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MODULE-9
SEX DETERMINATION
9.1 ANIMALS
G E N E T I C S-I
• Define the term sex chromosome vs. autosomal chromosomes.
• Describe the sex determining mechanisms in the animal kingdom – sex chromosome mediated methods viz: XY-XX; XO-XX; ZW-ZZ; Z0-ZZ .
• Describe the Chromosome Balance Theory of Sex Determination; haplo-diploidy; haplo-diploidy and complementary genes; genic control of sex.
G E N E T I C S-I LEARNING OUTCOMES
• Explain the following mechanisms of sex determination using appropriate examples to illustrate: – (a) Mammalian XY-XX system – (b) Protenor XX-XO system– (c) Birds ZW-ZZ system– (d) ZO-ZZ system– (e) Sex Balance Theory (genic balance)– (f) Yeast (MATa – MATα mating types)– (g) Environmental sex determination
G E N E T I C S-I LEARNING OUTCOMES
X Y X
XX XY½ ½
XY x XX
♂ ♀
Lygaeus systemMost common, e.g. humans, rats, some reptiles-green iguana
Sex determination in the animal kingdom
G E N E T I C S-I
Females: two X-chromosomes, homogametic.
Males: single X-chromosome and Y-chromosome, heterogametic.
Sex determination in the animal kingdom (humans)
G E N E T I C S-INormal humans
Some human abnormalities
SRY is a Y-linked gene and codes for testis-determining factor (maleness).
X O X
XX XO½ ½
XO x XX
♂ ♀
Protenor systemInsects: Hemiptera, Orthoptera
Sex determination in the animal kingdom
G E N E T I C S-Igrasshoppers
Females: two X-chromosomes, homogametic
Males: single X-chromosome, heterogametic
O is not a chromosome.
Z W Z
ZZ ZW½ ½
ZZ x ZW
♂ ♀
Birds, moths, some isopods, some amphibians and some fishes
Sex determination in the animal kingdom
G E N E T I C S-IFemales: one Z and one W chromosome, heterogametic
Males: two Z chromosomes, homogametic
The chromosomes are not shaped like Z or W, just to differentiate from XY system.
Z O Z
ZZ ZO½ ½
ZO x ZZ
♂♀
Butterflies, caddies flies
Sex determination in the animal kingdom
G E N E T I C S-I
Females: one Z chromosome, heterogametic
Males: two Z chromosomes, homogametic
O is not a chromosome.
Lygaeus system (XY) - homogametic sex is female - heterogametic sex is male - Sex ratio is 1:1
Protenor system (XO – XX) - homogametic sex is female - heterogametic sex is male - Sex ratio is 1:1
ZO system - homogametic sex is male - heterogametic sex is female
ZW system - homogametic sex is male - heterogametic sex is female
Sex determination in the animal kingdom
G E N E T I C S-I
Sex determination in the animal kingdom (environment)
G E N E T I C S-IMarine mollusk (Crepidula fornicata)- common slipper limpet.
•Live in stacks- one on top of the other
•First to settle on a solid unoccupied substrate develops into a female.
•Those that settle on top become males and serve as mates for the female.
•After a while the males turn into females and attract more male larvae on top of them.
•This is referred to as sequential hermaphroditism- each animal can be both male and female, although not at the same time.
Sex determination in the animal kingdom (environment)
G E N E T I C S-I• Some reptiles- temperature during embryonic development determine
sexual phenotype.
• Turtles- warm temperatures produces females; cool temperatures produce males.
• Alligators- warm temperature produces males (>34oC); cool temperatures produce females (<30oC).
Effect of climate change?
Chromosome balance theory of sex determination (Genic balance) in Drosophila melanogaster (fruit fly)
- Sex is determined by the balance between sex chromosomes and autosomal complement of chromosomes
- Lets say that ‘A’ represents a haploid set of autosomal chromosomes X/A ratio = 1 or higher = female X/A ratio = 0.5 or lower = male X/A ratio = >0.5 - <1.0 = intersex
The genome of ♂ fly = AAXY The genome of ♀fly = AAXX
-Y chromosome is involved in male fertility but not in the determination of sex
G E N E T I C S-I
Evidence for the Genic balance theory in Drosophila melanogaster Bridges (1922) observed that Drosophila occasionally produces a triploid
due to non-disjunction.
AX AY AX
AAXX AAXY½ ½
AAXY x AAXX
♂ ♀
AX AY AAXX
AAAXXX AAAXXYX/A = 1 X/A = 0.66
AAXY x AAXX
♂ ♀
G E N E T I C S-I
AX AY AAX
AAXY x AAAXXX
♂ ♀
AXX
AAAXX AAXXX AAAXY AAXXY
X/A =0.6 X/A =1.5 X/A = 0.33 X/A = 1
INTERSEX
SUPERFEMALE
SUPERMALE
FEMALE
Male not fertile Male fertile
Evidence for the Genic balance theory in Drosophila melanogaster
G E N E T I C S-I
Intersex (Gynandromorphs) in Drosophila melanogaster:
- Have male and female organs - Highly influenced by envt - high To – more female - low To – more male - Modifying factors also influence
- more male or female
G E N E T I C S-I
Superimposed on the genic balance system is another level of control of the determination of sex - transformer gene (autosomal gene)
This gene in the homozygous recessive state (tra. tra) converts a diploid female into a sterile male.
AAXX Sterile Male
AAXX Female
tra.tra
Tra.__
Transformer gene sex determination in Drosophila melanogaster
G E N E T I C S-I
Transformer gene
Genic Balance
Overall sex determination in Drosophila melanogaster
G E N E T I C S-I
Autosomes alone are involved.
The number of autosomal complements determine sex.
e.g. Hymenopteran insects such as bees, wasps, antsIn Bees - eggs haploid males (A)
Pathenogenesis (arrhenotoky)
FertilisationDiploid females (AA)
Quantity of food
Queen Female worker
(worker)
The queen controls sex ratio
Haplo-diploidy sex determination
G E N E T I C S-I
e.g. Hymenopteran insects such as bees, wasps, ants
In Wasps - eggsPathenogenesis (arrhenotoky)
Fertilisation
Diploid female worker
haploid males (A)(worker)
Diploid male workerHomozyous gene ‘S’
Heterozygous gene ‘S’
Haplo-diploidy + complementary gene method of sex determination
G E N E T I C S-I
The ‘S’ gene has 9 multiple alleles Sa, Sb, Sc, Sd….Si
Homozygous states
SaSa, SbSb, ScSc, SdSd…… SiSi = diploid worker males
Heterozygous states
SaSb, SaSc, SaSd etc = diploid worker females
There are more heterozygous combinations than homozygous and hence more worker females than worker males
G E N E T I C S-IHaplo-diploidy + complementary gene method of sex determination
Lower plants such as chlamydomonas and fungi- one or a few autosomal genes control sex.
e.g. Chlamydomonas - ‘A’ = + mating type = - mating type
e.g Basidiomycete fungi – two genes, A & BAB – mating type-1Ab – mating type-2aB - mating type-3ab – mating type-4
Sex determination- one or few autosomal genes (genic sex determination)
G E N E T I C S-I
Males and females look similar.
Fungi , lower plantsSingle or few autosomal genes
InsectsWasps Entire autosomal complement + single geneBees Entire autosomal complementDrosophila Autosomes + sex chromosomes
Higher animals Sex chromosome mediated methods- XY, XO, ZW, ZO- environment
G E N E T I C S-I
SUMMARY