1
PATENT is contained and retained within the pores of the polymeric material and is adapted in use to inter- act with a reactive species and can be made by depositing and retaining the gel or a material adapted in use to form the gel within the pores of the porous polymeric material. The high porosity of the porous polymeric material in combination with the retention of the gel within the pores permit high loading capacities, par- ticularly in the area of peptide synthesis to be ac- hieved. The substrate can be employed in chemical synthesis, chromatography techniques, ion exchange and separation techniques. 4966792 METHOD OF PRODUCING GRADIENT GEL MEDIUM MEMBRANE FOR ELECTROPHORESIS Fumitak Terai, Kimio Yukawa, Mineo Suefuji, Kanagawa, Japan assigned to Fuji Photo Film Co Ltd A method for producing gradient gel medium membrane for electrophoresis for determining the base sequence of DNA or DNA partially decomposed material providing an improved productivity. High and low concentration monomer solutions are mixed with a pre- determined quantity of polymerizing reaction in- itiator solution by a static mixer to prepare a gel forming solution for coating on a continuously moving web. The flow-rate ratio of the high and low concentration monomer solutions is gradually changed so as to vary the concentra- tion of the monomer in the gel forming solution alternatively from low to high and from high to low along the web. 4966848 ISOLATION, PURIFICATION, CHARACTERIZATION, CLONING AND SEQUENCING OF N ALPHA- ACETYLTRANSFERASE John A Smith, Fang-Jen S Lee assigned to The General Hospital Corporation This invention is directed to N alpha- acetyltransferase with a molecular weight of ab- out 180,000 daltons, said N alpha- acetyltransferase being composed of two subunit peptides having molecular weights of about 95,000 each, having enzyme activity greater than ABSTRACTS 99 100 wherein one unit of activity is defined as 1 pmol of acetyl residues incorporated into adrenocorticotropic hormone (ACTH) (amino acids 1-24) under standard assay conditions. This invention is further directed to a method for purifying the N alpha-acetyltransferase. 4966853 CELL CULTURING APPARATUS Shoichi Matsuda, Akira Suzuki, Tatsuo Kaise, Tokyo, Japan assigned to Kirin Beer Kabushiki Kaisha A cell culturing apparatus and a cell culturing method are disclosed. A rack supporting ap- paratus includes a loop tracking and a plurality of culturing racks connected one after another in series. Each of the culturing racks ac- commodates culturing containers therein for cell culturing during their travel on the loop tracking. Each of the racks is accessible, through conveyors, to a container handling station where culture medium is filled in the culturing con- tainers and cell inoculation is carried out. The culturing containers processed in the container handling station are automatically ac- commodated into the rack by an infeed station for starting cell culturing, and the culturing con- tainers in which cell culturing have been performed in the rack are automatically dis- charged therefrom by a discharge station. 4968602 SOLUTION-PHASE SINGLE HYBRIDIZATION ASSAY FOR DETECTING POLYNUCLEOTIDE SEQUENCES Nanibhushan Dattagupta assigned to Molecular Diagnostics Inc A process for determining the presence of a par- ticular nucleic acid sequence in a test sample comprising (a) chemically modifying nucleic ac- ids in the test sample either to introduce a label or a reactive site in a manner that supports their hybridizability, (b) contacting under hybridiza- tion conditions the chemically modified sample nucleic acids with a hybridizable nucleic acid probe which either, when the sample nucleic ac- ids have been modified to introduce a label, carrys a reactive site or, when the sample nucleic acids have been modified to introduce a reactive site, is labeled, (c) contacting the solution resul-

4966792 Method of producing gradient gel medium membrane for electrophoresis

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PATENT

is contained and retained within the pores of the polymeric material and is adapted in use to inter- act with a reactive species and can be made by depositing and retaining the gel or a material adapted in use to form the gel within the pores of the porous polymeric material. The high porosity of the porous polymeric material in combination with the retention of the gel within the pores permit high loading capacities, par- ticularly in the area of peptide synthesis to be ac- hieved. The substrate can be employed in chemical synthesis, chromatography techniques, ion exchange and separation techniques.

4966792

M E T H O D O F P R O D U C I N G G R A D I E N T G E L M E D I U M

M E M B R A N E F O R E L E C T R O P H O R E S I S

Fumitak Terai, Kimio Yukawa, Mineo Suefuji, Kanagawa, Japan assigned to Fuji Photo Film Co Ltd

A method for producing gradient gel medium membrane for electrophoresis for determining the base sequence of DNA or DNA partially decomposed material providing an improved productivity. High and low concentration monomer solutions are mixed with a pre- determined quantity of polymerizing reaction in- itiator solution by a static mixer to prepare a gel forming solution for coating on a continuously moving web. The flow-rate ratio of the high and low concentration monomer solutions is gradually changed so as to vary the concentra- tion of the monomer in the gel forming solution alternatively from low to high and from high to low along the web.

4966848

I S O L A T I O N , P U R I F I C A T I O N , C H A R A C T E R I Z A T I O N , C L O N I N G A N D S E Q U E N C I N G O F N A L P H A -

A C E T Y L T R A N S F E R A S E

John A Smith, Fang-Jen S Lee assigned to The General Hospital Corporation

This invention is directed to N alpha- acetyltransferase with a molecular weight of ab- out 180,000 daltons, said N alpha- acetyltransferase being composed of two subunit peptides having molecular weights of about 95,000 each, having enzyme activity greater than

ABSTRACTS 99

100 wherein one unit of activity is defined as 1 pmol of acetyl residues incorporated into adrenocorticotropic hormone (ACTH) (amino acids 1-24) under standard assay conditions. This invention is further directed to a method for purifying the N alpha-acetyltransferase.

4966853

C E L L C U L T U R I N G A P P A R A T U S

Shoichi Matsuda, Akira Suzuki, Tatsuo Kaise, Tokyo, Japan assigned to Kirin Beer Kabushiki Kaisha

A cell culturing apparatus and a cell culturing method are disclosed. A rack supporting ap- paratus includes a loop tracking and a plurality of culturing racks connected one after another in series. Each of the culturing racks ac- commodates culturing containers therein for cell culturing during their travel on the loop tracking. Each of the racks is accessible, through conveyors, to a container handling station where culture medium is filled in the culturing con- tainers and cell inoculation is carried out. The culturing containers processed in the container handling station are automatically ac- commodated into the rack by an infeed station for starting cell culturing, and the culturing con- tainers in which cell culturing have been performed in the rack are automatically dis- charged therefrom by a discharge station.

4968602

S O L U T I O N - P H A S E S I N G L E H Y B R I D I Z A T I O N A S S A Y F O R

D E T E C T I N G P O L Y N U C L E O T I D E S E Q U E N C E S

Nanibhushan Dattagupta assigned to Molecular Diagnostics Inc

A process for determining the presence of a par- ticular nucleic acid sequence in a test sample comprising (a) chemically modifying nucleic ac- ids in the test sample either to introduce a label or a reactive site in a manner that supports their hybridizability, (b) contacting under hybridiza- tion conditions the chemically modified sample nucleic acids with a hybridizable nucleic acid probe which either, when the sample nucleic ac- ids have been modified to introduce a label, carrys a reactive site or, when the sample nucleic acids have been modified to introduce a reactive site, is labeled, (c) contacting the solution resul-