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2007-2008 AP Biology Prokaryotes Domain Bacteria Domain Archaebacteria Domain Bacteria Domain Archaea Domain Eukarya Common ancestor

AP Biology 2007-2008 Prokaryotes Domain Bacteria Domain Archaebacteria Domain Bacteria Domain Archaea Domain Eukarya Common ancestor

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  • AP Biology 2007-2008 Prokaryotes Domain Bacteria Domain Archaebacteria Domain Bacteria Domain Archaea Domain Eukarya Common ancestor
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  • What characteristics make Archaea more like eukaryotes than prokaryotes? No peptidoglycan in cell wall More than 1 RNA polymerase Some introns Met is 1 st amino acid in protein synthesis Ribosomes more like eukarytoes (Streptomycin does not kill archaea) Domain Bacteria Domain Archaea Domain Eukarya Common ancestor
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  • Bacteria live EVERYWHERE! Bacteria live in all ecosystems on plants & animals in plants & animals in the soil in depths of the oceans in extreme cold in extreme hot in extreme salt on the living on the dead Microbes always find a way to make a living!
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  • Bacterial diversity rods and spheres and spirals
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  • Prokaryote Structure Unicellular bacilli, cocci, spirilli Size 1/10 size of eukaryote cell 1 micron (1um) Internal structure: prokaryote cell eukaryote cell
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  • Prokaryote vs. Eukaryote Chromosome double helix Prokaryote Eukaryote
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  • Genetic variation in bacteria Mutations bacteria can reproduce every 20 minutes binary fission error rate in copying DNA 1 in every 200 bacteria has a mutation you have billions of E. coli in your gut! lots of mutation potential! Genetic recombination bacteria swap genes small supplemental circles of DNA direct transfer of DNA conjugation
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  • Metabolic Relation to Oxygen How are bacteria categorized by the effect that oxygen has on growth? Obligate aerobes Facultative anaerobes Obligate anaerobes What role do prokaryotes play in nitrogen cycling? Only way that nitrogen gas can be incorporated into organic molecules
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  • Variations in Cell Interior internal membranes for photosynthesis like a chloroplast (thylakoids) internal membranes for respiration like a mitochondrion (cristae) aerobic bacterium mitochondria cyanobacterium (photosythetic) bacterium chloroplast
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  • Origins of Metabolic Diversity Heterotrophs most likely came before photoautotrophs (parsimony) Glycolysis was probably the first metabolic pathway Natural selection favored autotrophs as heterotrophs depleted food supply Cyanobacteria introduce chl a and oxygen gas.
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  • Cell Wall What are the functions of the cell wall in prokaryotes? Maintain cell shape Protection Prevent bursting in hypotonic environment Made of peptidoglycan Why is some food preserved by salting it? Hypertonic environment plasmolyzes bacterial cells
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  • Prokaryote Cell Wall Structure peptide side chains cell wall peptidoglycan plasma membrane protein Gram-positive bacteria Gram-negative bacteria peptidoglycan plasma membrane outer membrane outer membrane of lipopolysaccharides cell wall peptidoglycan = polysaccharides + amino acid chains lipopolysaccharides = lipids + polysaccharides Thats important for your doctor to know!
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  • Prokaryotic metabolism How do bacteria acquire their energy & nutrients? photoautotrophs photosynthetic bacteria chemoautotrophs oxidize inorganic compounds nitrogen, sulfur, hydrogen heterotrophs live on plant & animal matter decomposers & pathogens
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  • Disease Disease exotoxins release poisons such as botulism and cholera Endotoxins seen in outer membrane of gram neg. bacteria (Salmonella) What are Kochs postulates and why are they important? Kochs postulates used to isolate pathogen causing disease
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  • Bacteria as pathogens Disease-causing microbes plant diseases wilts, fruit rot, blights animal diseases tooth decay, ulcers anthrax, botulism plague, leprosy, flesh-eating disease STDs: gonorrhea, chlamydia typhoid, cholera TB, pneumonia lyme disease
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  • Bacteria as beneficial (& necessary) Life on Earth is dependent on bacteria recycling of nutrients from dead to living only organisms that can fix N from atmosphere needed for synthesis of proteins & nucleic acids plant root nodules help in digestion (E. coli) digest cellulose for herbivores cellulase enzyme produce vitamins K & B 12 for humans produce foods & medicines from yogurt to insulin
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  • Germinating akinetes
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  • AP Biology 2007-2008 Got any Questions?? Ask da Boss!
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  • Are these bacteria Gram + or Gram ? Gram positive Gram negative