PATENT ABSTRACTS 411

lent temperatures, intact and in a sterile, sealed gents, catalysts, polymeric reagents, condition, via common carrier to a central chromatographic supports, and as enzyme-or laboratory, where the portion of the second filter other biomacromolecule-bearing supports. is removed for testing. Thus, the present device is Novel carboxylate-functional polymer beads, a simple to use and inexpensive means for con- are intermediates in the preparation of the ducting microbiological testing of samples from azlactone-functional beads. Azlactone- remote sites. It eliminates the need for functional beads have units of the formula: See laboratory personnel to take and filter and/or Patent for Chemical Structure V wherein R I is H take and chill and filter a sample at the site of or CH3, R2 and R3 independently can be an testing and for mobile laboratory testing faci- alkyl group having 1 to 14 carbon atoms, a cyclo- lities, or alternatively, the need for special ex- alkyl group having 3 to 14 carbon atoms, an aryl press shipping arrangements for liquid unfiltered group having 5 to 12 ring atoms, an arenyl group water samples, having 6 to 26 carbon and 0 to 3 S, N, and non-

pcroxidic O heteroatoms, or R2 and R3 taken together with the carbon to which they are joined

4871674 can form a carbocyclic ring containing 4 to 12 ring atoms, and n is an integer 0 or 1, the azlac-

C U L T U R E C E L L tone functional beads having more than 5 and up to 99 molar parts of crosslinking monomer in-

Shigeak Matsui, Eiichi Hirai, Junji Nakamura, corporated therein. Hirakata, Japan assigned to Kurashiki Boseki Kabushiki Kaisha

4871838 A culture cell having discontinuous projecting parts for hanging the culture cell on an upper cir- P R O B E S A N D M E T H O D S F O R cumferential portion and a membrane filter on a D E T E C T I N G A C T I V A T E D R A S bottom portion, characterized in that triangular O N C O G E N E S tapered ribs are provided continuously under-

neath the discontinuous projecting parts. Johannes L Bos, Eb Alex J Van der, Leiden,

Netherlands assigned to The Board of Rijks 4871676 Universiteit Leiden

Molecules complementary to nucleotide C E L L C U L T U R E I N C U B A T O R sequences encoding mutant ras protiens which

contain a single-base mutation in the codon Koji Yamada, Osaka, Japan assigned to encoding amino acids at position 13, 12 or 61 Sumitomo Electric Industries Ltd have been produced. These molecules are useful

in methods of detecting specific single-base An incubator for cell culture used in the field of mutations in altered ras genes and the specific biotechnology, in which a driving mechanism for cancers associated with such mutations. rotating a tray stock for supporting a plurality of trays for cell culture is provided outside an in- cubator housing. 4872987

P R O C E S S F O R S E P A R A T I N G A N D 4871824 P R O D U C I N G C H L O R O G E N I C

A C I D V A R I A B L Y C R O S S L I N K E D

P O L Y M E R I C S U P P O R T S Rciner Kopsch, Claus F Gosswein, Henning Lutz, Michael Ball, Peter Hubert, Schenefeld,

Steven M Heilmann, Jcrald K Rasmussen, Larry Federal Republic Of Germany assigned to Ergo R Krepski, Dean S Milbrath, Patrick L Coleman Forschungsgescllschaft mbH assigned to Minnesota Mining and Manu- facturing Company Chlorogenic acid can be separated and produced

as free acid or as salts thereof from plant raw Azlactone-functional polymer beads are useful material extracts by means of gel permeation reactive supports for the attachment of rune- chromatography on cross-linked modifiedpoly- tional materials to provide novel adduct beads, saceharides, especially on dextrans. To obtain The adduct beads are useful as complexing a- the free acid, the aqueous extract must be ad-