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ACQUITY UPLC TUnAbLe UV DeTeCTor
ACQUITY UPLC Tunable UV Detector
The Waters® ACQUITY UPLC™ Tunable UV (TUV) Detector is a dual-wavelength, ultraviolet/visible detector specifically designed for use in the ACQUITY UPLC System. Optimized for Ultra Performance LC™, the TUV detector’s innovative light-guided flow cell, low-noise electronics and support for data rates up to 80 Hz result in a detector that delivers the utmost in UPLC™ sensitivity.
The TUV detector offers:
• ACQUITY UPLC light-guiding flow cells
• Fast data rates optimized for UPLC separations
• Unsurpassed sensitivity and linearity for the quantitation of low-level impurities
• Configurable and intuitive instrument control software
Waters ACQUITY UPLC System with the ACQUITY UPLC TUV Detector.
optimized for UPLC separations
Waters ACQUITY UPLC System has been shown to significantly reduce run times by up to ten percent. The ACQUITY UPLC TUV detector, coupled with our innovative ACQUITY UPLC column chemistries and separations modules, leverages ultra-low dwell volumes and low-noise, high-speed detection to bring you productivity, sensitivity and resolution that are unprecedented in HPLC techniques today.
Additionally, the TUV Detector may be interfaced directly in line with mass spectrometers. The ACQUITY UPLC system’s low dispersion fluidics eliminate the need to remove the optical detector or to split the flow path between the two detectors, in order to maintain optimal resolution.
Light-Guided Flow Cell Technology
Small bore high-capacity columns, as used in ACQUITY UPLC, produce small volume peaks. To avoid bandpreading and maintain concentration, the detector’s flow cell volume must be correspondingly small. Additionally, high light levels are needed to preserve a high signal-to-noise ratio.
The ACQUITY UPLC TUV Detector’s uniquely designed light-guided flow cell was created with optimum path length and high light throughput in mind. The cell uses Teflon AF, an internally reflective surface, to improve light transmission efficiency by eliminating internal absorption. This design complements and maintains the significant advantages gained by the use of the ACQUITY UPLC fluidic technology.
Light rays entering the liquid core of the flow cell are internally reflected when they meet the Teflon AF boundary. These rays are transmitted through the cell without loss, except for absorption by the sample.
Speed
High-throughput laboratories have a constant need to generate more information and process more samples, while maintaining the quality of the data. In today’s laboratories, Ultra Performance LC systems can generate peaks less than 0.5 seconds wide and analysis times as little as half a minute long.
The ACQUITY UPLC TUV Detector has been holistically designed with the ACQUTY UPLC System to offer a wide variety of sampling rates, from 1 to 80 Hz, that fit the requirements of any ultra-fast, ultra performance application, while still conforming to the foundations of sensitivity and resolution. Effective integration algorithms for ultra-fast UPLC separations, as well as independent optimization of time constants, allow for accurate and reproducible integration.
1 Point per Second2 Points per Second5 Points per Second
10 Points per Second20 Points per Second40 Points per Second80 Points per Second
0.00
0.05
0.10
0.15
0.20
0.25
0.30
0.35
0.40
0.45
0.50
Minutes0.12 0.14 0.16 0.18 0.20 0.22 0.24 0.26 0.28 0.30 0.32 0.34 0.36 0.38 0.40 0.42 0.44 0.46 0.48
Detector data acquisition rates must be fast enough to enable detection of narrow UPLC peaks. Accurate integration at slower rates would be impossible.
1
2
3
45
6
78
9
10
11
12
13
C-2
2R (
epim
er B
)
C-2
2S (
epim
er A
)
16 1718
Minutes
AU
0.000
0.001
0.002
0.003
0.004
0.005
0.006
0.007
0.008
0.009
0.010
0.50 1.00 1.50 2.00 2.50 3.00 3.50 4.00 4.50 5.00 5.50 6.00 6.50 7.00 7.50 8.00
0.02411Impurity 18
0.03920Impurity 17
0.02714Impurity 16
48.50026341C-22S
49.00029169C-22R
1.6301089Impurity 13
0.200140Impurity 12
0.05643Impurity 11
0.02215Impurity 10
0.15089Impurity 9
0.01816Impurity 8
0.02528Impurity 7
0.04651Impurity 6
0.02430Impurity 5
0.01010Impurity 4
0.03269Impurity 3
0.01138Impurity 2
0.033120Impurity 1
Area %S/NCompound
} 97.5
Sensitivity
Over the years, the pursuit of increased sensitivity has been driven by the need to detect compounds at increasingly lower levels. The ACQUITY UPLC TUV Detector has been designed, from the ground up, to provide the highest performance in UV/Visible detection for UPLC applications.
The ACQUITY UPLC TUV detector offers both the superior sensitivity and the linear dynamic range needed for the detection of even the lowest-level impurities allowing for the simultaneous quantitation of major and minor compounds in both routine and complex, ultra-fast UPLC separations.
-0.0010
-0.0009
-0.0008
-0.0007
-0.0006
-0.0005
-0.0004
-0.0003
-0.0002
-0.0001
Minutes
1.80 1.90 2.00 2.10 2.20 2.30 2.40 2.50 2.60 2.70 2.80 2.90
AU
2.20.00342.714Impurity 5
5.80.01062.549Impurity 4
2.60.00372.504Impurity 3
6454499.9542.263Butamben
16.40.02292.231Impurity 2
4.10.00542.174Impurity 1
Signal-to-NoiseArea %Retention Time(Minutes)Compound
Impu
rity
2 -
2.23
1
Impu
rity
3 -
2.50
4
Impu
rity
4 -
2.54
9
Impu
rity
5 -
2.71
4
But
ambe
n -
2.26
3
Impu
rity
1 -
2.17
4
Even at trace impurity levels as low as .003%, the ACQUITY UPLC TUV is the benchmark in sensitivity.
Linearity
The ACQUITY UPLC TUV has a linear dynamic range that extends more than 3.5 orders of magnitude allowing for the identification and quantitation of both low level impurities (< 0.01%) and their parent compounds over a wide concentration range.
Additionally, the unique design of ACQUITY UPLC light-guided flow cells delivers the same level of quality data regardless of flow cell volume and pathlength.
% Absorber in Eluent
Ab
sorb
ance
(A
U)
TUV - Analytical Flow CellTUV - High Sensitivity Flow Cell
2.2% Deviation at 2.5 AU2.4% Deviation at 2.5 AU
5.0% Deviation at 3.0 AU5.0% Deviation at 3.0 AU
0
0.5
1
1.5
2
2.5
3
3.5
0 10 20 30 40 50 60 70 80
The TUV’s low dispersion characteristics and superior noise behavior means no compromise in detection performance.
Waters, ACQUITY UPLC, Empower, MassLynx, UPLC and Ultra Performance LC are trademarks of Waters Corporation. All other trademarks are property of their respective owners. ©2006 Waters Corporation Printed in the USA March 2006 720001333EN JH-CV
Console and Software
The ACQUITY UPLC TUV Detector is equipped with a customizable instrument console, for both Empower™ and MassLynx™ software that enables users to easily stay in stay control of the detector. Interactions that were historically done through an instrument keypad—instrument setup, status monitoring, and diagnostics—are now easily accessible through an intuitive, easy-to-learn software interface.
The console uses a simple navigational approach, combined with instrument default parameters, defined range settings, tool tips, and online help, that makes system navigation and instrument usability a snap. Furthermore, at any time the control panel can display the operating status of the module providing assurance that the system is working properly.
The console of the ACQUITY UPLC TUV gives users accessibility to all primary detector functions through a single, intuitive software interface.
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WATERS CORPORATION 34 Maple St. Milford, MA 01757 U.S.A. T: 1 508 478 2000 F: 1 508 872 1990 www.waters.com