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294 PATENT ABSTRACTS bodies to the hapten for assay use or in the manufacture of vaccines; and (b) a desired poly- peptide product and additional protein from which the desired product may be cleaved; and 3. Methods of preparing synthetic structural genes coding for the expression of mammalian poly- peptides in microbial cloning systems. 4567143 PROCESS FOR PREPARING 4'-DESCHLOROREBECCAMYCIN James A Matson assigned to Bristol-Myers Company 4565785 RECOMBINANT DNA MOLECULE A new antitumor antibiotic designated herein as 4'-deschlororebeccamycin is produced by fer- mentation of Nocardia aerocolonigenes ATCC 39243. The new compound possesses anti- bacterial activity and inhibits the growth of tumors in experimental animals. Walte Gilbert, Stephanie A Broome, Lydia J Villa-Komaroff, Argiris A Efstratiadis assigned to The President and Fellows of Harvard College A plasmid or phage gene for a periplasmic or extraceUular bacterial protein is cleaved, a double-stranded DNA sequence coding for a selected protein or portion thereof from a eu- karyotic cell such as insulin is inserted in that cleaved gene by recombinant DNA techniques and used to transform a bacterium, and the ex- creted selected protein is collected. 4567141 PLASMID VECTORS INCLUDING TN904 Ronald H Olsen assigned to Microlife Technics Inc Cloning vectors are described which include the streptomycin resistance (Smr) determinant derived from Tn904. A single site for the restric- tion endonuclease, AvaI, is present within the Tng04 determinant for Smr. A method is described for preparing the Tn904 containing cloning vectors through transposition of Tn904 to a parent cloning vector and then cloning of the Smr gene into another vector segment. The cloning vector is important for inserting deoxy- ribonucleic acid segments, which encode for various characteristics such as chemical produc- tion, antibiotic resistance or bacterial cell pro- perties, in the Star gene Aval cleaved site and which normally provides a marker for identifica- tion of transformed strains of bacteria. 4567145 CONTINUOUS PRODUCTION OF ETHANOL BY USE OF RESPIRATION DEFICIENT MUTANT YEAST Marcel Faber, Jerome D Bernstein, Matthew Grossman assigned to HRI Inc This invention provides a process for producing ethanol from a D-sugar in a continuous aerobic environment using a flocculant respiration- deficient mutant of Saccharomyces uvarum in a single stage fermentor with a cell settling tank and cell recycle, at a productivity of more than 50 grams ethanol per liter fermentor volume per hour. The process comprises (a) innoculating a fermentation zone with a respiration-deficient mutant of Saccharomyces uvarum; (b) feeding a mixture of a D-sugar, a nitrogen source, a vitamin source and a mineral source into the fer- mentation zone in the presence of oxygen, and (c) fermenting the D-sugar mixture for a suffic- iently long period of time to yield an ethanol product. In the process, the yeast are allowed to settle for a sufficiently long period of time and recycled to the fermentation zone to increase ethanol productivity. The preferred D-sugar that may be used in the present process to produce ethanol, is D-glucose. 4568542 VACCINE COMPOSITIONS Lee Kronenberg assigned to Lee BioMolecular Research Laboratories lnc The present invention describes the inactivation of target cells by treatment of said cells with

4567145 Continuous production of ethanol by use of respiration deficient mutant yeast

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294 PATENT ABSTRACTS

bodies to the hapten for assay use or in the manufacture of vaccines; and (b) a desired poly- peptide product and additional protein from which the desired product may be cleaved; and 3. Methods of preparing synthetic structural genes coding for the expression of mammalian poly- peptides in microbial cloning systems.

4567143

P R O C E S S F O R P R E P A R I N G 4 ' - D E S C H L O R O R E B E C C A M Y C I N

James A Matson assigned to Bristol-Myers Company

4565785

R E C O M B I N A N T D N A M O L E C U L E

A new antitumor antibiotic designated herein as 4'-deschlororebeccamycin is produced by fer- mentation of Nocardia aerocolonigenes ATCC 39243. The new compound possesses anti- bacterial activity and inhibits the growth of tumors in experimental animals.

Walte Gilbert, Stephanie A Broome, Lydia J Villa-Komaroff, Argiris A Efstratiadis assigned to The President and Fellows of Harvard College

A plasmid or phage gene for a periplasmic or extraceUular bacterial protein is cleaved, a double-stranded DNA sequence coding for a selected protein or portion thereof from a eu- karyotic cell such as insulin is inserted in that cleaved gene by recombinant DNA techniques and used to transform a bacterium, and the ex- creted selected protein is collected.

4567141

P L A S M I D V E C T O R S I N C L U D I N G TN904

Ronald H Olsen assigned to Microlife Technics Inc

Cloning vectors are described which include the streptomycin resistance (Smr) determinant derived from Tn904. A single site for the restric- tion endonuclease, AvaI, is present within the Tng04 determinant for Smr. A method is described for preparing the Tn904 containing cloning vectors through transposition of Tn904 to a parent cloning vector and then cloning of the Smr gene into another vector segment. The cloning vector is important for inserting deoxy- ribonucleic acid segments, which encode for various characteristics such as chemical produc- tion, antibiotic resistance or bacterial cell pro- perties, in the Star gene Aval cleaved site and which normally provides a marker for identifica- tion of transformed strains of bacteria.

4567145

C O N T I N U O U S P R O D U C T I O N O F E T H A N O L BY U S E O F

R E S P I R A T I O N D E F I C I E N T M U T A N T Y E A S T

Marcel Faber, Jerome D Bernstein, Matthew Grossman assigned to HRI Inc

This invention provides a process for producing ethanol from a D-sugar in a continuous aerobic environment using a flocculant respiration- deficient mutant of Saccharomyces uvarum in a single stage fermentor with a cell settling tank and cell recycle, at a productivity of more than 50 grams ethanol per liter fermentor volume per hour. The process comprises (a) innoculating a fermentation zone with a respiration-deficient mutant of Saccharomyces uvarum; (b) feeding a mixture of a D-sugar, a nitrogen source, a vitamin source and a mineral source into the fer- mentation zone in the presence of oxygen, and (c) fermenting the D-sugar mixture for a suffic- iently long period of time to yield an ethanol product. In the process, the yeast are allowed to settle for a sufficiently long period of time and recycled to the fermentation zone to increase ethanol productivity. The preferred D-sugar that may be used in the present process to produce ethanol, is D-glucose.

4568542

V A C C I N E C O M P O S I T I O N S

Lee Kronenberg assigned to Lee BioMolecular Research Laboratories lnc

The present invention describes the inactivation of target cells by treatment of said cells with