliance Declarations

AD-1

FEDERAL COMMUNICATIONS

COMMISSION (FCC) NOTICE

This equipment has been tested and foundto comply with the limits for a Class Adigita l device, pursuant to Part 15 of theFCC R ules. These limits are designed toprovide reasonable protection againstharmful interference in a commercialinstalla t ion.

This equipment generates, uses, and canradiate radio frequency energy and, if notinstalled and used in accordance with theinstruct ions, may cause harmful

interference to radio communications.Operat ion of this equipment in aresidential environment is likely to causeharmful interference, in which case theuser will be required to correct theinterference at his own expense.

MODIFICATIONS

The FCC requires the user to be notifiedthat any changes or modificat ions made tothis device that are not expressly approvedby CE TAC may void the user's auth ority tooperat e th e equipment.

CABLES

Connections to this device must be madewit h shielded cables wit h meta llic RFI/EMIconnector hoods to maintain compliancewith FCC Rules and Regulat ions.

CANADIAN NOTICE

This digital apparatus does not exceed theClass A limits for radio noise emissionsfrom digita l apparatus as set out in theinterference-causing equipment standardentit led "Digita l Apparatus. " ICES-003 ofthe D epartment of Communicat ions.

AVIS CANADIEN

Cet appareil numerique respecte leslimites de bruits radioelectriquesapplicables aux appareils numeriques deClasse A prescrites dans la norme sur le

mat eriel brouilleur: "AppareilsNumeriques," NMB -003 edictee par leministre des Communications.


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AD-2

POWER CORD SET REQUIREMENTS

The power cord set supplied with yourinstrument meets the requirements of thecountry where you purchased theinstrument.

I f you use the instrument in anothercountry, you must use a power cord setthat meets the requirements of thatcountry.

This equipment is designed for connection to a grounded (earthed) outlet. Thegrounding type plug is an important safety feature. To reduce the risk ofelectrical shock or damage to the instrument, do not disable this feature.

To reduce the risk of fire hazard and electrical shock, do not expose the unit to rain orhumidity . To reduce the risk of electrical shock, do not open the cabinet. All maintena nce

is to be performed by an Authorized C ETAC Ser vice Provider.Protection provided by the equipment may be impaired if the equipment is used in amanner not specified by the manufacturer.

CLEANING INSTRUCTIONS

To clean t he exterior sur faces of the instru ment, complete the following steps:

1 Shut down and unplug the instrument.

2 Wipe the instrum ent exterior surfacesonly using a t owel dampened with alab-grade cleaning agent.

3 Repeat step 2, using a towel dampenedwith clear water.

4 Dry th e instrument exterior using a drytowel.

Do not allow any liquid to enter the instrument cabinet, or come into contact with

any electrical components. The instrument must be thoroughly dry before youreconnect power, or turn the instrument on.

COOLING FAN OBSTRUCTION

The instrum ent cooling fan (s) shall rema in unobstructed at a ll times. Do not operate theinstru ment if the cooling fan(s) ar e blocked or obstructed in a ny ma nner.

ENVIRONMENTAL

Operat ing Temperat ure: 10 to 30CRelat ive Humidit y 0%to 95%

WARNING

CAUTION

WARNING


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AD-3

AVERTISSEMENTPOUR UNE PROTECTION CONTINU

CONTRE LES RISQUES DINCENDIE,REMPLACER UNIQUEMENT PAR DESFUSIBLES DE MME TYPE ETAMPRAGE.

AVERTISSEMENTTOUT CONTACT AVEC LES HAUTESTENSIONS PEUT ENTRAINER LA MORTOU DES BLESSURES SVRES. CEPANNEAU NE DOIT TRE ENLEVE QUEPAR UN RPARATEUR QUALIFI.

AVERTISSEMENTTOUT CONTACT AVEC LES HAUTESTENSIONS PEUT ENTRAINER LA MORTOU DES BLESSURES SVRES. CE

PANNEAU NE DOIT TRE ENLEVE QUEPAR UN RPARATEUR QUALIFI.

AVERTISSEMENTTOUT CONTACT AVEC LES HAUTESTENSIONS PEUT ENTRAINER LA MORTOU DES BLESSURES SVRES. CEPANNEAU NE DOIT TRE ENLEVE QUEPAR UN RPARATEUR QUALIFI.

AVERTISSEMENTTOUT CONTACT AVEC LES HAUTESTENSIONS PEUT ENTRAINER LA MORTOU DES BLESSURES SVRES. CE


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AD-4

PANNEAU NE DOIT TRE ENLEVE QUEPAR UN RPARATEUR QUALIFI.

AVERTISSEMENTCOURANT DE FUITE LEVFORNIRUNE MISE LA TERRE EFFICACE.

.

WARNINGHIGH LEAKAGE CURRENT -

ENSURE PROPER GROUNDING


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Contents


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Contents

Preface xii

Who Sh ould Rea d This B ook xii

How to U se This B ook xii

Convent ions U sed in This B ook xiiiInstructions xiii

Menu Items xiv

Terminology xiv

Notes xv

Cautions xv

Warnings xv

Where to G o for More In forma tion xvi

1 Introduction 12

Auto Sa mpler St a nda rd Components 12

Opt iona l Accessories 16

2 Preparing for Installation 22

Choosing a Loca tion 22

Space Requirements 22

Wat er Requirements 22

P ower Requirements 23

U npa cking th e ASX-400 Aut o S a mpler 23


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ASX-400 Auto Sampler Operators Manual

Contents

vii

3 Installing the Auto Sampler 32

Mountin g th e Z-D rive Assembly 33

Attaching the Z-drive Assembly to theAuto Sa mpler Arm 34

Insta lling the Sa mple P robe 34

Assembling a nd P lacing the Sa mple Via l Ra ck 37

Est a blishing Ext erna l Connections 38

Connecting the Auto Sa mpler to the P ower Source 38

Connecting th e ASX-400 to an Ana lytical In str ument 38

Connecting th e ASX-400 Aut o Sa mplerto the Host Computer 39

Esta blishing a Serial Communicat ions Int erfa ce 310

Esta blishing a n IE EE Communica tions Int erfa ce 311

4 Verifying Installation 42

Testing the Int erface 42

Checking th e Auto Sa mpler Components 47

Testing the Sa mple P robe 47


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Contents

viii

5 Using the Auto Sampler 52

Est a blishing Optima l Opera ting Conditions 52

Creat ing the Lab Environment 52

Replacing Auto Sa mpler Components 53

P urchasing Supplies 54

St a rt ing the Auto Sa mpler 55

Shut t ing Down the Auto Sa mpler 56

Flushing the Rinse Sta t ion a nd Flow P a th 56

6 Maintaining the Auto Sampler 62

Clean ing the Auto Sa mpler 62

Da i ly External Cleaning 63

Weekly Clean ing 63

Checking for Lea ks 65

Repla cing P erista ltic P ump Tubing 66

Repla cing th e Sa mple P robe 67

Repla cing the Rinse St a tion Tubing 67

Repla cing th e Sa mple Tra y 68


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Contents

ix

7 Troubleshooting the Auto Sampler 72

P ower Syst em P roblems 72

Int erface P roblems 73

RS -232 Ca ble P roblems 73

Softwa re Configuration P roblems 73

Z-D rive Assemb ly P roblems 7-4

Glossary G2


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Preface


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Preface

The ASX -400 Au to Sampl er Operat ors Manual expla ins th e proceduresfor insta lling, using, an d maint a ining the CETAC ASX-400. It also

provides information about troubleshooting ASX-400 problems anddescribes th e design of the Auto Sam pler.

Who Should Read This Book

The primary audience for the ASX-400 Auto Sampler OperatorsManua l consists of a na lytical chemists an d lab techn ician s. To use thisman ua l effectively, you should ha ve a st rong knowledge of chemistry, a

basic knowledge of electronic sampling equipment, at least a beginninglevel of computer experience, and working knowledge of an ICP-AES or

ICP-MS instrument.

How to Use This Book

The ASX-400 Auto Sampler Operators Manual contains seven

chapters. You should read the chapters sequential ly the first t ime.Therea fter, refer to th e chapt ers separa tely a s needed. The firstchapter provides an int roduction to the Auto Sampler. Subsequent

chapters detail the primary tasks associated with the ASX-400.

Thism a n u a lcontains the following chapters:

Chapter 1, Introduction, provides you with an overview of theASX-400 Auto Sampler s function a nd design.

Chapter 2, Preparing for Installation, discusses space and powerrequir ements th a t must be met before the ASX-400 is inst alled. It a lsoprovides instructions for unpacking the Auto Sampler.


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ASX-400Auto Sampler Operators Manual

Preface

xiii

Chapter 3, Installing the Auto Sampler, provides step-by-stepprocedures for installing the ASX-400 and connecting i t to theanalytical instrument.

Chapter 4, Verifying Installation, explains how to test thecommunications interfa ce betw een the ASX-400a nd t he host computer.It also explains how to check Auto Sampler components and test thesam ple probe.

Chapter 5, Using the Auto Sampler, describes the tasks youperform during da ily operat ion of the ASX-400.

Chapter 6, Maintaining the Auto Sampler, explains daily,weekly, and periodic maintena nce tasks.

Chapter 7, Troubleshooting the Auto Sampler, describes how todiagnose an d correct ASX-400problems.

These chapters are followed by a glossary of related terms.

Conventions Used in This Book

This book uses certain conventions to distinguish different types ofinforma tion easily. This section describes these convent ions.

Instructions

All step-by-step instructions are numbered and in bold, as in thefollow ing example.

1 Replace the sample vial racks.

Many numbered instructions are followed by more detailedexplanations.


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Preface

xiv

Menu Items

This book uses th e follow ing forma t for referr ing t o menu items:

SettingsCommunication

The text before the arrow symbol is the name of the menu; the textaft er the a rrow sy mbol is the menu choice. This example refers to th e

Communications menu choice in the Settings menu.

Terminology

This book frequ ently uses th e follow ing t erms:

Host computer The computer th a t controls opera tion of the ICP -AESor ICP -MS instru ment t o which the Auto Sampler isa t tached.

Hz Hertz .

ICP-AES An inductively coupled plasma atomic emissionspectrometer.

ICP-MS An ind uctively coupled plasma ma ss spectr ometer.

ID Inside diameter.

LED Light-emitting diode.

PEEK Polyetheretherketone.

VAC Volts a l ternat ing current.

VDC Volts direct curren t.

X-axis The left-to-right axis of the Auto S a mpler.


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Preface

xv

Y-axis The fr ont-to-back a xis of th e Auto S a mpler.

Z-axis The up-a nd-dow n a xis of th e Auto S am pler.

Notes

Notes cont a in a reminder about th e effect of par ticular a ctions. They

ar e indicat ed a s follows:

Note:

This example shows how a note is displayed.

Cautions

Cautions indicate si tuations that require immediate attention toprevent ha rm to the Auto Sampler. Ca utions are indicated as follows:

This exam ple shows h ow a caut ion is displayed.

Warnings

War nings indicat e situa tions tha t could cau se bodily ha rm. War nings

ar e indicat ed a s follows:

This example shows how a warning is displayed.

CAUTION

WARNING


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Preface

xvi

Where to Go for More Information

In a ddition to the ASX-400 Aut o Sampl er Oper ators M anual, you can

refer t o the following resources:

the softw ar e man ua l for the ICP -AES, ICP -MS, or other an alytical

instrument you are using

CE TAC Technologies Cust omer Service a nd Support :

1 (800) 369-2822 (US A only )

1 (402) 733-2829

1 (402) 733-1932 (Fa x)

e-mail: [email protected]


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1

Introduction


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Introduction

The ASX-400 Auto S a mpler is designed t o be sturdy , reliable, an d ea syto use. It provides aut omated sample intr oduction tha t enables you to

perform oth er ta sks while th e Auto Sa mpler run s. The ASX-400a utoma tically int roduces up to 90 sam ples w hen fully loa ded. It

contains a microprocessor that al lows sequential or random sampling,providing flexibility .

The ASX-400 is typically interfaced to and controlled by the ICP-AES,

ICP -MS, or other a na lytical instru ment h ost computer using a serial orIEEE communications protocol.

Auto Sampler Standard Components

Auto sa mpler components a re ma de of corrosion-resista nt sta inless steel

alloys or an odized aluminum. The enclosure a nd base a re made from ahigh-strength aluminum alloy that is chromated and finished with anepoxy powder coatin g.

The ASX-400 operates reliably under a wide variety of conditions.

Components in the sample flow path are made of polyetherimide (PEI)an d polytetra fluoroeth ylene (P TFE ). When these inert, non-meta llic

mat erials are used at tempera tures less tha n 135 C, they can w i thstandrepeat ed exposure to th e follow ing substa nces:

predominan tly aq ueous solutions of str ong acids (less th an 40%)

predomina nt ly aqueous solutions of str ong ba ses (less th a n 10%)

common orga nic solvents such a s a cetone, a lcohols, ethy l aceta te,methylethylketone (MEK), petroleum oils and derived fuels,tetra chloroethy lene, t oluene, a nd xylene


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Introduction

13

Prolonged or repeated exposure to temperatures greater than 135 Cand to the following substances can cause fai lure of the PEI flow path

components:

solut ions of concentr a ted acids (grea ter tha n 40%)

solutions of concentrat ed bases (great er than 10% pota ssium,

am monium, or sodium h ydr oxides)

part ial ly ha logenat ed hydr ocar bons or extremely agg ressive organicsolvents (chloroform, methylene dichloride, 1,1,2-trichloroethane)

Figure 11. ASX-400 Auto Sa mpler Front View .

CAUTION


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Introduction

14

The following standard components are located on the front of theASX-400 a nd a re shipped with th e Aut o Sa mpler. Ea ch lettered item

corresponds w ith a callout in F igure 11.

A Sample tray. The sample tr ay a ccommodat es one sam ple vialra ck. Ribs locat ed on th e bottom of the sample tr ay h old the samplevial rack in place.

B lowing rinse station. The rinse sta tion is loca ted left of center atthe back of the sam ple tra y. It comes with tubing used to connect

the rinse stat ion to the rinse source and t he wa ste container.


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Introduction

15

Figure 12. ASX-400 Auto Sa mpler B ack View.

The following standard components are located on the back of theASX-400 and ar e shipped w ith t he Auto Sa mpler.

Two RS-232 serial I/O ports (COM1 and COM2). The serial portsa re locat ed on th e left side at th e back of the Aut o Sa mpler. The COM1

port is the communications interface between the

ASX-400 and the analytical instrument s host computer. The COM2port conn ects the Auto Sa mpler to oth er externa l devices.


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Introduction

16

The following sta nda rd component s a re a lso shipped wit h t he ASX-400:

External desk-top power supply. The input r a tin g is AC 100V-240V, 1 A, w ith a n out put of DC 24V, 1.7 A. It conta ins a 2 A, 250Vfuse w hich is not user replaceable.

Sample probe kit. The kit includes th e sample probe w ith sa mpletu bing. The sam ple probe fits into the z-drive assembly.

Serial interface kit. The kit includes DB 9F port ada pters for hostcomputer s wit h norma l AT-sty le DTE seria l port s, an d a

1.828-meter modular cable.

Optional Accessories

If you are doing a specialized type of analysis or are connecting the

ASX-400 to a host computer that uses a non-RS-232 communicationsprotocol, you may need optional accessories in addition to the standard

components included w ith t he Aut o Sa mpler. The follow ing a ccessoriesa re a va ilable for th e ASX-400:

IEEE-488 interface kit. The k it in cludes a n RS -232/IE E E -488

converter box, an IEEE-488 cable, a power cord, and instructions.It is used to convert an IEEE communications protocol to a serialprotocol.

Serial interface null adapter. The null ad apt er replaces oneDB 9F port ada pter at t he host computer. It is used for computersw ith D CE -AT sty le seria l port s.

Serial interface special adapter kit. The adapter kit replacesone or both standard serial port adapters with unwired DB9M,DB25M, and DB25F adapters for special applications or hostcomputers with serial ports not conforming to the RS-232 standard.


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Introduction

17

Sample probes. One 0.5-millimeter ID sa mple probe is includedw ith th e ASX-400 Auto Sa mpler. Optional probe sizes a va ilableinclude 0.3 millimeter ID an d 0.8 millimeter I D.

Note:

Contact CETAC Technologies if you need additional accessories notlisted, need added features to integrate the ASX-400 Auto Sampler intoyour analytical system, or have unique requirements. Research anddevelopment of new features and accessories for the ASX-400 AutoSampler, often inspired by customer requests, is a continuing activity of

CETAC Technologies.


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2

Preparing for Installation


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In sta lling the ASX-400 requir es prepar a tion. B efore you insta ll th eAuto Sampler, you should evaluate the physical arrangement of the

labora tory t o choose a suit able location. Once you choose a loca tion,you must carefully unpack the Auto Sampler prior to beginning the

installation.

This chapter discusses what requirements must be met when you

choose a locat ion for the Auto Sa mpler. It a lso describes how to unpack

the ASX-400 before installation.

Choosing a Location

Choosing a location for the ASX-400 involves evaluating the labenvironment for the ava ilabil ity of space, wa ter, an d power. For the

Auto Sampler to function optimally, the location you select must meetspecific requ irements associa ted with ea ch of th ese items. Thefollowing sections discuss space, water, and power requirements.

Space RequirementsMost an alytical a pplicat ions benefit from the sh ortest sample flow path .Therefore, you should place the Auto Sampler close to the analytical

instr ument . The recommended minimum footprint for count ertopinst a llat ion of th e ASX-400 is 43 cm W x 41 cm D x 61 cm H .

Water Requirements

For most applications, deionized water is used as a rinse agent in theASX-400. If a different r inse agent is routinely used, pla ce th e rinse

a gent source with in 2 meters of the ASX-400.


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23

Ensure that there is a l iquid waste receptacle within 2 meters of theASX-400. The w a ste recepta cle inlet should be a t least 30 to 60

centimeters lower tha n t he Auto Sa mpler rinse sta tion outlet .

Power Requirements

P lace th e ASX-400 w ithin 1.2 meters of a power outlet. The Aut o

Sampler s input requirements are 110240 VAC 10%, 50/60 Hz, a nd40 W. Volta ge selection is a ut oma tic.

The ASX-400 uses a n extern al deskt op power supply. The input ra ting

is AC 110V-240V, 1 A w ith a n out put of DC 24V, 1.7 A. It conta ins a 2A, 250V fuse which is not user replaceable.

Ensure that you position the ASX-400 so that the location where thepow er supply cord plugs in to it is easily a ccessible (is not blocked) and it

can be quickly disconn ected if needed.

The power su pply socket is on th e back of the Auto Sa mpler right belowthe power sw itch. Connect th e power supply to the Auto Sampler firsta nd t hen conn ect a line cord to th e power supply. Do not apply pow er

to the power supply un til ready to operate th e Auto Sa mpler.

Unpacking the ASX-400 Auto Sampler

Inspect external packaging upon receipt for holes, tears, smashed

corners, or any other outward signs of damage from rough handling orabuse during shipment. Inspect a l l i tems during unpa cking an d notify

the carr ier immediately of any concealed da mag e.


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24

If t he ASX-400 is shipped or removed from stora ge dur ing cold w eat her,al low the packaged equipment to attain room temperature before

opening and exposing to war m, humid air . It is usually sufficient toprovide 4 to 8 hours for th is purpose.

If condensation forms on or inside the Auto Sampler, allow it to dry

thoroughly before connecting it to a power source and operating it.Fa ilure to do so may cause equipment da mag e.

Remove the packing checklist from the shipping container, and check

off items a ga inst it. Lea ve accessories in the packing unt il you a re

ready to instal l them on the Auto Sa mpler.

Note:

Do not throw away the factory packaging. Keep it for possible futureuse. This is one of the warranty conditions.

CAUTION


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Installing the Auto Sampler


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The ASX-400 Auto Sampler is designed for easy installation.

Insta llat ion consists of two parts: assembling the Auto Sampler andconnecting i t to the host an alyt ical instru ment.

For the most par t, you can insta ll th e ASX-400 w ithout using tools. Infact, using tools such as screwdrivers or pliers to perform most

installation tasks may result in a damaged or unusable instrument.You can remove th umbscrews w ith t ools i f necessary, but do not t ighten

them wi th a nything other than your f ingers .

To install the Auto Sampler, you must complete the following tasks.

Ea ch of these tasks w ill be discussed in detail later in this chapter.

1 Mount the z-drive assembly.

2 Connect the rinse station.

3 Assemble the sample vial rack.

4 Establish external connections.

5 Connect the Auto Sampler to the host computer.

Ensure that AC power is off before proceeding withinstallation.

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Mounting the Z-Drive Assembly

Mounting the z-drive assembly on the Auto Sampler is the first majorta sk. The z-drive assembly must be atta ched to the Auto Sam pler arm

to allow movement an d function of th e sa mple probe. Figur e 31illustr a tes th e z-drive a ssembly component s.

Figure 31. Z-driv e Assembly.


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Attaching the Z-drive Assembly to theAuto Sampler Arm

To attach the z-drive assembly to the Auto Sampler arm, complete thefollow ing st eps, wit h r eference to Figures 3-1 an d 3-2.

1 Position the z-drive assembly at the free end of the Auto

Sampler arm with the z-drive assembly pointing up.

2 Match the 6-x 3-millimeter grooves in the Y-axis slider blockwith the guide rails on the Auto Sampler arm, and slide theblock along the arm tube until the holes in the block alignwith the matching holes in the Y-axis lead screw nut.

3 Secure the Y-axis slider block to the Y-axis lead screw nutusing the 12-millimeter Kynar thumbscrews installed fromthe top (through the bushings).

Take care to only finger t ight en the Ky na r t humbscrews.

Installing the Sample Probe

To insta ll the sa mple probe, complete th e follow ing st eps:

1 Install an O-ring in the slot on the z-axis slider (Figure 31).

2 Install the sample probe through the z-axis slider and thenthrough the O-ring (Figure 31).

3 Move the z-axis slider plus attached sample probe to the top ofthe z-axis drive.


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4 Leave approximately 105 millimeters of the sample probesyellow-colored support tube extending above the top of thez-axis slider (with the slider at the top of the z-axis drive).

5 Verify that the probe tip clears the top of the rinse stationwhen the Auto Sampler is in the home position (Figures 1-1, 3-1)above the rinse station.

You can manually move the Auto Sampler arm with attached z-axisdrive to the rinse station with out dama ge to the Auto Sa mpler.

6 Retain the sample transfer tubing with the spiral-wrap at tie

points approximately 15 and 40 centimeters above the top ofthe z-axis drive, leaving an untangled service loop ofapproximately 13 to 15 centimeters above the probe.

The sample tra nsfer tubing should sti ll have slack remaining w hen th eprobe is at the ma ximum dow nw ar d l imit .


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Figure 32. Z-D rive Assembly w ith Z-Axis Slider.

Do not man euver the sample probe directly a s dam age ma y result .

Note:

To make fine adjustments to the sample probe X/Y targeting, loosen theKynar screw on the probe guide plate 1/8 to 1/4 turn and move the

guide plate up to 0.2 millimeters from the original location. Be sure totighten the Kynar screw before operating the ASX-400.

CAUTION


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37

Assembling and Placing the Sample Vial Rack

The sample vial rack for the ASX-400 Auto Sampler is shippeduna ssembled. However, you can easily assemble the rack without

using tools. Once you assemble the sample vial ra ck, place it in th esample tra y before proceeding w ith t he insta l lat ion.

Before loading or unloading any sample vial racks on thesample tray, park the sampling arm and probe in the homeposition by cycling the power off and on. The home position is

the initial position at power-up. Never attempt to load, unload,or reposition a sample vial rack or sample vial while the AutoSampler is operating.

To assemble and place the sample vial rack, complete the followingsteps:

1 Snap the rack together as shown in the instructions includedwith the rack.

You can also easily disassemble the rack if you need to ship or store

them.

Note:

Keep at least one copy of the assembly instructions provided for eachrack. Keep the copy with this manual for reference.

2 Place the sample vial rack in the middle of the sample tray sothat the feet of the rack underside engage the locating ribs onthe sample trays surface.

Correctly placed sample vial ra cks will not move more tha n 2

millimeters in either a left/righ t or forw a rd/backw a rd d irection un less

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you lift th em. Tilted sample vials indicate a n improperly placed ra ck,w hich must be corrected before you opera te th e Aut o Sampler.

Establishing External Connections

The next step in the installation process involves connecting the

ASX-400 Auto Sampler to the power source and to an analyticalinstr ument . The follow ing sections explain how to esta blish these

connections.

Connecting the Auto Sampler to the Power Source

A voltage-specific external desktop power supply is supplied with each

ASX-400 Aut o S a mpler.

Use only this external desktop power supply or an exactreplacement.

To connect the Auto Sampler to a power source, plug the externaldesktop pow er supply cord in to th e pow er connector located on t he back

pan el of th e Auto Sa mpler. Then, plug the power supplys power cordinto a 100240-VAC 10%, 50/60-H z u tilit y pow er out let.

Connecting the ASX-400 to an Analytical Instrument

You can connect the Auto Sampler directly to a sample introductionperistal t ic pump and then to any sample introduction device, such as

the CETAC U-5000AT+ or U -6000AT+ U ltr a sonic Nebulizer. To do so,complete th e following steps:

1 Determine the length of the sample transfer tubing you need,and cut it to size.

2 Connect the free end of the sample transfer tubing to the inletof the analytical instruments peristaltic pump tubing.

WARNING


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39

Connecting the ASX-400 Auto Sampler to the HostComputer

You cannot operate the ASX-400 Auto Sampler until you establish acommunications interface between the Auto Sampler and the hostcomputer. It is through th is interface that t he host computer directs

th e opera tion of the ASX-400 Aut o Sa mpler. The ASX-400 Aut oSa mpler support s th e follow ing t w o communicat ions protocols:

The seria l (RS-232C) protocol is th e sta nda rd configura tion. There

ar e tw o RS-232C serial ports on th e ASX-400, and a serial int erfa cekit is shipped with the Auto Sampler.

The par a llel (IE EE -488) protocol is less common th a n the serialconfigur a tion. An IE EE -488 interfa ce kit is a va ilable as an optiona l

a ccessory to the ASX-400. See Cha pter 1, Introduction, for moreinforma tion about th is kit .

Note:

Although the ASX-400 supports both serial and IEEE communicationsprotocols, the host computer governs which protocol is used. To

determine which protocol is required for the analytical instrument youare using, refer to the hardware or software manual provided with theinstrument. The following sections explain how to establish acommunications interface using a serial protocol and using an IEEEprotocol.


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When interconnecting any computing devices, keep thecommunications cables away from sources of electromagnetic or radio

frequency (RF) interference, such as electric motors, transformers,fluorescent light balla sts, or RF energy sources. Limit cable run s for

RS-232C t o less tha n 16 meters. If th ese condit ions can not be satisfied,use low-impedance, fully shielded cables to provide satisfactoryopera tion. The ca bles a re ava ilable from ma ny sources, but you w ill

need to specify the correct mating connectors and straight-through (DTE-DCE ) wiring .

Establishing a Serial Communications Interface

The serial interface kit provided with the ASX-400 Auto Samplerincludes an interface cable equipped with two modular port adapters.

Use t he interface kit to establish a serial communicat ions interfa ce withth e host computer. To do so, complete th e follow ing st eps:

1 Plug one end of the cable into the host computers serial (COM)port selected for Auto Sampler communications.

Make sure t ha t t he COM port you select ma tches the port selected in

th e host computer s softw ar e.

2 Finger tighten both screws of the cable adapter.

CAUTION


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Note:

If a host computer serial port with a DB9F, a DB25M, or a DB25Fconnector (9 pin D-submini receptacle or 25 pin D-submini plug orreceptacle) must be used, use the mating connector from the CETACTechnologies universal port adapter kit. You can order the adapter kitfrom CETAC Technologies or purchase an adapter locally to convertthe serial port to a DB9M. Do not use a null modem adapter.

3 Connect the other end of the cable to the ASX-400 COM1 port.

Ensure that you are connecting the adapter to the COM1 port.Connecting the adapter to the Dilutor port on the Auto Sampler will

cause a ma lfunction. The Auto Sa mpler dilutor port is used forcommunications to the Auto Dilutor or other devices other than thehost computer .


Establishing an IEEE Communications Interface

Before you can establish an IEEE communications interface with thehost computer, you must have the IEEE-488 interface kit, which

includes a convert er box a nd a n IE EE -488 cable. U se the IEE E-488 kitto esta blish a par a llel interfa ce w ith th e host computer . To do so,

complete th e following steps:

1 Connect the converter box according to the manufacturersinstructions.

Manufacturer s instructions a re provided with the kit .

2 Plug one end of the cable into the serial port on the 232/488converter box.

CAUTION


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4 Connect the other end of the cable to the ASX-400 COM1 port.

Ensure that you are connecting the adapter to the COM1 port.Connecting the adapter to the Dilutor port on the Auto Sampler willcause a ma lfunction. The Auto Sa mpler dilutor port is used for

communications to the Auto Dilutor or other devices other than thehost computer .


CAUTION


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4

Verifying Installation


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Once installation of the ASX-400 Auto Sampler is complete, it isimporta nt t o verify that you have instal led it correctly. Attempting to

use i t before ensuring th at i t is instal led correctly may result in dama geto the Auto Sampler.

Verifying installation of the Auto Sampler consists of two parts:

ensuring that th e communications interface between it an d the host

computer is w orking properly.

ensur ing th a t th e sam ple probe functions properly.

This cha pter explains h ow t o test th e above items before using th e AutoSampler.

Note:

The procedures given in this chapter are for use in a Windows195 or

WindowsNT environment.

Testing the Interface

If t he communications interface between t he Auto Sa mpler a nd t he hostcomputer is not established correctly, the Auto Sampler will not

function. B efore you test the interface, ensure that the communicationport connectors are properly attached between the host computer and

the Auto Sampler.

1Windows is a registered trademark of Microsoft Corp.


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Note:

The following procedures assume that you have opened Windows95or WindowsNT and the Program Manager window is showing.

To test t he communicat ions int erfa ce, complete th e follow ing st eps:

1 Start the host computer and go to the main Windowsscreen.

2 Turn on power to the Auto Sampler.

3 Click the start button in the lower left corner of the ProgramManager window.

A selection list w ill appear.

4 Select Programs>>>Accessories>>>Hyperterminal and double-click.

The H yperterminal 1w indow appears (Figure 41).

Figure 41. Exa mple of Hypertermina l Window.

1Hyperterminal is a registered trademark of Hilgraeve, Inc.


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5 Double click on the Hyperterminalicon.

The C onnection D escription box appear s (Figure 42).

Figure 42. Connection Description B ox.

6 Type the name COM1_test for the connection and choose anicon from the list given, and click OK.

The phone number box appears (Figure 4-3).

Figure 4-3. P hone Number B ox.


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7 Select Direct to COM1 in the Connect Using box. Click OK.

The COM1 Properties box appears (Figure 4-4).

Figure 4-4. COM1 Properties Box.

8 Change the bits per second to 9600, set the data bits to 8, theparity to none, the stop bits to 1, the flow control to none, andclick OK.

9 Select File>>>Properties.

The COM1_Test Properties box appears.

10 Select Settings on the COM_Test Properties box and click onthe ASCII setup button on the lower right of the box.

The ASCII setup box appears (Figure 4-5).


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Figure 4-5. ASCI I Setup B ox.

11 In the ASCI I Setup box, select the following items:

a. Echo typed chara cters locally.

b. Append line feeds to incoming line ends.

c. Wra p l ines tha t exceed terminal w idth.

Click OK.

12 Click OK on the COM1_Test Properties box.

13 Type HOME at the cursor in the upper left of the mainHyperterminalscreen and press Enter.

The Auto Sampler resets, with the sample probe moving out and backinto the home position. If th e Auto Sa mpler does not reset, see Ch a pter

7, Troubleshooting th e Auto Sa mpler.


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Checking the Auto Sampler Components

The following Auto Sampler components may be damaged during

shipping or insta llation: th e sam ple probe, the perista ltic pump tubing,an d the rinse sta tion and tubing. It is importa nt th at you check thesecomponents for da ma ge before you opera te th e Aut o Sampler. To do so,

complete th e following steps:

1 Shut down and unplug the Auto Sampler.

2 Visually inspect the sample probe, peristaltic pump tubing, andrinse station and tubing for leaks or signs of damage.

If you detect a leak or other damage to an Auto Sampler component,you must replace it. For more informa tion, see th e appropria te section

in Cha pter 6, Maint aining th e Auto Sam pler.

Testing the Sample Probe

The sample probe must descend into the center of each sample vial to

ensure satisfactory sample uptake. Shipping or rough ha ndling can

disturb the Auto Sampler s cabinet-to-base a lignment . If it isincorrectly a ligned, the sa mple probe w ill not function properly. It istherefore important to test the sample probe before you actually runsamples with t he Auto Sa mpler.

Note:

Before testing the sample probe, ensure that you have installed all AutoSampler components correctly. Also, ensure that you have securelytightened all thumbscrews and connected the communications cablefrom the host computer to the COM1 port on the Auto Sampler.


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Testing the sample probe involves observing the operation of thesa mple probe. To do so, complete th e following st eps:

1 Load the Auto Sampler sample tray with an empty sample vialrack.

For information about placing the sample vial rack, see Chapter 3,

Insta l ling th e Auto Sa mpler .

2 Turn the Auto Sampler power switch on and verify that theLED power indicator is on.

The LE D power ind ica tor is green in color. The indicat or is locat ed

behind th e z-drive a ssembly wh en it is in the home position.

3 Using the host computer control software, designate samplepositions at the left rear, left front, right rear, and right front ofthe sample vial rack.

4 Place the sample vial rack and sample vials at the designatedpositions.

5 Command the Auto Sampler to move the sample probe to thedesignated sample positions. Check that the sample probecorrectly accesses each position and that the probe descends

into the center of each sample vial.

Note:

If the Auto Sampler alignment is not correct, contact CETACTechnologies Customer Service and Support or an authorizedrepresentative.


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Using the Auto Sampler


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The ASX-400 Aut o Sam pler is both r eliable an d easy to use. B eforeusing i t , however, ensure tha t y our lab environment provides operat ing

conditions t ha t w ill prolong t he life of th e ASX-400 Auto Sa mpler. Oncethe proper operating conditions are met, you can place the sample vial

ra cks and sta rt t he Auto Sa mpler sequence run. When you finish usingthe Auto Sampler, you may need to flush the rinse station and flowpath before shutt ing the Auto Sampler down .

This chapter explains how to create the proper operating conditions forusing th e ASX-400 Auto Sam pler. It a lso expla ins how to sta rt a ndshut down t he Auto Sampler, and flush the rinse sta tion a nd flow pa th.

Establishing Optimal Operating Conditions

The ASX-400 Auto S a mpler opera tes r elia bly even un der less th a n idea lconditions. It is not, how ever, indestru ctible. Ma lfunction or da ma gecan occur if specific opera ting conditions a re not met. Meeting th ese

conditions requires that you create the proper lab environment, replaceAuto Sampler components that wear out under normal use, and

purcha se th e a ppropria te supplies for use w ith th e Auto Sam pler. Thefollow ing sections explain how to meet t hese condit ions.

Note:

Damage or malfunction that results from unsatisfactory operatingconditions may constitute misuse and abuse and be excluded fromwarranty coverage.

Creating the Lab Environment

To create satisfactory operating conditions in your lab environment,follow th ese guidelines:


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Operat e the ASX-400 Auto Sa mpler in a conventiona l la benvironment where the temperature is 5095 F (1035 C), the

humidity is 2070%non-condensing, and the unit is not exposed toexcessive fla mma ble or corrosive mat erials.

Avoid roug h han dling of th e ASX-400 Aut o Sa mpler. If possible, donot expose the Auto Sa mpler to vibrat ion or shock.

P rotect th e Auto Sampler from long-term exposure to conden sat ion,

corrosive materials, solvent vapor, continual standing liquids, orlar ge spills into the Auto Sa mpler cabinet or ar m. Exposures of this

type can da mag e the drive mecha nisms as w ell as t he electronics.

Observe the same genera l electrosta tic discha rge precau tions as

with an y other integrat ed circuit electronic devices. Low humidityenvironments, especially when combined with static-generating

mat erials, require maximum care.

Discharge static buildup and ground to the Auto Sampler baseor cabinet before performing any maintenance. Do not touchor short-circuit bare contacts, COM1, Dilutor, or auxiliaryports.

Avoid using t he ASX-400 Auto Sa mpler if strong electr omagn eticinterference, radio frequency interference, or radioactivity is

present. In terferen ce fields ca n cause erra tic opera tion of the Aut oSa mpler. The Aut o Sampler w ill not fun ction properly if the level ofra dioactivity is above background.

Replacing Auto Sampler Components

The following ASX-400 Auto Sampler components wear out undernormal use and must be replaced periodically.

perista ltic pump tubing

sam ple probe

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If you fail to replace these components when they deteriorate, the AutoSa mpler w ill not function properly. For informa tion a bout replacing

Auto Sampler components, see Chapter 6, Maintaining the AutoSampler.

Purchasing Supplies

Because the l i fe-span of the sample and standards vials varies, youshould ma inta in an ad equat e supply of spar e vials. When you need to

purchase additional supplies, it is extremely important that you choosethe a ppropriate sizes an d ma terials.

When y ou purchase sample an d/or sta nda rds vials, ma ke sure theymeet the following requirements:

The diameter of the sample or stan dar ds vial mat ches the rack size

you ar e using. The diamet er of the stan da rds vial is 28 millimeters,plus or minu s 1 millimeter.

The heigh t does not exceed 125 millimeter s.

Via ls used for sta nda rds are 50-mL conica l-bottom cent rifuge via ls.Anyt hing other th an ta pered-bottom via ls wil l not r emain properly

positioned in the rear sta nda rds ra ck.

The material is compatible w ith the samples you ar e an alyzing orthe reference stan da rds you are using. This requirement alsoapplies to the peristaltic pump tubing.

Use of mismatched sample vials and sample vial racks mayresult in malfunctions or sample spills. Be sure your vials meetthe given requirements.

To order additional supplies, refer to the CETAC Accessories and

Suppl ies Catal ogfor t he ASX-400 Aut o Sa mpler.

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Starting the Auto Sampler

Once you install the sample vial rack and ensure that i t is correctly

defined in the software, you can start the Auto Sampler and let i t rununt il th e sa mpling sequen ce is finished. To do so, complete th efollow ing steps:

1 Ensure that the rinse station is properly connected.

For more information about proper connections, see Chapter 3,

Insta l ling th e Auto Sa mpler.

2 Turn the ASX-400 Auto Sampler power switch on.

The gr een LED indicat or along th e Auto Sa mpler x-axis l ights up w hen

th e pow er is on.

3 Adjust the peristaltic pump shoe until the desired rinsesolution flow rate is achieved.

4 Purge air from the rinse system by placing the rinse solutionuptake tubing in the rinse solution source and running therinse solution through the rinse station.

Ensure there are no air bubbles visible in the rinse uptake tubingbefore you r un sa mples with th e ASX-400 Auto Sa mpler.

Note:

If you are flushing the rinse system during initial startup, first use a 2%nitric acid solution as the rinse agent. Flush the rinse system a secondtime using deionized water as the rinse agent.

5 Access the host computers software and activate the AutoSampler program.

The ASX-400 Auto Sampler runs until it reaches the end of thesampling sequence.


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Shutting Down the Auto Sampler

To shut d own th e Auto Sa mpler, complete the following st eps:

1 Drain the rinse system by removing the rinse solution uptaketubing from the rinse solution source. Let the peristaltic pumprun until all solution drains from the tube attached to the rinsestation outlet.

If you use a rinse solution other than deionized water, flush the rinse

system with deionized water before shutting down the ASX-400 AutoSa mpler. For more informa tion, see th e follow ing section, Flushingthe Rinse Stat ion a nd Flow Pa th.

2 Release the pressure shoe on the peristaltic pump.

Releasing t he pressure shoe decreases w ear on the pump t ubing.

3 Turn off the ASX-400 Auto Sampler power switch.

Flushing the Rinse Station and Flow Path

Generally, you can operate the ASX-400 Auto Sampler without flushingthe rinse system. Un der norma l circumsta nces, you can simply drain

the rinse system prior to shutting down t he Auto Sampler. However,you need to flush the rinse station and flow path under two

circumstances:

dur ing initia l star tu p of the ASX-400 Auto Sa mpler a fter

installation

follow ing the use of strong bases, acids, or orga nic solvents as rinse

agents


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Flushing the rinse system during initial startup of the ASX-400 AutoSampler removes any contaminants that could cause interference

during sample an alysis. Flushing the rinse system after using strongrinse agents prevents degradation and fai lure of the flow pathcomponents.

To flush t he rinse sta tion an d flow pat h, complete the following st eps:

1 Insert the rinse uptake tubing into a deionized water source.

Note:

If you are flushing the rinse system during initial startup, first use a 2%nitric acid solution as the rinse agent, followed by deionized water.

2 Run the rinse solution through the rinse station and flow pathfor 5 to 10 minutes.

Once you flush the rinse system, you can proceed with the samplingsequence or dra in the rinse system a s part of the shutdown procedure.For information about running the sampling sequence, see Star t ing

the Auto Sampler earl ier in this cha pter. For more information aboutdraining the rinse system, see the previous section, Shutt ing Down the

Auto Sampler.


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Maintaining the Auto Sampler


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Routine maintenance of the ASX-400 Auto Sampler consists of dailyan d w eekly clea ning of specific Auto Sa mpler components. Routine

maintenance also includes checking ASX-400 components for leaks orother dama ge. Additiona l periodic maint enan ce ta sks may be required,

including replacement of the following Auto Sampler components:peristal t ic pump tubing, sa mple probe, rinse sta tion tubing, a nd samplet ray .

This cha pter explain s how to clea n th e ASX-400 Aut o Sam pler, inspectit for leaks, and replace damaged components.

Discharge static buildup and ground to the Auto Sampler baseor cabinet before performing any maintenance. Do not touchor short-circuit bare contacts, COM1, Dilutor, or auxiliaryports.

Cleaning the Auto SamplerCleaning the ASX-400 Auto Sampler is the primary maintenance task

you perform. Fa ilure to do so regularly can cause increased wear a ndreduce the Auto Sam pler s opera tiona l life.

You must clean the ASX-400 Auto Sampler both daily and weekly to

prevent da mag e an d extend i ts operational l ife. It is especiallyimportant to clean up spil ls and remove contaminants, such asabrasives, from the Auto Sampler s moving par ts. It may also be

necessary to chemically neutra lize spills. The follow ing sectionsexplain d aily a nd weekly cleaning procedures.

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Daily External Cleaning

Use of the ASX-400 Auto Sampler often results in spills on AutoSa mpler components such as the sample tra y. G ood ma intenan ce

requir es tha t you clean th e Aut o Sa mpler daily. To do so, complete th efollow ing steps:


For informa tion about shutt ing down the Auto Sampler, see Cha pter 5,

Using t he Auto Sa mpler.

2 Wipe the sample tray, Auto Sampler cabinet, and Auto Samplerarm using a towel dampened with a lab-grade cleaning agent.

Do not al low the cleaning agent to come into contact with the leadscrews. Also, never lubrica te either of th e two lead screws.

3 Repeat step 2, using a towel dampened with clear water.

This process removes any remaining contaminants.

4 Dry the sample tray, Auto Sampler cabinet, and Auto Sampler

arm using a dry towel.The ASX-400 Auto S a mpler must be thorough ly dr y before you tur n t he

Auto Sa mpler pow er on.

Weekly Cleaning

Although daily cleaning removes spills and contaminants from most of

the Auto Sampler components, it is necessary to clean the ASX-400Auto Sa mpler more thoroughly once a w eek. To do so, complete thefollow ing steps:

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2 Remove the sample tray.

For information about removing the sample tray, see Replacing theSample Tray later in th is cha pter.

3 Wipe loose particles off the Y-axis lead screw with a dry,lint-free cloth.

The Y-axis lead screw is a large metal screw located inside the AutoSa mpler arm t ube, as shown in Figure 61.

Figure 61. Z-D rive Assembly on Aut o Sa mpler Arm .


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Never lubricate the lead screws. The lead screw nuts arecompounded with a dry film lubricant. Oiling the lead screwswill cause gumming, galling, and binding of the sample probeassembly.

4 Wipe the Auto Sampler exterior and base until they are clean,using a towel dampened with a lab-grade cleaning agent,followed by a towel dampened with clear water.

P ay special a ttent ion t o the sl ider block and gu ide rails along t he tube

of the Auto Sa mpler a rm.

5 Wash the sample tray in a warm detergent solution.

Make sure y ou remove all spills and sta ins.

6 Rinse the sample tray with water and then dry it.

Ensur e that t he sample tra y is thoroughly dry.

7 Replace the sample tray on the Auto Sampler base.

For information about replacing the tray, see Replacing the SampleTray later in th is cha pter.

Checking for Leaks

Several of the Auto Sampler components have a limited life and willwea r out under normal use: the sample probe, the peristal t ic pump

tubing, and the r inse s ta t ion and tubing. S ta ndard maintenanceprocedures require that you periodically check these components for

leak s. To do so, complete th e followin g steps:

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Replacing the Sample Probe

You must replace the sample probe if it is leaking or shows other signs

of det eriora tion. To do so, complete the followin g steps:


2 Remove the old sample probe and tubing.Be careful not to use excessive force when removing the sample probe.Applying too much force can result in da ma ge to the z-drive a ssembly.

3 Remove the old O-ring and replace it with a new O-ring.

4 Install the new sample probe.

For information about instal l ing the sample probe, see Chapter 3,


5 With the z-drive in the full-up position, hold the z-axis sliderand move the sample probe tube up and down so that 3 to 6

millimeters extends below the sample probe guide plate.

Replacing the Rinse Station Tubing

If the rinse station tubing is typically exposed to deionized water as arinsing a gent , you do not need to replace it often. How ever, if you use

other rinsing agents, such as acids or solvents, the tubing is likely todeteriora te more rapidly. To replace th e rinse sta tion tubing, complete

th e follow ing st eps:


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2 Move the Auto Sampler arm 20 to 30 centimeters away from thehome position by gently pushing it.

Moving the Auto Sampler arm ensures that the sample probe will not

be dama ged wh ile you replace the rinse station tubing.

3 Disconnect the rinse solution uptake and drain tubing.

Apply only a linear force when removing the tubing to prevent thefi t t ings from breaking.

4 Remove the rinse station tube by completing the followingsteps:

a Rotate the rinse station tube counterclockwise 1/4 turn.

b Remove the rinse station tube from the mounting block bylifting the tube straight up.

5 Replace the rinse station tube by pushing the new rinsestation tube into the mounting block and rotating it clockwise1/4 turn.

6 Reconnect the rinse solution uptake and drain tubing.

Apply only a linear force when replacing the tubing to prevent thefi t t ings from breaking.

7 Move the Auto Sampler arm back to the home position.

Replacing the Sample Tray

Cleaning the ASX-400 Auto Sampler sample tray each week extends itsl ife an d makes frequent replacement unn ecessary. However, if the

sam ple tr a y n eeds to be replaced, complete the following steps:


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2 Remove the sample vial rack.

3 Move the Auto Sampler arm 20 to 30 centimeters away from thehome position by gently pushing it.

Moving th e Auto Sa mpler a rm ensures t ha t t he sam ple probe assemblywill not be dam aged w hile you replace the sam ple tra y.

4 Raise the rinse station tube approximately 2 centimeters.

5 Raise the front edge of the damaged tray at least 2.5centimeters and slide it forward.

If you have difficulty removing the sample tray, raise the front edge

higher before sliding it forward.

6 Install the new tray.

7 Lower the rinse station tube.

Ensure the rinse station tube is positioned securely.

8 Move the Auto Sampler arm back to the home position.

9 Replace the sample vial rack.


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7

Troubleshootingthe Auto Sampler


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Troubleshooting the Auto Sampler

The ASX-400 Auto Sampler is both easy to operate and reliable.How ever, problems wit h it may occur. When the Auto Sam pler does

not function properly, isolate the problem to determine if it originatesin the host computer, the analytical instrument, the RS-232 cable, or

th e Auto Sa mpler. If you deter mine th e problem is in th e ASX-400,check the power system, the communications interface, or the sampleprobe a ssembly to find th e cause of the problem a nd r esolve it.

This chapter explains how to troubleshoot ASX-400 Auto Samplerproblems. If you cann ot solve a problem using the steps given in thischapt er, cont a ct CE TAC Technologies Customer S ervice and S upport.

Power System Problems

A possible cause of ASX-400 Auto Sampler malfunction is a problem inthe pow er system. If th e Auto Sa mpler is not functiona l , there may beno power getting to it . If this is the case, the green LED power

indicat or will be off. To troubleshoot this problem, complete thefollow ing steps:

1 Check the wall outlet and see if the external power supply isplugged in.

2 Check that the power switch of the external power supply isturned on.


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Interface Problems

Operation of the ASX-400 Auto Sampler is directed by the host

computer . A ma lfunction of the Auto Sa mpler ca n indicate a problemwith the RS -232 cable or w ith t he configurat ion of the softw ar e on t hehost computer . The follow ing sections explain how to troubleshoot

th ese problems.

RS-232 Cable Problems

The first step in troubleshooting interface problems is to check theRS -232 ca ble. To do so, complete th e followin g st eps:

1 Check the Auto Sampler power switch to ensure it is on.

2 Check the RS-232 cable to ensure it is plugged in to the COM1port on the Auto Sampler.

If th e cable is plugged in, ensure th at i t is t ightened properly.

3 Check the host computer to ensure that the RS-232 cable isconnected to the appropriate COM port.

If th e cable is plugged in, ensur e th a t it is tigh tened properly. For moreinformation about connecting the RS-232 cable, see Chapter 3,


Software Configuration Problems

If the RS-232 cable is connected properly and the Auto Sampler is stillnot communicating with the host computer, ensure that the host

softwa re is configur ed correctly. To do so, complete the follow ing st eps:

1 Run the host software and ensure that the instrument isfunctioning properly.


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2 Check the software configuration for the correct COM portselection and baud rate (9600, N, 8, 1).

If the wrong port or baud rate is selected, change the configuration.

For information about changing the software configuration, seeCha pter 4, Verifying Insta llat ion.

Z-Drive Assembly Problems

An ASX-400 Aut o Sa mpler ma lfunction ma y be ca used by a problem in

the z-drive assembly. You can easily determine tha t a m alfunction isrelated to t he z-drive assembly i f you h ear a loud chat tering noise wh enthe ASX-400 power switch is on or if the sample probe is not moving.To troubleshoot z-drive assembly problems, complete the following

steps:

1 Ensure that the Y-axis slider block and z-drive assembly areinstalled.

If the z-drive assembly is not installed, follow the instructions provided

in Chapter 3, Insta l ling th e Auto Sa mpler, to insta ll it. If the z-driveassembly is a lready insta l led, continue w ith st ep 2.

2 Check the Y-axis block home position flag for damage.The home position flag is shown in Figure 71. If the flag is dama ged,you must replace the entire y-a xis slider block. For informa tion aboutmounting the y-axis slider block on the Auto Sampler arm, see

Cha pter 3, Insta l ling th e Auto Sa mpler.

If t he home position fla g is und ama ged, continue w ith step 3.


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Figure 71. Z-Dr ive Assembly wit h Y-Axis Block Home Position Flag .


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3 Check that the sample probe is moving.

If t he sa mple probe is binding, fr ee the sa mple probe assembly.

Note:

If you cannot free the z-drive assembly, you will need to replace it. SeeChapter 3, Installing the Auto Sampler,for information about mountinga new z-drive assembly. You can order a new z-drive assembly from

CETAC Technologies.


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Glossary


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Glossary

This glossary defines the terms used in the ASX-400 Auto Sampler

Operators M anual .

analytical instrument Theinstrument, t ypically a n ICP -AES

or ICP-MS, to which the AutoSa mpler is conn ected.

Auto Sampler arm The a rmtha t extends from the front of theASX-400 cabinet. It governs thelef t / r ig h t a n d for w a r d /

backward travel of the z-driveassembly.

I/O ports The connections usedfor establishing communicationbetween the ASX-400 or the ADX-

100 and the host computer orother external devices.

peristaltic pump The on-boar dpump controlling the movement

of the rinse solution to the rinsestation.

rinse solution The solution,typically deionized w at er, used to

clean th e sample probe.

rinse station The Auto Sam plercomponent used to clean thesample probe with a rinse

solution.

Y-axis slider block Thecomponent that fits over the Auto

Sampler arm and executes theforwa rd/backw ar d movement of

the z-drive assembly along the

Auto Sa mpler a rm.

Z-axis rotor The componen ttha t controls the movement of the

Z-axis slider.

Z-axis slider The z-driv eassembly component tha t g overnsthe up-and-down travel of the

sa mple probe.

Documents

ASX-400 Operators Manual