Answer Sheet Corn Germination Referee: Seedling Evaluation Arbitre de germination SPR de maïs

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Answer Sheet Corn Germination Referee: Seedling Evaluation Arbitre de germination SPR de mas Slide 2 Red data indicate the error rates Picture No. AOSA (51 participants)M&P (46 participants) AnswerNormal (+)Abnormal (-)unknownAnswerNormal (+) Abnormal (-) 1 - 1338 + 9 25%75%83%20% 2 - 1437 - 2224 27%73%48%52% 3 + 22281 + 1333 43%55%2%28%72% 4 + 510 + 451 100%0%98%2% 5 + 37131 + 2719 73%25%2%59%41% 6 - 1536 + 2917 29%71%63%37% 7 - 744 - 145 14%86%2%98% 8* Photo may not clear - 3714 - 3511 73%27%76%24% 9 - 744 - 1135 14%86%24%76% 10 - 744 - 2 14%86%4%96% 11 + 429 + 388 82%18%83%17% 12 + 510 + 433 100%0%93%7% Slide 3 1. Note: Split entire length of coleoptile to node In accordance with M&P this would be classified as Normal Leaf is not badly shredded or longitudinally split There is not a deep open crack in the Mesocotyl AOSA and ISTA: Abnormal: Leaf damaged, coleoptile tip damaged and the coleoptile split more than 1/3 of the length from the tip. Slide 4 2. Abnormal: Missing primary root with weak seminal roots. Note: Mixed opinions whether the seminal roots are weak, however, the majority of participants concluded seminal roots are insufficient. This may need further clarity or guidelines in rules for achieving uniformity in evaluation result. Slide 5 3. Normal: Decay in middle of coleoptile is from secondary infection, e.g., test conditions either temperature too warm in towel, or decayed seedling adjacent. Tissue above and below decayed area is healthy indicative of secondary infection. Decay Slide 6 4. Normal In towels, there may be considerable twisting of the shoot system. The shoot is twisting around another seedling in the towel to reach the light or the seed could have been upside down. Seedlings grown in the dark or in low intensity light will exhibit increased elongation of the coleoptile and Mesocotyl (not this photo). Slide 7 5. Normal: The leaf extends more than way up in the coleoptile. Hint: Knowing the separation node between the mesocotyl and coleoptile. Slide 8 6. Normal for M&P Leaf is not badly shredded or longitudinally split, no deep crack in the mesocotyl. Abnormal for AOSA and ISTA - Leaf damaged, coleoptile tip damaged if the first leaf has emerged at time of evaluation, seedling is abnormal if the coleoptile has any of the following defects together with damage to the first leaf --coleoptile split for more than one-third of the length from the tip. --coleoptile strongly bent over. --coleoptile tip damaged or missing. --coleoptile split at any location below the tip. Slide 9 7. Abnormal: The leaf extends less than way up the coleoptile. Slide 10 8. Abnormal: Split in coleoptile - visible from the beginning of spiral, and down through mesocotyl. Tight spiral is considered as normal in rules, but a close exam of the essential structures is necessary, e.g., deep split in mesocotyl. Note: Photo may not show clearly the crack in the mesocotyl. split Slide 11 9. Abnormal : Deep open crack in Mesocotyl Slide 12 10. Abnormal Seedling is weak, stunted at the end of test duration appears to be some browning at point of attachment. Stubby primary root with weak seminal roots. browning Slide 13 11. Normal Two shoots from one seed are normal as long as they are not fused together and/or provided one is normal with no seedling structures impaired. See M&P: 4.12.6 Slide 14 12. Normal sufficient growth all essential structures are visible and no defects, - healthy shoot and root