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Agilent 1290 Infinity LC Next Generation Technology for highest Performance and Flexibility. HPLC 2009 HPLC 2009 Dresden, Germany Stefan Schuette Agilent Technologies Agilent Technologies

Agilent 1290 Infinity LC - Chemical Analysis, Life … 1290 Infinity LC Power Range ¾All analytical columns ¾Method Transfer 1200 bar ¾Higher Resolution ¾Faster Analysis ¾Higher

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Page 1: Agilent 1290 Infinity LC - Chemical Analysis, Life … 1290 Infinity LC Power Range ¾All analytical columns ¾Method Transfer 1200 bar ¾Higher Resolution ¾Faster Analysis ¾Higher

Agilent 1290 Infinity LC

Next Generation Technology for highest Performance and Flexibility.

HPLC 2009HPLC 2009Dresden, GermanyStefan SchuetteAgilent TechnologiesAgilent Technologies

Page 2: Agilent 1290 Infinity LC - Chemical Analysis, Life … 1290 Infinity LC Power Range ¾All analytical columns ¾Method Transfer 1200 bar ¾Higher Resolution ¾Faster Analysis ¾Higher

Short History of UHPLC

2003 Agilent 1200 Binary 1.8um RRHT Columns2004 Waters Acquity 1.7um BEH Columns2006 Agilent 1200 RRLC2006 Agilent 1200 RRLC

Thermo Accela 2007 Shimadzu UFLCxr

Jasco Xtreme-LCHitachi LaChrom UltraScient. Syst. Ultra HPy

2008 Dionex RSLCKnauer Platin Blue

2009 PE Fl2009 PE Flexar

10 New UHPLC Systems in 5 Years – All different.

Page 3: Agilent 1290 Infinity LC - Chemical Analysis, Life … 1290 Infinity LC Power Range ¾All analytical columns ¾Method Transfer 1200 bar ¾Higher Resolution ¾Faster Analysis ¾Higher

UHPLC Situation 2009Power Rangesg

1200

bar Power = Pressure x Flow

Pressure Resolution

1000WatersAcquity

Pressure ResolutionFlow Speed

600

800Acquity

Dionex RSLC

400

Agilent RRLCThermoAccela

Shimadzu UFLCXR

0

200

Standard LC

3

0 1 2 3 4 5 ml/min

Page 4: Agilent 1290 Infinity LC - Chemical Analysis, Life … 1290 Infinity LC Power Range ¾All analytical columns ¾Method Transfer 1200 bar ¾Higher Resolution ¾Faster Analysis ¾Higher

UHPLC Situation 200910 Systems in 5 Years – All Different!y

Problems

• Method Transfer

Differences

• System Volumes

Debate

• Methods: transferable?• Not possible• Requires re-devel.

and -validationP f Li it ti

y

• Pressures

• Flow Rates

T t

• ID: 4.6, 3 or 2.1 mm?

• Particle: TP or SP?• Performance Limitations

• Speed • Resolution • Sensitivity

• Temperatures

• Data Rates

• Sensitivity

• Size: STM or larger?

• Flow: low or high?

• T: low or high?• Sensitivity • Precision• Linearity

• Injection principle

• ...

• T: low or high?

• P: 600 or 1000 bar?

4

STM = Sub-2um SP = Superficially PorousTP = Totally Porous

Page 5: Agilent 1290 Infinity LC - Chemical Analysis, Life … 1290 Infinity LC Power Range ¾All analytical columns ¾Method Transfer 1200 bar ¾Higher Resolution ¾Faster Analysis ¾Higher

Agilent 1290 Infinity LC- Development Objectives- Development Objectives

System Requirements The Debate is Over

1. Power Range:encloses any (U)HPLC

• Methods: transferable !

• ID: 4 6 3 and 2 1 mm !2. Delay Volume:

lowest, adjustable

3. Sensitivity, Precision:

ID: 4.6, 3 and 2.1 mm !

• Size: STM and larger !

• Particle: TP and SP !better than any (U)HPLC

4. Injection Principle:variable and fixed loop

a c e a d S

• Flow: low and high !

• T: low and high !

5. Gradient Behaviour:emulate any (U)HPLC

• P: 600 and 1000 bar !

5

STM = Sub-2um SP = Superficially PorousTP = Totally Porous

Page 6: Agilent 1290 Infinity LC - Chemical Analysis, Life … 1290 Infinity LC Power Range ¾All analytical columns ¾Method Transfer 1200 bar ¾Higher Resolution ¾Faster Analysis ¾Higher

Agilent 1290 Infinity LCPower Range

All analytical columns

Method Transfer

1200

bar

Higher Resolution

Faster Analysis

Higher (MS) Sensitivity

1000WatersAcquity

Agilent 1290 Infinity LC More viscous solvents

Lower temperatures

600

800Acquity

Dionex RSLC

400

Agilent RRLCThermoAccela

Shimadzu UFLCXR

0

200

Standard LC

6

0 1 2 3 4 5 ml/min

STM = Sub-2um SP = Superficially PorousTP = Totally Porous

Page 7: Agilent 1290 Infinity LC - Chemical Analysis, Life … 1290 Infinity LC Power Range ¾All analytical columns ¾Method Transfer 1200 bar ¾Higher Resolution ¾Faster Analysis ¾Higher

Agilent 1290 Infinity LC Power Range and 4.6mm, 1.8um TP Columns 50 x 4.6 mmg ,

1200

bar

100 x 4.6 mm150 x 4.6 mm250 x 4.6 mm

1000H2O/ACN, 40 °C

600

800

400

0

200

7

00 1 2 3 4 5 ml/min

STM = Sub-2um SP = Superficially PorousTP = Totally Porous

Page 8: Agilent 1290 Infinity LC - Chemical Analysis, Life … 1290 Infinity LC Power Range ¾All analytical columns ¾Method Transfer 1200 bar ¾Higher Resolution ¾Faster Analysis ¾Higher

Agilent 1290 Infinity LC Power Range and 2.1mm, 1.8um TP Columns

50 x 2.1 mm100 x 2.1 mm

1200

bar

150 x 2.1 mm250 x 2.1 mm

0.45 mL/min

H O/ACN 40 °C

1000

H2O/ACN, 40 C

600

800

400

600

ZORBAX RRHT SB-C182.1 x 50mmH2O/ACN2 4 l/ i

0

2002.4ml/minT=95 °C560bar

8

00 1 2 3 4 5 ml/min

STM = Sub-2um SP = Superficially PorousTP = Totally Porous

Page 9: Agilent 1290 Infinity LC - Chemical Analysis, Life … 1290 Infinity LC Power Range ¾All analytical columns ¾Method Transfer 1200 bar ¾Higher Resolution ¾Faster Analysis ¾Higher

How to achieve highest Peak Capacity at given Gradient Time and System Pressure?

• t_g < 5min: 50 mm Column Length

By applying maximum Flow Rate (and Temperature)!

• 5min < t_g < 30min: 100 mm Column Length

• t_g > 30min: 150mm Column Length

P Petersson et al (AZ) J Sep Sci D Guillarme et al Journal of Chromatography

June 06, 2005Page 9

P. Petersson et al (AZ), J.Sep.Sci, 31, 2346-2357, 2008

D. Guillarme et al, Journal of Chromatography A, 1216 (2009) 3232–3243, 2009

Page 10: Agilent 1290 Infinity LC - Chemical Analysis, Life … 1290 Infinity LC Power Range ¾All analytical columns ¾Method Transfer 1200 bar ¾Higher Resolution ¾Faster Analysis ¾Higher

Critical Comparison of TP 1.7um and SP 2.7um Particle Columns under Optimized Ultra-high Pressure Conditions. Xiaoli Wang et al, AstraZeneca, High-Speed LC Symposium, Pittcon 2009

Theory Experiment

Wed, LH5, 11:30am

Page 11: Agilent 1290 Infinity LC - Chemical Analysis, Life … 1290 Infinity LC Power Range ¾All analytical columns ¾Method Transfer 1200 bar ¾Higher Resolution ¾Faster Analysis ¾Higher

Agilent 1290 Infinity LC Power Range and 2.7um SP Columns

50 x 4.6 mm100 x 4.6 mm150 x 4 6 mm

1200

g

bar

150 x 4.6 mm250 x 4.6 mm150 x 3 mm

1000

250 x 3 mm150 x 2.1

H2O/ACN, 40 °C

600

800 2.1x50, T=40C

400

6002.1x50, T=80C

0

200

00 1 2 3 4 5

11

ml/min

STM = Sub-2um SP = Superficially PorousTP = Totally Porous

Page 12: Agilent 1290 Infinity LC - Chemical Analysis, Life … 1290 Infinity LC Power Range ¾All analytical columns ¾Method Transfer 1200 bar ¾Higher Resolution ¾Faster Analysis ¾Higher

Agilent 1290 Infinity LC- Resolution per Time: Superficially Porous Columnsp p y

Peak Capacity* ~ 170 in 24 sec.World Record “ *Pc = based on 2nd peak W(1/2)

„World Record.

0.095sec PW

0.140secPW

Tranquilizer Sample

Column: Superficially Porous

50 x 2.1 mm, 2.7 µm, µ

F = 3.5 ml/min, P = 700 bar

Gradient 2-92 % ACN in 0.4 min

T= 80 °CT= 80 °C

12

Page 13: Agilent 1290 Infinity LC - Chemical Analysis, Life … 1290 Infinity LC Power Range ¾All analytical columns ¾Method Transfer 1200 bar ¾Higher Resolution ¾Faster Analysis ¾Higher

1290 Infinity LC and 6460 QQQ Jet Stream- Resolution per Time: Totally Porous Columns

5x10

4

6

8

+ MRM (455.29999 -> 165.09961) std-t0-1500ulmin-column3-60C-r003.d

1

Metabolic Stability Assay• Column: ZORBAX RRHD SB-C18

VerapamilT = 0.54 min

Avg W1/2 = 0.44 sec 11 points across WRel. Area RSD [%] = 4.0

p y

2

5x10

2

3

+ MRM (386.00000 -> 122.09961) std-t0-1500ulmin-column3-60C-r003.d

1

• Column: ZORBAX RRHD SB-C18 • Dimension: 2.1x50, 1.8um

• T = 60°CBuspironeAvg W1/2 = 0.70 sec 20 points across W

1

2

4x10

2

+ MRM (272.20001 -> 215.19922) std-t0-1500ulmin-column3-60C-r003.d

1

• F = 1.5 mL/min• P = 1070 bar

PW = 0 37 0 98 sec

pT = 0.22 min

pRel. Area RSD [%] = 4.2

Avg W = 0 98 sec

0.5

1

1.5

2

3x10- MRM (294.00000 -> 250.00000) std-t0-1500ulmin-column3-60C-r003.d

1

• PW = 0.37 – 0.98 sec114 msec CT, 6 Transitions, 30ms polarity switching

• 9 – 28 Data Points across Peak• Area RSD = 2.2 – 7.8%

DextromethorphanT = 0.30 min

Avg W1/2 = 0.98 sec 28 points across WRel. Area RSD [%] = 2.2

1

2

3

41

0 1 0 2 0 3 0 4 0 5 0 6 0 7 0 8 0 9 1 1 1 1 2

Area RSD 2.2 7.8%

• Analysis time < 0.8 min• Run time = 1.5 min

DiclofenacT = 0.73 min

Avg W1/2 = 0.37 sec 9 points across WRel. Area RSD [%] = 7.8

Counts vs. Acquisition Time (min)0.1 0.2 0.3 0.4 0.5 0.6 0.7 0.8 0.9 1 1.1 1.2

Page 14: Agilent 1290 Infinity LC - Chemical Analysis, Life … 1290 Infinity LC Power Range ¾All analytical columns ¾Method Transfer 1200 bar ¾Higher Resolution ¾Faster Analysis ¾Higher

Pharma Impurity Analysis: HPLC to UHPLC- Highest Speed with Highest Sensitivity & Precisiong p g y

150 x 3.0 mm, 3.5 µmMethod Successfully Validated• Metoclopramide PN 5990-3981EN

14 min

Metoclopramide, PN 5990-3981EN, . G. Vanhoenacker, F. David, P. Sandra

• LOD: 0.001 - 0.005 % w/w relative to main compound (0 5 2 5ng on column)

100 x 2 1 mm 1 7 µm

main compound (0.5-2.5ng on-column) 10 - 50x more sensitive than required

• RSD 0.05% Impurities: 0.5 - 2.7%100 x 2.1 mm, 1.7 µmPmax = 880 bar

RSD 0.005% Impurities: 1.4 - 4.5%

3x / 2x more precise than required

3 min

14

Page 15: Agilent 1290 Infinity LC - Chemical Analysis, Life … 1290 Infinity LC Power Range ¾All analytical columns ¾Method Transfer 1200 bar ¾Higher Resolution ¾Faster Analysis ¾Higher

1290 Infinity LC with 1200bar ZORBAX RRHD– Resolution: Totally Porous Columns

Rs3,2: 4.92N6 : 29600P 560 b

20:80 H2O:ACN, F = 0.5 mL/min, T = ambient

150 mmmAU

200

250

300

P: 560 barα3,2: 1.15Pw2: 0.015 minPw6: 0.023 min50

100

150

200

Rs (3,2): 6.75N6 49200

150 +100 mm = 250mm1. Uracil2 A t h

min0 0.5 1 1.5 2 2.5 3 3.5

0

mAU

250

300

N6 : 49200P: 1000 barα3,2: 1.15Pw2: 0.017 min

2. Acetophenone3. Propiophenone4. Butyrophenone5. Valerophenone6. Hexanophenone

2

6

543

100

150

200

250

Pw6: 0.029 min11

min0 0.5 1 1.5 2 2.5 3 3.5

0

50

N = 50 000 in less than 3 minutes

Group/Presentation TitleAgilent Restricted

Month ##, 200XPage 15

N = 50,000 in less than 3 minutes

Page 16: Agilent 1290 Infinity LC - Chemical Analysis, Life … 1290 Infinity LC Power Range ¾All analytical columns ¾Method Transfer 1200 bar ¾Higher Resolution ¾Faster Analysis ¾Higher

1290 Infinity LC + 6460 QQQ Jet Stream- Analysis of 300 Pesticides in 15 min.

600 Dynamic MRM Transitions9 replicates analyzed at 200 pg level

Column: 2.1 x 150mm, 1.7umMobile phase: A = H2O w/5 mM ammonium formate + 0.01% formic

B = MeOH w/5 mM ammonium formate + 0.01% formicLC Gradient: 8%B (T=0) to 92%B (T=15 min), hold 3 min

40 Pesticides in 1 minPW ~2 sec FWHM

p y pgMean area %RSD = 3.2 Mean height %RSD= 3.6

( ) ( ),Column Temperature: 70°CLC flow rate: 0.5 ml/min

Agilent MassHunter Workstation

February-2009Page 16

Page 17: Agilent 1290 Infinity LC - Chemical Analysis, Life … 1290 Infinity LC Power Range ¾All analytical columns ¾Method Transfer 1200 bar ¾Higher Resolution ¾Faster Analysis ¾Higher

Agilent 1290 Infinity LC”Next Generation Technology”

for highest Flexibility and Performance

• New! 1290 Infinity Binary Pump

• New! 1290 Infinity Autosampler

• New! 1290 Infinity FlexCubeNew! 1290 Infinity FlexCube

• New! 1290 Infinity Column Compartment

N ! 1290 I fi it Di d A D t kt• New! 1290 Infinity Diode-Array Detektor

• New! ZORBAX RRHD Columns

17

Page 18: Agilent 1290 Infinity LC - Chemical Analysis, Life … 1290 Infinity LC Power Range ¾All analytical columns ¾Method Transfer 1200 bar ¾Higher Resolution ¾Faster Analysis ¾Higher

1290 Infinity LC Binary Pump1290 Infinity LC Binary Pump1290 Infinity LC Binary Pump1290 Infinity LC Binary Pump

Page 18

Page 19: Agilent 1290 Infinity LC - Chemical Analysis, Life … 1290 Infinity LC Power Range ¾All analytical columns ¾Method Transfer 1200 bar ¾Higher Resolution ¾Faster Analysis ¾Higher

1290 Infinity LC Binary Pump-The Next Generation in LC Pump Technology

Highest Speed and Resolution1200 bar @ 2.0 mL/min

1000bar @ 3.5ml/min

800 bar @ 5.0 mL/min

Lowest Delay Volume: 10 µl / 45 µly

Highest Precision and SensitivityLowest „Mixing Noise“

Lowest RT RSDLowest RT RSD

Longest Maintenance Intervalls Automatic Leak-Correction

150 Lit S l t150 Liter Solvent

Convenient OperationAutomatic „Purge“

Page 19

Page 20: Agilent 1290 Infinity LC - Chemical Analysis, Life … 1290 Infinity LC Power Range ¾All analytical columns ¾Method Transfer 1200 bar ¾Higher Resolution ¾Faster Analysis ¾Higher

1290 Infinity LC Binary Pump Technology- Maximum Power and Robustness

Page 20

Page 21: Agilent 1290 Infinity LC - Chemical Analysis, Life … 1290 Infinity LC Power Range ¾All analytical columns ¾Method Transfer 1200 bar ¾Higher Resolution ¾Faster Analysis ¾Higher

1290 Infinity LC Binary PumpHeat Dissipation and Robustnessp

New Piston Material: SSiC (Sintered Silicon Carbide)SSiC (Sintered Silicon Carbide)

Optimized heat dissipation enables • higher pressure x flow rates (1200bar at 2 ml/min)• longer seal lifetime / maintenance intervalls (>150L)• longer seal lifetime / maintenance intervalls (>150L)• one seal for reversed and normal phase

Multi-layer capillary for optimized heat exchange between pump heads

Page 21

heat exchange between pump heads

Page 22: Agilent 1290 Infinity LC - Chemical Analysis, Life … 1290 Infinity LC Power Range ¾All analytical columns ¾Method Transfer 1200 bar ¾Higher Resolution ¾Faster Analysis ¾Higher

1290 Infinity Binary Pump- Lowest Delay Volume and Lowest „Mixing Noise“

1200 Series Channel A

Passive Damper0 - 300 µl

Passive Mixer400 µl

1200 SeriesChannel B Very Low Noise

~ 800 µl

1290 InifinityChanel A

1290 InifinityChanel B

Lowest Noise

+ =Aktive Damping10µl

Jet Weaver Mixer35µl

• min. 50% lower Delay Volume• 10µl / 45µl• 80x / 20x lower

Page 22

• 80x / 20x lower

Page 23: Agilent 1290 Infinity LC - Chemical Analysis, Life … 1290 Infinity LC Power Range ¾All analytical columns ¾Method Transfer 1200 bar ¾Higher Resolution ¾Faster Analysis ¾Higher

Agilent Jet Weaver- Next Generation highest-efficient Mixingg g

Highest Mixing Efficiency at lowest Volume

• 35 µl for standard UV Applications

• 100 µl for TFA Applications

Technology:• Proprietary multi-layer, microfluidics Technology• Diffusion bonded stainless steel, edged structures

(100x120um)

23

Page 24: Agilent 1290 Infinity LC - Chemical Analysis, Life … 1290 Infinity LC Power Range ¾All analytical columns ¾Method Transfer 1200 bar ¾Higher Resolution ¾Faster Analysis ¾Higher

Active Damping- The Technology and its Advantagesgy g

Active Dampingp g

• Lowest noise at lowest delay volume (10µl/45ul) for highest UV sensitivity

FirmwarePump

Intelligence

Full characterisation of solvent properties

Tuning (realtime optimization basedon static and dynamic parameters) for highest UV sensitivity

in ultra-fast runs.

• Lowest RT RSD for most precise peak ID

on static and dynamic parameters)

+

precise peak ID

• Automatic compensation of small leaks for longer maintenance intervalls

Dedicated µ-processor for realtime piston controlHighest resolution pump drives:65000 steps, 300 pL/step, 50x better

HardwareSmooth motion

control maintenance intervalls and lower RT RSDIndependent piston drives

control

Page 24

Page 25: Agilent 1290 Infinity LC - Chemical Analysis, Life … 1290 Infinity LC Power Range ¾All analytical columns ¾Method Transfer 1200 bar ¾Higher Resolution ¾Faster Analysis ¾Higher

1290 Infinity LC – UFLC Retention Time Precision

RT RSD = 0.005% !„World Record.“

P i i P k 1 P k 2 P k 3 P k 3 P k 5 P k 6 P k 7 P k 8 P k 9Precision Peak 1 Peak 2 Peak 3 Peak 3 Peak 5 Peak 6 Peak 7 Peak 8 Peak 9

% RSD RT 0.016 0.0077 0.0052 0.0058 0.0058 0.0054 0.0059 0.0057 0.0080

% RSD Area 0 40 0 39 0 43 0 47 0 40 0 46 0 44 0 52 0 56Phenone Standard, 10 Consecutive Runs0.5 µl 0.40 0.39 0.43 0.47 0.40 0.46 0.44 0.52 0.56

2.1 x 50mm RRHD SB C18, 1.8µm1.2ml/min, 0.5ul, 40C

80Hz, 245/10nm, REF 360/80nm5 - 95% ACN in 0.5min

Page 25

Page 26: Agilent 1290 Infinity LC - Chemical Analysis, Life … 1290 Infinity LC Power Range ¾All analytical columns ¾Method Transfer 1200 bar ¾Higher Resolution ¾Faster Analysis ¾Higher

1290 I fi it Di d A D t kt1290 I fi it Di d A D t kt1290 Infinity Dioden-Array Detektor1290 Infinity Dioden-Array Detektor

June 06, 2005Page 26

Page 27: Agilent 1290 Infinity LC - Chemical Analysis, Life … 1290 Infinity LC Power Range ¾All analytical columns ¾Method Transfer 1200 bar ¾Higher Resolution ¾Faster Analysis ¾Higher

1290 Infinity Diode-Array Detector- The Next Generation in UV-Vis Detection

GratingMirror

Programmableslit

Deuterium lamp

slit

Deuterium lamp

1024 elementdiode-array

Max-Light Cartridge CellNext Generation Flow Cell Design

Non-Coated Fused Silica Optofluidic Waveguides

Page 27

Page 28: Agilent 1290 Infinity LC - Chemical Analysis, Life … 1290 Infinity LC Power Range ¾All analytical columns ¾Method Transfer 1200 bar ¾Higher Resolution ¾Faster Analysis ¾Higher

1290 Infinity Diode Array Detector- highest sensitivity under any condition

1200 RRLC (no mixer, damper)5-95% ACN, 0.5 ml/min 80C, 40 Hz, 230nm/360nm

1290 Infinity DADNext Level Sensitivity and Productivity

More automated integration at any condition

1200 DAD SL

More automated integration at any condition2 – 20x higher sens with 1 cm Cell8 – 80x higher sens with 6 cm UHS Cell

Page 28

Page 29: Agilent 1290 Infinity LC - Chemical Analysis, Life … 1290 Infinity LC Power Range ¾All analytical columns ¾Method Transfer 1200 bar ¾Higher Resolution ¾Faster Analysis ¾Higher

1290 Infinity Diode Array Detector- Ultra Sensitivity by 6cm Flow Cell

mAU

DAD1 B, DAD1A, DAD: Signal A, 251 nm/Bw:10 nm Ref 360 nm/Bw:100 nm (F:\AE4-ANTHRACENE\ANTHRA-STD-CEL7.D)DAD1 B, DAD1A, DAD: Signal A, 251 nm/Bw:10 nm Ref 360 nm/Bw:100 nm (F:\AE4-ANTHRACENE\ANTHRA-HS-CEL07.D)

Anthracene, 16.4pg/µlW t /ACN 30/70

35

40

Water/ACN = 30/700.5ml/min, 1µl injection36C, 251nm, PW>0.05min

4 x Higher Sensitivity

20

25

30

High-Sensitivity Standard Cell (1cm)

Ultra High-Sensitivity Cell (6cm)S/N: 2339

10

15

High Sensitivity Standard Cell (1cm)S/N: 612

min0 0.2 0.4 0.6 0.8 1 1.2 1.4 1.6 1.8

0

5

Page 29

Page 30: Agilent 1290 Infinity LC - Chemical Analysis, Life … 1290 Infinity LC Power Range ¾All analytical columns ¾Method Transfer 1200 bar ¾Higher Resolution ¾Faster Analysis ¾Higher

1290 Infinity Autosampler1290 I fi it Fl C b

1290 Infinity Autosampler1290 I fi it Fl C b1290 Infinity FlexCube1290 Infinity FlexCube

Page 30

Page 31: Agilent 1290 Infinity LC - Chemical Analysis, Life … 1290 Infinity LC Power Range ¾All analytical columns ¾Method Transfer 1200 bar ¾Higher Resolution ¾Faster Analysis ¾Higher

1290 Infinity Autosampler- Next Generation Variable Loop Injector

AdvantagesLower volume (80ul)

Highest precision w/o loop change from 0.1 – 40ul

No loss of sample

Lowest Carry Over

<0.004% Chlorhexidine

New Cartridge Loop

Page 31

g p

Page 32: Agilent 1290 Infinity LC - Chemical Analysis, Life … 1290 Infinity LC Power Range ¾All analytical columns ¾Method Transfer 1200 bar ¾Higher Resolution ¾Faster Analysis ¾Higher

1290 Infinity FlexCube- Extending the capabilities g p

Syringe PumpRail for

Valve option 1 Valve option 2Low pressureValve

external Valve

Applications:a) Automated needle seat back-flushing for lower carry overb) Fixed loop injection mode for fastest cycle timesc) and more ...........

Page 32

Page 33: Agilent 1290 Infinity LC - Chemical Analysis, Life … 1290 Infinity LC Power Range ¾All analytical columns ¾Method Transfer 1200 bar ¾Higher Resolution ¾Faster Analysis ¾Higher

1290 Infinity Autosampler plus FlexCube- Fixed loop injection modep j

AdvantagesLow Delay volume (10 µl)y ( µ )

Overlapped injection with lowest carry over

Page 33

Page 34: Agilent 1290 Infinity LC - Chemical Analysis, Life … 1290 Infinity LC Power Range ¾All analytical columns ¾Method Transfer 1200 bar ¾Higher Resolution ¾Faster Analysis ¾Higher

1290 Infinity LC System Delay Volume- Configure the sytem to your needs

Configuration Delay Vol.* / µl

1290 P 101290 Pump 10

1290 Pump + Fixed Loop1 (for MS) 20

1290 Pump + Jet Weaver + Fixed1 Loop (for UV) 551290 Pump Jet Weaver Fixed Loop (for UV) 55

1290 Pump + Variable Loop (for MS) 90

Vendor A1 90

1290 Pump + Jet Weaver + Variable Loop (for UV) 120

RRLC Pump + Variable Loop (for MS) 260

RRLC Pump + Damper/Mixer + Variable Loop (for UV) 740-940

* Delay Volume: 10 % step gradient Definition preliminary data Delay Volume: 10 % step gradient Definition, preliminary data1 5 µl Loop

Page 34

Page 35: Agilent 1290 Infinity LC - Chemical Analysis, Life … 1290 Infinity LC Power Range ¾All analytical columns ¾Method Transfer 1200 bar ¾Higher Resolution ¾Faster Analysis ¾Higher

1290 Infinity1290 Infinity1290 InfinityThermostatted Column Compartment1290 InfinityThermostatted Column Compartment

Page 35

Page 36: Agilent 1290 Infinity LC - Chemical Analysis, Life … 1290 Infinity LC Power Range ¾All analytical columns ¾Method Transfer 1200 bar ¾Higher Resolution ¾Faster Analysis ¾Higher

1290 Infinity Therm. Column Compartment- Next level of Versatility, Automation and PerformanceNext level of Versatility, Automation and Performance

• New generic valve drive • New quick change valve heads (2/6 2/10 9/8 )• New quick-change valve heads (2/6, 2/10, 9/8, ...)

with RFID tags (type, pressure, switches)• Two independent temperatures zones with

improved thermal insulationimproved thermal insulation• New Door Open Sensor• New Capillary Guides and Leak Funnel• Improved Low Dispersion Heat Exchangers• Improved Low Dispersion Heat Exchangers

Page 37: Agilent 1290 Infinity LC - Chemical Analysis, Life … 1290 Infinity LC Power Range ¾All analytical columns ¾Method Transfer 1200 bar ¾Higher Resolution ¾Faster Analysis ¾Higher

1290 Infinity LC Solutions- Flexible Automation

Method Development and Multi-Method• Up to 3 clustered Compartments for up

to 8 Columns 26 Solvents 6 T zonesto 8 Columns, 26 Solvents, 6 T-zones• MD: ChromSword and AutoChrom• Method-Screening: Scouting Wizard • Multi-Method: ChemStation, EZAccess

Alternating Column Regeneration• 2000 Samples/day with overlapped

injections and offline data analysis

Online Sample Preparation• Enrichment• Stripping

37

Stripping

Page 38: Agilent 1290 Infinity LC - Chemical Analysis, Life … 1290 Infinity LC Power Range ¾All analytical columns ¾Method Transfer 1200 bar ¾Higher Resolution ¾Faster Analysis ¾Higher

Gradient Behaviour and Method Transfer – Emulating any (U)HPLC System

Scatter Plot

100

UV Signal

80

90

100

Method Transfer

50

60

70

Same RT of all Peaks

Same Resolution all Peak Pairs

20

30

40

1290 Infinity LC1200 RRLC (damper + mixer)

0 0.2 0.4 0.6 0.8 1 1.2 1.4 1.6 1.8 2

0

10 1290 Infinity LC (+ damper + mixer + capillary)

Delay Volume (ml)

Page 38

Page 39: Agilent 1290 Infinity LC - Chemical Analysis, Life … 1290 Infinity LC Power Range ¾All analytical columns ¾Method Transfer 1200 bar ¾Higher Resolution ¾Faster Analysis ¾Higher

Agilent 1290 Infinity LC- Meeting the Development Objectives- Meeting the Development Objectives

Requirements The Debate is Overq

1. Power Range:encloses any (U)HPLC

• Methods: transferable !

• ID: 4 6 3 and 2 1 mm !2. Delay Volume:

lowest, adjustable

3. Sensitivity, Precision:

• ID: 4.6, 3 and 2.1 mm !

• Size: STM and larger !

• Particle: TP and SP !y,better than any (U)HPLC

4. Injection Principle:variable and fixed loop

Particle: TP and SP !

• Flow: low and high !

• T: low and high !p

5. Gradient Behaviour:emulate any (U)HPLC

g

• P: 600, 1000 bar and higher!

39

STM = Sub-2um SP = Superficially PorousTP = Totally Porous

Page 40: Agilent 1290 Infinity LC - Chemical Analysis, Life … 1290 Infinity LC Power Range ¾All analytical columns ¾Method Transfer 1200 bar ¾Higher Resolution ¾Faster Analysis ¾Higher

Agilent 1290 Infinity LC

More Powerful

Resolution and Speed: 1200 bar (2 ml/min)800 bar (5 ml/min)20 µl Delay Volume

1200bar RRHD Columns N 50 000 in 3min

Sensitivity UV : 2 – 20x with 10 mm Cell

1200bar RRHD Columns N = 50,000 in 3minRT Precision: 0.005% RSD (1min run)

8 – 80x with 60 mm CellCondition independent

Sensitivity MS/MS: < 1 fg with Jet StreamCarry Over: < 0 004% Chlorhexidine

More Sensitive

Carry Over: < 0.004% Chlorhexidine

Supports all analytical columns Enables Method transfer from/to any (U)HPLC

MoreFlexible

Flexible Injection: flow-through and fixed loopHighest-Throughput Solution (> 2000 samples/day)Method Development and Multi-method Solutions: up to 8 columns 26 solvents 6 temperature zones

40

up to 8 columns, 26 solvents, 6 temperature zones

Page 41: Agilent 1290 Infinity LC - Chemical Analysis, Life … 1290 Infinity LC Power Range ¾All analytical columns ¾Method Transfer 1200 bar ¾Higher Resolution ¾Faster Analysis ¾Higher

Agilent 1290 Infinity LCg y

June 06, 2005Page 41