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Syllabus Committee Coordinator – Dr. Supriya K. Deshpande Semester I Paper I: Non-chordates Convener: Dr. Manisha Kulkarni Members: Prof. Dr. Rahul Jadhav, Dr. Utkarsha Chavan, Dr. Shital Taware Paper II: Developmental Biology I Convener: Dr. Roshan D’Souza Members: Dr. Sharad Mahajan, Dr. Vinayak Parab, Ms. Sandhya Kadiru Paper III: Genetics and Evolution Convener: Dr. Varsha Andhare Members: Dr. Surekha Gupta, Dr. Nandita Singh, Dr. Vinda Manjramkar Paper IV: Frontiers in Zoology Convener: Dr. Rajendra More Members: Dr. Pratiksha Savant, Dr. Mangesh Jamble, Dr. S.S. Waghmode Semester II Paper I: Chordates Convener: Dr. Dilip Kakvipure Members: Dr. Alka Chougule, Dr. Sushant Mane, Dr. Sandip Garg Paper II: Developmental Biology II Convener: Dr. Bhavita Chavan Members: Dr. Dandavate, Dr. Sashibhal Pandey, Dr. Rana Ansariya Paper III: Biochemistry and Biotechnology Convener: Dr. Subhash Donde Members: Dr. Rupinder Kaur, Dr. Rishikesh S. Dalvi, Dr. Gayathri N. Paper IV: Research Methodology Convener: Ms. Seema Ajbani Members: Nitin Wasnik, Dr. Minakshi Gurav, Dr. Devdatta Lad

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CONTENTS

1. Preface

2. Preamble

3. Pedagogy

4. Tables of Courses, Topics, Credits and Workload

5. Theory Syllabus for Semester I (Course codes: PSZO101-PSZO104)

6. Practical Syllabus for Semester I (Course code: PSZOP101-PSZO104)

7. References Course codes: (PSZO101- PSZO104)

8. Theory Syllabus for Semester II (Course codes: PSZO201-PSZO204)

9. Practical Syllabus for Semester II (Course code: PSZOP201-

PSZO204)

10. References (Course codes: PSZO201-PSZO204)

11. Marking Scheme of Examination (Theory and Practical)

12. Skeleton Papers: Semester I and Semester II

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PREFACE

The overall objective of revising the syllabus of Zoology, M.Sc. semester I and II is to offer students the advancements in the different components of the subject. The syllabus is framed and designed to include the recent developments useful for the students to shape their career. The purpose of revision of the syllabus is to upkeep the student with the necessary theory and practical, so that the learner can progress with confidence for his further independent fields into specialized areas such as research. The syllabus also embraces objectives and desired outcomes which will provide clear idea to the teacher as well as learner about the expected depth of the topic. The pedagogy supports a self-motivated learner to gain knowledge of the subject and prolong the borders of a classroom. The pedagogy recommends projects, assignments, visits etc. which is an extension to the regular classroom methodologies. It deals with the additional suggestions for bringing the objectives into actual instructional activities. The suggested resources in the reference lists would be useful in achieving the desired outcome. It would also assist an instructor in planning the lectures in a sensible modus beneficial for the learners. This syllabus was developed by the curriculum committee of 33 dedicated teachers. Their major role was to abridge, grow, augment and give a forward bearing to the Zoology taught in undergraduate curriculum, with projections to future requirements. They have successfully completed the colossal task. I congratulate them. – Dr. Deelip L. Bharmal Chairman BOS, Zoology

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PREAMBLE There is a vast ocean of information in various forms in today’s world of information technology. Recent research all over the world is incessantly adding and replacing earlier philosophies. To convert the scattered data in to the useful and applicable knowledge of the subject for the learners, we update our syllabus from time to time. The aim to revise the Zoology M.Sc. semester I and II syllabus is to equip and update our learners with most recent up gradations in the field and to develop useful skills in them to face the challenges of their future career. The present syllabus is prepared to provide most recent topics useful for the postgraduate students, keeping in mind their employability as well. The syllabus will also be expedient for the students aspiring for civil services. The whole team of thirty two dedicated members of the syllabus committee operated in unison and worked really hard to give out their best for the syllabus after several conventions, discussions, deliberations, explorations and exchange of innovative ideas. I acknowledge their efforts. There is always a scope for improvement in any work. So I request all stakeholders to give their inputs, valuable suggestions and recommendations to improve the syllabus during further revision. Coordinator Dr. Supriya K. Deshpande

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PEDAGOGY PSZO101 is ‘Non-chordates’, a study of Comparative functional anatomy, paleontology and phylogeny as well as all internal structures and various systems of non-chordates like protostomes and deuterostomes. It also assists in understanding of the anatomical similarities and differences of various systems. Practical aspects of functional anatomy and phylogeny could be undertaken through observation of various systems at laboratory level, field visits, constructing phylogenetic tree and making fossil casts. PSZO102 consists of ‘Developmental biology I’ which explains reproductive biology, molecular biology, genetics and morphology of non-chordates. The basic concepts of the reproductive process – fertilization could be taught considering the mechanism in relation to its molecular aspect. Applications of reproductive biology could be dealt with by taking various examples of economic importance. Practical aspects of developmental biology could be undertaken through observation of life cycles of various organisms that can be cultured in the laboratory as per the ethical permission granted so that learners can understand the theoretical approach in a much better way. The course PSZO103 ‘Genetics and Evolution’ will help the learner for conceptual understanding of the basic genetic principles along with genetic evolution and the impact it creates at the genome and molecular level with major phenotypic variation. Short basic experiments on drosophila or nematodes or video clips can be shown by the concerned teacher while clearing the concept of the topics. Teachers are expected to show the video clips or fossil photographs for the understanding of the topic. A visit to museum, institute or the archeological site to study fossils can be organised. The course PSZO104 ‘Frontiers in Zoology’ is prepared to introduce emerging interdisciplinary scientific fields such as Astrobiology and Chronobiology. For understanding of medical imaging techniques visits to medical diagnostic centers should be organized. Teachers are expected to elucidate the scope of medical diagnostic techniques as career options. Guest lectures of the experts could be arranged, who can provide additional insights to these aspects. Documentaries and videos based on climate change and global warming can be effectively used to provide in-depth knowledge to the learners. Case studies could be supplemented to understand the intensity of climatic change and global warming and its impact. The learners can be made aware about emotions, stress and associated health problems through the interaction with clinical psychologist and through videos of experts dealing the subject. In course PSZO201 ‘Chordates’, an attempt has been made to inform the students the basic comparative, functional morphology and phylogeny of chordates. Learners will be able to acquire the knowledge of evolutionary progression of primitive nonchordate to protochordates. They will also learn basic taxonomy of chordates, the beautiful world of biodiversity and through this they can enter in to the field of nature conservation or strategize to spread knowledge for future generations. This endeavor will be possible by utilizing various teaching methodologies such as use of model, charts and available specimens. This goal will to be achieved by the use of audio visuals, virtual dissections and observation of animals in their natural habitat through field visits. In course PSZO202 ‘Developmental Biology II’, generalized account of reproductive systems in chordates has been included to provide knowledge of reproductive system .This can be

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taught through virtual dissection. It also deals with physiology of reproduction where documentaries and videos can be shown to students for effective learning. Students can be given micro projects for in-depth knowledge or a visit to reproductive research centres could be organized. It can also be taught through case study and guest lectures. PSZO203 ‘Biochemistry and Biotechnology’ unit comprehends chemical and physicochemical processes which occur within living cell or organisms and utilization of biological processes for industrial and other purposes through the genetic manipulation of microorganisms respectively. The unit can be made more explicable by observing available video clips and visits on open day programs to the institutions like CIFE, NIRRH, ACTREC, KEM etc. The learners can also achieve an additional knowledge on the topic through attending free on- line courses on the https://www.coursera.org PSZO204 ‘Research methodology’ unit is framed to help the learners build a strong foundation for pursuing research. Visit to relevant websites should be undertaken to make them aware of different avenues for acquiring research grants, scholarships and funding and also to gain valuable insights about various model organisms and their applications in research. Methods of data management and analysis can be explained using different online and offline softwares to enable the learners to acquire these skill sets. The practical should be organized such that learners can be trained to manage large data sets generated via multiple observations, arrange them in a proper format and present them in relevant graphs/charts by adopting a hands-on in silico approach. Visits may be organized to nearby research institutions having CPCSEA certified Animal House and labs housing various model organisms so that students can acquire an in-depth knowledge on these topics. Guest lectures by leading research scientists may also be arranged to enrich the learners and stimulate their interest in forging a career in this field in the future.

– Conveners and committee members

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M. Sc. I Zoology Syllabus Choice Based Credit System

To be implemented from the Academic Year 2019-2020 SEMESTER I

Paper 1: Non-Chordates

Course Code UNIT Topic Headings Credits L/Week

PSZO101

1 Digestion and Excretion

4

1

2 Respiration and Circulation 1

3 Nervous System & Chemical Co-ordination and Reproductive System

1

4 Paleontology and Phylogeny 1

Paper 2: Developmental Biology I

Course Code UNIT Topic Headings Credits L/Week

PSZO102

1 Fertilization and Early Development in Non-chordates

4

1

2 Basic Concepts of Embryonic Development in Non-chordates

1

3 Early Development in Non-chordates 1

4 Special Aspects in Reproduction and Development in Non-chordates

1

Paper 3: Genetics and Evolution

Course Code UNIT Topic Headings Credits L/Week

PSZO103

1 Introduction to Evolutionary Genetics 4

1

2 Molecular Genetics 1

3 Evolution – Anthropology 1

4 Evolution and Fossils 1

Paper 4: Frontiers in Zoology

Course Code UNIT Topic Headings Credits L/Week

PSZO104

1 Astrobiology and Chronobiology 4

1

2 Modern Techniques in Medical Field 1

3 Biological Psychology and Neurobiology of Stress and Emotions

1

4 Climate Change and Sustainability 1

16 16

Practical

PSZOP101 Non-Chordates 2 1

PSZOP102 Developmental Biology I 2 1

PSZOP103 Genetics and Evolution 2 1

PSZOP104 Frontiers in Zoology 2 1

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SEMESTER II

Paper 1: Chordates

Course Code UNIT Topic Headings Credits L/Week

PSZO201

1 Protochordates and Agnatha

4

1

2 Phylogeny of Chordates 1

3 Functional and Comparative Anatomy of Chordates

1

4 Assorted Topics on Chordates 1

Paper 2: Developmental Biology II

Course Code UNIT Topic Headings Credits L/Week

PSZO202

1 Generalized Account of Reproductive Systems in Chordates

4

1

2 Physiology of Reproduction 1

3 Developmental Biology- Germ cells, Stem cells and Induced Breeding

1

4 Animal Development – Impact of Environment and Evolution

1

Paper 3: Biochemistry and Biotechnology

Course Code UNIT Topic Headings Credits L/Week

PSZO203

1 Fundamentals of Biochemistry 4

1

2 Metabolism and Metabolic Pathways 1

3 Processes and Techniques in Biotechnology

1

4 Applications of Biotechnology 1

Paper 4: Research Methodology

Course Code UNIT Topic Headings Credits L/Week

PSZO204

1 Principles of Scientific Research 4

1

2 Data Management and Analysis 1

3 Scientific Communication 1

4 Model organisms and their applications

1

16 16

Practical

PSZOP201 Chordates 2 1

PSZOP202 Developmental Biology II 2 1

PSZOP203 Biochemistry and Biotechnology 2 1

PSZOP204 Research Methodology 2 1

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M. Sc. Part 1: Semester I (Theory) Paper 1: PSZO101: Non-Chordates

Unit 1: Digestion and Excretion (15L) Objectives

To understand functional anatomy, differences and similarities of digestive and excretory systems of non-chordates.

Desired Outcome

The learner will be able to differentiate anatomical and physiological modifications of digestive and excretory systems of non-chordates like Protostomes and Deuterostomes.

1.1 General organization of digestive systems of Protostomes (Annelids, Arthropods

and Molluscs) and Deuterostomes (Echinoderms and Hemichordates)

1.2 Digestive Systems with Special emphasis on the organs or glands of : 1.2.1 Annelids

Clamworm – Denticles or Paragnaths, Jaws 1.2.2 Arthropods

Prawn: Buccal cavity, Cardiac and Pyloric stomach, Hepatopancreas

1.2.3 Molluscs Pila: Odontophore, Sub-radular organ, Radula, Oesophageal pouches, Cardiac

and Pyloric chambers, Salivary gland, Hepatopancreas 1.2.4 Echinoderms

Starfish: Cardiac and Pyloric stomach, Intestinal Caeca, Digestive glands 1.2.5 Hemichordates

Balanoglossus: Buccal Diverticulum, Digestive Pharynx, Post-branchial canal, Hepatic and post-hepatic regions

1.3 General organization of excretory systems of Protostomes (Annelids, Arthropods and Molluscs) and Deuterostomes (Echinoderms and Hemichordates) 1.4 Excretory Systems with Special emphasis on the organs/structures or glands of:

1.4.1 Annelids Clamworm: Coiled Nephridia

1.4.2 Arthropods Prawn: Antennal glands or Green glands or Maxillary glands, Bladder, Renal

sac

1.4.3 Molluscs Pila: Organ of Bojanus, anterior Renal chamber, posterior Renal chamber

1.4.4 Echinoderms Starfish: Water Vascular System, Coelomocytes, Intestinal caeca

1.4.5 Hemichordates Balanoglossus: Glomerulus or Proboscis gland

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Unit 2: Respiration and Circulation (15L) Objectives

To understand anatomical differences and similarities of respiratory and circulatory systems in non-chordates.

To understand physiological processes in non-chordates. Desired Outcome

The learner will be able to differentiate anatomical and physiological modification in respiratory and circulatory systems of non-chordates like Protostomes and Deuterostomes.

2.1 General organization of respiratory systems of Protostomes (Annelids, Arthropods and Molluscs) and Deuterostomes (Echinoderms and Hemichordates) 2.2 Respiratory Systems with Special emphasis on the organs/structures or glands of:

2.2.1 Annelids Clamworm: Parapodia, Body wall

2.2.2 Arthropods Prawn: Branchiostegites, Branchiae, Epipodite

2.2.3 Molluscs Pila: Ctenidium, Pulmonary sac

2.2.4 Echinoderms Starfish: Dermal papulae, Tube Feet

2.2.5 Hemichordates Balanoglossus: Gill slits, Branchial sac, Gill pores

2.3 General organization of circulatory systems of Protostomes (Annelids, Arthropods and Molluscs) and Deuterostomes (Echinoderms and Hemichordates) 2.4 Circulatory systems with special emphasis on the organs/ structures or glands of:

2.4.1 Annelids Clamworm: Dorsal blood vessel, Ventral blood vessel, Transverse vessels

2.4.2 Arthropods Prawn: Heart and Blood lacunae

2.4.3 Molluscs Pila: Pericardium, Heart

2.4.4 Echinoderms Starfish: Perihaemal sinuses, Axial gland

2.4.5 Hemichordates Balanoglossus: Central Sinus (Heart), Dorsal vessel, Ventral vessel

Unit 3: Nervous System, Chemical Co-ordination and Reproductive System (15L) Objectives

To compare nervous systems and chemical co-ordination in non-chordates.

To understand similarities and differences in the reproductive systems of non-chordates.

Desired Outcome

The learner will be able to differentiate anatomical and physiological modifications of nervous systems and chemical co-ordination of non-chordates.

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The learner will be able to differentiate modifications of reproductive systems of non-chordates.

3.1 General organization of Nervous systems& Chemical Co-ordination in

Protostomes (Annelids, Arthropods and Molluscs) and Deuterostomes (Echinoderms and Hemichordates)

3.2 Nervous system & Chemical Co-ordination with special emphasis on the organs/structures or glands of:

3.2.1 Annelids Clamworm: Corpora pedunculata, Giant fibre, Circumpharyngeal ganglion,

Peristomal cirri, Nuchal organ, pigmented Retinal cells 3.2.2 Arthropods

Prawn: Brain, Circum-oesophageal commissure, Compound eyes,

Chromatophore, Statocysts, Tactile setae, Olfactory setae, Tangoreceptors,

Chemoreceptors, Proprioreceptors, Sinus gland X organ complex, Y organ

3.2.3 Molluscs Pila: Cerebral Ganglia, Buccal Ganglia, Pleuro-pedal Ganglionic Mass, Supra-

intestinal Ganglion, Visceral Ganglion, Osphradium, Tentacles, Statocysts, Eyes, Mechanoreceptors, Photoreceptores, Temperature receptors, Chemoreceptors

3.2.4 Echinoderms Starfish: Ectoneural nervous system, Langer’s nerve, Eyes, Terminal tentacles,

Neurosecretory cells 3.2.5 Hemichordates

Balanoglossus: Epidermal Plexus, Preoral ciliary organ, Neurosecretory cells 3.3 General organization of reproductive systems in Protostomes (Annelids, Arthropods and Molluscs) and Deuterstomes (Echinoderms and Hemichordates) 3.4 Reproductive systems with special emphasis on the organs/structures or glands in:

3.4.1 Annelids Clam worm: Gonads – Testes and Ovaries (Temporary)

3.4.2 Arthropods Prawn: Male – Testes, Vasa deferentia, Vasiculae seminalis; Female – Ovaries,

Oviducts

3.4.3 Molluscs Pila: Male – Testes, Vasa efferentia, Vasa deferens, Vasicula seminalis,

Hypobranchial glands; Female – Ovary, Oviduct, Receptaculum seminis,

Uterus, Vagina, Hypobranchial gland

3.4.4 Echinoderms Starfish: Male / Female gonads

3.4.5 Hemichordates Balanoglossus: Saccular gonads

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Unit 4: Paleontology and Phylogeny (15L) Objectives

To understand evolution of non-chordates by studying their fossils found in Indian sub-continent.

To understand phylogeny of non-chordates. Desired Outcome

The learner will be able to understand the evolution of non-chordates and their phylogenetic relationships by means of paleontological evidences.

4.1 Fossils of:

4.1.1 Annelids: Serpula and Glomerula spp. found in South India 4.1.2 Arthropods: Trilobites found in Kashmir, Insects trapped in amber found in Western India 4.1.3 Molluscs: Molluscs fossils found in Kutchh, Chhattisgarh and Jammu – Palmerella kutchensis, Cimomia forbesi, Lamellidens 4.1.4 Echinodermata: Gongrochanus spp. found in South India, Echnoid fossils found in India. 4.1.5 Hemichordata: Graptolite fossils

4.2 Phylogenetic affinities of Annelida, Arthropoda, Mollusca, Echinodermata and Hemichordata

4.2.1 Phylogenetic relationships of Trochophore larva 4.2.2 Similarities and differences between Annelids and Molluscs 4.2.3 Phylogeny of Arthropods – Monophyletic theory and polyphyletic theory 4.2.4 Phylogeny of Echinoderms – Asterozoan hypothesis and Cryptosyringid hypothesis 4.2.5 Affinities of Annelida with Hemichordata 4.2.6 Affinities of Echinodermata with Hemichordata

4.3 Construction of Phylogenetic Tree

4.3.1 Genetic distance 4.3.2 Rooting trees

4.4 Phylogenetic Tree Building Methods

4.4.1 Distance-Based Methods: o UPGMA Method o Neighbour Joining Method (NJ) o Weighted Neighbour-Joining (Weighbour) o Fitch-Margoliash (FM) and Minimum Evolution (ME) Methods

4.4.2 Character-based Methods: o Maximum parsimony (MP) o Maximum Likelihood (ML)

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Paper 2: PSZO102: Developmental Biology – I UNIT 1 – Fertilization and Early Development in Non-chordates (15 L) Objectives

To introduce learners to the concept, mechanism and molecular events of fertilization in non-chordates

To introduce the concept of germ layers and coelom formation.

Desired Outcome

The learner will understand the mechanism of fertilization and its molecular events in non-chordates.

The learner will appreciate the process of formation of germ layers and coelom in animals and understand the difference in these processes between Protostomes and Deuterostomes.

1.1 Concept and Mechanism of Fertilization

1.1.1 Types of reproduction – Asexual and sexual 1.1.2 Fertilization – Concept and types 1.1.3 Mechanism of fertilization

Capacitation of sperm Recognition of sperm and egg Acrosome reaction Activation of egg Fusion of egg and sperm cell membrane Amphimixis

1.2 Molecular Events of Fertilization

1.2.1 Species specific recognition of gametes – Fertilizin and antifertilizin interaction, Binding protein

1.2.2 Prevention of polyspermy – Fast and slow block 1.2.3 Calcium as initiator of cortical granule reaction 1.2.4 Activation of egg metabolism with respect to Sea urchin

1.3 Formation of Germ Layers and Coelom Formation

1.3.1 Post Fertilization events: Overall view of Cleavage, Blastulation, Gastrulation 1.3.2 Formation of Germ layers – Diploblastic, Triploblastic 1.3.3 Coelom formation in Protostomes and Deuterostomes

Unit 2: Basic concepts of embryonic development in non-chordates (15L) Objectives:

To introduce basic concepts of embryonic development and embryogenesis.

To understand therapeutic applications of stem cells. Desired outcome:

Learners would understand basic concepts and aspects of embryogenesis and stem cell therapy.

2.1 Fate map and cell lineages: potency and commitment

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2.2 Competence and induction 2.3 Mosaic and regulative development 2.4 Cell specification:

2.4.1 Autonomous specification 2.4.2 Conditional specification 2.4.3 Syncytial specification

Unit 3: Early Development in non-chordates (15L) Objectives:

To introduce the learners about the process of early development and differences in the pattern of development in non-chordates.

To help learners understand specific gene regulation during development.

Desired Outcome

Learners will understand the mechanism of early development and able to correlate the various differences observed in the pattern of embryonic development in non-chordates.

Learners will understand the role of certain genes in early development 3.1 Development of Caenorhabditis elegans

3.1.1 Pattern of cleavage 3.1.2 Anterior-posterior axis formation 3.1.3 Dorsal-Ventral axis formation 3.1.4 Cell specification (Autonomous and Conditional) 3.1.5 Process of gastrulation

3.2 Early Development in Drosophila melanogaster:

3.2.1 Pattern of cleavage 3.2.2 Blastulation 3.2.3 Process of gastrulation 3.2.4 Anterior-posterior pattern formation by morphogenetic protein gradient 3.2.5 Dorsal-ventral pattern formation by morphogenetic protein gradient 3.2.6 Patterns of homeotic gene expression: Homeo selector gene complexes (Hom-C, homeotic gene complex) – Antennapedia and bithorax complexes and their functional domains, Realistor genes: distal less and wingless genes

Unit 4: Special Aspects in Reproduction and Development in non-chordates (15L) Objectives:

To understand diversities in reproduction and development in non-chordates.

To understand the mechanism of regeneration

To help learners to relate applications of developmental biology with forensics and IPM Desired outcome:

Learners will acquire knowledge about the diversities in reproduction and development in invertebrates and the process of regeneration in lower animals.

Learners will understand how principles of developmental biology can be applied in forensics and IPM

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4.1 Sexual Reproduction in Protozoa

Conjugation in Paramoecium sp. Syngamy in flagellates

4.2 Metagenesis in Obelia sp. 4.3 Polyembryony in Fasciola sp. 4.4 Regeneration in Hydra (Morphallactic) and Planaria (Totipotency) 4.5 Parthenogenesis in Aphids 4.6 Metamorphosis in insects – Indirect Development

4.6.1 Types of insect metamorphosis 4.6.2 Eversion and differentiation of imaginal disc 4.6.3 Hormonal control of insect metamorphosis 4.6.4 Study of life cycle of Blow fly-applications in forensics 4.6.5 Larval forms of non-chordates: Amphiblastula larva (Porifera), Planula

(Coelenterata), Echinopluteus (Echinodermata) and Tornaria (Hemichordata) 4.7 Pheromones in invertebrates and their role as sex attractants and in Integrated Pest Management (IPM)

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Paper 3: PSZO103: Genetics and Evolution Unit 1: Introduction to evolutionary genetics: (15L) Objectives:

To develop in learners the understanding of genes, genomes in evolution.

To comprehend the pattern of genetic relatedness between all species.

To understand the contribution of genetic variation in phenotypic expression Desired outcome:

The learners will understand the genetic analysis at the gene, genome and population level.

The learner would realize the flow of genetic information and complex networking of genes in biological system leading to major phenotypic changes.

1.1 Cytochrome ‘c’

1.1.1 Structure of Cytochrome ‘c’ and couple cytochrome ‘c’ 1.1.2 Cytochrome ‘c’ in eukaryotes. Example Primates 1.1.3 Cox gene 1.1.4 Molecular basis of haemoglobin gene structure. Haemoglobin as a model of

evolution 1.2 Sex Determination and Evolution of Sex Chromosome

1.2.1 Theory of evolution of sex chromosome 1.2.2 Evolution of sex chromosomes from autosomes 1.2.3 Biology of Y chromosome 1.2.4 Molecular level of sex determination in mammals 1.2.5 Sex based gene expression 1.2.6 T6sRNA

1.3 Cytoplasmic Inheritance/Extra-nuclear Inheritance

1.3.1 Introduction – human mitochondrial genetics 1.3.2 Paternal and maternal mtDNA inheritance in humans 1.3.3 mtDNA mutations and human diseases 1.3.4 Salient feature of cytoplasmic inheritance

1.4 Selfish Gene 1.4.1 Introduction and definition 1.4.2 Individual altruism and genetic egoism 1.4.3 Green beard effect

Unit 2: Molecular Genetics (15L) Objectives:

To get acquainted with applications of genes in modern science and population genetics.

To give in-depth knowledge of biological processes through molecular mechanisms. Desired Outcome:

The learner will understand the molecular processes that occur in and between the cells.

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The learner will gain insight in most significant molecular and cell based methods used to expand the understanding of modern Biology.

2.1 Gene Library

2.1.1 Molecular analysis of gene and gene products 2.1.2 Types – Genomic Library, cDNA Library 2.1.3 Construction of genomic library – human antibody gene library 2.1.4 Applications of gene library

2.2 Types of Genes: Definition

Multigenes Repeated genes Single copy gene House-keeping gene (constitutive gene) Luxury gene (non-constitutive gene) Pseudogene Jumping gene (Transposons) Overlapping gene Structural gene Processed gene Split gene Regulatory gene

2.2.1 Multi-gene Families and Types:

a) Split Genes or Interrupted genes

Introduction

Structure of split genes

Discovery and theory of split genes

Evolution of split genes b) Pseudogenes c) Selfish gene

2.2.2 Overlapping Gene 2.3 Mapping units

2.3.1 Types –genetic mapping and physical mapping 2.3.2 Detection of linkages 2.3.3 Construction of linkage maps in diploids and their characteristics 2.3.4 Co-efficient of coincidence 2.3.5 Outline of other types of gene mapping

2.4 Gene cloning

2.4.1 Definition and strategies of gene cloning 2.4.2 Techniques in gene cloning 2.4.3 Applications of gene cloning in DNA analysis in research

2.5 Population genetics 2.5.1 Population genetics in DNA typing 2.5.2 Mutation – Selection balance 2.5.3 Parallelism between random drift and inbreeding

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Unit 3: Evolution – Anthropology (15L) Objective:

To give an insight to students about the expression, perpetuation and adaptations of animals in varied environment during the process of evolution

Desired Outcome:

Learner will be able to gain knowledge of altruism, co-evolution and the racial distribution of animals in evolutionary time scale

3.1 Racial distribution of Primates and Homo sapiens

3.1.1 Racial distribution of primates Distribution of Prosimians (Lemurs, Lorises, Galagos) Distribution of Anthropoids (Monkeys and Apes)

3.1.2 Divergence of Homo sapiens from Anthropoid 3.2 Racial distribution of Homo sapiens

3.2.1 Biological races in humans Caucasoid (White) race Negroid (Black) race Capoid (Bushmen/Hottentots) race Mongoloid (Oriental / Amerindian) race Australoid (Australian Aborigine and Papuan) race

3.2.2 Human genetic diversity protein-based polymorphism (Blood groups, Alu polymorphism), craniometrics

3.3Importance of Human Genetic Biodiversity

3.3.1 Applications – Anthropological, Medical, Cultural 3.3.2 Parasites and human co-evolution 3.3.3 Ectoparasite’s co-evolution with humans (Head Louse, Scabies, Mite) 3.3.4 Endoparasites co-evolution with humans (Liver fluke, Tapeworm, Ascaris, Sickle

cell Individuals as an example of co-evolution with malarial parasite) 3.4 Altruism and Evolution

3.4.1 Altruism – Intra-species and Interspecies, Cooperation and its evolution in human 3.4.2 Altruism and evolution in domesticated wild animals 3.4.3 Group selection and kin selection 3.4.4 Domestication and behavioral changes

3.5 Hybrid Evolution

3.5.1 Natural Hybrids – Origin and evolution of animal hybrids, Insects, Snails, Fish, Frog, Lizard

3.5.2 Hybrid Speciation 3.5.3 Artificial Hybrids – Hybrid optimization – Bee Colony 3.5.4 Artificial cells in hybrids to create

Dzo Mule Hinny

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Liger Wholpin Leopon Zorse

Unit 4: Evolution & Fossils (15L) Objectives:

To help students to solve biological problems that impact our lives and to understand similarities and differences between human and other species with respect to body form, behaviour, adaptation, distribution pattern and physiology.

To understand learner that fossil records provide history of earth as they occur in a particular order.

To impart the learner with knowledge of preserving endangered species by the technique of biomaterial banking, assisted reproductive technology and frozen zoo.

Desired Outcome:

The learner will be able to apply evolutionary principles to research and understand aspects of evolution.

Learner will be acquainted with techniques of dating fossils and formation of fossils.

The learner will understand the concept of frozen zoo and will foster the need of hour for conservation of endangered species.

4.1 Human evolution

4.1.1 Human evolutionary history and placing humans on tree of life 4.1.2 Genomics and humanness; current issues in human evolution 4.1.3 Brief Accounts of Parapithecus spp., Propliopithecus spp., Dryopithecus spp.,

Ramapithecus spp., Australopithecus spp., Kenyanthropus spp., Homo spp., Neanderthal, Cromagnon and Modern man

4.1.4 Concept of neutral evolution, molecular divergence and molecular clock 4.2 Fossil dating

4.2.1 Geological timescale – Eras, Periods and Epochs 4.2.2 Major events in evolutionary time scale 4.2.3 Significance of fossils and fossilization 4.2.4 Fossil dating – Types: Absolute and Relative Dating 4.2.5 Process of C-14 Carbon dating

4.3 Frozen zoo

4.3.1 Concept of frozen zoo to save endangered species 4.3.2 Techniques to create offspring from cells of endangered species, nuclear transfer,

mixing cells, creating sperms and eggs 4.3.3 Current status of frozen zoo

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Paper 4: PSZO104: Frontiers in Zoology Unit 1: Astrobiology and Chronobiology (15L) Objectives:

To introduce recent interdisciplinary scientific fields to the learners.

To inculcate interest for interdisciplinary research among the learners. Desired outcome:

Learners will get the introductory knowledge of emerging areas correlated with Zoology.

Learners will understand the different types of biological clocks and their importance.

1.1 General aspects of Astrobiology, status of research in astrobiology and future perspectives, factors determining habitability on planets; extremophiles 1.2 Bio-signatures of life in exoplanet atmosphere 1.3 Search for life on Mars; search for extra-terrestrial intelligence 1.4 Introduction to Chronobiology – Definition and meaning 1.5 Biological rhythms

1.5.1 Introduction 1.5.2 Types

Circadian rhythms Tidal rhythms Lunar rhythms Semilunar rhythms Circannual rhythms

1.6 Structure of Biological Clock in Insects and Vertebrates; biological clock function 1.7 Relevance of Biological Clocks for Human Welfare

1.7.1 Chronomedicine 1.7.2 Chronotherapy

1.8 Molecular mechanisms controlling the circadian rhythm (Noble Prize winning work, 2017) Unit 2: Modern Techniques in Medical Field (15L) Objectives:

To provide the basic knowledge of different modern techniques used in medical field.

To provide information of applications of biomedical techniques. Desired outcome:

Leaner would be able to explain the basic principles, applications of the major imaging and DNA bar coding techniques.

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2.1 Physical aspects of medical imaging 2.1.1 Laser beam in Biology and Medical field 2.1.2 Medical lasers (Carbon Dioxide Laser, Nd: YAG Laser) 2.1.3 Applications of lasers in therapy and diagnosis.

2.2 Principle and applications of major medical imaging techniques

2.2.1 Radiography 2.2.2 Echocardiography (2D and 3D Echo) 2.2.3 Computed Tomography Scan (CT scan) 2.2.4 Magnetic Resonance Imaging (MRI)

2.3 Radioisotope Imaging: Introduction and applications 2.4 DNA bar coding technique and its applications 2.5 Molecular Cytogenetic Techniques and their Applications

2.5.1 Fluorescence in situ Hybridization (FISH) 2.5.2 Genomic in situ Hybridization (GISH) 2.5.3 Flow cytometry 2.5.4 Automated karyotyping 2.5.5 Chromosome painting

Unit 3: Biological Psychology and Neurobiology of Stress and Emotion (15L) Objectives:

To give learners the basic knowledge of emotions, stress and associated health problems.

To introduce learners to the brain regions that forms the basis of emotions. Desired outcome:

Learner will get acquainted with brain regions that form the basis of emotions and stress.

Leaner will also understand the health problems caused due to prolong emotion and stress on the body.

3.1 Introduction and general features of emotions 3.2 Neuronal basis of emotion 3.3 Types of emotions – Definition and management

Fear Frustration Joy / Happiness Sadness Embarrassment Guilt Shame Anger Love Inspiration

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3.4 Measurement of emotions Self-report Behavioral observations Physiological measure

3.5 Role of brain regions in emotions

Limbic system Amygdala Cortex Hypothalamus Midbrain Periaqueductal Grey Neurochemicals involved in emotions

3.6 Concept of Stress, Measurement of stress, Characterising stress – Signs of stress, Types of stressors 3.7 Stress and the Hypothalamus-Pituitary-Adrenal Axis (HPA Axis) 3.8 Remedial measures to combat psychological stress and negative emotions Unit 4: Climate Change and Sustainability (15L) Objectives:

To provide the information about the causes of climatic change.

To make the student aware of various national and international initiatives on climate change.

Desired outcome:

The learner will understand current challenges of climatic changes and environmental issues.

Learner will understand the importance of the sustainable development. 4.1 Climate change

4.1.1 Introduction 4.1.2 Indicator species of climate change 4.1.3 Vulnerability and adaptations

4.2 Greenhouse effect

4.2.1 Greenhouse gases 4.2.2 Increase in greenhouse gas concentrations 4.2.3 Global warming potential of greenhouse gases

4.3 Intergovernmental Panel on Climate Change [IPCC]: Objectives and functioning 4.4 United Nations Framework Convention on Climate Change (UNFCCC) – Salient Features and Objectives

4.4.1 Kyoto protocol 4.4.2 Doha Amendment 4.4.3 Emission trading / Carbon Trading

4.5 Sustainable development

4.5.1 Introduction and goals

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4.5.2 Sustainable habitat – Green building, Griha rating norms 4.6 Green Audit – Introduction and Types:

Energy audit Waste disposal audit Water audit Carbon audit

4.7 Impact of climate change on terrestrial and aquatic organisms 4.8 Case Studies on Climatic Change and Global Warming

Kerala floods Impact of highway and road construction on animal survival Impact of urbanization: Dhaka – Bangladesh

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M. Sc. Part 1: Semester I Practical 1 PSZOP101: Non-Chordates

1. Study of digestive systems of clamworm, prawn, Pila. 2. Study of digestive systems of starfish, Balanoglossus. 3. Study of excretory systems of clamworm, prawn, Pila. 4. Study of excretory systems of starfish, Balanoglossus. 5. Study of larval forms of Annelids, Arthropods, Molluscs with reference to phylogeny. 6. Study of larval forms of Echinoderms with reference to phylogeny. 7. Study of nervous systems of clamworm, prawn, Pila. 8. Study of nervous systems of starfish and Balanoglossus. 9. Study of male reproductive systems of clamworm, prawn, Pila. 10. Study of male reproductive systems starfish, Balanoglossus. 11. Study of female reproductive systems of clamworm, prawn, Pila. 12. Study of female reproductive systems starfish, Balanoglossus. 13. A. Study of Fossils:

a. Annelids: Serpula and Glomerula spp. b. Arthropods: Trilobites, Insects trapped in amber c. Molluscs: Palmerella kutchensis, Cimomia forbesi, Lamellidens

B. Study of Fossils: d. Echinodermata: Gongrochanus spp., Goniocidaris e. Hemichordata: Graptolite fossils

14. Construction of phylogenetic tree. 15. Making of fossil casts with Shaadu Clay / Muddy soil*. 16. Field visit to observe invertebrates in their natural habitat and accordingly the

modifications of various systems. 17. Field visit to study fossils to the places such as museums, Institutions, quarries,

archeological sites etc. *Note: Plaster of Paris and Plasticine Modelling clay should not be used to make fossil casts.

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Practical 2: PSZOP102: Developmental Biology – I

1. To culture Paramecium to study conjugation and binary fission under the microscope. 2. To observe development of C. elegans. 3. To culture Drosophila to study its life cycle. 4. To observe stages of Tribolium or Sitophilus to understand indirect development in

animals. 5. To study germ layers:

Diploblastic – T.S. of body wall of sponges and cnidarians (coelenterates)

Triploblastic acoelomate – T.S. of body wall of Platyhelminthes

Triploblastic pesudocoelomate– T.S. of body wall of Ascaris

Triploblastic coelomate –T. S. of body wall of earthworm 6. To study gemmule in sponge. 7. To study larvae of non-chordates:

Porifera – Amhiblastula

Cnidaria (Coelenterata) – Planula

Annelida and Molluscs– Trochophore

Mollusca – Glochidium

Crustacea o Nauplius o Zoea o Mysis o Megalopa

Echinodermata o Auricularia o Echiopluteus

Hemichordata– Tornaria 8. To study Life cycle of Butterfly / Moth (Insecta).

Observation should be done by using Permanent slides/ Photos/Charts/ Preserved specimens/Animal models for studying the developmental aspects.

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Practical 3: PSZOP103: Genetics and Evolution 1. To Isolate DNA from the given blood sample.

2. To study the Kappa particles in Paramecium cultured in laboratory.

3. To study the maternal effects in Drosophila due to induced environmental changes.

4. To study the Avian brood parasitism in cuckoo, crow, Asian koel, Shiny cowbird.

5. To study Haemoglobin of domesticated dogs, cats, cattles with the help of comparative

protein sequence charts using bioinformatics tools.

6. Study of Altrusim, reciprocal altruism in Vampire bat, thumping of ground gene in Rabbit.

7. To study different types of fossils:

a. Body Fossils – A wall of large dinosaur bones, Turritella snail shells from the Miocene age (Photograph) b. Mould & casts – Ammonite, Clam shell c. Per mineralization or petrifaction fossils – Per mineralized Dinosaur Bone (Photograph), permineralized Fern Stripe (Photograph).

8. To study cloning in India – eg. Garima – Assamese Buffalo, Noori – Pashmina goat. 9. Study of syndromes – Cockeye syndrome (CS), Proteus syndrome, Muenke syndrome. 10. Problems on gene mapping and mitotic index.

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Practical 4: PSZOP104: Frontiers in Zoology 1. Identification of photographs with respect to chronobiology.

a. Structure of biological clock in insects b. Structure of biological clock in vertebrates

2. Identification of photographs of different imaging techniques. 3. Determination of concentration of iron as a pollutant from sample / sample solution by –

Redox titration or spectrophotometric method. 4. Detection of heavy metals from given sample: a) Zinc b) Lead c) Copper. 5. To estimate the amount of dust (particulate matter) deposition on the leaves of roadside

plants. 6. Study of animals as indicators for climate change and global warming

a. Green turtle b. Birds – Sparrow c. Polar bear d. Coral reef e. Butterflies

7. Study of gross anatomical regions of brain involved in stress and emotion. 8. Environment Audit report (Green Audit / Review of EIA of a Selected Area) 9. Visit to nearby bar coding laboratory / imaging center and submission of report. 10. Assignment or report submission on pulse rate after stress.

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References PSZO101: 1. Invertebrates – Protozoa to Echinodermata, Author: Ashok Verma, Publisher- Narosa

Publishing House. 2. Invertebrate Zoology, Authors: E. L Jordan and P. S. Verma, Publisher: S. Chand and

Company Ltd. 3. Invertebrate Zoology 3rd edition, Author: Paul A. Meglitsch and Fredrick R. Schram,

Publisher – Oxford University Press. 4. Introduction to General Zoology Vol. I, Author: Korak Kanti Chaki, Gautam Kundu and

Supriti Sarkar, New Central Book Agency (P) Ltd. 5. Introduction to General Zoology Vol. II, Author: Korak Kanti Chaki, Gautam Kundu and

Supriti Sarkar, New Central Book Agency (P) Ltd. 6. Modern Text Book of Zoology: Invertebrates, Author: R. L. Kotpal. 7. Invertebrate Zoology by N.C Nair, S. Leelavathy, et al. 8. Textbook of Zoology: Invertebrates Vol I 7/e by Haswell. 9. Textbook of Invertebrate Zoology by Dev Bhattacharya. 10. Invertebrates by Richard C. Brusca, Wendy Moore, Stephen M. Shuster. 11. Invertebrate Zoology: A Functional Evolutionary Approach, Authors: Richard Fox, Robert

D. Barnes, Edward E. Ruppert. 12. An Introduction to Mollusca by G. S. Sandhu and Harshvardhan Bhaskar, Campus Book

International. 13. An Introduction to Echinodermata by G. S. Sandhu and Harshvardhan Bhaskar, Campus

Book International. 14. Principles of Invertebrate Paleontology by N. Shrock. 15. Palaeontology Invertebrate by Henry Woods. 16. Phylogenetic Trees Made Easy: A How-to Manual by Barry G. Hall. 17. Invertebrate Zoology by P. S. Dhami and J. K. Dhami. 18. Practical Zoology by K. C. Ghose 19. Practical Zoology – Invertebrate by S. S. Lal. 20. http://www.biologydiscussion.com/zoology/practicals/structure-of-squid-loligo-with-

diagram-zoology/60685 References PSZO102: 1. Developmental Biology – Scott F. Gilbert – 8th Edition, Sinauer Associates Inc., 2006 2. Principles of Development – L. Wolpert – 4th Edition, Oxford University Press, 2011. 3. Essential Developmental Biology- J.M.W. Slack – 2nd Edition, Blackwell Publishing, 2006 4. Modern Text Book of Zoology- Invertebrates by R.L .Kotpal, Rastogi Pub,10th Edn, 2013. 5. An Introduction to Embryology by B. I. Balinsky, CBS College Pub, 5th Edn, 1981. 6. Biology of the Invertebrates by Jan A. Pechenik, Tata McGraw- Hill, 4thEdn, 2000. 7. Invertebrate Zoology by P. S. Dhami and J. K. Dhami, R. Chand and Co.3rd

Rev.Edn,1974 8. General and Applied Entomology by B. V. David and T. N. Ananthakrishnan, Tata

McGraw- Hill, 2thEdn, 2004. 9. Modern Entomology by D. B. Tembhare, Himalaya Publishing House,1st Edn,1997. 10. https://phys.org/news/2014-09-blowfly-maggots-physical-evidence-forensic.html 11. https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3296382/ 12. https://www.canr.msu.edu/resources/pheromone_traps_the_gypsy_moth_e2585

References PSZO103: 1. Morphology of the primates and human evolution R.P Srivastava. 2. Animal behavior by S. Prasad.

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3. Text of Animal Behaviour by H.S Gundevia. 4. Animal behavior Saras publication Kenneth Oakley, Title of book. 5. Fossil in Earth Sciences by Anis kumar Ray. 6. Evolution Donal R. Prothero. 7. Principals of paleontology David M. Raup, Stanley. 8. Fossil Records, John Morris. 9. General genetics by Winchester. 10. Molecular Biology of gene by Watson et al. Vol I & II. 11. Genetics by Strickberger. 12. Molecular Biology by Friefielder. 13. Genetics by P.K. Gupta. 14. Molecular cell biology by Darnell, Lodish and Baltimore (Scientific American books). 15. Evolution by Savage. 16. Process of organic evolution by Stebbings. 17. Evolution of vertebrates by Colbert Concepts of Genetics, 9th edition (2008), William S.

Klug, Michael R. Cummings, Charlotte Spencer, and Michael A. Palladino, Publisher-Benjamin Cummings.

18. Genes IX, 9th edition (2008), Benjamin Lewin, Publisher-Jones and Barlett Publishers Inc. 19. Principles of Genetics, 4th edition, (2006), Snustad D. Peter and Simmons J. Micheal,

Publisher -John Wiley and Sons. Inc. 20. Genetics, (1999), Daniel J. Fairbanks, W. Ralph Andersen Publisher- Brooks/Cole Pub

Co. 21. Principles of Genetics, 8th edition (1991), Eldon J. Gardner, D.P. Snustad, M.J. Simmons,

and D. Peter Snustad Publisher-John Wiley and Sons. Inc. 22. Microbial Genetics, (1987), David Freifelder, Publisher-Jones & Bartlett. 23. General Genetics, (1985), Leon A. Snyder, David Freifelder, Daniel L. Hartl Publisher-

Jones and Bartlett. 24. Genetics, 3rd edition, Monroe W. Strickberger, (1968), Publisher – Macmillan Publishing

Co. References PSZO104: 1. Animal Behaviour (Ethology) by Dr. V. K. Agarwal, S. Chand and Company Ltd. New

Delhi. 2. Chronobiology Biological Timekeeping: Jay. C. Dunlap, Jennifer. J. Loros, Patricia J. De

Coursey (ed). 2004, Sinauer Associates, Inc. Publishers, Sunderland, MA, USA. 3. Insect Clocks, by D.S. Saunders, C.G.H. Steel, X., afopoulou (ed.) R.D. Lewis. (3rd Ed)

2002 Barens and Noble Inc. New York, USA. 4. Biological Rhythms: Vinod Kumar (ed.2002) Narosa Publishing House, Delhi/ Springer-

Verlag, Germany. 5. Molecular mechanisms controlling the circadian rhythm by Michael Rosbash, Michael W.

Young & Jeffrey C. Hall, (Nobel Prize winner 2017). 6. Principles and techniques of practical biochemistry Ed. B. L. Williams and amp; K.

Wilson, Arnold Publishers. 7. Medical Imaging Physics by W. R. Hendee, E.R. Ritenour, Wiley-Liss, 2002. 8. An Introduction to The Principles of Medical Imaging, Guy, D. ffytche, Imperial College

Press, 2008. 9. Medical Instrumentation applications and design by John G. Webster. 10. Biomedical Instrumentation Technology and applications by Khandpur. McGraw – Hill. 11. Biomedical Instrumentation systems by Shakti Chatterjee.

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12. Hand Book of Bio-Medical Instrumentation by R. S. Khandpur, McGraw Hill Publishing Co. Ltd. 2003.

13. Biomedical Instrumentation and Measurements Leslie Cromwell, Fred J. Weibell, Erich A. Pfeiffer, Pearson Education.

14. Bio-Medical Instrumentation by M. Arumugam, Anuradha Agencies 15. Lasers and Optical Fibers in Medicine by Katzir A.: Academic Press, Inc. 1993. 16. Techniques in life science by Tembhare. 17. Biological Psychology 3rd edition, by Frederick Toates, Prentice Hall, Pearson Education

Limited. 18. Biological Psychology,10th Edition, James V. Kalat, North Carolina State University, 2009,

2007 Wadsworth, Cengage Learning. 19. Introduction to Psychology, 9th edition, James Kalat North Carolina State University,

Wadsworth, Cengage Learning. 20. Neurobiology – Gorden M. Sheperd. 21. Principles of Neural Science – E. Kandel and others. 22. Essentials of Neural Science and Behaviour – E. Kandel and others. 23. Elements of Molecular Neurobiology – C U M Smith. 24. Fundamentals of Ecology and Environment, Second edition by Pranav Kumar and Usha

Mina, Pathfinder Publication New Delhi, India. 25. Elements of Ecology by Smith TM and Smith RL (2009), 7th ed. San Francisco CA:

Pearson Benjamin Cummings. 26. Concepts of Ecology by Kormondy E.J. (1996), 4th ed. W.H. Prentice-Hall. 27. Ecology: Global insights and investigations by Stiling P (2015), 2nd ed. McGraw-Hill

Education. 28. Environmental Science Laboratory Manual For Senior Secondary Course, National

Institute Of Open Schooling A-24/25, Institutional Area, Sector-62, Noida-201309 (U.P.)

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M. Sc. Part 1: Semester II (Theory) Paper 1: PSZO201: Chordates

Unit 1: Protochordates and Agnatha (15L) Objectives:

To equip learners with the knowledge of evolutionary progression of Protochordates.

To familiarize learner with the origin, morphology and phylogeny of the Protochordates.

Desired outcome:

Learners would be equipped with the understanding of morphology and phylogeny of Protochordates.

1.1 Protochordates

1.1.1 Origin and ancestry of Protochordates, similarities and differences with Invertebrates and Chordates

1.1.2 Comparison of characteristics between subphyla Urochordates and Cephalochordates

1.2 Urochordates

1.2.1 Life history of Herdmania and its phylogenetic affinities 1.3 Cephalochordates

1.3.1 General features and phylogenetic affinities 1.3.2 Life history of Branchiostoma

1.4 Ostracoderm

1.4.1 Salient features and biological significance 1.4.2 Interrelationship and affinities with fish

1.5 Cyclostomes

1.5.1 Resemblance with Cephalochordates, Vertebrates and differences from Fishes 1.5.2 Life history of Petromyzon

Unit 2: Phylogeny of Chordates (15L) Objectives:

To provide learners with the knowledge of evolutionary progression of Protochordates.

To familiarize learner with the origin, morphology and phylogeny of the Protochordates.

Desired outcome:

Learners would be equipped with the understanding of morphology and phylogeny of Protochordates.

2.1 Ancestry of Chordates 2.2 Pisces

2.2.1 General characters of Dipnoi and affinities with Fishes, Elasmobranchs and Amphibia

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2.2.2 Origin of air bladder and its relationship with tetrapod lungs 2.2.3 Deep sea adaptations of fishes 2.2.4 Origin of fins

2.3 Amphibia

2.3.1 Origin of Tetrapods 2.3.2 Pedomorphosis 2.3.3 Adaptive radiation in amphibians Lepospondyli and Lissamphibia

2.4 Reptilia

2.4.1 Origin of Reptiles and affinities with Amphibia 2.4.2 Terrestrial adaptations in Reptiles

2.5 Aves

2.5.1 Origin of Birds and affinities with Reptiles 2.5.2 Adaptive radiation in Birds – Ratitae (Flightless Birds) and Carinatae (Flying Birds) 2.5.3 Origin of Flight – Theory of Cursorial and Arboreal origin

2.6 Mammalia

2.6.1 Origin of Mammals 2.6.2 Phylogeny of terrestrial and aquatic mammals

Unit 3: Functional and Comparative Anatomy of Chordates (15L) Objectives:

To equip learners with the knowledge of evolutionary progression of chordates.

To familiarize learner with the origin, morphology and phylogeny of the chordates. Desired outcome:

Learners would understand evolution, morphology and phylogeny of chordates. 3.1 Comparative Anatomy of:

3.1.1 Chondrocranium 3.1.2 Splanchnocranium 3.1.3 Skull in different Vertebrates

3.2 Evolution and Comparative Anatomy of Excretory System of Chordates

3.2.1 Evolutionary development of Kidney Archinephros Pronephros Mesonephros Metanephros

3.3 Comparative Anatomy of Nervous System in Vertebrates

3.3.1 Brain 3.3.2 Sense organs – Eyes and Ears

3.4 Comparative Anatomy of Respiration

3.4.1 Gills, Skin and Lungs 3.5 Circulation: Heart and Aortic Arches

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3.6 Male and female reproductive and urinary ducts of Vertebrates (Cyclostomes, Teleost, Elasmobranchs, Amphibia, Reptilia, Aves and Mammalia) Unit 4: Assorted Topics on Chordates (15L) Objectives:

To introduce learners with some interesting topics of the chordates. Desired outcome:

Learners would obtain additional knowledge of diverse characteristics of various chordates

4.1 Retrogressive metamorphosis in Ascidians 4.2 Pisces

Evolutionary significance Crossopterygians, Placoderms Migration Lateral line sense organ and electric organs

4.3 Amphibia – Evolutionary significance of Labyrynthodonts 4.4 Reptilia

Evolutionary significance of Dinosaurs Venom apparatus and biting mechanism in snakes

4.5 Aves

Migration Flight adaptations

4.6 Mammalia

Dentition in mammals Habitat diversification Walking gait: Plantigrade, Digitigrade and Unguligrade Comparative account of Jaw suspension

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Paper 2: PSZO202 Developmental Biology – II Unit 1: Generalized Account of Reproductive Systems in Chordates (15L) Objective:

● To introduce to the learner the generalized account of reproductive systems and accessory structures in chordates.

Desired outcome:

● The learner will gain knowledge of reproductive systems in chordates. 1.1 Reproductive system

Urochordata – Tunicate Cephalochordata – Amphioxus Cyclostomata – Petromyzon Pisces – Catfish Amphibia – Frog Reptilia – Lizard Aves – Pigeon Mammalia – Human

1.2 Accessory Reproductive Glands – Human Unit 2: Physiology of Reproduction (15L) Objective:

● To acquaint the learner with physiology of reproduction in chordates. Desired outcome:

● The learners will get an idea of reproductive physiology in chordates. 2.1 Biochemistry of semen – Fish, Frog, Snake, Human.

2.1.1 Sperm capacitation, decapicitation – molecular mechanism and significance 2.2 Ovarian leuteinization and mammary gland differentiation – human 2.3 Pheromones – Pisces, Amphibia, Reptilia, Aves and Mammalia 2.4 Courtship behaviour in – Pisces, Amphibia, Reptilia, Aves, Mammalia Unit 3: Developmental Biology – Germ Cells, Stem Cells and Induced Breeding (15L) Objectives:

● To introduce to the learner the germ cell migration in chordates. ● To acquaint the learner with details of stem cells.

Desired outcome:

● The learner shall comprehend details of germ cell migration and understand the importance of stem cells.

3.1 Introduction to germ cells

Germ cell migration in Zebrafish, Frogs, Lizard, Chick and Mouse

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3.2 Stem cells – 3.2.1 Embryonic stem cells, Induced Pluripotent stem cells, Adult stem cells 3.2.2 Regeneration Therapy – Cardiac, bone and neuronal regeneration 3.2.3 Cryopreservation of stem cells – Concept, tools, techniques and application

3.3 Induced breeding in fish – technique and applications Unit 4: Animal Development – Impact of Environment and Evolution (15L) Objective: ● To introduce to the learner the impact of environment on development and its

evolutionary aspects. Desired outcome: ● The learner will be acquainted with environmental impact on development and

evolution of animals. 4.1 Environmental Cues and Impact on Development

Predator Induced Polyphenisms – Amphibian Temperature and Sex – Fish, Turtle and Alligator

4.2 Teratogenesis

Alcohol, Retinoic acid, Endocrine disruptors – Diethylstibestrol, Plastics – Nonylphenol, Bisphenol A and Heavy metals as teratogens

4.3 Developmental constraints on evolution – physical, morphogenetic and phyletic

4.3.1 Modularity of development – Example – Duffy blood group substance and Stickleback fish

4.4 Aging and Senescence – Causes, Consequences, and Therapeutics

4.4.1 Environmental and epigenetic causes of aging – Plastics, Pesticides, Heavy metals 4.5 Promoting Longevity: Role of telomerase: an overview

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Paper 3: PSZO203: Biochemistry and Biotechnology Unit 1: Fundamentals of Biochemistry (15L) Objective:

To provide learners an in-depth knowledge of the fundamentals of chemical foundations of biomolecules and their interactions.

Desired Outcomes:

Learners will have in-depth understanding of the fundamentals of chemical basis of life.

Learners will have knowledge of the structures, interactions, and importance of complex biomolecules and their significance in living system.

1.1 Water as the Basic Molecule of Life

1.1.1 Structure of atoms, molecules and chemical bonds 1.1.2 Molecular structure and solvent properties of water, tetrahedral geometry, hydrogen

bond, thermal properties of water and their biological significance 1.1.3 Ionization of water, Ion product of water (Kw), Concept of pH, Dissociation of weak

acids and weak bases, Henderson-Hasselbalch Equation, Titration curves of strong and weak acids, concept of buffer, buffers in biological systems

1.2 Biological Macromolecules

1.2.1 Central role of Carbon 1.2.2 Common ring structure, Chirality, and Isomerization in biomolecules 1.2.3 Composition, structure of monomers and polymers, functions of carbohydrates,

lipids, proteins, nucleic acids and vitamins 1.3 Interactions of Macromolecules

1.3.1 Stability of Proteins and Nucleic Acids; Stabilizing Interactions such as Vander Waals, Electrostatic, Hydrogen Bonding, Hydrophobic Interactions

1.3.2 Conformation of proteins: Ramachandran plot, secondary structure, domains, motif and folds, hydropathy index of amino acids, isoelectric point (pi) of proteins

1.4 Complex biomolecules

1.4.1 Glycoproteins: blood group determinants 1.4.2 Complex Lipids: Phospholipids, Sphingolipids, Gangliosides, Sterols and Waxes 1.4.3 Lipoproteins: classification and f unctions of chylomicrons, VLDL, LDL, HDL, and

free fatty acid-albumin complex Unit 2: Metabolism and Metabolic Pathways (15L) Objective:

To provide the learners knowledge of the metabolism of various biomolecules and the importance and interrelationships of metabolic pathways in the biological systems.

Desired Outcomes:

Learners’ will have in-depth understanding of the bioenergetics and metabolic pathways of various biomolecules.

Learners will gain knowledge of the regulatory mechanisms underlying various metabolic pathways.

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2.1 Biochemical Thermodynamics

2.1.1 Laws of thermodynamics, free energy, entropy, enthalpy, exergonic and endergonic reactions

2.1.2 Biological oxidation: Electron transport chain in mitochondria; oxidative phosphorylation – mechanism, uncoupling of oxidative phosphorylation and its significance

2.1.3 Free radicals, Antioxidants and Antioxidant system 2.2 Carbohydrate and Lipid Metabolism

2.2.1 Glycolysis and its energetics 2.2.2 Gluconeogenesis: Reaction sequence from pyruvate, gluconeogenesis from amino

acids, glycerol, propionate and lactate 2.2.3 Glycogen metabolism: Glycogenesis, Glycogenolysis 2.2.4 Significance of the pathways: Hexose Monophosphate (HMP) Shunt as a

multifunctional pathway; uronic acid pathway; glyoxalate cycle 2.2.5 Fatty acid metabolism: Oxidation of saturated even and odd carbon atom, and

unsaturated fatty acids, metabolism of cholesterol 2.3 Regulation of Metabolism

2.3.1 Regulation of metabolic flux by genetic mechanisms: Control of enzyme synthesis, constitutive and inducible enzymes; induction and repression of enzymes (lac operon and trp operon)

2.3.2 Regulation of metabolism by extracellular signals: nutrient supply, nutrient transport, endocrine control, neural control

2.4 Amino acid and Nucleic acid Metabolism

2.4.1 Nitrogen metabolism: Protein turnover, amino acid pool, nitrogen balance 2.4.2 Metabolism of Amino acids: Transamination, Deamination, Urea cycle 2.4.3 Formation of amino acids from Pyruvate, TCA cycle intermediates, Acetyl CoA 2.4.4 Nucleic Acid metabolism: De novo synthesis: synthesis of purine and pyrimidine

nucleotides; Salvage Pathway; Catabolism of purine and pyrimidine nucleotides Unit 3: Processes and Techniques in Biotechnology (15L) Objectives:

To provide the learners t horough knowledge on the genome organization, gene expression and its control in prokaryotes and eukaryotes.

To acquaint learners with the basic tools and techniques used in Biotechnology. Desired Outcomes:

Learners will be able to understand the processes for harnessing the potentials of living systems for betterment of mankind.

Learners will have an understanding of the processes and techniques used in development of biotechnology products.

3.1 Genome Organization

3.1.1 Organization of genome in prokaryotes and eukaryotes, C-value paradox and genome size

3.1.2 Complexity of viral, bacterial and eukaryotic genomes, Cot curves, repetitive and non-repetitive DNA sequences

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3.2 DNA replication, Gene expression in prokaryotes and eukaryotes

3.2.1 Molecular model of DNA replication 3.2.2 Transcription and translation in prokaryotes 3.2.3 Transcription and translation in eukaryotes

3.3 Control of gene expression in Prokaryotes & Eukaryotes

3.3.1 Small regulatory RNAs, small nuclear ribonucleoproteins (snRNPs), Transcription level control, RNA processing, Translational level control, post-translational control

3.3.2 Gene silencing, miRNA, RNA silencing pathways and DNA methylation 3.4 Methods in Biotechnology

3.4.1 Cloning using plasmid pUC18, pUC19, detection of recombinants by blue-white screening, cloning in bacteriophage, cosmid, BAC and YAC vectors

3.4.2 Chromosome walking, RAPD, AFLP, Microarrays Unit 4: Applications of Biotechnology (15L) Objective:

To provide learners the knowledge on the applications of biotechnology in various fields.

Desired outcome:

Learners will gain knowledge on the application of biotechnology in industries, medicines, agriculture and environment.

4.1 Industrial Biotechnology

4.1.1 Microbial fermentation, Microbial growth kinetics, Design of a fermenter, Organisms used in large scale fermentation.

4.1.2 Production of antibiotics – Cephalosporin, erythromycin; amino acids – proline, glutamate; vitamins – cyanocobalamine, riboflavin; Aspartame and Taxol

4.2 Medical Biotechnology: Molecular approaches in diagnosis and treatment

4.2.1 Peptide vaccines: synthetic drugs (engineered proteins) 4.2.2 Genetic immunization: Antisense DNA, Therapeutic ribozymes 4.2.3 Anti-idiotypic vaccine for cancer treatment 4.2.4 Monoclonal antibodies (mAbs) and their therapeutic applications 4.2.5 HIV therapeutic agents 4.2.6 Production of biopharmaceuticals from transgenic animals – Human Tissue

Plasminogen Activator (hTPA) and α-1Antitrypsin (AAT) 4.2.7 Pharmacogenomics and Nutrigenomics: An overview and applications

4.3 Agricultural Biotechnology

4.3.1 Plant Tissue culture methods–Broad outline and applications of Pollen culture & Protoplast culture

4.3.2 Bacillus thuringiensis based bio-pesticides, genetic engineering of herbicide resistance trait in crops, genetic engineering of β-carotene biosynthetic pathway in golden rice

4.3.3 Genetic engineering of biological nitrogen fixation (Nitrogen as ecluster and hydrogenase genes), Genetic engineering drought/salinity tolerance trait in crops

40

4.4 Environmental Biotechnology 4.4.1 Effluent treatment, Bioremediation, phytoremediation, Biosensors, Biofuels 4.4.2 Cartagena protocol on biosafety – General features with respect to objectives,

precautionary principle and live modified organisms (LMO)

41

Paper 4: PSZO204: Research Methodology

Unit 1: Principles of Scientific Research (15L) Objectives:

To acquaint learners with the principles of scientific research and methods of conducting scientific enquiry.

To inculcate scientific temperament and research aptitude among the learners. Desired outcome:

Learner will be able to formulate research problem and concept research design.

Learner will be able to execute the research problem. 1.1 Basic concepts of research

1.1.1 Definition 1.1.2 Characteristics of research (controlled, rigorous, systematic, valid and verifiable,

empirical and critical) 1.1.3 Objectives of research 1.1.4 Types of research:

a) Pure and applied b) Structured and unstructured c) Descriptive, correlational, explanatory, exploratory, historical, comparative and

experimental 1.2 Formulating research problem

1.2.1 Reviewing literature: Using library, computerized searches, evaluating literature 1.2.2 Developing a research problem: Importance, sources, considerations, steps,

defining a problem, formulate objectives 1.2.3 Identifying variables: Introduction, types of variables, measurement scales 1.2.4 Hypothesis: Definition, function, characteristics, types (H0, HA), Principles of

hypothesis, framing hypothesis, errors in hypothesis, research question versus research hypothesis, hypothesis versus non-hypothesis research

1.3 Research design

1.3.1 Definition and purpose 1.3.2 Characteristics of good research design 1.3.3 Sampling strategy and sampling size determination, eliminating extraneous

variables 1.3.4 Types of study design: study designs in quantitative research (cross-sectional,

before and after, longitudinal, retrospective, prospective, retrospective-prospective, experimental, non-experimental, quasi-experimental)

Unit 2: Data Management and Analysis (15L) Objectives:

To introduce learners to various statistical tools for analysis of research data.

To familiarize learners with the in-silico approach for data management. Desired outcome:

The learner will be able to develop skill set for organisation and effective presentation of data.

42

The learner will be able to choose correct statistical test.

The learner will be able to perform statistical analysis indifferent fields of research using various computer programs.

2.1 Methods of data collection

2.1.1 Primary sources 2.1.2 Secondary sources

2.2 Data Classification and Presentation

2.2.1 Classification of data (Geographical, Chronological, Qualitative, Quantitative) 2.2.2 Tabulation of data (one-way table, two-way table, complex table) 2.2.3 Type of charts (Line, Column, Scatter plot, Box plot, Heat Map)

2.3 Concepts of Measurements

2.3.1 Univariate (standard deviation, variance, quartiles) 2.3.2 Bivariate (correlation and regression) 2.3.3 Multivariate (ANOVA: one-way, two-way) 2.3.4 Level of significance and p-value 2.3.5 Normal distribution, Skewness, Kurtosis, Outliers

2.4 Testing of Hypothesis

2.4.1 Null hypothesis, Alternative hypothesis 2.4.2 Type 1 and Type 2 Errors 2.4.3 Testing of Hypothesis (single population mean, two population means) 2.4.4 One-tailed and Two-tailed tests

2.5 Parametric and Non-parametric tests

2.5.1 Parametric tests: t test, z test, F test 2.5.2 Non-parametric tests: Chi-square test, Mann-Whitney test, Kruskal-Wallis test,

Friedman Test, Wilcoxon signed-rank test Unit 3: Scientific Communication (15L) Objectives:

To enable learners to develop skills for effective research communication.

To enable learners to publish research paper in journals with good impact factors. Desired outcome:

The learner will be able to get an insight of scientific communication documentation and will become familiar with good scientific journals.

3.1 Research planning

3.1.1 Project Proposal and research funding agencies – Research grants, scholarships and funding (CSIR, DBT, DST, DST- INSPIRE Fellowship, ICMR, INSA, BRNS, MoEFCC, UGC-RFSMS, Fulbright Fellowships for Indian students, Lady Tata Memorial Trust, EPA, Bill and Melinda Gates Foundation, Wellcome Trust, Erasmus Mundus)

3.1.2 Use of reference management software (MS Word / Zotero / Mendeley) 3.1.3 Scientific writing –Thesis and Dissertation

3.2 Documentation for approval from Institutional Animal Ethics Committee (IAEC) and

43

Institutional Biosafety Committee (IBSC) 3.2.1 Documents for registration, renewal and reconstitution of IAEC as per CPCSEA

guidelines 3.2.2 Proposal layout for permission from IAEC for use of animals in research 3.2.3 Proposal layout based on recombinant DNA safety guidelines from IBSC 3.2.4 Proposal layout for use of transgenic animals in research

3.3 Publications and Research Journals

3.3.1 Types of research journals 3.3.2 Impact factors of Journals 3.3.3 Predatory Journals 3.3.4 Review process - benefits and drawbacks of single blind, double blind and open

peer review process 3.3.5 Understanding “h – index” and “i10 – index” 3.3.6 SCOPUS, Google Scholar, Web of Science 3.3.7 Process to obtain ISSN and ISBN

Unit 4: Model organisms and their applications (15L) Objectives:

To introduce to learners the key species which are used as model organisms in research.

To acquaint the learners with special techniques developed for studying these model organisms.

To make them aware of the contributions of model organisms to basic biology, genetics, development and diseases.

Desired outcome:

The learners will be able to understand the special features of different animals that have made them useful as a model system.

The learners will become familiar with the specific applications of model organisms for various biological processes.

The learners will be able to appreciate how experimentation with these organisms has enhanced the understanding of the basis of human diseases.

4.1 Introduction: Concept key features and importance of model organisms 4.2 Earthworm

4.2.1 Biology and genome 4.2.2 Model for studies on regeneration

4.3 Caenorhabditis elegans

4.3.1 Body plan of the worm, genetics, genome, cultivation, advantages and limitations 4.3.2 Techniques used for genetic modification, Mutant characterization, discovery of

RNAi 4.4 Daphnia sp.

4.4.1 Genome, epigenetic phenotypic variation 4.4.2 Advantage and limitations 4.4.3 Model for studies in toxicology

44

4.5 Drosophila melanogaster 4.5.1 Genome, advantages and limitations 4.5.2 Model for cytogenetics, development, neuroscience, human diseases and

therapeutic drug discovery 4.6 Danio rerio (Zebra fish)

4.6.1 Genome, advantages and limitations 4.6.2 Insights on embryology, ageing and toxicology

4.7 Mus musculus (Mouse)

4.7.1 G enomics, advantages and limitations 4.7.2 Model for studies on physiology, development, ageing, human diseases (Cancer

and Diabetes) and behaviour research 4.7.3 Transgenic and germ line transgenic mouse models, genetically engineered mouse

models (GEMMs): Knock-In and Knock-out mouse models

45

M. Sc. Part 1: Semester II Practical 1 PSZOP201: Chordates

1. Museum specimens / Photographs / Pictures / Slides:

i. Protochordates: Study of Doliolum, Herdmania, Ascidia, Botryllus ii. Adaptive radiation in Reptiles-Turtles, Crocodile, Tuatara, Snakes and Lizards iii. Adaptive radiation in Mammals – Talpa (mole), Tarsius, Armadillo, Camel and Sperm

whale 2. Comparative anatomy of digestive system in vertebrates. 3. Comparative study of Pelvic and Pectoral girdle in vertebrates.

4. Types of jaw suspension in vertebrates- Autodiastylic, Holostylic, Amphistylic and Autostylic.

5. Types of vertebrae – Acoelous, Procoelus, Opisthocoelus, Amphicoelus, Amphiplatins, Heterocoelus, Axis and atlas vertebrae.

6. Phylogenetic analysis of phenotypic characters from given data in chordates. 7. Construction of phylogenetic tree with given ancestors.

8. Pigeon – Study / Virtual dissection of i. Flight muscles ii. Digestive system iii. Respiratory system iv. Circulatory system v. Excretory system vi. Reproductive system – male and female

9. Field visit to National park / Sanctuary / Museum / Zoo or any other suitable ecosystem to study vertebrates.

46

Practical 2 PSZOP202: Developmental Biology II

1. Identification of fish developmental stages–egg, larva, juvenile (fry, fingerling and adult). 2. Measurement of fish ova diameter using oculometer. 3. Preparation of histological slides – fish ovary / testis. 4. Study of metamorphosis in Amphibia. 5. Histology of male and female accessory reproductive glands – Human – Prostate gland,

Bulbourethral gland and placenta [Permanent slides]. 6. Analysis of testicular extract/ semen – Fish / Chicken / Goat.

i. pH, Viscocity, Agglutination. ii. Sperm count and motility – Fish / Chicken / Goat. iii. Hypo-osmotic swelling of sperm – Normal / Abnormal.

7. Study of stem cells from chick embryo – staining and identification of cells.

47

Practical 3 PSZOP203: Biochemistry and Biotechnology 1. Titration curve of weak acid. 2. Titration curve of strong acid. 3. Quantitative estimation of proteins from the given sample using Bradford method. 4. Determination of glucose by Benedict’s quantitative reagent (Titrimetric method). 5. Isolation and determination of glycogen in the given tissue (liver / skeletal muscle / kidney)

by Anthrone method. 6. Determination of saponification value of fats/oils. 7. Estimation of the number of bacteria in the given culture by nephelometry. 8. Preparation of beads by immobilization of yeast cells in calcium alginate. 9. Monitoring of Invertase activity in bioreactor column of immobilized yeast cells by DNSA

method. 10. Restriction digestion of the given DNA sample and separation of the fragments by

agarose gel electrophoresis. 11. Southern blotting technique (assembly of the sandwich/ mechanical blotting). 12. Determination of effect of minimal and enriched media on growth curves of E. coli.

48

Practical 4 PSZOP204: Research methodology

1. Write a proposal to obtain permission for use of model animal in research from IAEC.

(Print copy to be submitted during practical exam and assessed by viva.) 2. Write a grant proposal to a funding agency. (Print copy to be submitted during

practical exam and assessed by viva.) 3. Evaluation of journal / research paper metrics:

a. Impact factor (from the given data) b. h – index (from the given data) c. Percent plagiarism (using online tools)

4. Interpret the given data (qualitative / quantitative) and represent as graph / chart. 5. Problems based on parametric tests and non-parametric tests. (to be performed using

MS Excel / SPSS / Minitab) 6. Calculation of correlation co-efficient and plotting regression line from given biometry

data using MS Excel / SPSS / Minitab. 7. Calculation of diversity indices (Shannon and Simpson) using MS Excel / PAST3. 8. Descriptive / Inferential statistics using online statistical tests – GraphPad Prism 9. https://www.graphpad.com/data-analysis-resource-center/

10. To study the characteristics and applications of animal models used in pre-clinical research.

11. Small animal models: Rat, Transgenic mice, Nude mice, Hamster. 12. Large animal models: Rabbit, Cynomolgus macaque, Rhesus macaque, Vervet / African

green monkey, Marmoset, Chimpanzee. 13. Visit to an animal house in a research institution and submission of report.

49

References Semester 2 PSZO201 1. Introduction to Genera Zoology, Volume II– K. K. Chaki, G. Kundu, S. Sarkar (NCBA) 2. Vertebrate Zoology– F. B. Mondal (Oxford IBH) 3. Modern TextBook of Vertebrates – R. L. Kotpal (Rastogi) 4. Alexander, R. M. The Chordata. Cambridge University Press, London. 5. Barrington,E. J. W. The biology of Hemichordata and protochordata. Oliver and Boyd,

Edinborough. 6. Kingsley, J. S. Outlines of comparative autonomy of vertebrates. Central Book Depot,

Allahabad. 7. Kent, C. G. Comparative anatomy of vertebrates. 8. Smith, H. S. Evolution of chordate structure. Hold Rinehartand Winstoin Inc., New York. 9. Torrey, T. W. Morphogenesis of vertebrates. John Wiley and Sons Inc., New York. 10. Romer, A. S. Vertebrate Body, III Ed. W. B. Saunders Co., Philadelphia 11. Young, J. Z., Life of mammals, The Oxford University Press, London 12. Colbert, E.H., Evolution of the vertebrates. John Wiley and Sons Inc., New York. 13. Weichert, C. K. and Presch, W. Elements of Chordate anatomy.4th Edn. McGraw Hill

Book Co., New York. 14. Montagna, W. Comparative anatomy. John Wiley and Sons Inc. 15. Andrews, S. M. Problems in vertebrate evolution. Academic Press, New York. 16. Waterman, A. J. Chordata structure and function. Macmillan Co., New York. 17. Lovtrup, S. The phylogeny of vertebrate. John Wiley & Sons, London 18. Barbiur, T. Reptiles and Amphibians: Their habits and adaptations. Hongton Miffin

Co., New York. 19. Smyth. Amphibia and their ways. The Macmillan Co., New York. 20. Jordan and Verma. Chordate Zoology, S. Chand Publication.

50

PSZO202 1. Comparative embryology of the vertebrates 1 – 2 (1953) McGraw – Hill Book company,

New York. 2. Comparative reproductive biology - HeideSchatter and Gheorghe Constantinescu –

Blackwell Publishing. 3. Human reproductive system – Encyclopedia Britannica by Richard J. Harrison. 4. Animal Physiology – Withers. 5. Animal Physiology - Hoar 6. Developmental Biology- Scott F. 7. Gilbert, 9th Edition. 8. Animal Physiology - Giese 9. Chordates- Jordan and Verma 10. Chordates- Parker and Haswell 11. Chick embryology- Patten 12. Chordate Zoology- Kotpal 13. Chordate Zoology-P. S. Dhami and J. K. Dham 14. Sex pheromones in amphibians-a review Vet.Med-Czech, 50,2005(9); 385-389 15. http://www.eurekaselect.com/node/156190/related-ebooks 16. https://embryo.asu.edu/pages/sperm-capacitation 17. https://www.sciencedirect.com/book/9780124366435/handbook-of-stem-cells 18. https://ivf.net/ivf/a-textbook-of-in-vitro-fertilization-and-assisted-reproduction-the-bourn-

hall-guide-to-clinical-and-o418.html 19. https://www.britannica.com/science/teratogenesis 20. https://link.springer.com/chapter/10.1007/978-3-642-45532-2_15 21. https://www.elsevier.com/books/handbook-of-the-biology-of-aging/schneider/978-0-12-

627871-2 22. https://www.whatisepigenetics.com/fundamentals/

51

PSZO203 Biochemistry

1. Puri, Dinesh (2014). Textbook of Medical Biochemistry (3rd

Edition). 2. Bhagavan N. V. and Chung-Eun Ha (2015).Essentials of Medical Biochemistry (2nd

Edition). 3. Harper’s Illustrated Biochemistry. 4. Satyanarayana U. and Chakrapani (2014).Biochemistry. 5. Outlines of Biochemistry, (5th Edition) – 2006 – Eric E. Conn, Paul K. Stumpf, George

Bruening. 6. Lehninger, L. Albert, David, L. Nelson, Michael, M. Cox (1993). Principles of Biochemistry,

CBS Publishers and Distributors, Delhi. 7. Stryer, L (1988), Biochemistry, W.H. Freeman and Company, New York. 8. Cooper, T. G (1977), The Tools of Biochemistry, Wiley Interscience Publication, John

Wiley and Sons, New York. 9. Smith (1983), Principles of Biochemistry, (7th Edition), Mc Graw Hill (Mammalian

Biochemistry), New York. 10. Voet, D. & Voet, J (1995), Biochemistry, John Wiley and Sons, New York. 11. Basic Concepts in Biochemistry – Hiram F Gilbert –McGraw Hill Publications.

12. Fundamentals of Biochemistry – J L Jain, Sunjay Jain, Nitin Jain – S. Chand. Biotechnology 1. Molecular Cell Biology (5th Edition) – Lodish, Berk, Matsudaira, Kaiser, Krieger, Scott,

Zipursky, Darnell – WH Freeman. 2. Cell & Molecular Biology: Concepts & Experiments (6th Edition) – Gerald Karp. 3. iGenetics – A molecular approach (3rdEdition) – Peter J Russell – Pearson Education Inc. 4. Molecular Biotechnology – Principles and applications of recombinant DNA (3rd Edition)

– Bernard R Glick &Jack J Pasternak – ASM Press. 5. Principles of Fermentation Technology (2nd Edition) – Peter F Stanbury, Allan Whitaker,

Stephen J Hall – Elsevier Publications. 6. Basic Biotechnology (3rd Edition) – Colin Ratledge & Bjorn Kristiansen – Cambridge

University Press. 7. Medical Biotechnology – S. N. Jogdand – Himalaya Publishing House. 8. Microbial Biotechnology (2nd Edition) – Alexander Glazer & Hiroshi Nikaido – Cambridge

University Press. 9. Plant Tissue Culture: Theory and Practice – S. S. Bhojwani & M. K. Razdan – Elsevier. 10. Molecular Biology and Biotechnology– KG Ramawat & Shaily Goyal – S. Chand. 11. Aspartame Information Center.www.aspartame.org 12. Genetic engineering of taxol biosynthetic genes in Saccharomyces cerevisiae. a.

Biotechnology and Bioengineering 93(2): 212-24 • February 2006 13. The Golden Rice Project http://www.goldenrice.org/ Additional Reading:

1. Molecular Biology of the Cell – Alberts et al., - Garland Science.

2. Molecular Biology of the Gene – Watson et al., Benjamin Publications. 3. Genes VIII – Benjamin Lewin –Oxford Press. 4. Molecular Biology – Freifelder – Narosa Publication House. 5. Textbook of Biotechnology – H K Das – Wiley India Publication.

52

PSZO204 Principles of Scientific Research: 1. Graduate research - A guide for students in Life Sciences. 2nd edition. Robert V. Smith 2. Research methods for Biosciences. 3rd edition. Debbie Holmes, Peter Moody, Diana Dine,

Laurence Trueman 3. Experimental designs for Life Sciences. 4th edition. Graeme D., Ruxton and Nick Colegrave 4. Research methodology - Step by Step Guide for Beginners. 3rd edition. Ranjit Kumar 5. Research methodology - Tools and techniques, Bridge Centre 2015 Data Management and Analysis: 1. The practice of statistics in life sciences - Brigitte Baldi and David Moore, W. H. Freeman. 2. Mahajan’s methods in Biostatistics for Medical students and Research Workers - Bratati

Banerjee, Jaypee Publishers. 3. Biostatistics: Basic concepts and methodology - Wayne Daniel and Chad Cross, Wiley. 4. Biostatistical analysis - Jerrold H. Zar, Pearson. 5. Statistics for people who (think they) hate statistics – Neil J. Salkind, SAGE Publications. 6. Discovering Statistics using IBM SPSS Statistics – Andy Field, SAGE Publications. 7. IBM SPSS Statistics Step by Step – Darren George and Paul Mallery, Taylor & Francis. 8. Statistics for Ecologists using R and Excel – Mark Gardener, Pelagic Publishing. Scientific Writing: 1. Thesis and Dissertation Writing in a Second Language a handbook for supervisors - Brian

Paltridge and Sue Starfield, Routledge Publishers. 2. The process of research writing - Steven Krause – Eastern Michigan University. 3. Animal - Use Research IAEC Project Proposal & animal imports Guidelines – NCBS Animal

Care and Resource Center. 4. Academic Writing and Publishing – A Practical Handbook – James Hartley. 5. How to Improve Your Journal and Have Greater Impact – James Testa – Thomson Reuters. 6. https://beckerguides.wustl.edu/authors/hindex 7. https://www.elsevier.com/en-in/reviewers/what-is-peer-review 8. https://www.thehindu.com/sci-tech/science/what-is-a-predatory journal/article21039351.ece Model Organisms and their applications: 1. Introduction to Genetic Analysis: Griffiths, Wessler, Lewontin, Carroll. WH Freeman

Company, New York. 2. Biology. E. P. Solomon, L. R. Berg, D. W. Martin, Thompson Brooks/Cole. 3. Bier, E. 2005. Drosophila, the Golden Bug, Emerges as a Tool for Human Genetics. Nature

Reviews Genetics 6: 9-23. 4. Howe K et al. The zebra fish reference genome sequence and its relationship to the human

genome. Nature 2013. 5. Pennisi, E. (1998). Worming secrets from the C. elegans genome. Science, 282, 1972-1974. 6. Fly: http://flybase.bio.indiana.edu:82/ 7. Worm: http://www.expasy.ch/cgi-bin/lists?celegans.txt 8. Zebrafish: http://www.ncbi.nlm.nih.gov/genome/guide/D_rerio.html 9. Mouse: http://www.informatics.jax.org/

53

Modality of Assessment:

A) Internal Assessment‐ 40%

Sr. No.

Evaluation type Total Marks

1. One Assignments/Case study/Project 25

3. Active participation in routine class instructional deliveries 05

4. Attendance – (0-25%=0 marks, 25-50%=1mark,50-75%=3 marks and 75-100%= 5 marks)

05

5. Overall conduct as a responsible student, manners, skill in articulation, leadership qualities demonstrated through

organizing co‐curricular, etc.

05

B) External examination 60%

a) Semester End Theory Assessment‐ 60% 60 Marks i) Duration – These examinations shall be of two and half hours duration for each paper. ii) Theory Question Paper Pattern:

There shall be five questions each of 12 marks. On each unit there will be one question and the first one will be based on entire syllabus.

All questions shall be compulsory with internal choice within the questions. Each question will be of 18 to 20 marks with options.

Question may be subdivided into sub‐questions a, b, c… and the allocation of marks depend on the weightage of the topic.

b) Practicals 50 Marks

54

University of Mumbai M.Sc. ZOOLOGY

Semester I / Semester II EXAMINATION

Course PSZO Maximum Marks: 60 Duration: 2.5 Hours Marks Option: 90 Question 1: Based on Unit I to IV (Mixed Questions) Question 2: Based on Unit I Question 3: Based on Unit II Question 4: Based on Unit III Question 5: Based on Unit IV Instructions:

i. All questions are compulsory. ii. All questions carry equal marks. iii. Draw neat and labeled diagrams wherever necessary.

1. Answer any three questions from the following( Based on all 4 units)

a. 04

b. 04

c .

04 d 04 04

e 04

2. Answer any two questions from the following(Based on Unit1) a. 06

b. 06

c. 06

3. Answer any two questions from the following (BasedonUnit2) a. 06

b. 06

c.

06

D

4. Answer any two questions from the following (Based on Unit 3) a. 06

b. 06 c. 06

5. Answer any two questions from the following (Based on Unit 4) a. 06

b. 06

c. 06

55

PRACTICAL EXAMINATION PSZOP101 Time: 10 am to 2 pm Total Marks: 50 Q.1 To study Nervous / Reproductive System of Clamworm / Prawn / Pila / Starfish / Balanoglossus. 06 Q.2 To Study Digestive / Excretory system of Clamworm / Prawn / Pila / Starfish / Balanoglossus. 06 Q.3 Identify and describe the larva of: (preferably slides / specimens available) 04 a. Annelida / Arthropoda b. Mollusca / Echinodermata Q.4 Identify and describe fossils: a. Annelida / Arthropoda 03 b. Mollusca / Echinodermata / Hemichordata 03 c. Annelida / Arthropoda / Mollusca / Echinodermata / Hemichordata 03 (This specimen c should be out of syllabus, but should show clear characters of the phylum) Q.5 Making fossil cast out of clay / muddy soil and viva based on it. 05 Q.6 Field visit report and viva based on field visit. 10 Q.7 Viva based on theory. 05 Q.8 Journal. 05

56

PRACTICAL EXAMINATION PSZOP102 Time: 10 am to 2 pm Total Marks: 50 Q.1 Major question: 14 To study conjugation and binary fission in Paramecium from given culture.

OR To study life cycle of Drosophila. Q.2 Minor question: 08 To observe development of C. elegans.

OR To observe stages of Tribolium or Sitophilus. Q.3 Identification: (3 marks each) 18

a) Gemmule / Germ layer b) Larva – Porifera / Cnidarian / Annelida c) Larva – Mollusca / Crustacea d) Larva – Echinodermata / Hemichordata e) Life Cycle of Butterfly / Moth f) Coelom

Q.4 Viva. 05 Q.5 Journal. 05

57

PRACTICAL EXAMINATION PSZOP103 Time: 10 am to 2 pm Total Marks: 50 Q.1 DNA isolation / Kappa particles in Paramecium / Maternal effects in Drosophila. 10 Q.2 Identify and describe: 10

Parental care / Evolution of Brood Parasitism – Cuckoo or Crow / Reciprocal altruism – Vampire Bat / Thumping of Ground Gene – Rabbit / Klepto parasitic fledglings associated with reduced success in host family in barn owl.

Q.3 Identify the following: (2 marks each): 14 a) Comparison of Hemoglobin b) Type of Fossil c) Type of Fossil d) Example of Cloning e) Example of Cloning f) Syndrome g) Syndrome

Q.4 Two problems based on gene mapping and mitotic index. 06 Q.5 Viva. 05

Q.6 Journal. 05

58

PRACTICAL EXAMINATION PSZOP104 Time: 10 am to 2 pm Total Marks: 50 Q.1 To determine concentration of Iron as a pollutant from sample / sample solution by redox titration or spectrophotometric method. 12

OR To estimate the amount of dust (particulate matter) deposited on the leaves of roadside plants.

OR To detect heavy metals from the sample water – Zinc / Lead / Copper. Q.2 Identify and describe 12

a) Chronobiology b) Imaging technique c) Imaging technique d) Climate change / global warming indicator animal e) Climate change / global warning indicator animal f) Anatomical regions of brain involved in stress and emotion

Q.3 Assignment –submission of report on pulse rate after stress. 06 Q.4 Submission of report on visit to nearby DNA barcoding laboratory / medical Imaging center. 05 Q.5 Environment audit report (Green audit / EIA of small selected area). 05 Q.6 Viva-voce. 05 Q.7 Journal. 05

59

PRACTICAL EXAMINATION PSZOP201

Time: 10 am to 2 pm Total Marks: 50 Q.1 a. Study of Pigeon – digestive / circulatory / respiratory system. 06

b. Study of Pigeon – excretory / male reproductive / female reproductive / flight muscles. 04

Q.2 Identify and describe as per the instructions. 15

a. Identify and describe (any Protochordate) b. Identify and comment on its adaptive radiations(Reptilia / Mammalia) c. Identify and comment on comparative account of digestive system (Fish / Frog / Lizard /

Pigeon / Rat). d. Identify and comment on any type of jaw suspension. e. Identify and comment on type of (vertebrae / pelvic girdle / pectoral girdle).

Q.3 Make a phylogenetic analysis of phenotypic characters from given data / Construct phylogenetic tree on the basis of given data. 05 Q.4 Field visit report and viva based on it. 10 Q.5 Viva-Voce based on theory. 05 Q.6 Journal. 05

60

PRACTICAL EXAMINATION PSZOP202

Time 10: 00 AM to 2: 00 PM Total Marks: 50 Q.1 Estimate the pH and viscosity/ pH and agglutination/Viscosity and agglutination from Fish / Chick / Goat semen. 10

OR Determine the sperm count and motility from Fish / Chick / Goat semen.

OR Determine the hypo-osmotic swelling of sperm – Normal / Abnormal. Q.2 Determine the diameter of the ova from suitable fish using occulometer. 15

OR Prepare block / ribbon / stained slides for histological study of fish ovary / testis.

OR Stain and identify the stem cells from chick embryo. Q.3 Identify and describe 15

a) Histology of accessory reproductive glands / organ (Prostrate / Bulbourethral / T.S of placenta)

b) Histology of accessory reproductive glands /organ (Prostrate / Bulbourethral / T.S of placenta)

c) Study of metamorphosis in Amphibia ( any one stage) d) Fish developmental stages – Egg / larva e) Fish developmental stages – fry / fingerling

Q. 4 Viva-Voce. 05 Q. 5 Certified journal. 05

61

PRACTICAL EXAMINATION PSZOP203

Time: 10.0 AM to 2.00 PM Total Marks: 50 Q.1 Determine the pKa of weak acid/ Perform titration of strong acid and prepare the titration curve. 16

OR Estimate the invertase activity in bioreactor column of immobilized yeast cells by DNSA method.

OR Estimate glucose by Benedict’s quantitative reagent (Titrimetric method). Q.2 Estimate quantitatively the proteins from the given sample using Bradford method. 14

OR Isolate and determine glycogen in the given tissue (liver/ skeletal muscle / kidney) by Anthrone method.

OR Determine saponification value of fats /oils.

OR Determine effect of minimal /enriched media on growth curves of E. coli.

OR Estimate the number of bacteria in the given culture by Nephelometry. Q.3 Prepare calcium alginate beads for immobilization of yeast cells. 10

OR Perform restriction digestion of the given DNA sample and separate the fragments by Agarose Gel Electrophoresis.

OR Perform the assembly for Southern blotting technique (Mechanical / Physical blotting). Q.4 Viva-voce. 05 Q.5 Certified Journal. 05

62

PRACTICAL EXAMINATION PSZOP204

Time: 10:00 am – 02:00 pm Total Marks: 50

Q.1 Perform a parametric test on the given data using MS Excel / SPSS / Minitab and interpret the result. 12

OR Perform a non-parametric test on the given data using MS Excel /SPSS / Minitab and interpret the result.

OR Calculate correlation co-efficient and plot a chart with regression line from the biometry data provided using MS Excel / SPSS / Minitab and interpret the result.

OR Calculate biodiversity indices of the given data using MS Excel / PAST3 and interpret the result. Q.2 A. Perform descriptive statistics of the given data using MS Excel / SPSS / Minitab and interpret the result. 06

OR Interpret the type of data provided and represent it in relevant chart using MS Excel / SPSS / Minitab.

OR Perform descriptive / inferential statistics using online resources provided at Graph Pad Prism – Quick Calcs. B. Calculate from the given data– a) Impact factor and b) h-index. 04

OR Estimate percent plagiarism from the text provided and comment on the results. Q.3 Identify the model organisms and comment on their applications (a– d). 12 a and b – small animal models c and d – large animal models Q.4 Report of visit to animal house in a research institution. 05

Q.5 Submission of proposal to IAEC / Application for grant from funding agency and viva based on it. 06 Q.6 Certified Journal 05

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M.Sc. in Zoology: SEMESTER III and IV

Credit Based Semester and Grading System

to be implemented from the Academic Year 2013-2014.

 

PREAMBLE 

 

BOS in Zoology during its meeting constituted a pyramid committee, to revise the syllabi in Zoology, with  Dr.  M.  K.  Pejaver  as  the  Chairperson  and  Senior  Teachers  from  affiliated  Colleges  as  Jt. Chairperson,  one  each  for  UG  and  PG  programmes.  The  class‐wise  syllabus  committees  were constituted  in accordance with  inclusive policy of  the BOS with an aim  to provide  faculty at  large hands on training and exposure to work on syllabus committees which will go a  long way  in taking our subject ahead in future when these experienced staff members would shape the subject after a decade.  With  the  introduction  of  Credit  Based  Semester  and  Grading  System  and  continuous evaluation  consisting  of  components  of  internal  assessment  and  external  assessment  by  the esteemed University, the syllabus  in Zoology was revised for M.Sc. Sem  I and  II to be  implemented with effect from 2012‐13, after approval by concerned authorities of the University. 

 

Vide University Circular No. APD/Misc.‐01/407/of 2011 dated 12/12/2011, contents of letter from K. P.  Singh,  Joint  Secretary, UGC No. D.O.F1‐1/2009‐(CPP‐II)  dated  29/11/2011 were  notified  to  the faculty in Zoology. As per the letter an expert committee was constituted by the UGC to look into the issue of discontinuation of dissection of  live animals  in the  laboratory experiments  in Zoology/ Life Sciences at UG and PG  levels. The guidelines prepared by  the expert committee and approved by UGC were notified with a viewpoint to ensure compliance of the guidelines.  

  

A special meeting of Heads of Zoology Departments of all  the Colleges affiliated  to  the University was  convened  on  17th  August  2012  for  deliberation  on  recommendation  of  expert  committee appointed  by  the  UGC  regarding  the  discontinuation  of  dissection  of  live  animals  in  laboratory experiments in Zoology / Life Sciences at UG and PG level. 

 

In accordance with the deliberations in the above meeting, draft syllabus for M.Sc. SEMESTER‐III and IV in Zoology, suitably revised, to be implemented in the Credit Based Semester and Grading System was prepared by the committee under the guidance of pyramid committee. The draft was circulated among the heads and senior teachers of the Department of Zoology of various colleges for approval and suggestions. 

 

In meeting  of  the  BOS  geld  on  12th  December,  the  draft  was  approved  and  it  was  resolved  to implement the revised syllabus of Zoology at M.Sc. SEMESTER‐III and IV and make  it effective from the Academic Year 2013‐2014 after approval from concerned authorities of the University. 

 

 

 

Chairman 

Board of Studies in Zoology 

                                                                                                                              University of Mumbai

M.Sc. Semester III and IV Zoology- Biotechnology--Oceanography and Fishery Science

Credit Based Semester and Grading System. To Be Implemented from the Academic Year 2013-2014.

Semester –III

Theory

Course Unit TOPIC Credits L / Week

PSZOBT301

I

The implications of recombinant DNA technology of commercial products and microbial synthesis

4

1

II

Large scale culture & production from recombinant microorganisms & genetically engineered animal cells

1

III Medical Biotechnology 1 IV Environmental Biotechnology I 1

PSZOBT302

I Genome management and analysis

4

1

II Manipulation of gene expression in prokaryotes

1

III Bioinformatics 1 IV Animal biotechnology and Human therapies 1

PSZOOCN303

I General Oceanography

4

1 II Physical Oceanography 1 III Chemical Oceanography 1 IV Biological Oceanography 1

PSZOOCN304

I Planktology

4

1 II Fish and Fishery Science 1 III Biotechnology in Fishery and Biometric

Studies 1

IV Aquaculture 1 16 16

Practicals PSZOBT3P1 Practicals based on PSZOBT 301 2 4 PSZOBT3P2 Practicals based on PSZOBT 302 2 4 PSZOOCN3P3 Practicals based on PSZOOCN 303 2 4 PSZOOCN3P4 Practicals based on PSZOOCN 304 2 4 Total 08 16 TOTAL 24 32    

 

 

 

 

 

.

SEMESTER –IV

Theory Course Unit TOPIC Credits L / Week

PSZOBT401

I Microbial synthesis of commercial products

4

1 II Large scale culture & production for

industrial biotechnology 1

III Agricultural Biotechnology 1 IV Environmental Biotechnology II 1

PSZOBT402

I Genome management

4

1 II Manipulation of gene expression in

eukaryotes 1

III The human genome project 1 IV Regulations and patents in biotechnology 1

PSZOOCN403

I General Oceanography

4

1 II Physical Oceanography 1 III Chemical Oceanography 1 IV Biological Oceanography 1

PSZOOCN404

I Planktology

4

1 II Fish and Fishery Science 1 III Biotechnology in Fishery and Biometric

Studies 1

IV Aquaculture 1 16 16

Practicals PSZOBT4P1 Practicals based on PSZOBT4P1 2 4 PSZOBT4P2 Practicals based on PSZOBT4P2 2 4 PSZOOCN4P3 Practicals based on PSZOOCN 403 2 4 PSZOOCN4P4 Practicals based on PSZOOCN 404 2 4 Total 08 16 TOTAL 24 32

M.Sc. Semester III and IV Zoology- Biotechnology--Oceanography and Fishery Science

Credit Based Semester and Grading System. To Be Implemented from the Academic Year 2013-2014.

SEMETER – III

THEORY

PSZOBT301

PSZOBT301: Basics of Industrial & Environmental Biotechnology I

Unit I: The implications of recombinant DNA technology of commercial products and microbial synthesis 15

1.1. The implications of recombinant DNA technology 1.1.1 *General account on applications of biotechnology 1.1.2 *Commercialization of biotechnology & biotech companies 1.1.3 Prospects of novel food technology 1.1.4 Economics of microbial biotechnology 1.1.5 Areas of significant public concern: Antibiotic resistance marker gene, transfer

of allergies, pollen transfer from GM plants, social, moral & ethical issues associated with GMOs.

1.2 Amino acids & their commercial use – production strain, process of L-glutamate, L-aspartate, L-phenylalanine, L-tryptophan.

Unit II: Large scale culture & production from recombinant microorganisms 15

& genetically engineered animal cells 2.1. Large scale culture & production from recombinant microorganisms: 2.1.1 Batch fermentation 2.1.2 Fed batch fermentation 2.1.3Continuous fermentation 2.1.4 *Maximizing the efficiency of fermentation process 2.1.5 Harvesting, disrupting & downstream processing 2.2. Large scale culture & production from genetically engineered animal cell cultures:

2.2.1Design of bioreactors for large scale animal cell culture-Batch, Fed batch

2.2.2 Mammalian cell lines & their characteristics 2.2.3 Media for the cultivation of mammalian cells 2.2.4 *Commercial products produced with mammalian cell culture

Unit III: Medical Biotechnology 15 3.1. Sub-unit vaccines

3.1.1 *Sub-unit Vaccine production against viruses-Herpes simplex, Bovine foot & mouth disease virus

3.1.2 Peptide vaccines-synthetic drugs (engineered proteins) 3.1.3 Genetic immunization-DNA vaccines, Antisense DNA, Therapeutic ribozymes

3.1.4 *Live recombinant vaccines 3.1.5 *Attenuated vaccines against Cholera, Salmonella sp.

3.1.6 Vector vaccines-Vaccine directed against viruses- Rabies virus G-protein, Hepatitis B surface antigen

3.1.7 Anti-idiotypic vaccine for cancer treatment 3.2. Monoclonal antibodies (mAbs) & therapeutic applications: 3.2.1 mAbs for prevention of rejection of transplanted organs 3.2.2 Treatment of bacterial blood infection 3.2.3 Human monoclonal antibodies 3.2.4 Hybrid human-mouse monoclonal antibodies 3.2.5 HIV therapeutic agents 3.2.6 Anti-tumour antibodies Unit IV: Environmental Biotechnology I 15 4.1. Biomass utilization 4.1.1 Microorganisms in lignocellulose degradation 4.1.2 Isolation of prokaryotic & eukaryotic cellulase gene 4.1.3 Manipulation of cellulase gene 4.1.4 Production of single cell proteins by using biomass as raw material 4.1.5 Commercial production of fructose and alcohol from biomass 4.1.6 Improvements of fructose and alcohol production 4.1.7 Fuel ethanol from biomass 4.2. Bioremediation of aerobic compounds 4.2.1 Characteristics of xenobiotics in the environment

4.2.2 Characteristics of aerobic microorganisms for degradation of organic pollutants 4.2.3 Genetic engineering of biodegradative pathways- Manipulation by transfer of plasmid, manipulation by gene alteration

4.2.4*Degradation of xenobiotic compounds-petroleum products, n-alkanes, alkenes, cycloaliphatic compounds, aromatic hydrocarbons, polyaromatic hydrocarbons, chlorinated organic compounds (aliphatic & aromatic)

*marked topics are to be taken for seminar

PSZOBT302

PSZOBT302: GENETIC ENGINEERING TECHNIQUES AND ITS APPLICATONS

Unit I: Genome management and analysis 15

1.1 The Basic tools of genetic engineering 1.1.1 Chemical Synthesis of DNA-Oligonucleotide synthesis by

Phosphoramidite method, Synthesis of genes 1.1.2 *DNA Sequencing -- Maxam-Gilbert method, Sanger’s

dideoxynucleotide method, By using bacteriophage M13 By Primer walking

1.1.3 Polymerase chain reaction and its advantages 1.2 Cloning Vectors

1.2.1 *General purpose plasmid vectors (pUC19, pBR322)(Bacterial Vectors)

1.2.2 Bacteriophage and cosmid vectors 1.2.3 Yeast artificial chromosomes (YACs)

1.3 Analysis of genome/proteome 1.3.1 DNA fingerprinting/physical mapping/pulsed field gel electrophoresis 1.3.2 Analysis of the proteome 1.3.3 Analysis of mRNA transcripts

Unit II: Manipulation of gene expression in prokaryotes 15

2.1 Promoters of gene expression in prokaryotes

2.1.1 Prokaryotic gene expression

2.1.2 Isolation of functional promoters

2.1.3 Promoter selection with E.coli plasmid pBR316

2.1.4 *Promoter selection with plasmid pKO1

2.1.5 Gene expression from strong and regulatable promoters

2.2 Expression of cloned genes in prokaryotes

2.2.1 Increasing protein production and secretion

2.2.2 *Inclusion bodies and fusion proteins

2.2.3 Unidirectional tandem gene arrays

2.2.4 Translation expression vectors

2.2.5 Increasing protein stability

Unit III: Bioinformatics 15

3.1 Uses and application of computers in biological sciences

3.2 *DNA profiling: cDNA and EST’s (expressed sequence tags)

3.3 Basic research with DNA microarrays and its application in

healthcare.

3.4 Biomedical genome research and pharmaco genomics

3.5 *Random amplified polymorphic DNA (RAPD)

3.6 Human genomic variation-SNP’s (single nucleotide

polymorphisms,SNP’s and disease; QTL (quantitative trait loci)

and its relation to SNP’s

3.7 Satellite DNA and its types

Unit IV: Animal biotechnology and Human therapies 15 4.1 Animal Biotechnology

4.1.1 *Transgenic animals and their applications:

Mice as model system for human diseases and as test case model,Cows, pigs, sheep, goats as biopharmaceuticals

Transgenic insects and birds

4.1.2 Recombinant DNA technology to prevent animal diseases

4.1.3 Conservation biology-Embryo transfer

4.1.4 Regulation of transgenic animals and patenting genetically

engineered animals

4.2 Human therapies

4.2.1 Tissue engineering: Skin, liver, pancreas

4.2.2 *Xenotransplantation

4.2.3 Antibody engineering

4.2.4 Cell adhesion based therapies: Integrins, Inflammation, Cancer

and metastasis

4.2.5 Targeted gene replacement for correcting a mutated gene

4.2.6 Site directed mutagenesis

*marked topics are to be taken for seminar

PSZOOCN303- GENERAL, PHYSICAL, CHEMICAL AND BIOLOGICAL OCEANOGRAPHY UNIT I: GENERAL OCEANOGRAPHY 15 L 1.1 Terminology of submarine topography Continental shelf, continental slope, submarine canyons, submarine mountain ranges, Guyots and trenches with special reference to the Indian Ocean and adjacent seas. * 1.2 A general knowledge of typical oceanographic research vessel and its equipments, oceanographic labs and stations of the world and India. UNIT II: PHYSICAL OCEANOGRAPHY 15 L 2.1 Physical properties of sea water: Salinity, Chlorinity, Temperature, Light, Density, Pressure, Salinity-Temperature- Density relationship (STD) . 2.2 Oceanographic circulation: Ekman spiral, geotropic current, westward intensification with dynamic topography. UNIT III: CHEMICAL OCEANOGRAPHY 15 L * 3.1 Composition of sea water- constancy of its composition and factors affecting the composition, major and minor constituents, trace elements and their biological role. 3.2 Dissolved gases in the sea water and their role in the environment, CO2 system, dissolved O2 and oxygen profile, hydrogen sulphide. 3.3 Nutrients in the ocean, their cycles and factors influencing their distribution a) Nitrogen b) Phosphorus c) Silicon. UNIT IV: BIOLOGICAL OCEANOGRAPHY 15 L *4.1 Sea as a biological environment. *4.2 Division of marine environment. 4.3 a) Marine biotic diversity: Plankton, Nekton, Benthos- brief account Implications of species richness, measuring diversity, quadrients of species diversity, models explaining diversity gradient. *b) Intertidal organisms and their zonation. 4.4 Effect of physical factors on marine life a) Light: photosynthesis, colouration, structural adaptations, bioluminescence.

b) Temperature: tolerance, geographical distribution, size, calcium precipitation, metabolism, bipolarity, tropical submergence and periodicity.

c) Salinity: tolerance and distribution, size, buoyancy and osmoregulation. d) Currents: role in nutrition, transportation and propogation. *e) Marine bacteria and their role.

*marked topics are to be taken for seminar

SEMESTER III

PSZOOCN304- PLANKTOLOGY, FISH, FISHERY SCIENCE AND AQUACULTURE

UNIT I: PLANKTOLOGY 15L

1.1. Classification of Plankton. Adaptation to planktonic life.

Factors influencing the distribution and abundance, plankton bloom, patchiness, vertical distribution and red tide.

1.2. *Diurnal migration of zooplankton. Inter-relationship between phyto and zooplankton.

UNIT II: FISH AND FISHERIES SCIENCE 15L

2.1. An overview of fish classification as per Francis Day and FAO.

2.2. a) Major commercial fisheries: Elasmobranchs (shark and ray)

Teleosts: Sciaenoids, Indian salmon, Seer fish, Mackerel, Sardine, Carangids, Tuna, Sole fish, Harpodon, Ribbon fish fisheries. b) *Crustacean fisheries: Prawns (penaeid and non penaeid), Shrimps, Lobster and Crab. c) *Molluscan fisheries.

UNIT III: BIOTECHNOLOGY IN FISHERY AND BIOMETRIC STUDIES 15L

3.1. Fish stock improvement through selective hybridization.

3.2. Gene transfer technology in fish: General steps for developing transgenic fishes. Gene transfer by microinjection, electroporation, transfer of transgenes by injection with pantropic retroviral viruses, fish antifreeze protein gene, promoter in the production of growth hormone. *Characterization of transgenic fish. (Identification of transgenic fish and expression of transgenes). Gene transfer in common carp and channel fish.

UNIT IV: AQUACULTURE 15L 4.1. *History, scope and importance of aquaculture.

Aquaculture practices in India. Cultivable organisms for aquaculture and criterion for their selection.

4.2. Different systems of aquaculture such as Pond Culture, Cage Culture, Pen

Culture, Running Water Aquaculture, Raft Culture,Aquaranching. 4.3. Impact of aquaculture on environment. *marked topics are to be taken for seminar

SEMESTER III- PRACTICALS

Course Code PSZOBT3P1 & PSZOBT3P2 (Based on PSZOBT301 and PSZOBT302)

1) Demonstration of aseptic technique: Work place for aseptic handling, packing

glassware (flasks, test tubes, pipettes, petridish) for sterilization, aseptic transfer of liquids (pipetting from flask to test tube)

2) Preparation of LB agar plate, slant, butt & demonstration of streaking technique using bacterial culture to obtain isolated colonies.

3) Determination of viable cell count in the given culture of bacteria by dilution & spreading technique.

4) Using mini-prep method isolate plasmid DNA from the given strain of bacteria & show the purity of the isolate by performing agarose gel electrophoresis.

5) To estimate the number of bacteria in the given culture by nephelometry.

PSZOOCN3P3

1) Physical and chemical oceanography: (Uniform methods for all colleges to be followed) Determination of physico-chemical parameters:

1) Salinity (Argentometric and conductivity method) 2) Dissolved oxygen, 3) Carbon dioxide. 4) Nitrates-nitrites. 5) Silicates. 6) Phosphate-phosphorus.

2) Textural features: Sediment analysis- size fraction (sand, silt, clay) 3) Identification of foraminiferans and radiolarians from sand. 4) Estimation of primary productivity by light and dark bottle. 5) Identification of intertidal organisms: a) Rocky shore- Patella, Chiton, Fissurella, Mytilus species, Perna viridis, Cardium,

Balanus, Gorgonids, Littorina and Corals. b) Sandy shore: Solen, Umbonium, Oliva, Pea crab, Fiddler crab, Molluscan shells, Star fish and Balanoglossus. c) Muddy shore: Lingula, Chaetopterus, Arenicola, Tubiculus worm and Mud skipper.

PSZOOCN3P4

1) Laboratory procedure for quantitative estimation of plankton settling method, wet weight method, weight displacement method, counting method. 2) Identification of Zooplankton permanent slides (Noctiluca, Obelia medusa, Zoea, Zoea porcelina, Copepods, Mysids, Echinoderm larvae, Nauplius, Sagitta, Doliolum, Salpa, Fish eggs and larvae, Jelly fish, Physalia, Porpita) 3) Study of fecundity-maturation studies. 4) Plotting the frequency polygon by ova diameter measurement. 5) Identification and classification of Marine fishes List of Marine fishes Elasmobranchs 1. Family- Carcharidae Carcharias sps. Zygaena malleus 2. Family- Rhinobatidae Rhynchobatus djeddensis 3. Family- Trygonidae Trygon uarnak Teleost 4. Family- Percidae Lutianus johnii, Therapon sps., Pristipoma maculatum, Synagris japonicus, Gerres filamentosus 5. Family- Squamipinnes Scatophagus argus 6. Family – Mullidae Upenoides vittatus 7. Family- Polynemidae Polynemus tetradactylus 8. Family- Sciaenidae Pseudosciaena diacanthus, Sciaena sps. 9. Family- Trichuridae Trichurus savala/ haumela 10. Family- Carangidae Caranx rottleri, Chorinemus toloo 11. Family- Stromatidae Pampus chinensis, Pampus argenteus 12. Family- Scombridae Rastrelliger kanagurta, Cybium guttatum 13. Family- Trachinidae Sillago sihama 14. Family- Cottidae Platycephalus punctatus 15. Family- Gobidae Periophthalmus sps., Boleophthalmus sps.

16. Family- Sphyraenidae Sphyraena acutippinis 17. Family- Mugillidae Mugil sps. 18. Family- Gadidae Bregmaceros sps. 19. Family- Pleuronectidae Psettodes erumei, Cynoglossus elongatus 20. Family- Siluridae Arius dussumieri 21. Family- Scopelidae Saurida tumbil, Harpodon nehereus 22. Family- Sombresocidae Belone stongylurus, Hemiramphus sps. 23. Family- Clupeidae Pellona feligera, Clupea longiceps 24. Family- Chirocentridae Chirocentrus dorab 25. Family- Muraenesox Muraenesox sps. Note: Minimum number of animals to be used for experiment

. SEMESTER -IV

Zoology-Biotechnology--Oceanography and Fishery Science Credit Based Semester and Grading System.

To Be Implemented from the Academic Year 2013-2014.

PSZOBT401: Basics of Industrial & Environmental Biotechnology II

Unit I: Microbial synthesis of commercial products 15 1.1. Microbial synthesis of commercial products

1.1.1 Organic acids & their commercial applications – Citric acid, gluconic acid, lactic acid.

1.1.2 Antibiotics – Cloning antibiotic biosynthetic gene by complementation & other methods.Synthesis of novel antibiotics & improving antibiotic production. *Aminoglycosides & their uses

1.1.3 Polysaccharides: Bacterial polysaccharides: General properties & their commercial applications-

Dextran, Xanthan, Alginate.

Genetic engineering for the large scale production of Xanthan gum & its modification.

*Marine polysaccharides: General properties & their commercial application- Agar &agarose, Chitosan

1.1.4 Polyesters: Polyhydroxyalkanoates (PHA)-Biosynthesis of PHA, Biopol-commercial biodegradable plastic

Unit II: Large scale culture & production for industrial biotechnology 15

2.1. Biotransformations 2.1.1 Selection of biocatalyst-screening & use of novel existing biocatalyst 2.1.2 Genetic modification of existing biocatalyst (Indigo biosynthesis) 2.1.3 Biocatalyst immobilization- Methods of immobilization- Cross linking, supported immobilization,

adsorption & ionic binding, covalent coupling, lattice entrapment 2.1.4 Immobilized soluble enzymes & suspended cells 2.1.5 Immobilization of multi-enzyme systems & cells 2.1.6 *Immobilized enzyme reactors- Batch reactors, continuous reactors 2.1.7 Analytical enzymes-

Enzymes in diagnostic assays: Test strip systems & Biosensors-Electrochemical & optical type

Unit III: Agricultural Biotechnology 15 3.1. Agricultural Biotechnology: 3.1.1 *Nitrogen fixation

3.1.2Nitogenase-Component of nitrogenase; Genetic engineering of nitrogenase cluster

3.1.3 Hydrogenase-Hydrogen metabolism 3.1.4 Genetic engineering of hydrogenase gene

3.1.5 Nodulation-Competition among nodulation organisms, genetic engineering of nodulation gene

3.1.6 Microbial insecticides-Toxins of Bacillus thuringiensis, mode of action & use of thuringiensis toxins, thuringiensis toxin gene isolation, genetic engineering of Bacillus thuringiensisstrains& cloning of thuringiotoxin gene.

3.1.7*Developing insect resistant, virus resistant & herbicide resistant plant 3.1.8 Algal products: Fuels from algae, marine natural products & their medical

potential-anticancer, antiviral compounds, antibacterial agents. Unit IV: Environmental Biotechnology II 15 4.1. Bioabsorption of metals (Recovery from effluents) 4.1.1 *Bioabsorption by fungi, algae, moss & bacteria

4.1.2 Mechanism of bacterial metal resistance & genetic engineering for specific proteins

4.1.3 Bioreactors for bioabsorption-packed bed, fluidized bed, rotating disc, single blanket, sequential reactors

4.1.4 Phytoremediation &its use in biotechnology 4.2. Bioleaching of metals 4.2.1 Biochemical mechanism of bioleaching 4.2.2 Extraction from mixtures 4.2.3 Types of bioleaching 4.2.4 Methods for bioleaching-Tank & heap bioleaching 4.2.5*Microorganisms used for bioleaching

*marked topics are to be taken for seminar

PSZOBT402: Genome management, manipulation, regulations and patents in biotechnology

Unit I: Genome management 15

1.1 The Basic tools of genetic engineering 1.1.1 Gene transfer techniques: Protoplast fusion, calcium phosphate,

precipitation, electroporation, liposome, ligand mediated, gene gun or biolistic approach,viral mediated

1.1.2 Selection and screening of recombinants

1.1.3 *Nucleic acid probes and hybridization, Southern blotting and

Northernblotting

1.1.4 Immunological assays for identification of gene product, Westernblot

1.2 Cloning Vectors

1.2.1 *Retrovirus and SV40 vectors

1.2.2 Special purpose vectors- Expression vectors, Secretion vectors,

Shuttle or bi-functional vectors, single stranded phage and phagemids

Unit II: Manipulation of gene expression in eukaryotes 15

2.1 Eukaryotic gene expression

2.2 *Introduction of DNA into fungi-yeast and filamentous fungi

(fungal transformation)

2.3 Heterologous proteins production in yeasts

2.4 Heterologous proteins production in filamentous fungi

2.5 Cultured insect cells expression systems-

Baculovirus transfer vector

2.6 *Mammalian cell expression systems-

Human Papova BK virus shuttle vector

Unit III: The human genome project 15

3.1 *The human genome, scope and goals of the project

3.2 Genetic linkage maps, chromosome walking, restriction mapping

3.3 Polymorphic DNA markers

3.4 Restriction fragment length polymorphism (RFLP) and its uses

3.5 Physical maps, Sequence tagged sites

3.6 Integrating genetic linkage and physical maps

3.7 *Mapping human diseases

3.8 Positional cloning: Getting closer to a disease causing gene

3.9 Testing for exons

3.10 Limitations of positional cloning

Unit IV: Regulations and patents in biotechnology 15

4.1 Regulating recombinant DNA technology

4.2 *Regulatory requirements – safety of genetically engineered

foodsChymosin, tryptophan, bovine somatotropin

4.3 Regulation environmental release of genetically engineered

organism(GEO). Ice minus Pseudomonas syringae

4.4 Regulatory agencies and laws for product regulation

4.5 Risk assessment: How much risk?

4.6 *Open field tests of GEO

4.7 Development of policy for Human gene therapy

4.8 Patenting biotechnology inventions

a) What constitutes the patent? b) The patent process c) The conditions to be satisfied for an invention to be patentable

:Novelty, Inventiveness, Usefulness d) Patenting in different countries, types of inventions that are not

patentable in India e) What is Paris convention? Principal features of Paris convention f) Patenting multicellular organisms g) Patenting and fundamental research

*marked topics are to be taken for seminar

PSZOOCN403: GENERAL, PHYSICAL, CHEMICAL AND BIOLOGICAL OCEANOGRAPHY UNIT I: GENERAL OCEANOGRAPHY 15 L 1.1 Oceanographic instruments:

Grab (Peterson and Van veen) for benthos collection, naturalist’s dredge (Ekman Sanders deep sea anchor dredge), trawl, plankton nets and continuous plankton sampling system, Reversing Nansen bottles, Reversing thermometer, Salinometer, Secchi disc, Stempel’s pipette and dilution jar, underwater photography, remote sensing and satellite imaging, SCUBA apparatus.

*1.2 Oceanographic Expeditions: Challenger, Indian Ocean and Antarctic. 1.3 Law of sea. UNIT II: PHYSICAL OCEANOGRAPHY 15 L 2.1 Vertical circulation: wind induced circulation, Thermohaline circulation and

upwelling of water. 2.2 Waves: Characteristics of waves, deep water and shallow water waves, transitional

waves, wind generated waves, internal waves and Tsunami *2.3 Tides: Tides generating forces, equilibrium theory of tides, dynamic theory of tides, tides as a source of power. * 2.4 Currents: Types of currents, major currents of the world, Coriolis effect and El Nino effect. UNIT III: CHEMICAL OCEANOGRAPHY 15 L 3.1 Impact of anthropogenic activities: A) a) Pollution- Domestic sewage, industrial/heavy metals. Agricultural- fertilizers and pesticides.

b) Oil pollution. c) Ocean dumping. d) Radioactive and Thermal waste. B) Reclamation.

UNIT IV: BIOLOGICAL OCEANOGRAPHY 15 L

4.1 Resources from the sea: A) Mineral resources: a) Continental margin. b) Deep sea mud oozes and manganese nodules. c) Oil, gas and sulphur deposits and role of ONGC. B) Bioactive compounds from the sea. C) Scientific and economical aspect of seabed exploration and mining. *marked topics are to be taken for seminar

SEMETER – IV PSZOOCN404: PLANKTOLOGY, FISH, FISHERY SCIENCE AND AQUACULTURE

UNIT I: PLANKTOLOGY 15L

1.1. Marine algae and plankton in relation to fisheries. Indicator species

1.2. Methods of collection, preservation and analysis of plankton. 1.3. *Marine Bio-deterioration: Fouling and Boring organisms.

UNIT II: FISH AND FISHERIES SCIENCE 15L

2.1. Population Dynamics Abundance in population and fishery. Fishery catches and fluctuation. M.S.Y., Optimum Yield, Age Composition, Population Growth, Population Models. 2.2. *Socio-economics of fishermen.

UNIT III: BIOTECHNOLOGY IN FISHERY AND BIOMETRIC STUDIES 15L

3.1. Statistical methods: Collection of data, Sampling methods, Presentation data, Measurement of central tendancy and dispersion, Frequency distribution, Analysis of variance and co-variance, Correlation regression, Theory of probability, Tests of significance, Chi-square test.

3.2. * Measurement of fish: a) Measurement of length and weight b) Morphometric measurements c) Merestic counts d) Biometric index UNIT IV: AQUACULTURE 15L

4.1. Hatchery and grow out practices for cultivable species of freshwater fishes (Indian major carps and exotic carps) and prawns (Macrobrachium rosenbergii), Culture of Air breathing fishes. 4.2. Integrated aquaculture and sewage fed fishery Hatchery and growout practices for the culture of brackish water fishes (Chanos chanos and Lates calcarifer), Prawns (Penaeus monodon and Penaeus indicus). 4.3. *Present status of sea farming in India

Culture of molluscs, clams, oyster (edible and pearl) and Mussels, Echinoderms (sea cucumber), sea weeds. *marked topics are to be taken for seminar

SEMESTER IV PRACTICALS

SEMESTER IV Practicals

Course Code PSZOBT4P1 & PSZOBT4P2 Based on PSZOBT401 and PSZOBT402

1) Immobilize Yeast cells in calcium alginate & prepare a bioreactor column to

demonstrate Invertase activity in the bioreactor column. 2) Restriction-digest the given DNA sample &demonstrate the separation of fragments

by performing agarose gel electrophoresis. Interpret the results by comparing with the standard digests provided.

3) Demonstrate the western blotting technique for the given sample of protein. 4) To plot a growth curve for the microorganisms provided. 5) Demonstrate the effect of medium on growth curves of given microorganism, using

two different media (minimal & enriched).

PSZOOCN4P3 Based on PSZOOCN403

1) Oceanographic instruments: a) Nansen reversing bottle. b) Deep sea reversing thermometer. c) Bathythermometer. d) Drift bottle. e) Ekman’s current meter. f) Secchi disc. g) Plankton nets: Standard net, Hensen net and Clarke Bumpus net. h) Stemple pipette and counting slide. i) Nekton sampling device-trawls. j) Benthic sampling devices-dredges, grabs and corers.

2) Detection of heavy metals: a) Zinc b) Lead

c) Copper. 3) Food and feeding in fish. 4) Identification of crafts and gears.

PSZOOCN4P4

Based on PSZOOCN4P4 1) Preparation of Zooplankton mountings. 2) Collection of marine algae and preparation of herbaria (at least five different forms). 3) Biometric studies of fish/ prawn A. Study of relationship between total length and standard length/head length/body depth length/body weight. B. Calculate correlation (standard length and total length, head length and total length, body depth and total length). Calculate the index values for various relationships. 4) Identification of fouling and boring organisms (Limnoria sps., Lepas, Balanus, Caprella, Teredo, Littorina, Crassostrea, Pellaria/ Sertularia). 5) Identification and classification of fresh water fishes (Rohu, Catla, Mrigal, Tilapia, Gourami) and fresh water giant prawn (Macrobrachium rosenbergii). 6) Crustacean fishery (Penaeus monodon, P. indicus, M. monoceros, P. stylifera, Solenocera indica, Nematopaleomon, Acetes indicus). 7) Molluscan fishery (Meretrix, Perna viridis, Katelysia sps., Crassostria sps., Xancus pyrum, Solen kempi, Cuttle fish and gastropods). 8) Visit to aquaculture centres, boat building yards, processing plants and marine biological institutions (Excursions or study tours) Students Activity a. Collection of molluscan shells b. Preparing herbaria from marine algae (atleast 5) c. Preparation of shrimp pickle

Note: Minimum number of animals to be used for experiment REFERENCES:

Semester III & IV Biotechnology

1. Johan E. Smith, Biotechnology, 3rd Edition, Cambridge Univ. Press 2. Colin Rateledge and Bjorn Kristiansen, Basic Biotechnology, 2nd Edition, Cambridge

Univ. Press 3. Susan R. Barnum, Biotechnology – An Introduction, Vikas Publishing House

4. Bernard R. Glick and Jack J. Pasternack, Molecular Biotechnology – Principles and applications of recombinant DNA, ASM Press, Washington DC.

5. Alexander N. Glazer and Hiroshi Nikaido, Microbial Biotechnology – Fundamentals of applied microbiology, W. H. Freeman and Co, New York

6. InduShekar Thakur, Environmental Biotechnology – Basic concepts and applications, I. K. International Pvt. Ltd, Mumbai, New Delhi

7. John A. Thomas (Ed.), Biotechnology and safety assessments, 2nd Edition, Taylor and Francis

8. S. S. Purohit, Biotechnology – Fundamentals and applications, 3rd Edition, Agrobios, India

9. Patent Facility Centre (PTC) Technology information, Forecasting and Assessment Council (TIFAC), Department of Science and Technology, New Delhi

10. R. S. Crespi; Patents – a basic guide to patenting biotechnology, Cambridge Univ. Press

11. R. E. Speir, J. B. Griffiths, W. Berthold (Ed), Animal Cell Technology – Products of today, prospects of tomorrow, Butterworth –Heinman Publishers

12. Martin Fransman, GerdJunne, AnnemiekeRoobeek (Ed), The Biotechnology revolution?, Blackwell Scientific Publishers

13. Terence Cartwright, Animal Cells as Bioreactors, Cambridge Univ. Press 14. A. Rosevear, John F. Kennedy, Joaquim M. S. Cabral, Immobilized enzymes and

cells, Adam Hilger Publishers, Bristol and Philadelphia 15. Micheal P. Tombs and Stepan E. Harding, An Introduction to polysaccharide

biotechnology 16. T. A. Brown, Gene Cloning – An Introduction, 3rd Edition, Nelson Thornes 17. Bob Old and S. B. Primrose, Principles of Gene Manipulation, 5th Edition, Wiley

Blackwell Publishers 18. U. Satyanarayan, Biotechnology, 2007 Reprint, Uppala Author Publisher Interlink

REFERENCES: Oceanography and Fishery Science

Semester III & IV 1. Svedrup et al., The Oceans. 2. Nair N.B. and Thampi D.H., Atextbook of marine ecology, T-M-H. 3. Harold Thurman, Introductory oceanography, Prentice Hall. London. 4. Qasim S.Z., Glimpses of Indian Ocean, Sangum Bodes Ltd. London. Navya Printers, Hyderabad. 5. Michael King, Fisheries Biology assessment and management, Fishing News Publishers, 1995. 6. R. Gordob Pirje, Oceanography. 7. Newell and Newell, Marine Plankton. 8. Jhingran, Fish and fisheries

9. P. Michal, Ecologival methods for field and laboratory investigations. 10. R.V. Tait, Marine zoology, Oxford press. 11. David Ross, Introduction to Oceanography. 12. Carl Schliper, Research method in marine biology. 13. B.F. Chapgar, Sea Shore life of India, SIDGWICK and JACKSON, London 14. D.V. Bal and K.V. Rao, Marine fisheries of India, T-M-H. 15. Russel and Young, The Seas 16. Kurian and Sebastian, Prawn and prawn fisheries of India. 17. M. Krishna Pillai. Introduction to Planktology, Himalaya Publishing 18. A.A. Fincham. Basic marine biology, British Museum Natural History. 19. Latha Shenoy. Course manual in fishing technology, CIFE, Versova, Mumbai. 20. Jefferey F. Raymond, Plankton and productivity, Vol. I and II. 21. J.S.Levington, Marine Biology, Function, biodiversity, ecology. Oxford University Press. 22. Wealth of India, Vol. IV, CSIR Publications. 23. S.P. Biswas, Manual of methods in fish biology, South Asian publishers private Ltd., New Delhi. 24. J.P. Rilcy and R, Chester, Introduction to marine chemistry, Academic Press, London and New Delhi. 25. American Public Health Association-2000. 26. J.V.R. Pillai, Aquaculture principles and plasia, Blackwell Scientific pub. 27. Das P. and Jhingran A.C.G., Fish genetics in India. 28. Colin E. Purdon, Genetics and Fish breeding, Chapman and Hall. 29. Schroder J.J., Genetics and Mutagenesis of fish, Chapman and Hall. 30. P. Bensam. Development of marine fishery sciences in India, Daya publishing House. N.B : I) It is pertinent to note that we have to adhere strictly to the directions as given in the UGC Circular F14-4/2006 (CPP-II). II)Apart from the institutional Animal Ethics Committee (IAEC) and any other Committee appointed by a Competent Authority/Body from time to time, every college should constitute the following Committees :

1) A Committee for the Purpose of Care and Supervision of Experimental Animals (CPCSEA) and

2) A Dissection Monitoring Committee (DMC) Composition of DMC shall be as follows :

i) Head of the Concerned Department (Convener/Chairperson) ii) Two Senior Faculty Members of the concerned Department iii) One Faculty of related department from the same College

One or two members of related department from neighboring colleges

Practicals paper pattern

Semester III

Zoology- Biotechnology-Physiology

Course Code PSZOBT3P1 

Q1) Determination of viable cell count in the given culture of bacteria by  dilution & spreading technique. (DAY 1)                                                                                                         (25) MARKS 

                                                                           OR 

Q1) Using mini‐prep method isolate plasmid DNA from the given strain of bacteria & show the purity of the isolate by performing agarose gel electrophoresis. (DAY 1)                (25) MARKS 

Q2) To estimate the Demonstration of aseptic technique: Work place for aseptic handling, packing glassware (flasks, test tubes, pipettes, petridish) for sterilization, aseptic transfer of liquids (pipetting from flask to test tube.   (DAY 2)                                          (15) MARKS 

Q3)   Viva                                                                            (05) MARKS 

Q4) Journal                                                                             (05) MARKS 

 

Practical  Course Code PSZOBT3P2 

Q1)  Preparation of LB agar plate, slant, butt &  demonstration of streaking technique using bacterial culture to obtain isolated colonies.   (DAY 1)                                              (25) MARKS 

Q2) Estimate number of bacteria in given culture of nephelometry. (DAY 2)               (15) MARKS 

 Q3)   Viva                                                                               (05) MARKS 

Q4) Journal                                                                               (05) MARKS 

PRACTICAL EXAMINATION PSZOOCN3P3 

1. (A)  Determination  of Physio‐chemical parameter salinity/D.O./CO2 /Nitrates‐

Nitrites/Silicates/Phosphate‐Phosphorus.               10 

                                                      OR                                         

(A) Estimation of primary productivity by light and dark bottle.                                                     10 

 

(B)   Foraminiferan and radiolarian shells (any four)                          05   

       2.    Minor – Sediment analysis                                                                                                      07 

       3.    Identify and describe (any 6 Intertidal Organism) (6 X  3 )                                               18 

       4.    Viva voce                                                                                                                                   05  

       5.    Journal                                                                                                                                       05 

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PRACTICAL EXAMINATION PSZOOCN3P4 

 

1. Major 

(A) Fish identification ( 1 from Elasmobranch, 4 from Teleost)                                       15  

(B) Fish identification as per Francis day volume                                                               05 

2. Minor  

(A) Study of maturity, Plankton settling method/ weight method/weight  

displacement method/ counting method and study of fecundity and 

 maturation studies                                         08 

Or 

Plotting the frequency polygon by ova diameter measurement                             08 

       3.    Identification  ( 4 identification 3 marks each )                                      12                                            

       4.    Viva voce                                                                                                                                   05  

       5.    Journal                                                                                                                                       05 

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Course Code PSZOBT4P1 

Q1) Demonstrate the effect of medium on growth curves of given microorganism, using enriched media. (DAY 1)                (25) MARKS 

          OR 

Q1) Demonstrate the effect of medium on growth curves of given    microorganism, using minimal media. (DAY 1)                                                     (25) MARKS 

Q2) Immobilize Yeast cells in calcium alginate, prepare beads & keep them overnight in activation medium (DAY 1)                    (15) MARKS 

Q3)   Viva                     (05) MARKS 

Q4) Journal                          (05) MARKS 

 

 

 

Practical  Course Code PSZOBT4P2 

Q1) Prepare a bioreactor column to demonstrate Invertase activity in the bioreactor column. (DAY 2)                   (25) MARKS 

           

Q2) Restriction‐digest the given DNA sample & demonstrate the separation of fragments by performing agarose gel electrophoresis. Interpret the results by comparing with the standard digests provided. (DAY 2)                                                                                       (15) MARKS 

        OR 

Q2) Demonstrate the western blotting technique for the given sample of protein.  

(DAY 2)                                                                                                                     (15) MARKS 

Q3)   Viva                                                              (05) MARKS 

Q4) Journal                                                               (05) MARKS 

Semester IV 

PRACTICAL EXAMINATION PSZOOCN4P3 

   Total Marks: 50   

 

1. Major Experiment 

Identification of Oceanographic instrument (3 identification 4 marks each)                12 

2. (A) Detection of heavy metals – zinc/ Lead/ Copper                                                          10 

(B) Food and feeding in fish                                                                                                    06 

       3.    Identification  ( 4 identification 3 marks each 2 from crafts & 2 from gears)              12                                             

       4.    Viva voce                                                                                                                                   05  

       5.    Journal                                                                                                                                       05   

 

PRACTICAL EXAMINATION PSZOOCN4P4 

                                                                                                      Total Marks: 50   

   

1. Biometric study of fish 

(A)  Study of relationship between total length and standard length / head length / body 

depth length / body weight                                                                                                                                

04 

(B)  Calculate correlation (standard length and total length / head length and total length      

03 

2. Preparation of zooplankton mounting (5 different mounting of zooplankton)                            

10  

              3.    Identification ( 1 from fouling and boring organism, 1 from fresh water fish & fresh water     

prawn – 1 from crustacean fishery, 1 from molluscan fishery)   ( 4 X 2 marks each)                   

08                                                                                                                                                               

4.   (A) Herbarium                                                                                                                                             

05                        (B) Field report (visit to aquaculture centre, boat building yards, processing 

plants, marine biological Institutions)   ( Excursion or Study tours)                                                                          

04 

                     (C)  Collection molluscan shells ( 5 shells )                                                                                           

04                     

                     (D) Report on shrimp prawn pickle                                                                                                        

02 

              5.     Viva voce                                                                                                                                                   

05 

              6.     Journal                                                                                                                                                       

05   

 

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