350 PATENT ABSTRACTS

OH OH O

H

1

OH 014 0

Streptomyces sp., NRRL 12049. The A-39183 antibiotics are closely related antibiotics. The in- dividual A-39183 factors are separated by chromatography. The A-39183 factors are anti- bacterial agents which have activity against Staphylococcus and Streptococcus species that are penicillin resistant. The A-39183 factors are also active against both gram-positive and gram- negative anaerobic bacteria, and are ionophores.

4409332

C O L L A G E N - E N Z Y M E C O N J U G A T E S T H A T EXHIBIT NO

I N F L A M M A T O R Y R E S P O N S E AND M E T H O D FOR MA K IN G

T H E S A M E

Steven Jefferies, James L Gutmann, Barry Heat- field

Collagen polymers are rendered non- inflammatory by complexation with alkaline phosphatase according to the procedure dis- closed. Applications of these non-inflammatory collagen complexes include the production of non-inflammatory collagen sutures, collagen soft tissue replacements !ncluding wound and burn coverings and arterial vessel replacements, collagen drug delivery devices, and vitreous replacement for opthalmologic therapy. Gels composed of the collagen-alkaline phosphatase conjugate plus calcium hydroxide are useful as an agent in endodontic therapy. Structural dur- ability and stabilization of the anti- inflammatory properties of the material are enhanced by crosslinking with giutaraldehyde or other crosslinking agents. Glutaraldehyde also acts to sterilize and disinfect the collagen con- jugate prior to in-vivo use.

~ 1 ~ 2 0

3 - A M I N O - ( I ) - B E N Z A Z E P I N - 2 - O N E - I -ALKANOIC ACIDS

Jeffrey W H Watthey assigned to Ciba-Geigy Corporation

Variously substituted l-carboxymethyl-3- (carboxymethylamino)-2,3,4, 5-tetrahydro- i H- (l)benT~Tepin-2-ones and functional derivatives are angiotension converting enzyme inhibitors and are useful as antihypertensive agents. Syn- thesis of, compositions and methods of treat- ment utilizing such compounds are included.

440932.7

P R O C E S S AND RE A G E N T F O R THE D E T E R M I N A T I O N OF T H E

B I O L O G I C A L ACTIVITY OF H E P A R I N IN P L A S M A

Knut Bartl, Helmut Lill, Pete Rocschlau, Joachim Ziegenhorn, Wilzhofen, Federal Republic Of Germany assigned to Boehringer Mannheim GmbH

Method and reagent for the determination of the biological activity of heparin in plasma by adding thrombin or Factor Xa as the proteolytic enzyme and a chromogen substratc of the latter and measuring the dye liberated from the

PATENT ABSTP~CT S 351

chromogen substrate in the absence of ex- ogenous anti-thrombin ili, the improvement consisting of adding, additionally, at least one compound of the formula See Patent for Chem- ical Structure wherein X is oxygen or the group NRS; and RI, R2, R3, R4 and R5 are indepen- dently selected from hydrogen or alkyl of I to 2 carbon atoms.

4409210

said mammal an antigen (ACRT) having im- mune cross-reactivity with human reverse trans- criptase, or detecting antibodies against said ACRT or detecting antibody-ACRT complexes, wherein the reverse transcriptase is substantially purified, has a molecular of about 70.000 and a sedimentation coefficient on a glycerol gradient of between 5 and 5.5 S. A process tbr the purification of reverse transcriptase from human milk.

A N T I B I O T I C C O M P O U N D

Hirosh Kawaguchi, Masataka Konishi, Koko Sugawara, Koji Tomita, Tokyo, Japan assigned to Bristol-Myers Company

CHr~CI4~ i)m~H--C~r'CX3-.D.Pm-.D-r~.r~L.I,m-dL,.Atll

O

L . B - O H - - A ~ ~ L-J , .OH - - lh '~ - - D-St~r - - D-B-OH--A~

A novel water-soluble peptide designated herein as Bu-2517 is produced by fermentation of Em- pedoba~er sp. strain G393-B445 (ATCC 31962). The antibiotic exists in a cyclic depsipep- tide structure and contains the amino acids D- serine, D-proline, L-proline, D-threo- beta- hydroxyaspar tic acid, L-threo- beta- hydroxyaspa'rtic acid, L-arginine and trans-L-3- hydroxyproline and the residue of the C14 fatty acid, 3-hydroxytetradecanoic acid. The Bu-2517 antibiotic inhibits the growth of a variety of aerobic and anaerobic gram-positive bacteria.

4409200

R E V E R S E T R A N S C R I P T A S E F R O M H U M A N M I L K , M E T H O D

F O R I T S P U R I F I C A T I O N , A N D ITS U S E IN T H E D E T E C T I O N O F

B R E A S T C A N C E R

William F Feller, Judith A Kantor, Jack G Chirikjian, Terence M Phillips assigned to Research Corporation

A method for diagnosing mammalian breast cancer by detecting in the physiological fluid of

4407965

P R O C E S S F O R P R E P A R I N G A N T I B O D Y

Nobor Yanaihara, Shizuoka. Japan assigned to Otsuka Pharmaceutical Co Ltd

A process for preparing a gut glucagon antigen composed of a peptide-carrier complex is dis- closed, which comprises using as hapten a pep- tide represented by the following general formula; See Patent for Tabular Presentation PS wherein R represents a hydrogen atom, an amino acid group or a peptide group containing 2 to 20 amino acid groups, and reacting it with a carrier in the presence of a binding agent for bi- nding the hapten and the carrier to each other. Also, a process for preparing a gut glucagon antibody, which comprises administering the antigen described to mammals, and collecting the thus-produced antibody, is disclosed.

4407949

M E N I N G I T I S V A C C I N E

Peter J Kniskern, Arp Hagopian, Dennis Carlo assigned to Merck & Co [nc

A high molecular weight capsular poly- saccharide from H. influenza type b is an effec- tive immunogenic agent ap ins t infection caused by the H. influenza t ~ e b bacterium.