458 PATENT ABSTRACTS

hybrid cells formed by fusing the TP-3 cell line An affinity matrix and a method for the detec- with antibody-producing cells from immunized tion of low molecular weight compositions such rabbits, which hybrid cells are hypoxyxanthine as attatoxins are provided utilizing specific aminopterine thymidine insensitve and secrete monoclonal IgM antibody having an affinity rabbit antibodies, and the methods for preparing constant not less than about 1" 109 liters per the hybrid cells and hybrid cell lines. The inven- mole. Methods for the preparation and use of tion further comprises rabbit monoclonal anti- such affinity matrices are also given. The detec- body. tion is rapid, accurate, reproducible, and allows

for quantitative recovery of the composition of interest.

4859598

A N T I B I O T I C L L - D 4 2 0 6 7 £ 6 2 4859613

Guy T Carter, Donald B Borders, Joseph J I M M U N O A S S A Y S F O R Goodman, David Labeda assigned to American G L U T A T H I O N E A N D Cyanamid Company A N T I B O D I E S U S E F U L T H E R E I N

Antibiotic LL-D42067 beta derived by aerobic David A Lawrence fermentation of the microorganism Ac- tinomadura madurae subspecies simaoensis Monoclonal antibodies specifically immuno- NRRL 15734, useful as an antibacterial and logically reactive to thiol-modified glutathione antiparasitic agent, and hybridoma cell lines producing such mono-

clonal antibodies. A method of producing antibodies specifically immunologieally reactive

4859599 with reduced glutathione by immunizing an animal using a thiol-modified glutathione, for

A N T I B I O T I C A26201-1 A N D example, a glutathione-N-ethylmaleimide key- hole limpet hemocyanin conjugate. A method of

ANTIBIOTIC A26201-2 PRODUCED utilizing the antibodies produced to quantitate BY A N O V E L S T R A I N O F the amount of reduced glutathione in a bio-

A C T I N O P L A N E S logical sample, to monitor glutathione- associated conditions, to monitor the formation

Joseph W Whalen, Gregory L Swartz, Lisa of normal metabolic intermediates and to Rheaume, Karen McCoy assigned to The Dow monitor the detoxification of foreign com- Chemical Company pounds.

Antibiotic A26201-1 and antibiotic A26201-2 are produced by the novel microorganism, Ac- 4859765 tinoplanes species A26201 (ATCC 39573), under aqueous aerobic fermentation conditions. The S Y N T H E T I C P E P T I D E antibiotics are useful for inhibiting bacteria, par- S E Q U E N C E S U S E F U L IN ticularly gram positive bacteria, and are also use- B I O L O G I C A L A N D ful for promoting the growth of monogastric and P H A R M A C E U T I C A L ruminant animals. A P P L I C A T I O N S A N D M E T H O D S

O F M A N U F A C T U R E

4859611 John Nestor, John G Moffatt, Hardy Chan as-

A F F I N I T Y C O L U M N A N D sigrled to Syntex (U S A ) Inc

P R O C E S S F O R D E T E C T I O N O F The present invention relates to novel synthetic L O W M O L E C U L A R W E I G H T antigens, conjugates and antibodies based upon

T O X I C S U B S T A N C E S specific peptide sequences and the production thereof. More particularly, the invention relates

John Groopman, Gerald Wogan, Frederick Bar- to a polypeptide which is a de t erminant site o f a goot, Christophe Ferrari assigned to Mas- protein, the polypeptide having from 8 to 20 sachusetts Institute of Technology; Boston amino acid residues, having an amino-terminal Universi amino acid and a carboxyl-terminal amino acid,