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PATENT ABSTFlACTS 291 5989388 ANTIBODIES FOR SALICYLATE AND THEIR PREPARATION Prithipa Singh, Michael Prisbylla assigned to Syntex (U S A ) Inc Conjugates of salicylic acid and certain poly- (amino acids), which are either antigenic or en- zymes, are provided. Antibodies raised against the antigenic poly(amino acids) and the enzyme conjugates are used as reagents in immuno- assays. 5089392 FLUOROGENIC SUBSTRATES FOR MEASUREMENT OF LYSOSOMAL ENZYME ACTIVITIES WITHIN INTACT CELLS Stephen P F Miller, Roscoe Brady assigned to The United States of America as represented by of the Department of Health and Human Ser- vices Fluorogenic substrates and compositions con- taining the same are useful for detecting en- zymatic activity within intact cells. The fluorogenic substrates are lysosomotropic derivatives of 2,3-dicyano-hydroquinone (DCH). These derivatives may be employed for detecting lysosomal enzymatic activity within in- tact cells using fluorescent microscopy and cyto- metry. 5089395 VIABLE MICROORGANISM DETECTION BY INDUCED FLUORESCENCE A Peter Snyder, David B Greenberg, Pasquale V Scarpino assigned to University of Cincinnati The present invention concerns a spectrometric technique to determine microorganism detection and identification by taking advantage of the in- herent extracellular enzymes present in living or- ganisms, as opposed to dead, non-enzyme producing organisms. These enzymes are har- nessed in the in vivo reactions with a non- fluorescent dye containing a select organic functional group that is known to be cleaved or hydrolyzed by the certain enzyme. The dye is tailored such that one of the products fluoresces, so that by employing a conventional spectro- fluorimeter, the rate of fluorescence can be deter- mined. By subjecting a plurality of samples having different cellular concentrations of viable microorganisms to the same non-fluorescent dye, or by subjecting the same bacterial sample to a number of different non-fluorescent dyes, a pattern of fluorescent rates emerge. By em- ploying the pattern recognition set to standard microorganism fluorescent response curves, microorganism detection and identification can be determined. Thus, the present invention con- cerns a process for determining microorganism detection. identification and concentration. 5089413 METHOD AND APPARATUS FOR CULTURING WITH MICROBIOLOGICAL DRY CULTURE MEDIUM Robert L Nelson, Paul E L Hansen assigned to Minnesota Mining and Manufacturing Com- pany A device useful for growing aerobic micro- organisms, particularly molds. The device em- ploys a relatively small amount of water- reconstitutable medium that can be sealed in order to prevent desiccation and contamination of the medium yet still provide an adequate supply of air to the medium, by the use of a mem- brane underlying the medium, to support the growth of aerobic microorganisms. 5089420 COMPOSITION, DEVICE AND METHOD OF ASSAYING FOR A PEROXIDATIVELY ACTIVE SUBSTANCE UTILIZING AMINE BORATE COMPOUNDS James P Albarella, Michael Pugia assigned to Miles Inc A new and improved test device and method of determining the presence or concentration of a peroxidatively active substance, such as hemoglobin, in a test sample are disclosed. The test device includes a test pad comprising a suitable carrier matrix incorporating an in- dicator reagent composition capable of inter-

5089420 Composition, device and method of assaying for a peroxidatively active substance utilizing amine borate compounds

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Page 1: 5089420 Composition, device and method of assaying for a peroxidatively active substance utilizing amine borate compounds

PATENT ABSTFlACTS 291

5989388

ANTIBODIES FOR SALICYLATE AND THEIR PREPARATION

Prithipa Singh, Michael Prisbylla assigned to Syntex (U S A ) Inc

Conjugates of salicylic acid and certain poly- (amino acids), which are either antigenic or en- zymes, are provided. Antibodies raised against the antigenic poly(amino acids) and the enzyme conjugates are used as reagents in immuno- assays.

5089392

FLUOROGENIC SUBSTRATES FOR MEASUREMENT OF

LYSOSOMAL ENZYME ACTIVITIES WITHIN INTACT

CELLS

Stephen P F Miller, Roscoe Brady assigned to The United States of America as represented by of the Department of Health and Human Ser- vices

Fluorogenic substrates and compositions con- taining the same are useful for detecting en- zymatic activity within intact cells. The fluorogenic substrates are lysosomotropic derivatives of 2,3-dicyano-hydroquinone (DCH). These derivatives may be employed for detecting lysosomal enzymatic activity within in- tact cells using fluorescent microscopy and cyto- metry.

5089395

VIABLE MICROORGANISM DETECTION BY INDUCED

FLUORESCENCE

A Peter Snyder, David B Greenberg, Pasquale V Scarpino assigned to University of Cincinnati

The present invention concerns a spectrometric technique to determine microorganism detection and identification by taking advantage of the in- herent extracellular enzymes present in living or- ganisms, as opposed to dead, non-enzyme producing organisms. These enzymes are har- nessed in the in vivo reactions with a non- fluorescent dye containing a select organic functional group that is known to be cleaved or

hydrolyzed by the certain enzyme. The dye is tailored such that one of the products fluoresces, so that by employing a conventional spectro- fluorimeter, the rate of fluorescence can be deter- mined. By subjecting a plurality of samples having different cellular concentrations of viable microorganisms to the same non-fluorescent dye, or by subjecting the same bacterial sample to a number of different non-fluorescent dyes, a pattern of fluorescent rates emerge. By em- ploying the pattern recognition set to standard microorganism fluorescent response curves, microorganism detection and identification can be determined. Thus, the present invention con- cerns a process for determining microorganism detection. identification and concentration.

5089413

METHOD AND APPARATUS FOR CULTURING WITH

MICROBIOLOGICAL DRY CULTURE MEDIUM

Robert L Nelson, Paul E L Hansen assigned to Minnesota Mining and Manufacturing Com- pany

A device useful for growing aerobic micro- organisms, particularly molds. The device em- ploys a relatively small amount of water- reconstitutable medium that can be sealed in order to prevent desiccation and contamination of the medium yet still provide an adequate supply of air to the medium, by the use of a mem- brane underlying the medium, to support the growth of aerobic microorganisms.

5089420

COMPOSITION, DEVICE AND METHOD OF ASSAYING FOR A

PEROXIDATIVELY ACTIVE SUBSTANCE UTILIZING AMINE

BORATE COMPOUNDS

James P Albarella, Michael Pugia assigned to Miles Inc

A new and improved test device and method of determining the presence or concentration of a peroxidatively active substance, such as hemoglobin, in a test sample are disclosed. The test device includes a test pad comprising a suitable carrier matrix incorporating an in- dicator reagent composition capable of inter-

Page 2: 5089420 Composition, device and method of assaying for a peroxidatively active substance utilizing amine borate compounds

298 PA’ENT ABSTRACTS

acting with a perioxidatively active substance to produce a detectable or measurable response. In addition, a new and improved indicator reagent composition, comprising an indicator dye, such as a redox indicator, like a benzidine indicator; a hydroperoxide; an amine borate compound having the general structural formula: See Pa- tent for Chemical Structure wherein R 1, R2, and R3 are, independently, methyl groups or ethyl groups, and m, n and p are numerals ranging from one to about three; a buffer, is incorporated into a suitable carrier matrix to provide a more accurate and trustworthy assay of a test sample for a peroxidatively active substance. The im- proved method and composition are especially useful in the assay of urine for occult blood.

SO89423

IMMUNOASSAY METHODS AND REAGENTS AND METHODS FOR

PRODUCING THE LATTER

Eleftherios P Diamandis, J Alexander Lowden, Toronto, Canada assigned to CyberFluor Inc

Immunoassay and methods for using fluores- cent chelates of lanthanide metal ions in con- junction with immunoreactive bodies to permit fluorescent assay in liquid or dry samples. The assay reagent comprises a residue of an immuno- reactive body linked to a residue of a protein or polypeptide. The protein or polypeptide is labeled by substitution with a ligand forming a fluorescent chelate with lanthanide metal ion such as europium. The reagent when used in an immunoassay, binds to the immobilized immunoreactive body and excess is washed away. The sample can be dried for later analysis by a suitable fluoremeter.

5089530

NOVEL FERMENTATION PRODUCT WITH ANTIPARASITIC

ACTIVITY

Athanasios Tsipouras, Dan A Ostlind, Otto D Hensens, Deborah L Zink assigned to Merck & co Inc

The fermentation of a fungal organism iden- tified as Chrysosporium meridarium produces a novel binaphthalene compound which is a highly potent antiparasitic, insecticidal, and anthelmin- thic agent.

5089630

DIOXETANES FOR USE IN ASSAYS

Irena Y Bronstein, Brook Edwards, Larry Kricka, John Voyta

A dioxetane that includes a fluorescent chromophore Spiro-bound at the 4-carbon of the dioxetane. The dioxetane has the formula See Patent for Chemical Structure where X is CR7RS,O, S, or N-R (where each R7, R8, and R, independently, is H, alkyl, heteroalkyl, aryl, heteroaryl, cycloalkyl, cycloheteroalkyl, aralkyl, alkaryl, or an enzyme cleavable group), and each Rl, R2, R3, R4, R5, andR6, independently, is H, an electron-withdrawing group, an electron- donating group, heteroaryl, or an enzyme cleavable group, or groups RI-R6 together form a ring, and T is a substituted or unsubstituted aryl, polyaryl, cycloalkylidene or poly- cycloalkylidene group Spiro-bound at the 3- carbon of the dioxetane. The dioxetane can be decomposed by direct cleavage of the dioxetane O-O bond or by cleavage of an enzyme cleavable group bonded to the dioxetane to form a luminescent substance that includes the coumarin portion of the dioxetane. The diox- etanes are used in an assay to detect a member of a specific binding pair or an enzyme.

5091206

PROCESS FOR PRODUCING MAGNETICALLY RESPONSIVE

POLYMER PARTICLES AND APPLICATION THEREOF

Chao-Hue Wang, Dinesh Shah assigned to Bax- ter Diagnostics Inc

This invention provides a novel process of pro- ducing magnetically responsive polymer par- ticles comprising polymeric core particles coated evenly with a layer of polymer containing magnetically responsive metal oxide. A wide variety of polymeric particles with sizes ranging from 1 to 100 microns can be used a core par- ticles and transformed into magnetically respon- sive polymer particles. The surface of these magnetically responsive polymer particles can be coated further with another layer of func- tionalized polymer. These magnetically respon- sive polymer particles can be used for passive or covalent coupling of biological material such as antigens, antibodies, enzymes of DNA/RNA