2
292 PATENT ABSTRACTS 4978749 4980282 NON-RADIOACTIVE LABELLED SINGLE-STRANDED PROBE, METHOD FOR MANUFACTURING IT AND METHOD FOR DETECTING A SPECIFIED NUCLEOTIDE SEQUENCE USING THIS PROBE Avrameas Stratis, Hiroshi Sakamoto, Francois Traincard, Quang Tuyen Vo, Jean-Luc Gues- don, Therese Ternynck, Pards, France assigned to Institute Pasteur; Centre National de la Recherche Scientifiq TREATMENT OF CORN STEEP LIQUOR Troostembergh Jean-Claude de, Francoise Oudeene, Tielt Winge, Belgium assigned to CPC International Inc A process for the treatment of corn steepwater. The steepwater is incubated for a sufficient time to develop biomass before corn steepwater is fed continuously to the developed biomass at a rate such that the residence time of the steepwater in contact with the biomass is in excess of 4 hours. The invention relates to a new probe for detec- ting a specified nucleic acid sequence. It consists of a recombinant DNA containing an insert complementary to the nucleic acid sequence to be detected and nuclecic acid sequences derived from the DNA (+) of phage M13, the bases which participate in the composition of this recombinant DNA, in particular the thymine groups, being replaced by 5-bromouracil groups. The invention also relates to a method for manu- facturing this probe, this method comprising the culturing of cell hosts, more especially E. coli, which are auxotrophic for thymine in a medium containing a limiting concentration of thymine and containing 5-bromouracil. After the re- quisite incubation time, the superuatant is removed and the probe is recovered from the bacterial cells. 4980293 DISPENSING REAGENTS IN A SPECIMEN WELL David Jeffs assigned to Multi-Technology Inc A biological specimen-containing and/or rea- gent mixing well used in centrifuge tubes, micro- titre plates or trays and the like, and related methods, which comprises shelves or ledges within the specimen well each of which com- prises a substantial nonvertical surface compo- nent, and functions to receive and temporarily store a desired quantity of reagent against pre- mature delivery ot the bottom region of the well thereby accommodating dispensing of the rea- gent into the bottom region at precisely the desired and proper point in time. 4980281 METHOD OF SCREENING FOR PROTEIN INHIBITORS AND ACTIVATORS Gerard M Housey Inhibitors and activators of a protein whose ex- pression affects a phenotypic characteristic of the cell, especially a cultural or morphological characteristic, are identified by their more pro- nounced effect on cells producing higher, usually non-naturally occurring, levels of the protein, than on cells producing little or none of the pro- tein. In a preferred assay, the effect is observable with the naked eye. By this method, tamoxifen is identified as an inhibitor of PKC activity in cell culture. 4981784 RETINOIC ACID RECEPTOR METHOD Ronald M Evans, Estelita Ong, Prudimar S Segui, Catherine C Thompson, Kazuhiko Umesono, Vincent Giguere assigned to The Salk Institute for Biological Studies A novel retinoic acid receptor is disclosed. The novel receptor is encoded for by cDNA carried on plasmid phRAR1, which has been deposited with the American Type Culture Collection for patent purposes. Chimeric receptor proteins are also disclosed. The chimera are constructed by exchanging functional domains between the glucocorticoid, the mineralocorticoid, the estrogen-related, the thyroid and the retinoic acid receptors. In addition, a novel method for

4981784 Retinoic acid receptor method

  • Upload
    vanthu

  • View
    217

  • Download
    2

Embed Size (px)

Citation preview

Page 1: 4981784 Retinoic acid receptor method

292 PATENT ABSTRACTS

4978749 4980282

N O N - R A D I O A C T I V E L A B E L L E D S I N G L E - S T R A N D E D P R O B E ,

M E T H O D F O R M A N U F A C T U R I N G I T A N D

M E T H O D F O R D E T E C T I N G A S P E C I F I E D N U C L E O T I D E

S E Q U E N C E U S I N G T H I S P R O B E

Avrameas Stratis, Hiroshi Sakamoto, Francois Traincard, Quang Tuyen Vo, Jean-Luc Gues- don, Therese Ternynck, Pards, France assigned to Institute Pasteur; Centre National de la Recherche Scientifiq

T R E A T M E N T O F C O R N S T E E P L I Q U O R

Troostembergh Jean-Claude de, Francoise Oudeene, Tielt Winge, Belgium assigned to CPC International Inc

A process for the treatment of corn steepwater. The steepwater is incubated for a sufficient time to develop biomass before corn steepwater is fed continuously to the developed biomass at a rate such that the residence time of the steepwater in contact with the biomass is in excess of 4 hours.

The invention relates to a new probe for detec- ting a specified nucleic acid sequence. It consists of a recombinant DNA containing an insert complementary to the nucleic acid sequence to be detected and nuclecic acid sequences derived from the DNA (+) of phage M13, the bases which participate in the composition of this recombinant DNA, in particular the thymine groups, being replaced by 5-bromouracil groups. The invention also relates to a method for manu- facturing this probe, this method comprising the culturing of cell hosts, more especially E. coli, which are auxotrophic for thymine in a medium containing a limiting concentration of thymine and containing 5-bromouracil. After the re- quisite incubation time, the superuatant is removed and the probe is recovered from the bacterial cells.

4980293

D I S P E N S I N G R E A G E N T S I N A S P E C I M E N W E L L

David Jeffs assigned to Multi-Technology Inc

A biological specimen-containing and/or rea- gent mixing well used in centrifuge tubes, micro- titre plates or trays and the like, and related methods, which comprises shelves or ledges within the specimen well each of which com- prises a substantial nonvertical surface compo- nent, and functions to receive and temporarily store a desired quantity of reagent against pre- mature delivery ot the bottom region of the well thereby accommodating dispensing of the rea- gent into the bottom region at precisely the desired and proper point in time.

4980281

M E T H O D O F S C R E E N I N G F O R P R O T E I N I N H I B I T O R S A N D

A C T I V A T O R S

Gerard M Housey

Inhibitors and activators of a protein whose ex- pression affects a phenotypic characteristic of the cell, especially a cultural or morphological characteristic, are identified by their more pro- nounced effect on cells producing higher, usually non-naturally occurring, levels of the protein, than on cells producing little or none of the pro- tein. In a preferred assay, the effect is observable with the naked eye. By this method, tamoxifen is identified as an inhibitor of PKC activity in cell culture.

4981784

R E T I N O I C A C I D R E C E P T O R M E T H O D

Ronald M Evans, Estelita Ong, Prudimar S Segui, Catherine C Thompson, Kazuhiko Umesono, Vincent Giguere assigned to The Salk Institute for Biological Studies

A novel retinoic acid receptor is disclosed. The novel receptor is encoded for by cDNA carried on plasmid phRAR1, which has been deposited with the American Type Culture Collection for patent purposes. Chimeric receptor proteins are also disclosed. The chimera are constructed by exchanging functional domains between the glucocorticoid, the mineralocorticoid, the estrogen-related, the thyroid and the retinoic acid receptors. In addition, a novel method for

Page 2: 4981784 Retinoic acid receptor method

PATENT ABSTRACTS

identifying functional ligands for receptor pro- teins is disclosed. The method, which takes ad- vantage of the modular structure of the hormone receptors and the idea that the functional domains may be interchangeable, replaces the DNA-binding domain of a putative novel recep- tor with the DNA-binding domain of a known receptor such as the glucocorticoid receptor. The resulting chimeric construction, when expressed in cells, produces a hybrid receptor whose activa- tion of a ligand-(e.g., glucocorticoid) inducible promoter is dependent on the presence of the new ligand. The novel method is illustrated in part by showing that the ligand for the new receptor protein is the retinoid, retinoic acid.

4981785

A P P A R A T U S A N D M E T H O D F O R P E R F O R M I N G I M M U N O A S S A Y S

P Narayan Nayak assigned to Ventrex Laboratories Inc

An apparatus and method for performing enzyme-linked immunological sandwich assays (ELISA) by affinity chromatography. Forma- tion of the antibody-antigen-conjugate complex, and the development of a chromatophore in- dicator take place in a semi-automated, walk- away, fashion. The immunoassay apparatus comprises a chromatography tank, which fur- ther comprises a reaction compartment and a plurality of liquid solvent reservoirs, with a first- liquid reservoir being configured higher than a second-liquid reservoir. The apparatus allows automatic sequential and discrete movement of wash solution, followed by a color development solution, with unbound conjugate being sub- stantially washed away from the immunological sandwich complex before the color development solution automatically comes into contact with the immunological complex.

4981790

S T A B L E T A T I I I C E L L L I N E S , T A T I I I G E N E P R O D U C T S , A N D

A S S A Y M E T H O D S

William A Haseltine, Craig A Rosen, Joseph Sodroski, Wei C Goh assigned to Dana Farber Cancer Institute

This invention describes stable tatlII cell lines. It is disclosed that by transfecting a preselected

293

tatlII cell line with a vector containing a suffic- ient amount of the HTLV-III LTR responsive to tatlII gene products for trans-activation and an enhancer upstream of the tatllI responsive seg- ment, it is possible to express high levels of the tatlII gene products. By including a preselected heterologous gene on this vector, it is also poss- ible to express high levels of a desired gene pro- duct. A substantially pure protein comprising 86 amino acids and having an apparent molecular weight of about 14,000 dalton and exhibiting trans-activating activity is also disclosed. This protein and polypeptides having trans- activating ability, which is also disclosed, can be used to produce high levels of a desired gene pro- duct. A method of detecting the presence of HTLV-III/LAV virus in an individual is also dis- closed and comprises the step of: (a) incubating whole blood or lymphocytes from the invididual to be tested with tatlII cell lines of the present in- vention in a culture medium; and (b) screening for cytopathic effects on the cells is also dis- closed. A method of screening for a compound that inhibits trans-activation of the tatlII gene product is also disclosed and comprises the steps of: (1) transacting a tatlII cell line of the present invention with a vector containing a gene that expresses a selectable marker and whose expres- sion is under the control of an HTLV-III LTR; (2) transfecting the same type of tatlII cell lines as in step (1) with the selectable gene chosen in step (1) but under the control of a different regulatory sequence; (3) thereafter, adding a pre- selected compound to each of the cell lines in in- creasing concentrations; and (4) measuring the expression of the selectable gene product to determine whether the compound effects the tatlII function without being toxic to the cell.

4 ~ 1 8 ~

M E T H O D F O R C U L T U R I N G M I C R O O R G A N I S M

Akira Inoue, Kouki Horikoshi, Tokyo, Japan assigned to Research Development Corporation of Japan

A method for culturing microorganisms belonging to the genus Pseudomonas or the genus Escherichia and having tolerance to an or- ganic solvent such as any one of hydrocarbons, alcohols, ethers, ketones and their derivatives or their mixture in a medium containing the organic solvent in a concentration of 0.3% or more. The present method can be widely utilized in the fields of bioreactor, liquid-waste treatment, pro- tein engineering, etc.