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452 PATENT ABSTRACTS A fusion between DNA sequences coding for hAT and hGRF via a synthetic adaptor coding for an in vitro cleavable amino acid sequence is used to express hCRF at high levels in E. coli. 4828989 PROCESS FOR PURIFYING A PROTEIN Chrisopher P Prior. Garance M Ducommun A process for purifying a protein and par- ticularly the purification of a protein, such as gamma interferon, that forms highly insoluble aggregates during the growth of host cells trans- formed with DNA sequence coding for the pro- tein. 4828990 METHOD FOR PURIFYING AN INTERFERON Naoki Higdshi, Shunjir Sugimoto, Masafumi Tsujimoto, Hounai Shirasawa, Tsutomu Okada, Kazumori Yamamoto, Tattanahalli L Nagab- hushan, Paul P Trotta, Toyonaka shi, Osaka, Japan A method of purifying a polypeptide havmg a physiological activity such as one having inter- feron activities from a culture mixture of a microorganism obtained by a recombinant DNA technique and capable of producing the polypeptide is disclosed. The method comprises subjecting the cultured cells to extraction and purification in the presence of a salt of zinc or copper and polyethyleneimine thereby inhibiting decomposition and denaturation of the poly- peptide. The extracted polypeptide can be fur- ther purified by column chromatographies using a column containing an anion exchange resin, column containing a cation exchange resin and column containing a gel filtration resin. 4828991 TUMOR SPECIFIC MONOCLONAL ANTIBODIES Michael Hanna, Martin V Haspel, Herbert C Hoover assigned to Akzo N V Monoclonal antibodies produced by hybridoma or transformed B-cell lines deriveed from B-cells of cancer patients actively immunized with auto- logous tumor antigen. These monoclonal anti- bodies can be used in both diagnostic procedures and therapy for human cancers. 4828995 HYBRID CELL LINE FOR PRODUCING MONOCLONAL ANTIBODY TO A HUMAN THYMOCYTE ANTIGEN, ANTIBODY, AND METHODS Patrick C S Kung, Gideon Goldstein assigned to Ortho Pharmaceutical Corporation Hybrid cell line for production of monoclonal antibody to an antigen found on approximately 70% of normal human thymocytes. The hybrid is formed by fusing splenocytes from immunized CAFl mice with P3X63AgXUi myeloma cells. Diagnostic and therapeutic uses of the mono- clonal antibody are also disclosed. 4830858 SPRAY-DRYING METHOD FOR PREPARING LIPOSOMES AND PRODUCTS PRODUCED THEREBY Nicholas I Payne, J Roge Salmon, Wirral. United Kingdom assigned to E R Squibb & Sons Inc A method IS provided for preparing a stable lipo- some precursor in the form ofa mixture of spray- dried liposomal components mcluding one or more biologically active compounds which may be stored dry and reconstituted with water to form a liposomal preparation immediately prior to use. The dry liposome precursor is also pro- vided. 4830967 PROCESS FOR PRODUCING ANTIBIOTIC A80438 Robert L Hamill, Raymond Yao assrgned to Eli Lilly and Company A new stram of Streptomyces pactum, NRRL 15970, and an improved fermentation process for producing the polyether antibiotic A80438 using this microorganism are provided.

4830967 Process for producing antibiotic A80438

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Page 1: 4830967 Process for producing antibiotic A80438

452 PATENT ABSTRACTS

A fusion between DNA sequences coding for hAT and hGRF via a synthetic adaptor coding for an in vitro cleavable amino acid sequence is used to express hCRF at high levels in E. coli.

4828989

PROCESS FOR PURIFYING A PROTEIN

Chrisopher P Prior. Garance M Ducommun

A process for purifying a protein and par- ticularly the purification of a protein, such as gamma interferon, that forms highly insoluble aggregates during the growth of host cells trans- formed with DNA sequence coding for the pro- tein.

4828990

METHOD FOR PURIFYING AN INTERFERON

Naoki Higdshi, Shunjir Sugimoto, Masafumi Tsujimoto, Hounai Shirasawa, Tsutomu Okada, Kazumori Yamamoto, Tattanahalli L Nagab- hushan, Paul P Trotta, Toyonaka shi, Osaka, Japan

A method of purifying a polypeptide havmg a physiological activity such as one having inter- feron activities from a culture mixture of a microorganism obtained by a recombinant DNA technique and capable of producing the polypeptide is disclosed. The method comprises subjecting the cultured cells to extraction and purification in the presence of a salt of zinc or copper and polyethyleneimine thereby inhibiting decomposition and denaturation of the poly- peptide. The extracted polypeptide can be fur- ther purified by column chromatographies using a column containing an anion exchange resin, column containing a cation exchange resin and column containing a gel filtration resin.

4828991

TUMOR SPECIFIC MONOCLONAL ANTIBODIES

Michael Hanna, Martin V Haspel, Herbert C Hoover assigned to Akzo N V

Monoclonal antibodies produced by hybridoma or transformed B-cell lines deriveed from B-cells

of cancer patients actively immunized with auto- logous tumor antigen. These monoclonal anti- bodies can be used in both diagnostic procedures and therapy for human cancers.

4828995

HYBRID CELL LINE FOR PRODUCING MONOCLONAL

ANTIBODY TO A HUMAN THYMOCYTE ANTIGEN,

ANTIBODY, AND METHODS

Patrick C S Kung, Gideon Goldstein assigned to Ortho Pharmaceutical Corporation

Hybrid cell line for production of monoclonal antibody to an antigen found on approximately 70% of normal human thymocytes. The hybrid is formed by fusing splenocytes from immunized CAFl mice with P3X63AgXUi myeloma cells. Diagnostic and therapeutic uses of the mono- clonal antibody are also disclosed.

4830858

SPRAY-DRYING METHOD FOR PREPARING LIPOSOMES AND

PRODUCTS PRODUCED THEREBY

Nicholas I Payne, J Roge Salmon, Wirral. United Kingdom assigned to E R Squibb & Sons Inc

A method IS provided for preparing a stable lipo- some precursor in the form ofa mixture of spray- dried liposomal components mcluding one or more biologically active compounds which may be stored dry and reconstituted with water to form a liposomal preparation immediately prior to use. The dry liposome precursor is also pro- vided.

4830967

PROCESS FOR PRODUCING ANTIBIOTIC A80438

Robert L Hamill, Raymond Yao assrgned to Eli Lilly and Company

A new stram of Streptomyces pactum, NRRL 15970, and an improved fermentation process for producing the polyether antibiotic A80438 using this microorganism are provided.