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PATENT ABSTRACTS 294 5084381 ASSAY METHOD FOR DETECTING HYDROGEN PEROXIDE Kengo Akimoto, Sumio Asami, Yukikaz Saeki, Osaka, Japan assigned to Suntory Limited An improved method of chemiluminescence as- say for hydrogen peroxide wherein a peroxidase of microorganism origin is used in place of hor- seradish peroxidase. This allows an assay of unexpectedly high sensitivity to be achieved. 5084382 AGENT AND PROCESS FOR THE DETECTION OF REDOX SYSTEMS Gunte Frey, Gerd Zimmermann, Ellerstadt, Federal Republic Of Germany assigned to Boehringer Mannheim GmbH The present invention is concerned with the use of aniline derivatives of the general formula: See Patentfor Chemical Structure (I) wherein Rl is a hydrogen atom or a lower alkyl or aryl radical, R2 is a hydrogen atom or a lower alkyl radical which can be substituted by hydroxyl, amino, carboxyl, lower alkoxycarbonyl, lower alkanoylamido, aryl lower alkyl or aryl or by a group of the structure: See Patent for Chemical Strucfure in which R5 and R6 have the meanings given hereinafter, R3 is a hydrogen or halogen atom or a carboxyl group, R4 and R’4, which can be the same or different, are hydrogen or halogen atoms, carboxyl groups or lower alkoxy radicals or lower alkyl radicals which are pre- ferably in the m-position and Rl and R4 or R2 and R4 or R4 and R’4, when the two substituents are ortho to one another, can together form a saturated or unsaturated hydrocarbon chain containing 2 to 6 carbon atoms which can be substituted by hydroxyl or 0x0 groups, R5 is a hydrogen atom or a lower alkyl radical and R6 is a hydroxyl group or a lower alkoxy, lower alkyl or aryl radical; and of the salts thereof with acids and bases (a) as colour former, together with an oxidisable coupling component, in the Trinder reaction for the detection of (a) hydrogen perox- ide or hydrogen peroxide-forming systems or (b) peroxidase or peroxidate-acting substances or (b) together with an oxidation agent for the detection of aromatic amines or of systems for- ming such amines. The present invention also provides agents for the detection of redox sys- tems containing the above aniline derivatives of general formula I. Furthermore, the present in- vention provides new aniline derivatives coming within the ambit of the above general formula I, as well as processes for the preparation thereof. 5084393 CONTAINER FOR A BIOLOGICAL CULTURE Alena Rogalsky PCT No. PCT/US89/03706 Sec. 371 Date Aug. 28, 1989 Sec. 102(e) Date Aug. 28, 1989 PCT Filed Aug. 28, 1989.A container for biological culture (1, 1’) has a wall which limits an inner chamber with an area (2) for growing cells. The wall has a brush-like wall portion (4, 8) with a plurality of bristle-shaped projections (5, 5’) ex- tending into the area (2) for growing cells. The container (1, 1’) has a significantly increased area for growing cells. 5084509 POLYAMIDE BOUND PROTIDE, METHOD OF USE THEREOF AND KIT James T Sparrow, Patrick Kanda, Ronald C Kennedy assigned to Baylor College of Medicine A polyamide resin for use in peptide and protein synthesis, and a method of preparing and using same. The polyamide resin is prepared by mixing a dimethylacrylamide monomer with an N- acrylyl-diaminoalkane functional monomer in an aqueous solution together with a cross-linker and emulsifying the aqueous solution in an or- ganic solvent. An initiator and a promoter are added to polymerize the N-acrylyl- diaminoalkane functional monomer, dimethylacrylamide monomer, and cross-linker in the form of beads. The pH of the mixture is controlled during the polymerization. The beads are used as a solid phase for peptide and protein synthesis according to methods known in the art. The conjugate of the polyamide resin and the synthesized peptide or protein is used directly for immunoassays or immunization without the need for separation of the peptide or protein from the resin and subsequent purification.

5084393 Container for a biological culture

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PATENT ABSTRACTS 294

5084381

ASSAY METHOD FOR DETECTING HYDROGEN

PEROXIDE

Kengo Akimoto, Sumio Asami, Yukikaz Saeki, Osaka, Japan assigned to Suntory Limited

An improved method of chemiluminescence as- say for hydrogen peroxide wherein a peroxidase of microorganism origin is used in place of hor- seradish peroxidase. This allows an assay of unexpectedly high sensitivity to be achieved.

5084382

AGENT AND PROCESS FOR THE DETECTION OF REDOX

SYSTEMS

Gunte Frey, Gerd Zimmermann, Ellerstadt, Federal Republic Of Germany assigned to Boehringer Mannheim GmbH

The present invention is concerned with the use of aniline derivatives of the general formula: See Patentfor Chemical Structure (I) wherein Rl is a hydrogen atom or a lower alkyl or aryl radical, R2 is a hydrogen atom or a lower alkyl radical which can be substituted by hydroxyl, amino, carboxyl, lower alkoxycarbonyl, lower alkanoylamido, aryl lower alkyl or aryl or by a group of the structure: See Patent for Chemical Strucfure in which R5 and R6 have the meanings given hereinafter, R3 is a hydrogen or halogen atom or a carboxyl group, R4 and R’4, which can be the same or different, are hydrogen or halogen atoms, carboxyl groups or lower alkoxy radicals or lower alkyl radicals which are pre- ferably in the m-position and Rl and R4 or R2 and R4 or R4 and R’4, when the two substituents are ortho to one another, can together form a saturated or unsaturated hydrocarbon chain containing 2 to 6 carbon atoms which can be substituted by hydroxyl or 0x0 groups, R5 is a hydrogen atom or a lower alkyl radical and R6 is a hydroxyl group or a lower alkoxy, lower alkyl or aryl radical; and of the salts thereof with acids and bases (a) as colour former, together with an oxidisable coupling component, in the Trinder reaction for the detection of (a) hydrogen perox- ide or hydrogen peroxide-forming systems or (b) peroxidase or peroxidate-acting substances or (b) together with an oxidation agent for the detection of aromatic amines or of systems for- ming such amines. The present invention also

provides agents for the detection of redox sys- tems containing the above aniline derivatives of general formula I. Furthermore, the present in- vention provides new aniline derivatives coming within the ambit of the above general formula I, as well as processes for the preparation thereof.

5084393

CONTAINER FOR A BIOLOGICAL CULTURE

Alena Rogalsky

PCT No. PCT/US89/03706 Sec. 371 Date Aug. 28, 1989 Sec. 102(e) Date Aug. 28, 1989 PCT Filed Aug. 28, 1989.A container for biological culture (1, 1’) has a wall which limits an inner chamber with an area (2) for growing cells. The wall has a brush-like wall portion (4, 8) with a plurality of bristle-shaped projections (5, 5’) ex- tending into the area (2) for growing cells. The container (1, 1’) has a significantly increased area for growing cells.

5084509

POLYAMIDE BOUND PROTIDE, METHOD OF USE THEREOF AND

KIT

James T Sparrow, Patrick Kanda, Ronald C Kennedy assigned to Baylor College of Medicine

A polyamide resin for use in peptide and protein synthesis, and a method of preparing and using same. The polyamide resin is prepared by mixing a dimethylacrylamide monomer with an N- acrylyl-diaminoalkane functional monomer in an aqueous solution together with a cross-linker and emulsifying the aqueous solution in an or- ganic solvent. An initiator and a promoter are added to polymerize the N-acrylyl- diaminoalkane functional monomer, dimethylacrylamide monomer, and cross-linker in the form of beads. The pH of the mixture is controlled during the polymerization. The beads are used as a solid phase for peptide and protein synthesis according to methods known in the art. The conjugate of the polyamide resin and the synthesized peptide or protein is used directly for immunoassays or immunization without the need for separation of the peptide or protein from the resin and subsequent purification.